Serum amyloid A (SAA) mRNA expression in chicken and quails in response to bacterial stress     

《Comparative immunology, microbiology and infectious diseases》2020

Monitoring of acute phase proteins such as serum amyloid A at gene expression level may provide quick information about immune status of the host and its susceptibility towards common infections. Present study was carried out to evaluate and compare the mRNA expression of SAA gene in Rhode Island Red chicken (RIR) and Japanese quails using real time PCR analysis in response to inactivated Salmonella gallinarum culture. The results showed that expression of SAA gene was approximately 17–33 folds higher in case of birds administered with bacterial culture when compared to un-inoculated controls and expression was higher and quicker in case of quails than RIR chicken. The SAA genes from chicken and quail were cloned and upon sequence analysis it was observed that deduced amino acid sequence of SAA from chicken and quails were having approximately seven percent variation which might have significance in function of this protein in these species.  相似文献   

Inlfuence of FSH Treatment on Expression of CDC25A,TSSK3 and P53in Vitro Cultured Sertoli Cells of Calf     

Li Yu-long  Wu Qiong  Zhao Xun-wu  Zeng Yue  Elkanah Adegoke  Zhang Gui-xue 《东北农业大学学报(英文版)》2015,(1)

CDC25A, TSSK3 and P53 expressionsin vitro in cultured sertoli cells after FSH treatment were studied in order to provide some data for further researches of spermatogenesis. Different concentrations of...  相似文献   

小麦面粉中赭曲霉毒素A的CdSe/ZnS荧光量子点免疫探针检测     

齐佳  张文刚  赵静静  史茂敏  李忠宏 《西北农业学报》2015,24(4):56-51

构建一种基于荧光量子点免疫探针的小麦面粉中赭曲霉毒素A(OTA)的检测方法。以EDC为活化剂将CdSe/ZnS半导体荧光量子点与OTA单克隆抗体偶联,制备OTA检测探针并评价其检测性能。结果表明:制备的检测探针在3d保存期内具有良好的稳定性。当OTA质量浓度为0.1~0.9μg/L时,检测探针的荧光强度与OTA质量浓度呈线性关系,其相关系数为0.996 3,检出限为0.017 7μg/L。与国标法对照的结果说明,此方法具有可行性。小麦面粉中的加标回收试验结果显示,OTA的回收率为96.0%~102.7%,相对标准偏差不超过4.1%,说明准确性良好。可见,OTA的CdSe/ZnS荧光量子点免疫探针检测方法操作简单、准确度高、结果稳定,有望应用于小麦面粉中OTA的检测。  相似文献   

早胜牛的采精及细管冻精制作技术要点     

梁永虎 《现代农业科技》2023,(22)

近年来，伴随我国畜牧业蓬勃发展的同时，为了更大限度的提高母畜的受胎率和充分利用优秀种畜的基因，人工授精技术得到了不断完善和发展，并在全国各地广泛推广和应用。本文结合实践经验总结出的一些技术措施，阐述了对早胜牛这一地方优良种质资源进行保种和开发利用的重要意义，同时指出人工授精技术和低温冷冻保存精液技术在早胜牛上的实践，对于进一步开发利用培育优质高产肉牛新品种，促进肉牛产业高质量发展具有非常重要的现实意义。通过早胜牛种公牛采精的全过程以及细管冻精制作要点的详细讲解，低温冷冻保存精液技术，可更经济、可靠地实现家畜品种资源的保护，让优良基因长时间保存使得后续研究需要得到保障。  相似文献   

籼型耐高温三系不育系巨风2A的选育   总被引：2，自引：0，他引：2  

赵开荣  田雨  王学安  张晓玲  方华明  黄代金  张玉祥  贺丽  黄蓉 《湖北农业科学》2016,(2):290-292

以优质常规材料9526为母本,宜陵1B为父本杂交,优选后代与宜陵1A回交而育成的早熟中籼型三系不育系巨风2A,具有耐高温、高异交率、高配合力,所配杂交稻熟色好等优点,应用前景广泛。2014年8月通过了湖北省农作物品种审定委员会的现场鉴定,2015年通过了湖北省审定。  相似文献   

高产、优质多抗夏播糯高梁商梁七号的选育     

孟宪政  袁海波  陈敏菊  陈鑫伟  杜瑞恒  李素英  侯升林 《农业科技通讯》2011,(8):151-152

商粱七号高粱新杂交种是利用824A细胞质雄性不育系作母本，自育恢复系商恢六号作父本，杂交选育而成。经过两年新组合观察试验，两年华北夏播生态区高粱预备区域试验，产量均居第二位，2009—2010年参加两年的华北夏播生态区高粱区域试验，产量居该试验的第二位；该杂交种淀粉含量高达74．20％，支链淀粉占总淀粉的74．19％，是一个比较理想的酿造专用高粱新品种。  相似文献   

Mapping QTL of Fiber Yield and Quality Traits in F2 Populations of Chromosome Segment Substitution Lines from Gossypium hirsutum × Gossypium barbadense     

Guo Lixue  Shi Yuzhen  Li Junwen  Gong Juwu  Liu Aiying  Shang Haihong  Gong Wankui  Chen Tingting  Ge Qun  Sun Jie  Yuan Youlu 《棉花学报》2015,27(6):550-560

In this study, F₂ populations from two chromosome segment substitution lines MBI7455 and MBI7358, were quantified using SSR to evaluate the parents' genotype and detect QTL related to fiber quality plus yield traits of cotton. Results show that the recurrent parent (CCRI45) hosted 96.70% and 95.60% of chromosome segment substitution in MBI7455 with 12 chromosome segments and in MBI7358 with 16 chromosome substitution segments of Gossypium barbadense, respectively. In the F₂ population, the average rate of chromosome substitution of the recurrent parent (CCRI45) was 96.44%, and the average segments of chromosome substitution of Gossypium barbadense was 13.42, with an average segments of homozygous donor chromosome value of 3.90. Analysis showed 19 fiber quality-related QTL with a phenotypic variance of between 2.52%-13.11% and seven yield traits-related QTL with a range of 2.93%-11.40% phenotypic variance, resulting in a total of 26 QTL. The CSSLs could be used to detect QTL for fiber yield and quality traits, which offer an important foundation for the cotton molecular-assisted breeding.  相似文献   

基于Cortex-M4内核的μCOS-Ⅱ移植     

《科技视界》2016,(9)

针对TI公司新推出的基于Cortex-M4内核的TM4C123G高性能低功耗芯片,详细介绍了嵌入式开源实时操作系统μCOS-Ⅱ在芯片上的移植方法。根据移植的需求,首先介绍了芯片的一些基本功能以及相关的软件开发环境,然后结合芯片的固有特性以及μCOS-Ⅱ移植的需求,使用C语言和汇编语言修改了相关的源文件,并详细阐述了修改的原因。  相似文献   

Effect of rifaximin on gut-lung axis in mice infected with influenza A virus     

《Comparative immunology, microbiology and infectious diseases》2021

Gut-lung axis injury is a common finding in patients with respiratory diseases as well as in animal model of influenza virus infection. Influenza virus damages the intestinal microecology while affecting the lungs. Rifaximin, a non-absorbable derivative of rifamycin, is an effective antibiotic that acts by inhibiting bacterial RNA synthesis. This study aimed to determine whether rifaximin-perturbation of the intestinal microbiome leads to protective effects against influenza infection, via the gut-lung axis. Our results showed that influenza virus infection caused inflammation of and damage to the lungs. The expression of tight junction proteins in the lung and colon of H1N1 infected mice decreased significantly, attesting that the barrier structure of the lung and colon was damaged. Due to this perturbation in the gut-lung axis, the intestinal microbiota became imbalanced as Escherichia coli bacteria replicated opportunistically, causing intestinal injury. When influenza infection was treated with rifamixin, qPCR results from the gut showed significant increases in Lactobacillus and Bifidobacterium populations, while Escherichia coli populations markedly decreased. Furthermore, pathology sections and western blotting results illustrated that rifaximin treatment strengthened the physical barriers of the lung-gut axis through increased expression of tight junction protein in the colon and lungs. These results indicated that rifaximin ameliorated lung and intestine injury induced by influenza virus infection. The mechanisms identified were the regulation of gut flora balance and intestinal and lung permeability, which might be related to the regulation of the gut-lung axis. Rifaximin might be useful as a co-treatment drug for the prevention of influenza virus infection.  相似文献   

Comparison of dietary essential amino acid requirements determined from group‐housed versus individually‐housed juvenile bluegill,Lepomis macrochirus     

K. MASAGOUNDER  R.S. HAYWARD  J.D. FIRMAN 《Aquaculture Nutrition》2011,17(2):e559-e571

Two 60‐day experiments were conducted sequentially to determine (i) lysine requirement of juvenile bluegill, Lepomis macrochirus based on the dose–response method, (ii) requirements for other essential amino acids (EAAs) using whole‐body amino acid profile and (iii) whether differences in growth rates of group‐housed versus individually‐housed bluegills lead to different lysine requirement levels because of the presence and absence, respectively, of social hierarchies. Seven, semi‐purified, experimental diets (isonitrogenous, isocaloric) were prepared to contain graded levels of digestible lysine (10–31 g kg⁻¹). Experiment‐1 involved group‐housed bluegills (approximately 27 g, n = 10 fish/chamber, 4 chambers/diet) whereas experiment‐2 involved individually‐housed bluegills (approximately 30 g, n = 1 fish/chamber, 14 chambers/diet). Fish were fed twice daily to apparent satiation. Bluegill growth responses in both experiments generally improved (P < 0.05, anova ) with increasing dietary lysine levels from 10 to 16 g kg⁻¹, and then levelled off with further increase in lysine level (P > 0.05). Optimal dietary lysine level (digestible basis) was estimated to be 15 g kg⁻¹ based on broken‐line regression analyses of relative growth rate and feed conversion ratio with no differences being observed between the two rearing methods. Determined dietary requirement levels for other EAAs ranged from 2.4 g kg⁻¹ (tryptophan) to 15.3 g kg⁻¹ (leucine).  相似文献