Effects of Donor Cells Treated with Egg Extracts on the Development Competence of Cloned Embryos     

GUO Qin-qin  FAN Zong-xing  Hao Hai-sheng  LIU Yan  ZHAO Xue-ming  ZHU Hua-bin  DU Wei-hua 《中国畜牧兽医》2016,43(2):477-486

Japanese Balck cattle fetal fibroblasts (JBCFF) were induced with Xenopus leavis egg extracts and somatic cell nuclear transfer (SCNT) was carried out with the reprogrammed JBCFF as donor cells in order to investigate their effects on SCNT efficiency.Three samples of egg extracts were acquired from different Xenopus laevis.The protein contents and kinds in extracts were evaluated with BCA Protein Quantification Kit and SDS-PAGE.Concentration of Digitonin to permeabilize JBCFF was optimized and assessed with PI staining.Reprogrammed cells treated with egg extract were used as donor in SCNT.Additionally the reconstructed embryos were activated with ionomycin+6-DMAP and A23187+6-DMAP to compare their effects on the development competence.The protein contents of extracts samples were 56.2255,64.6570 and 71.2158 μg/mL,respectively,the each extract had the same composition about 40-55 and 70-100 ku.The optimal concentration of Digitonin was 7 μg/mL and the permeabilization rate was 55.44%.After extracts treatment and continuous culture for 6-7 d,JBCFF formed well-defined colony structures.No significant composition difference was found in rates of fusion (92.83% vs 96.04%),cleavage (89.64% vs 89.78%) and blastocyst formation (24.06% vs 23.12%) of cloned embryos when the colony cells and JBCFF without extracts treatment were used as donor cells (P>0.05).Similarly,the two activation methods had no significant effect on the developmental competence of cloned embryos (cleavage rate 92.16% vs 92.28%,blastocyst rate 23.21% vs 24.18%).Conclusively,Xenopus leavis,egg extracts could induce JBCFF reprogramming to a low differentiated state.However donor cells with reprogramming partially could not improve the development of cloned embryos and its mechanism requires further research.  相似文献   

Effects of Supplemental Complex Water Soluble Vitamin on Production Performance,Blood Biochemical Parameters and Oxidation Resistance of Dairy Cows     

YAO Zhen  WANG Xiao-ming  YANG Zai-bin  LIU Xiao-ming  JIANG Shu-zhen 《中国畜牧兽医》2016,43(4):946-952

The objective of the study was to investigate the effects of supplemental complex water soluble vitamins on production performance, blood biochemical parameters and oxidative resistance of dairy cows in spring and summer.The experiment was designed by 2×2 factorial randomized blocks design.Eighty healthy dairy cows with similar parity and body weight were randomly divided to four groups, with 7 days of adaptation and 70 d experimental phase.The dairy cows in group Ⅲ and Ⅳ were fed a basal diet supplemented with complex water soluble vitamins in spring.The dairy cows in group Ⅱ and Ⅳ were fed a basal diet supplemented with complex water soluble vitamins in summer.The results showed that the ADFI and daily milk yield of dairy cows receiving water soluble vitamin in spring were extremely significantly higher (P<0.01);The ADFI of dairy cows receiving water soluble vitamin was significantly higher in summer (P<0.05) and daily milk yield were extremely significantly higher (P<0.01);The butterfat rate was extremely significantly increased and SCC was extremely significantly decreased in spring and summer (P<0.01);Supplementation of water soluble vitamin was also significantly increased LYM (P<0.05).In summer, T-AOC, T-SOD were extremely significantly increased (P<0.01), and TP, CK, LDH, TP, HDL and ALB were extremely significantly decreased (P<0.01) by water soluble vitamin supplementation than that of control group.In conclusion, complex water soluble vitamins could increase the production performance and non-specific immune function, improve the oxidative resistance and relieved the heat stress of the dairy cows.  相似文献   

假蒟提取物对感染柔嫩艾美耳球虫鸡血液指标的影响     

王定发  周璐丽  李韦  周雄  周汉林 《动物医学进展》2016,(2):57-63

本试验旨在研究假蒟提取物对感染柔嫩艾美耳球虫鸡血液指标的影响。270只1日龄海南文昌公鸡,随机分为6个组,每组3个重复,每重复15只,T1、T2和T3组为对照组(依次分为不感染不给药组、感染不给药组、感染给药组);T4、T5和T6组为假蒟添加组,分别在基础日粮中添加200、400、600mg/kg假蒟提取物粉剂。15日龄时,每组随机抽取30只鸡,除T1组灌服生理盐水外,其他各组鸡均经口接种柔嫩艾美耳球虫卵囊悬液,分别于接种前1d,接种后3、6、9d清晨采血测定其血液指标。结果表明,与感染球虫不给药的T2组相比,假蒟组(T4、T5、T6)可以显著降低感染球虫鸡粪便中球虫卵囊值(P0.05)。假蒟组在感染球虫后第6天,与T1组比,RBC、HGB、HCT、Ca2+、Na+、Cl-、TP、ALB、GLB、TG、CHOL、T4等指标呈下降趋势;但在感染球虫后第9天,TP、GLB含量均显著高于T1组(P0.05);在感染球虫后第6天,假蒟组与对照组相比,ALT、AST浓度水平均显著降低(P0.05)。结果提示,假蒟提取物能提高感染球虫后鸡机体免疫机能,降低肝脏损伤,降低鸡感染球虫后粪便卵囊值,具有一定的抗球虫效果。  相似文献   

灵芝孢子受冷等静高压作用破壁提取机理的探讨     

吴华  张守勤  朱俊洁  王长征 《农机化研究》2005,(6):89-91

按照普通植物种子的情况,推定灵芝孢子的结构有完全充满、部分充满、有大空隙和无种仁4种形式。为此,按照这4种形式分别说明了灵芝孢子受高等静压作用时,孢子壁的破坏方式和灵芝孢子有效成分的溶出方式。  相似文献   

不同水分,温度条件下CO2浓度对大米品质的影响   总被引：5，自引：2，他引：5  

高影  杨建新 《粮食储藏》1997,26(1):3-14

试验选用当年新收获的稻谷加工成的水分为１３．９％、１４．７％和１６．０％的大米，在２３℃和３３℃的恒温室中，将７种不同浓度的ＣＯ２经减压调湿后通入粮堆。定期取样，测定大米的脂肪酸、水溶酸、还原糖、非还原糖、粘度和过氧化物酶活性的变化。试验结果表明：１３．９％水分的大米在２３℃下储藏，各种浓度ＣＯ２对大部分品质指标影响不显著，ＣＯ２的保质作用不明显；１４．７％水分的大米在２３℃储藏，ＣＯ２对个别品质  相似文献   

Study on Effects of the Reaction Conditions on Properties of Poly(aniline-co-o-anisidine)     

TANG Qi  MA Li 《保鲜与加工》2002,(5):46-49

The solubility and processability of polyaniline can be increased through aniline copoly with o-anisidine. Poly(aniline-co-o-anisidine) have good electrochromism .On condition that mole ratio between aniline and anisidine is 3:1,better reaction conditions of synthesizing poly(aniline-co-o-anisidine) with good solubility ,electrochromism and high yield was gotten through researches for effects of monomer ratio,monomer concentration,the amount of oxidant ,reaction times,reaction temperature,acid concentration on properties of poly(aniline-co-o-anisidine).The structure of polymers were characterized by IR spectrum.  相似文献   

乙醇、乙二醇二甲醚与柴油混合燃料排放特性研究   总被引：4，自引：2，他引：4  

郑利  郭和军 《拖拉机与农用运输车》2006,33(2):50-52,55

提出了将乙二醇二甲醚作为乙醇柴油燃料的一种组成成分,利用乙二醇二甲醚好的发火性来改善乙醇柴油燃料差的发火性。试验表明乙二醇二甲醚与乙醇、柴油互溶,具有较好的稳定性。对三种比例混合燃料测试了柴油机在主要工况下的性能与排放特性,得出乙二醇二甲醚加入量以E20为最佳,此时,烟度降低了约80%,NOx略有增加,燃油消耗率略有下降。此外考察了随着十六烷值的变化对混合燃料的排放特性的影响,得出随着十六烷值的增加,NOx排放逐渐增加,对烟度和经济性存在一个最佳值。  相似文献   

基于K均值聚类的厚壁组织区域自动提取   总被引：1，自引：0，他引：1  

王琴  王卫星  张少翃  洪添胜  俞新华 《农机化研究》2006,(10):197-199

桉树是我国南方的速生丰产林,区域提取是定量分析各种组织抑制桉树茎生根原因的重要前提。为此,介绍了基于K均值聚类的在桉树茎切片图像中自适应提取厚壁组织区域的图像处理技术。将桉树茎切片彩色图像转换为CIEL*a*b*彩色空间,用K均值聚类分析算法对描述颜色的a*和b*通道进行聚类分析,提取细胞厚壁,然后填充其中白色的细胞腔,构成完整的厚壁组织区域。实验结果表明,在CIEL*a*b*空间使用该算法可以获得较准确的实验结果。  相似文献   

葡萄果实cDNA文库的构建及鉴定   总被引：1，自引：0，他引：1  

董清华  冯永庆  秦岭  王有年  沈元月 《分子植物育种》2007,5(1):117-120

构建葡萄果实cDNA文库是研究果实发育相关基因表达调控的基础工作.本实验从巨峰葡萄果实中提取了高质量的RNA,利用MMLV反转录酶把总RNA中的mRNA反转录成cDNA第一链,用特异引物进行LD-PCR扩增合成双链cDNA.将分级纯化的带有粘性末端双链cDNA与λTrip Ⅰ Ex2载体连接,重组载体体外包埋成为cDNA原始文库.初步鉴定原始文库的滴度为1.32×106 pfu/mL,文库扩增后滴度达1.45×1010 pfu/mL.从扩增文库中随机挑取250个克隆进行PCR鉴定,结果表明重组率为98.7%,插入片段大小在0.5～2.0 kb之间,因此,本研究成功构建了葡萄果实cDNA文库.  相似文献   

施钾改善木薯农艺性状及淀粉组分     

魏云霞  王娟  李天  刘丽娟  黄洁  郑永清 《热带作物学报》2021,42(1):102-109

以2个木薯品种'SC205' 和'SC124' 为研究对象,设置不施钾(K0,K2O 0 kg/hm2)、低钾(K50,K2O 50 kg/hm2)、中钾(K100,K2O 100kg/hm2)和高钾(K200,K2O 200kg/hm2)4个施钾量处理,测定收获期木薯的农艺性状、鲜薯产量及其构成指标、可溶性糖组分含量...  相似文献