Polymorphism in promoter region of growth hormone receptor is associated with potential production capacity of insulin‐like growth factor‐1 in pre‐pubertal Holstein heifers          下载免费PDF全文

C. Kawashima  M. Munakata  M. Matsui  A. Miyamoto  K. Kida  T. Shimizu 《Journal of animal physiology and animal nutrition》2016,100(6):1037-1040

Insulin‐like growth factor‐1 (IGF‐1) is one of the important factors for growth, milk production and reproductive functions and mainly released from the liver in response to growth hormone (GH) via GH receptor (GHR) in cattle. Recently, some single nucleotide polymorphisms (SNPs) were identified in the bovine GHR gene. Some GHR‐SNPs were shown to be related to plasma IGF‐1 concentration in cattle. Hence, the capacity to IGF‐1 production in the liver might be affected by GHR‐SNP and associated with performance in the future. This study examined whether GHR‐SNP is associated with IGF‐1 production in the liver of pre‐pubertal heifers. In 71 Holstein calves, blood samples for genomic DNA extraction were obtained immediately after birth. To genotype the GHR‐SNPs in the promoter region, polymerase chain reaction (PCR) products were digested with restriction enzyme NsiI (cutting sites: AA, AG and GG). All heifers at 4 months of age were intramuscularly injected with 0.4 mg oestradiol benzoate. Blood samples were obtained from the jugular vein just before (0 h) and 24 h after injection. The number of AA, AG and GG at the NsiI site was 0, 17 and 54 respectively. In AG and GG, plasma GH concentrations were higher pre‐injection than 24 h post‐injection (p < 0.01). Moreover, plasma GH concentrations in AG post‐injection were higher than in GG (p < 0.05). In contrast, the GG genotype exhibited higher plasma IGF‐1 concentrations in pre‐injection than post‐injection (p < 0.01), although oestradiol did not change IGF‐1 concentration in the AG genotype. We conclude that the GG polymorphism in the promoter region of GHR is associated with a higher potential capacity of IGF‐1 production in the liver of cattle.  相似文献   

Cyclooxygenase (COX) inhibitors and the intestine   总被引：1，自引：0，他引：1  

Little D  Jones SL  Blikslager AT 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(3):367-377

Nonsteroidal anti-inflammatory drugs (NSAIDs) have long been used for the treatment of pain and inflammation because of their inhibitory effects on cyclooxygenase (COX). For almost as long as NSAIDs have been in use, multiple adverse effects have been noted. Assessment of many of these adverse effects have been complicated because of the discovery of multiple splice variants of the cox gene, and a greater array of COX inhibitors, especially the COX-2 selective inhibitors have become available. Some of these adverse effects cannot be readily explained by the effect of these drugs on COX. This has sparked a new field of investigation into the COX-independent effects of the COX inhibitors. The major noncyclooxygenase targets of the COX inhibitors of particular relevance to inflammation and the gastrointestinal tract are phosphatidylinositol 3'-kinase Akt signaling, uncoupling of oxidative phosphorylation, PPARgamma, nuclear factor KB, mitogen activated protein kinases, and heat shock proteins.  相似文献   

沉默蜕皮激素受体对雌性中黑盲蝽生殖力的影响     

黄星星  郑宛莹  薛汇  焦盼阳  陈利珍 《植物保护》2023,49(1):164-177

中黑盲蝽Adelphocoris suturalis是一种重要的农业害虫,主要为害棉花、果树和牧草等作物。昆虫保幼激素(juvenile hormone, JH)与蜕皮激素(molting hormone, 20E)是调控昆虫生殖的主要因素。本研究运用基因克隆、基因沉默(RNAi)、实时荧光定量PCR(qPCR)等技术研究蜕皮激素受体(ecdysone receptor, EcR)、超气门蛋白(ultraspiracle protein, USP)在中黑盲蝽生殖调控中的作用。结果表明,中黑盲蝽EcR基因的ORF全长1 413 bp,编码470个氨基酸。预测蛋白质分子量为53.65 kD,pI值为7.79。中黑盲蝽USP基因ORF全长1 209 bp,编码402个氨基酸。预测蛋白质分子量为44.99 kD, pI值为7.94。与对照相比,注射dsEcR、dsUSP后6～18 d的中黑盲蝽体内EcR基因、USP基因在转录水平分别被显著抑制;卵巢的挂卵量分别仅有对照组的31.5%、86.6%;单雌产卵量减少36.0%～80.4%,显著低于对照。EcR和USP基因沉默后,产卵前期与对照相比无显...  相似文献   

Correlation between progression of atherosclerosis accelerated by emotional stimulation and imbalance of SDF-1/CXCR4 in mice     

HUANG Zeng-yan  HUANG Man-ping  PENG Chun-li  LIU Yong-yuan  LI Chun-yan  LIANG Dong-hui 《园艺学报》2021,37(1):34-40

AIM To observe effects of emotional stimulation on expression of stromal cell-derived factor-1（SDF-1） and CXC chemokine receptor 4 （CXCR4） in plasma, platelets, aortas, hippocampus and bone marrow of apolipoprotein E gene knockout （ApoE^-/-） mice, and to reveal the possible mechanism of the aggravated atherosclerotic plaque vulnerability by emotional stimulation. METHODS Thirty 8-week-old male ApoE^-/- mice were randomly divided into normal control group, high fat group, and emotional stimulation group. Ten 8-week-old inbred C57BL/6J mice served as blank control group. After 12 weeks of intervention, the serum levels of SDF-1 and CXCR4 were investigated by ELISA. The protein levels of SDF-1 and CXCR4 in platelets, aortas, hippocampus and bone marrow were determined by Western blot. The pathological damage of aortas was observed by oil red O staining. RESULTS Compared with blank control group, normal control group and high fat group, the mice subjected to emotional stimulation showed more serious atherosclerosis in aortas detected by oil red O staining, and increased levels of SDF-1 and CXCR4 in the plasma and aortas were also observed （P<0.05）. The results of Western blot showed that the protein levels of SDF-1 and CXCR4 in platelets, aortas and hippocampus were increased in the mice subjected emotional stimulation, but the expression of SDF-1 and CXCR4 in the bone marrow was decreased （P<0.05）. CONCLUSION Imbalance of SDF-1/CXCR4 may be the key target by which emotional stimulation accelerates the progression of atherosclerosis.  相似文献   

卵泡刺激素受体基因作为产仔数候选基因的研究   总被引：8，自引：1，他引：8  

张淑君  熊远著  邓昌彦  郑嵘  蒋思文  肖森木  夏瑜  徐建祥  刘晓华  王春芳  阮征  宫时玉 《华中农业大学学报》2002,21(6):506-508

分析了FSHR基因2个位点（FSHRA、FSHRB）的PCR－SSCP、PCR－RFLPs多态性，利用SAS软件分析了多态性与母猪产仔数间连锁关系。找到了1个影响产仔数的新的变异位点FSHRB，该位点多态性显著地影响猪产仔数性状，即基因型BB的产仔数显著地高于AB、AA基因型。结果显示出该位点具有作为常规选育的辅助选择的有效的分子标记潜力。  相似文献   

A role for Toll-like receptor 4 in the host response to the lung infection of Yersinia pseudotuberculosis in mice     

《Comparative immunology, microbiology and infectious diseases》2016

Although a Yersinia pseudotuberculosis (Yptb) lung infection model has been developed to study Y. pestis pathogenesis, it is still necessary to establish a new animal model to mimic the pathophysiological features induced by Y. pestis infection. Here, we provide a new lung infection model using the Yptb strain, IP2777, which displayed rapid spread of bacteria to the liver, spleen, and blood. In addition, we examined whether TLR4 is involved in Yptb-induced pathogenesis in the lung infection model of mice we generated. Following lung infection of WT and TLR4-deficient mice with the Yptb strain IP2777, the survival rate, bacterial colonization, histopathology, and level of cytokines and chemokines in the lung, spleen, liver, and blood were analyzed. TLR4-deficient mice had a lower survival rate than WT mice in response to Yptb lung infection. Although the bacterial colonization and pathology of the lung were comparable between WT and TLR4-deficient mice, those of the spleen and liver were more severe in TLR4-deficient mice. In addition, the levels of TNF-α and CXCL2 in the liver and IL-6 and CXCL2 in the blood were higher in TLR4-deficient mice than in WT mice. Our results demonstrate that TLR4 is necessary for optimal host protection against Yptb lung infection and TLR4-deficient mice may serve as a better genetic model of Yptb infection for mimicking Y. pestis infection.  相似文献   

ER-α36 and miR-143 involved in invasion of gastric cancer cells     

ZOU Feng  WANG Xu-ming  LIU Li-jiang 《园艺学报》2012,28(12):2167-2171

AIM: To investigate the relationship between the expression of estrogen receptor α36 (ER-α36) and the invasion of gastric cancer cells. METHODS: SGC7901 cells were treated with 17β-estradiol at high or low concentration. The invasion of gastric cancer cells and the expression of ER-α36 were detected. The SGC7901 cells with the characteristics of stable low expression and high expression of ER-α36 were constructed. The invasion ability and microRNA sequences were determined in above-mentioned recombinant cells. RESULTS: The decreased invasion ability and ER-α36 expression were detected in SGC7901 cells treated with low concentration of 17β-estradiol. The situation was the opposite in the cells treated with high concentration of 17β-estradiol. The expression of miR-143 was significantly decreased in the SGC7901 cells with stable high expression of ER-α36 and was increased in the SGC7901 cells with stable low expression of ER-α36. CONCLUSION: The expression of ER-α36 is positively related to the invasion of gastric cancer cells. It is possible that miR-143 plays an important role in the regulation of gastric cancer invasion.  相似文献   

Atorvastatin inhibits IL-1β and IL-18 releases via lowering the expression of NLRP1 inflammasome     

YU Si-yang  WANG Yan  ZENG Gao-feng  LIU Yang  XU Jian-qiang  ZENG Meng-ya  TANG Ye-hua  ZENG Zhi-ying  SHI Xiao-qiao  CHEN Ying  ZHAO Guo-jun 《园艺学报》2016,32(5):863-868

AIM: To explore the role of nucleotide-binding oligomerization domain-like receptor protein 1 (NLRP1) inflammasome in atorvastatin-induced reduction of interleukin-1β (IL-1β) and interleukin-18 (IL-18) releases from the THP-1 macrophages. METHODS: Lipopolysaccharide (LPS, 10 μg/L) was used to trigger the secretion of IL-1β and IL-18 in the THP-1 macrophages. The cells were incubated with different concentrations of atorvastatin (1, 10 and 20 μmol/L) for 24 h, or treated with 10 μmol/L atorvastatin for different time (12 h, 24 h and 48 h). NLRP1 siRNA was transfected into the THP-1 cells. The mRNA expression of NLRP1 inflammasome was detected by RT-PCR. The protein expression of NLRP1 inflammasome was determined by Western blot. The secretion of proinflammatory cytokines IL-1β and IL-18 was quantified by ELISA. RESULTS: Atorvastatin inhibited the mRNA and protein expression of NLRP1 inflammasome in the THP-1 macrophages in a dose- and time-dependent manner. Transfection of NLRP1 siRNA significantly decreased the protein expression of NLRP1 and promoted the suppressive effect of atorvastatin on IL-1β and IL-18 secretion in the THP-1 macrophages. CONCLUSION: Atorvastatin inhibits the production of IL-1β and IL-18 in the macrophages through decreasing NLRP1 inflammasome expression, possibly contributing to the anti-inflammatory effect of atorvastatin on atherosclerosis.  相似文献   

Interaction of NR2D subunit of NMDA receptor with MOCA     

BAI Ning  YI Fei  LIU Ting  LI Xiao-man 《园艺学报》2016,32(6):1118-1121

AIM: To identify the potential proteins interacting with NR2D subunit of NMDA receptor by yeast two-hybrid screening and to investigate the role of NR2D in excitotoxicity of the retina.METHODS: The Clontech GAL4 yeast two-hybrid system was used to screen the mouse brain cDNA library, and the bait plasmid containing C-terminus of NR2D was constructed. Physical interaction between 2 proteins was verified by co-immunoprecipitation assay. The subcellular localization of 2 proteins in the mouse retina was observed under microscope with immunofluorescence.RESULTS: Modifier of cell adhesion (MOCA) was identified as a new protein interacting with NR2D. MOCA and NR2D were co-expressed in the mouse retina. CONCLUSION: MOCA specifically interacts with NR2D, which provides the experimental basis for identifying the role of glutamate excitotoxicity in the retina neurodegeneration.  相似文献   

动物鲜味受体的研究进展及其基因表达调控     

王晶  张勇  黄铁军 《动物营养学报》2016,(9):2702-2708

动物的鲜味受体包括代谢型谷氨酸受体(m GluR)和味觉受体异源二聚体(T1R1/T1R3),是C型G蛋白偶联受体,N末端捕蝇草模块(VFT)区域可与鲜味配体结合,识别鲜味。本文主要论述了鲜味受体的研究进展、鲜味识别转导机制及鲜味受体基因的表达调控等,以期为相关研究提供参考。  相似文献