中华真地鳖抗菌物质的抑菌活性测定     

索相敏  陆秀君  董建臻  李静  宋萍  赵瑞君  付利燕 《中国生物防治》2007,23(1):64-67

以研磨法从中华真地鳖五龄若虫体内提取得到抗菌物质,分别采用比浊法和菌碟法测定了其对多种细菌和植物病原真菌的抑菌活性。结果表明,该抗菌物质对大肠杆菌、金黄色葡萄球菌、白菜软腐菌和枯草芽孢杆菌等4种细菌生长均有一定的抑制作用,对数期延迟1~4h,菌的浓度、繁殖速度减缓。对7种植物病原真菌的抑菌试验表明,该抗菌物质对玉米茎基腐病菌、小麦根腐病菌、棉花枯萎病菌抑制效果较好,抑制率分别为50.0%、49.2%、45.2%;而对白菜黑斑病菌和玉米大斑病菌活性较差,抑制率仅为28.3%和24.5%。  相似文献   

草莓休眠过程中内源激素含量的变化   总被引：19，自引：2，他引：17  

葛会波  李青云 《园艺学报》1998,25(1):89-90

研究了草莓休眠过程中,内源ＡＢＡ、ＧＡ３、ＩＡＡ和ＺＴ（玉米素）含量变化。结果表明,同种内源激素在露地和保护地栽培条件下变化趋势相似。深休眠和浅休眠品种同种内源激素含量变化趋势相似,但含量变化在时间上有所差异。  相似文献   

Effect of Synthetic Cytokinin Application on Pod Setting of Individual Florets within Raceme in Soybean     

《Plant Production Science》2013,16(2):79-81

Abstract

The application of synthetic cytokinin (6-benzylaminopurine, BA) to racemes of soybean genotype IX93-100 at 7 days after anthesis (DAA) enhanced pod-set percentage of the florets at the 5th position and above (numbered from the base on rachis). The endogenous cytokinin (trans-zeatin riboside) content of individual florets was measured at the 1, 3, 5, 7th position every 3 days after anthesis. Cytokinin was detected only from the florets at 9 DAA, and the content was higher in the more proximal florets while it became negligible in the 7th floret. These results suggest that an increase in the amount of cytokinin in individual florets might enhance the pod setting of the florets positioned at the middle or distal part within the raceme.  相似文献   

抑制鹿源多药耐药结核菌中药的筛选   总被引：1，自引：0，他引：1  

姜园园  李温明  刘畅  曾范利  时坤  李健明  刁乃超  李仲乐  刘东旭  杜锐 《动物医学进展》2016,(4):54-57

为了筛选对鹿源多药耐药结核菌有效的中药耐药抑制剂,通过MABA法检测24种中药的水和乙醇提取物对多药耐药结核菌及标准菌H37Rv的体外抗菌效果,将筛选出的抗菌效果好的黄连醇提物和黄连水提物采用紫外分光光度法进行活性成分含量测定,同时以MABA法与标准品比较体外抗结核菌活性。结果显示,黄连、独活和侧柏叶有明显的抗结核活性,其中黄连水粗提物和乙醇粗提物效果最佳,测得它们活性成分即总生物碱含量分别为22.74%和35.23%,并显示二者与小檗碱的体外抗结核作用相同。  相似文献   

核桃楸枝条,插穗中生根抑制物质的含量   总被引：9，自引：0，他引：9  

徐程扬  张忠辉 《吉林林学院学报》1998,14(4):212-215

通过红心萝卜种子发芽试验表明核桃楸母树体内存在着生根抑制物质．枝皮内的生根抑制物质含量随母树年龄的增加而提高；生根抑制物质含量在高生长旺盛期枝皮内最低，在休眠期含量最高；平茬具有较强的生理复幼作用，根萌枝条皮内的生根抑制物质含量较低；插穗在扦插过程中皮内生根抑制物质含量逐渐降低．  相似文献   

硝化抑制剂缓释尿素试验效果初探   总被引：3，自引：0，他引：3  

李冬梅  王绍新  刘芳  柳维刚  初艳萍 《北方水稻》2008,38(3):96-97

硝化抑制剂缓释尿素具有提高氮素利用率、增产、增收、节肥等功效。为验证其在水稻上的应用效果,特进行此试验。结果表明:底肥一次性施用硝化抑制剂缓释尿素或第一代缓释尿素对水稻具有不同程度的增产效果,分别增产6.7%、3.9%,净增收76.35元/667 m2、52.19元/667 m2,节省肥料、节省功时费,在生产上有一定推广价值。  相似文献   

Increase of in vitro regeneration in Malus domestica by the application of phosphatase inhibitors   总被引：1，自引：0，他引：1  

C. Graph  G. Wricke 《Plant Breeding》1998,117(6):563-566

The effect of the phosphatase inhibitors sodium fluoride and sodium orthovanadaate on adventitious shoot regeneration from primary leaf explants was tested in eight genotypes of apple Malus domestica Borkh. Ten different concentrations between 0.5 and 100μM of each substance were added to a modified Murashige and Skoog regeneration medium containing 0.9 μM TDZ and 0.5 μM IBA. In general, all the con-centrations tested were able to promote shoot formation, although distinct maxima could be observed depending upon the genotype and the inhibitor. Shoot yield was influenced mostly by the genotype and by the concentration of the inhibitor, as well as the interaction between these two factors. In six of the eight genotypes the total number of regenerated shoots increased significantly compared with the control. This was achieved by an increase of the number of regenerating explants as well as the number of shoots per regenerating explant Comparing these results with those of former publications, application of the two phosphatase inhibitors resulted in an improved regeneration response in the corresponding genotypes. This method may also contribute to the understanding of in vitro regeneration in plants.  相似文献   

Alpha-amylase/subtilisin Inhibitor Levels in Australian Barleys     

S.J Jarrett  RJ Marschke  M.H Symons  C.E Gibson  R.J Henry  G.P Fox 《Journal of Cereal Science》1997,25(3):261-266

An enzyme-linked immunosorbent assay (ELISA) was used to determine the bifunctional alpha-amylase/subtilisin inhibitor (BASI) content of barley grain from 11 cultivars grown in six diverse locations in Australia. The inhibitor ranged from 119 to 254 μg/g in 57 barley samples. Genotype had a significant (P<0·05) effect on BASI content but there was no effect due to environment. Total protein varied independently of BASI and was influenced by environment and genotype. BASI content was higher (P<0·05) in malting barley than in feed barley and was correlated positively (r=0·29;P<0·05) with alpha-amylase activity in corresponding malts. The ELISA used monoclonal and polyclonal antibodies raised against purified BASI. In immunoblot analysis the monoclonal antibody showed high specificity for the inhibitor in barley and also detected the inhibitor in wheat. Low levels of inhibitor (mean 3·2 μg/g) were found in 12 Australian wheat cultivars using the ELISA developed for barley. The assay had a linear working range of 5–50 ng/mL with a detection limit of 2 ng/mL. Reproducibility between assays was good (CV=4·9%) but mean recoveries were high, ranging from 116–129% when purified inhibitor was added to barley extracts. The ELISA may have useful applications in brewing research and barley breeding programmes.  相似文献   

缺失SBTi—A2大豆杂交后代品系籽粒化学成分分析     

孟祥勋  赵述文 《大豆科学》1997,16(2):174-177

对一个杂交组合后代１０个稳定缺失胰蛋白酶抑制剂（ＳＢＴｉ－Ａ２）品系及其亲本籽粒化学成分组成进行的分析比较表明，大豆籽粒中缺失ＳＢＴｉ－Ａ２对其蛋白质含量及天门冬氨酸、甘氨酸和丙氨酸含量有一定影响，表现为缺失ＳＢＴｉ－Ａ２品系显著低于高亲和双亲平均值；而对其他氨基酸和脂肪含量及脂肪酸组成基本没有显著的影响。  相似文献   

Purification and Trypsin Inhibitor Activity of a Sporamin B from Sweet Potato （Ipomoea batatas Lam. 55-2）     

SUN Yan-li  SUN Jun-mao  LI Qing-peng 《中国农业科学(英文版)》2009,8(7):808-820

Sporamin is a soluble protein in sweet potato, and falls into two distinct homology groups, subfamilies A and B. In this research, a sporamin B was purified and its amino acid sequences, trypsin inhibitor activity （Ti activity） were analyzed. This sporamin B was isolated from sweet potato tubers [Ipomoea batatas （L.） Lam cv. 55-2] through extraction of the water-soluble fraction, dialysis, ultrafiltration and ion-exchange chromatography. Homology determined by polyacrylamide gel electrophoresis showed that mainly one bond appeared in gel after being reduced by SDS （sodium dodecyl sulfate）, or by SDS and 2-mercaptoethanol, or in native situation. By comparing the data of the polypeptide mass Matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry with those of the mass of the theoretical amino acid sequences from NCBI protein database, it was revealed that it was Q40091｜Q40091_IPOBA, sweet potato sporamin B - Ipomoea Batatas （sweet potato） （Batate）. The sequence coverage was 70.6%. N-terminal sequence was SETPV （Ser-Glu-Thr-Pro-Val）. There is a linear relationship between trypsin inhibitor activity （Ti activity） and amounts of this sporamin B （3-18 μg mL-1）. The equation of linear regression was y = 2.5809x ＋ 17.049 （r2 = 0.9966）. There was a curvilinear relationship between Ti activity and amounts of this sporamin B （21-150 μg mL-1）. The equation of curvilinear regression is y = 14.417ln（x） ＋ 23.26 （r2 = 0.9924）. The concentration of sporamin B with Ti activity after heating at 40°C may induce part denature of this sporamin B, and there was no statistic difference after heating at 40, 50, 60°C for 20 min. Heat treatment at more than 90°C leads to a dramatic decrease of trypsin inhibitor efficiency. The results suggested that Q40091｜Q40091_IPOBA was the major sporamin B in sweet potato tubers [Ipomoea Batatas （L.） Lam cv. 55-2], which had strong Ti activity, and was stable to both thermal and DTT （DL-dithiothreitol） relatively.  相似文献