Iso-nitrogenous and iso-lipidic diets containing 0%, 3%, 6%, 9%, and 12% hydrolyzed porcine mucosa (namely, HPM0, HPM3, HPM6, HPM9, and HPM12) were prepared to evaluate their effects on the growth performance, muscle nutrition composition, texture property, and gene expression related to muscle growth of hybrid groupers (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂). Groupers were fed to apparent satiation at 08:00 and 16:00 every day for a total of 56 days. It was found that the weight gain percentage in the HPM0, HPM3, and HPM6 groups did not differ (P > 0.05). The cooking loss and drip loss of the dorsal muscle in the HPM3 group were lower than those in the HPM6 and HPM9 groups (P < 0.05). The hardness and chewiness of the dorsal muscle in the HPM3 group were higher than those in the HPM0, HPM9, and HPM12 groups (P < 0.05). The gumminess in the HPM3 group was higher than that in the HPM9 and HPM12 groups (P < 0.05). The total essential amino acid content of the dorsal muscle in the HPM12 group was higher than that in the HPM0 group (P < 0.05). The contents of total n-3 polyunsaturated fatty acid and total n-3 highly unsaturated fatty acid, as well as the ratio of n-3/n-6 polyunsaturated fatty acid in the dorsal muscle was higher in the HPM0 group than in all other groups (P < 0.05). The relative expressions of gene myogenic factor 5, myocyte enhancer factor 2c, myocyte enhancer factor 2a, myosin heavy chain, transforming growth factor-beta 1 (TGF-β1), and follistatin (FST) were the highest in the dorsal muscle of the HPM3 group. The results indicated that the growth performance of hybrid grouper fed a diet with 6% HPM and 27% fish meal was as good as that of the HPM0 group. When fish ingested a diet containing 3% HPM, the expression of genes TGF-β1 and FST involved in muscle growth were upregulated, and then the muscle quality related to hardness and chewiness were improved. An appropriate amount of HPM could be better used in grouper feed. 相似文献
The effect of preharvest fruit temperature on the relationships between development of watercore and ethylene evolution, flesh firmness, membrane permeability and sorbitol level in two watercore-susceptible apple (Malus domestica Borkh.) cultivars, ‘Himekami' and ‘Fuji', was investigated at ambient or modified temperatures of 10, 15 or 25°C. Watercore occurrence was completely inhibited at 25°C in both cultivars whereas fruit subjected to 10 or 15°C exhibited significantly higher watercore ratings after two or three weeks of the treatment than those at 25°C and ambient temperatures. Ethylene production of ‘Himekami' apples increased at 25°C and ambient temperature but not at 15°C. Ethylene production was not affected by treatment in ‘Fuji' apples. Flesh firmness of ‘Himekami' tended to maintain a higher level at 15°C than at 25°C, while there was no clear difference among the treatments in ‘Fuji'. The rate of potassium (K) ion elution from the flesh tissues of either cultivar was not affected by fruit temperature treatment. Sorbitol content at 15°C maintained significantly higher level than that at 25°C during the treatment in ‘Himekami' apples although no sorbitol difference was observed among the temperature treatments in ‘Fuji' apples. 相似文献
A 90-day feeding experiment was conducted using flow through system to evaluate the requirement of vitamin E for rohu fry in relation to growth performance and to assess the relationship between the dietary vitamin E levels and carcass composition. Five semi-purified diets supplemented with 0, 50, 100, 150, 200 mg vitamin E/kg dry diet as
--tocopheryl acetate were fed to rohu fry (av. wt.±S.E.=0.58±0.01 g) in triplicate groups. At the end of the experiment, growth and dietary performance were evaluated and vitamin E deposition in the tissue, erythrocyte fragility, thiobarbituric acid reactive substances (TBARs) were analyzed. The average net weight gains were 6.82±0.09, 7.38±0.03, 8.20±0.03, 8.07±0.09 and 8.12±0.08 (g/90 days), respectively, for fish fed diets 0, 50, 100, 150, 200 mg vitamin E/kg. The fish fed diets containing less than 100 mg supplemental vitamin E/kg had significantly (P<0.05) reduced weight gain, feed efficiency and other nutritional indices compared to those fed diets supplemented with vitamin E at 100–200 mg/kg. With the increasing level of vitamin E, both TBARs and erythrocyte fragility values were reduced. Regression analysis of weight gain data using broken-line model indicated a minimum vitamin E requirement of 131.91 mg/kg dry diet. The erythrocyte fragility and TBARs were maximum in fish fed the vitamin E-deficient diet. From the present experiment, it may be concluded that the vitamin E requirement of Labeo rohita fry for optimum growth and other dietary performances is 131.91 mg/kg of dry diet. 相似文献
Selective fishing is the successful capture of target species and size classes in a way that minimises bycatch, minimises damage to flesh, and maximises post-mortem (PM) value. We studied selective harvesting of blue cod Parapercis colias (Pinguipedidae) in central New Zealand, where it is captured mainly by commercial pot-fishing and recreational line-fishing. Potting with paua Haliotis iris (=abalone) guts selectively targeted large blue cod from localities that had six or more fish species and many small blue cod. Video observations of pot entries indicated that blue cod entered and left the pot throughout 30 min sets. Blue cod were observed by video to commence swimming when pot-hauling started. Observations of line-hooked fish indicated a characteristic spinning behaviour while hauling.
Fatigue during harvesting is a major factor in reducing the PM quality and shelf-life of fish muscle. Despite the relatively benign nature of capture via pot, it appears that burst exercise during hauling and a brief flurry of flapping as the pot leaves the water is sufficient to compromise flesh quality. Fish that were fatigued during harvesting had a poorer peri-mortem ‘ATP potential’ than fish that had been tank-rested for a year and then harvested using rested harvesting techniques. Modifying the potting method by providing a reservoir of water reduced peri-mortem fatigue during capture but by an insufficient amount to significantly improve PM flesh quality.
Blue cod flesh is compromised by pot capture, which is widely perceived as a benign harvesting method. In order to maximise value of blue cod, low-stress harvesting methods which take advantage of the behaviour of the fish are required. 相似文献