华南虎、金钱豹、云豹血清蛋白和LDH同工酶的电泳研究     

杨丽娥  王保青  张建平 《上海交通大学学报(农业科学版)》1993,(2)

本文报道了华南虎、金钱豹、云豹的血清蛋白和LDH同工酶的聚丙烯酰胺凝胶电泳图谱和扫描曲线。分析结果表明:华南虎、金钱豹和云豹的血清蛋白电泳可分辨的区带数分别为13、14、13。在各区带的相对迁移率及含量方面显示出动物体蛋白在物种间存在着差异。3种动物血清LDH同工酶谱带均为5条,但仍各具特征性电泳图谱,且LDH同工酶表型分析结果提示,华南虎与金钱豹的电泳图谱较为接近。  相似文献   

5种桉属植物的miRNA生物信息学预测     

李崇奇  周鹏  蔡望伟 《安徽农业科学》2014,(15):4587-4590

[目的]识别桉树miRNA基因和寻找与桉树木质形成相关的miRNA,为进一步开展桉树遗传品质改良工作奠定理论基础。[方法]将mirBase数据库中所有植物的3 228条miRNA序列提交到psRobot网站进行茎环结构预测,应用blast-2.2.27＋软件与rfam数据库和pfam数据库比对后去除非miRNA序列,并应用bioedit软件分析miRNA序列及前体序列的碱基组成特点。[结果]共计预测到26条miRNA前体序列和19条不同的成熟miRNA序列,并发现桉树miRNA序列在碱基偏倚现象,5’端第1碱基尿嘧啶出现的频率高达52.6%,而在第19碱基胞嘧啶出现频率为61.1%。靶基因预测发现3个与木质形成相关的miRNA。[结论]应用生物信息学方法首次在桉树中预测到了19条成熟miRNA序列,并识别到了3个与木质形成相关的miRNA。  相似文献   

Tissue and cell culture as aids to sugarcane breeding. II. Performance and yield potential of callus derived lines   总被引：1，自引：0，他引：1  

Ming -Chin Liu  Wen -Huei Chen 《Euphytica》1978,27(1):273-282

Summary Thirteen callus derivatives from the sugarcane (Saccharum sp. hybrid) varieties F156 and F164 were studied to investigate the potential of tissue culture methods to generate superior germplasm for plant breeding in a field trail conducted at a single location. Comparisons between callus derivatives and donors were made for 9 characters, viz. cane yield, sugar yield, stalk number, length, diameter, volume, density, weight/stalk, and percent fiber. Statistical analyses were run, which involved stepwise multiple regression, broad-sense heritability (H), genetic advance (G). Esterase zymogram of superior callus derivatives was examined. 70–6132 was 32, 34 and 6% higher than its donor (F164) in cane yield, sugar yield and stalk number, respectively. The differences between them for the first two characters reached the 5% probability level. 70–6132 was also higher for these 3 characters than F160, the No. 1 commercial variety, by 20, 16 and 8%, respectively. 70–6136 ranked next only to 70–6132. Its grand means for these 3 characters outdid the parental (F164) means by 21, 24 and 3%, respectively. 70–6069 was significantly higher in stalk diameter and volume than most of the callus derivatives but not higher than its donor (F156). The performances of other derivatives were either similar or inferior to both their donors and F160. Stalk number, stalk length and weight/stalk accounted for 43, 29 and 18% of the variation of cane yield, respectively. H values for the 7 characters (stalk density and percent fiber were not included) ranged from 31 (stalk length) to 66% (stalk volume). G/\-x 100 for the 7 characters ranged from 3.3 (stalk length) to 18.6% (sugar yield). 70–6132 had two bands missing, 70–6069 had two bands more whereas those of 70–6136 showed no visible change, in esterase zymogram as compared with the donors. Due to the improved yield and the genetic changes revealed by the isoenzyme patterns in callus derived lines, tissue culture methods can be used to create superior varieties for agricultural use.  相似文献   

Location of a Gene for Resistance to Eyespot (Pseudocercosparella herpotrichoides) on Chromosome 7D of Bread Wheat     

A. J. Worland    C. N. Law    T. W. Hollins    R. M. D. Koebner    A. Giura 《Plant Breeding》1988,101(1):43-51

Chromosome 7D of the wheat line VPM1 derived from a cross of Aegilops ventricosa with wheat confers resistance to the facultative fungal parasite Pseudocercosporella herpotrichoides. To determine the number of genes responsible fur this resistance, homozygous recombinant lines were developed from an F₁ between the wheat variety ‘Hobbit sib’ and a substitution line carrying chromosome 7D of VPM1 in a ‘Hobbit sib’ background. Resistance to Pseudocercosporella herpotrichoides is shown to be determined by a single gene located distally on the long arm of chromosome 7D. EpD1b, a unique allele of a gene encoding the readily detectable isoenzyme — endopeptidase, maps without recombination to Pch1 suggesting for two separate genes a maximum recombination value of 0.03 (P 0.05). Resistance to Pherpotrichoides could alter-natively be a product of Ep-D1b. Pch1 is also mapped against a gene for adult plant resistance to brown rust (Puccinia recondita), to Rc3 which confers coleoptile colour, and to α-Amy-D2, an isozyme that encodes α-amylase production.  相似文献   

Characterization of novel sugarcane expressed sequence tag microsatellites and their comparison with genomic SSRs   总被引：6，自引：0，他引：6  

L. R. Pinto    K. M. Oliveira    T. Marconi    A. A. F. Garcia    E. C. Ulian    A. P. de Souza   《Plant Breeding》2006,125(4):378-384

Microsatellites or simple sequence repeats (SSRs) are one of the most suitable markers for genome analysis as they have great potential to aid breeders to develop new improved sugarcane varieties. The development of SSR derived from expressed sequence tags (EST) opens new opportunities for genetic investigations at a functional level. In the present work, the polymorphism obtained with a subset of 51 EST–SSRs derived from sucest was compared with those generated by 50 genomic SSRs (gSSR) in terms of number of alleles, polymorphism information content, discrimination power and their ability to establish genetic relationships among 18 sugarcane clones including three Saccharum species (S. officinarum, S. barberi, S. sinense). The majority of EST–SSRs loci had four to six alleles in contrast to the seven to nine observed for the gSSRs loci. Approximately, 35% of the gSSRs had PIC values around 0.90 in contrast to 15% of the EST–SSRs. However, the mean discrimination power of the two types of SSR did not differ significantly as much as the average genetic similarity (GS) based on Dice coefficient. The correlation between GS of the two types of SSRs was high (r = 0.71/P = 0.99) and significant. Although differences were observed between dendrograms obtained with each SSR type, both were in good agreement with pedigree information. The S. officinarum clone IJ76‐314 was grouped apart from the other clones evaluated. The results here demonstrate that EST–SSRs can be successfully used for genetic relationship analysis, extending the knowledge of genetic diversity of sugarcane to a functional level.  相似文献   

The characterization and assessment of genetic diversity among maize (Zea mays L.) hybrids that are widely grown in France: Chromatographic data and isozymic data     

J. S. C. Smith 《Euphytica》1989,43(1-2):73-85

Summary Zein chromatographic and isozyme electrophoretic data were used to characterize 61 hybrids of maize (Zea mays L.) that are of current widespread usage in France. A similar study was also carried out for 88 hybrids that are widely available in the central corn belt of the U.S. Objectives were to 1) investigate the degree to which isozyme and chromatographic data could provide unique hybrid identification; 2) to evaluate the wealth of genetic diversity available among hybrids that are currently cultivated in France and in the U.S.; and 3) to compare the patterns and extent of genetic diversity found in both countries with respect to their registration, breeding, and seed production practices.Isozyme data showed that all French hybrids were unique germplasm. However, numerous hybrids had chromatographic profiles that were identical for 11 major peaks and these hybrids could thus have some common germplasm at least among their female parental lines. Although there was a broad base of isozymic diversity among French hybrids, the amount in cultivation was less since the few most widely used hybrids, which were planted on approximately half the cultivated area, were similar for their isozymic and zein chromatographic profiles. Approximately only 50% of the U.S. hybrids were shown to be unique with the remainder classified into groups; at least some of the hybrids within each group could be genetically identical products. Leading U.S. hybrids were unique germplasm but numerous hybrids that collectively covered a large acreage appeared to encompass a relatively narrow genetic base.For the continued success of agriculture, it will be important to improve breeding, registration, and marketing practices in order to ensure the continued supply of genetically different and improved elite hybrids.  相似文献   

应用酯酶同工酶分类鉴定平菇菌种的研究     

李喜梅  贾新成 《河南农业大学学报》1989,(1)

采用聚丙烯胺凝胶园盘电泳法对河南省表现较好的38个平菇菌株的菌丝体进行酯酶同工酶的研究,结果发现38个菌株的同工酶谱分为12个类型。试验表明,根据酯酶同工酶谱的类型对平菇菌种进行分类是一种简单迅速而又可靠的鉴别分类方法。  相似文献   

锰对大豆POD和EST同工酶谱的影响     

徐根娣  孙和和  刘鹏  包海英 《河南农业科学》2006,(4):46-50

采用垂直平板聚丙烯酰胺凝胶电泳,研究了2个大豆品种(华春18和浙春3号)在不同时期、不同锰浓度处理下的过氧化物(POD)同工酶和酯酶(EST)同工酶的变化情况。结果表明,随锰浓度的增加,华春18的POD同工酶的酶带数下降,EST同工酶的酶带数变化不大,浙春3号的2种同工酶的酶带数变化不大;2个大豆品种在不同时期的POD和EST同工酶的酶带数也变化不一,这与各品种对锰胁迫的抗性有关。  相似文献   

草菇杂交育种及同工酶分析   总被引：1，自引：0，他引：1  

谢宝贵  羿红  黄志龙  林友照  谢福泉  江玉姬 《福建农林大学学报(自然科学版)》2001,30(3):372-376

选用 10个草菇菌株作为亲本 ,相互交配获得 4 5个杂交菌株 .品比试验结果表明 :大多数高产杂交组合的两个亲本都具有较高的一般配合力 ,双亲间又具有较高的特殊配合力 ;单孢菌株普遍表现为出菇晚、产量低 ;不论是亲本、单孢菌株还是杂交菌株 ,出菇早的菌株普遍产量较高 ,出菇晚的产量低 .同工酶分析结果表明 ,h0 4、h32、h33是杂交种  相似文献   

小麦叶片衰老过程中CAT和APX活力及其同工酶谱的变化   总被引：13，自引：0，他引：13  

冯晴  徐朗莱 《南京农业大学学报》1997,20(2):95-99

小麦叶片在连体自然衰老和离体诱导衰老过程中，过氧化氢酶（ＣＡＴ）活力比抗坏血酸过氧化物酶（ＡＰＸ）活力下降得早。当ＣＡＴ活力处于较低水平时，ＡＰＸ仍然维持较较活力，且ＡＰＸ活力与Ｈ２Ｏ２含量呈显著负相关，经同工酶电泳分析，离体叶片暗诱导衰老过程中，ＣＡＴ同工酶带无增减，只是活性免疫，而ＡＰＸ同工酶带则有增加，ＡＰＸ在小麦叶片衰老的中后期可能起着比ＣＡＴ更重要的清除Ｈ２Ｏ２的作用。  相似文献