延边黄牛MYH3和MYH8基因多态性及其与生长性状的关联分析     

田佺  王思寒  刘磊  赵祎  柳海星  金花子  李钟淑  张君正  戢爽 《中国畜牧兽医》2021,48(6):2122-2128

本研究旨在探索延边黄牛肌球蛋白重链3（MYH3）和肌球蛋白重链8（MYH8）基因多态性及其与生长性状的关联性,应用DNA直接测序法和高分辨率熔解曲线分型技术对120头24月龄延边黄牛MYH3、MYH8基因的遗传变异进行分析,并结合延边黄牛生长性状数据进行了关联分析。结果显示,在MYH3基因的29602748位点检测到一处T>G突变,包含2个等位基因：T和G,存在3种基因型：TG、TT和GG;在MYH8基因的29406042位点检测到一处C>T突变,包含2个等位基因：C和T,存在3种基因型：CT、CC和TT。关联分析显示,MYH3基因29602748位点T>G突变显著影响24月龄延边黄牛十字部高和腰角宽,表现为GG基因型十字部高显著高于TT基因型（P<0.05）,腰角宽显著高于TT和TG基因型（P<0.05）。MYH8基因29406042位点C>T突变显著影响24月龄延边黄牛体重和胸围,表现为CT和TT基因型体重显著高于CC基因型（P<0.05）;TT基因型胸围显著高于CC基因型（P<0.05）。本研究结果表明,MYH3、MYH8基因多态性与延边黄牛的部分生长性状相关,可作为现代肉牛育种中分子标记辅助选择的候选基因。  相似文献   

基于多源数据融合的盐分遥感反演与季节差异性研究   总被引：7，自引：0，他引：7  

孙亚楠  李仙岳  史海滨  崔佳琪  王维刚  卜鑫宇 《农业机械学报》2020,51(6):169-180

为提高多光谱盐分遥感反演的精度,利用实测高光谱与多光谱进行数据融合,并分析了不同季节盐分遥感的差异性。以河套灌区永济灌域为研究区域,以实测光谱仪测定的土壤高光谱数据和Landsat-8 OLI多光谱数据为基础,通过光谱变换和多元逐步回归方法筛选特征波段和特征光谱指数,构建了春、秋两季土壤盐分多光谱、高光谱反演模型,并利用特征光谱指数的线性回归构建了高-多光谱数据融合反演模型。结果表明:高光谱的反射率总体比多光谱高36. 83%,春季反射率比秋季平均高23. 78%。利用模型中最优变量-特征光谱指数对多光谱模型与高光谱模型进行融合,高-多光谱融合反演模型训练集和验证集R2平均值分别为0. 651和0. 635,RMSE平均值分别为2. 44 g/kg和2. 49 g/kg,精度明显高于对应的多光谱反演模型,其中训练集、验证集的R2平均值分别提高了36. 19%和35. 64%,RMSE平均值分别降低了34. 28%和41. 72%。春季多光谱、高光谱和融合反演模型的精度均高于秋季,其中训练集R2平均值比秋季模型分别提高了6. 03%、6. 05%和4. 40%,验证集R2平均值分别提高了19. 07%、12. 21%和1. 75%。构建的高-多光谱融合模型反演灌域春秋两季平均盐分含量分别为6. 05、5. 97 g/kg,平均相对误差分别为9. 65%和10. 68%,总体上该区域春季土壤主要为重盐化土,秋季土壤主要为中盐化土。  相似文献   

Prediction of pine mistletoe infection using remote sensing imaging: A comparison of the artificial neural network model and logistic regression model     

Ayhan Usta  Murat Yilmaz 《Forest Pathology》2023,53(1):e12783

In this study, the prediction of pine mistletoe distribution in Scots pine ecosystems was explored using remote sensing variables to compare the multilayer perceptron (MLP) artificial neural network (ANN) and logistic regression (LR) model performances. For this purpose, 109 sample plots were distinguished in pure Scots pine forests (natural) in the Eastern Black Sea Region of Turkey. Distinguishing mistletoe-infected stands (69) and uninfected stands (40) was performed with field observations. The variables acquired from Landsat 8 (Level 1) images were used as independent variables for independent-sample t-test, MLP ANN and LR models. Remote sensing variables indicated that mistletoe-infected stands were in drier areas with a lower vegetation-leaf area index. Based on the performance results of both models, the sensitivity (SEN), specificity (SPE), positive predictive value (PPV), negative predictive value (NPV) and accuracy of the MLP ANN model were superior to those of the LR model. The prediction percentages (SEN, SPE, PPV and NPV) of mistletoe-infected stands were better than the prediction percentages of uninfected stands. The prediction accuracies of LR and MLP ANN models were 74.3% and 89.6%, respectively. However, all remote sensing variables were included in the prediction equation of the MLP ANN model, while the thermal infrared 1 (TIRS1) variable was included in the LR model. In the MLP ANN model, the TIRS1 variable also had the highest normalized importance (100%). The area under the curve (AUC) value for identifying the mistletoe-infected stands of Scots pine forests used by the MLP ANN model (0.892 ± 0.034) was higher than in the LR model (0.838 ± 0.039), explaining the more accurate predictions obtained from the MLP ANN model. The MLP ANN model showed much better performance than the LR model. The results of this study are expected to make important contributions to the identification of potential mistletoe-infected areas.  相似文献   

8%胺鲜酯水剂在白菜上应用药效试验     

张柏莲 《福建稻麦科技》2012,30(1):29-30

用8%胺鲜酯水剂在大白菜上进行田间药效试验,试验表明,在大白菜的大田返青期、生长期喷施,能有效地促进大白菜的生长,提高大白菜的产量。对大白菜中的维生素C和可溶性固形物含量等品质指标未发现有不良影响,且对品质有所提升。  相似文献   

犬流感研究进展     

秦海斌  张汇东 《动物医学进展》2010,31(6)

甲型H1N1流感病毒的暴发使得流感病毒的相关研究受到了前所未有的关注,尽管流感病毒的易感种群正在不断扩大已成为事实,但是长久以来的观念仍然认为犬不属于流感病毒的易感动物,直到2004年犬流感病毒H3N8亚型被证实后这种认识才得以改变。论文综述了犬流感病毒H3N8这个犬类新型传染病的病原学和流行病学研究概况,对于了解、研究和预防该病具有借鉴意义。  相似文献   

大豆高赖氨酸基因PM8在烟草中的超量表达及其抗非生物胁迫功能研究     

胡敏  李培青  李杰 《东北农业大学学报》2010,41(6)

应用生物信息学方法在GenBank数据库查找到1条编码大豆LEA3蛋白的高赖氨酸蛋白基因PM8(登录号:Z22872),并利用RT-PCR方法从大豆干种子中克隆得到该基因,序列分析表明,PM8基因与GenBank上的序列存在5个碱基的差异,使编码蛋白的赖氨酸重量比由原来的19.65%增加为19.93%。构建了PM8基因的植物表达载体pEMT-PM8,用农杆菌介导法转化烟草。从转PM8基因的PCR阳性植株中选取5个既抗盐又抗旱的株系进行Real-timePCR检测。结果表明,PM8基因全部超量表达。研究为培育高营养品质且抗逆的转基因作物研究提供了一条新的途径,具有重要的研究价值。  相似文献   

多穗型青贮玉米科多8号制种技术比较     

赵增 《广西农学报》2013,28(3):29-31

科多8号是中国科学院遗传研究所选育的青饲青贮玉米专用品种,深受北方农牧市场的欢迎。但由于制种生产比较效益较低等原因,群众没有制种积极性。文章结合多年来制种工作的经验和近年进行的一些试验研究,从播种期、父母本安排、去雄方法等方面进行深入研究探讨,并提出高效制种模式。  相似文献   

Identification of cDNA for the HSPA2, HSPA5 and HSPA8 orthologs of the heat shock protein 70 family from guinea fowl (Numida meleagris)     

Shigehisa IWAMOTO  Yohei KOIKE  Kazuyoshi HOSOMICHI  Hiromi HARA  Yutaka YOSHIDA  Hiroshi OGAWA  Kei HANZAWA 《Animal Science Journal》2005,76(5):519-524

  相似文献   

Foot-and-mouth disease virus   总被引：11，自引：0，他引：11  

Domingo E  Baranowski E  Escarmís C  Sobrino F 《Comparative immunology, microbiology and infectious diseases》2002,25(5-6):297-308

Foot-and-mouth disease virus (FMDV) is an aphthovirus of the family Picornaviridae and the etiological agent of the economically most important animal disease. As a typical picornavirus, FMD virions are nonenveloped particles of icosahedral symmetry and its genome is a single stranded RNA of about 8500 nucleotides and of positive polarity. FMDV RNA is infectious and it replicates via a complementary, minus strand RNA. FMDV RNA replication is error-prone so that viral populations consist of mutant spectra (quasispecies) rather than a defined genomic sequence. Therefore FMDV in nature is genetically and antigenically diverse. This poses important challenges for the diagnosis, prevention and control of FMD. A deeper understanding of FMDV population complexity and evolution has suggested requirements for a new generation of anti-FMD vaccines. This is relevant to the current debate on the adequacy of non-vaccination versus vaccination policies for the control of FMD.

Résumé

Le virus de la fièvre aphteuse est un aphtovirus de la famille des Picornaviridae et l'agent de la maladie animale la plus importante sur le plan économique. En tant que picornavirus typique, le virus de la fièvre aphteuse est nu, sous forme d'icosaèdre et son génome comprend un acide ribonucléique monobrin avec environ 8500 nucléotides et une polarité positive. L'acide ribonucléique de ce virus est infectieux et il se réplique par l'intermédiaire d'un brin d'ARN moins, complémentaire. La réplication de l'acide nucléique de ce virus conduit à des erreurs, de telle sorte que les populations virales comprennent un ensemble de mutants (quasi espèce) plutôt qu'une séquence génomique bien définie. Par suite, le virus de la fièvre aphteuse est génétiquement et antigéniquement varié. Ceci entraîne des difficultés importantes pour le diagnostic, la prévention et la maîtrise de la fièvre aphteuse. Une connaissance plus approfondie de la complexité et de l'évolution de la population de ce virus a conduit à des besoins pour une nouvelle génération de vaccines aphteux. Ceci est lié au débat actuel sur le choix d'une politique de vaccination ou de non-vaccination dans la lutte contre la fièvre aphteuse.  相似文献   

Effects of pheromone loading, dispenser age, and trap height on pheromone trap catches of the oriental fruit moth in apple orchards     

Orkun B. Kovanci  Coby Schal  F. Walgenbach  George G. Kennedy 《Phytoparasitica》2006,34(3):252-260

The effects of field aging (0–28 days) and pheromone loading rate on the longevity of red rubber septa loaded with the sex pheromone blend of the oriental fruit mothGrapholita molesta (Busck), were evaluated in North Carolina apple orchards in 2002. Separate field tests examined the influence of trap height and pheromone loading rate of rubber septa on trap catches of adultG. molesta males in an abandoned orchard. The loss of the major pheromone component, (Z)-8-dodecenyl acetate (Z8–12:OAc), from red rubber septa over a 4-week period exhibited a relatively constant release rate with 30, 100 and 300 μg pheromone. Trap catch was significantly higher in pheromone traps placed in the upper canopy than in those in the lower canopy. Pheromone traps baited with 100μg lures caught more moths compared with those loaded with 300 μg. There was no apparent relationship between pheromone trap catch and septa age, with trap catch appearing to be primarily a function ofG. molesta population density. http://www.phytoparasitica.org posting May 14, 2006.  相似文献