Influence of Breed Size,Age, Fecal Quality,and Enteropathogen Shedding on Fecal Calprotectin and Immunoglobulin A Concentrations in Puppies During the Weaning Period          下载免费PDF全文

A. Grellet  R.M. Heilmann  B. Polack  A. Feugier  C. Boucraut‐Baralon  D. Grandjean  N. Grützner  J. S. Suchodolski  J.M. Steiner  S. Chastant‐Maillard 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(4):1056-1064

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Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen     

Hiroshi BANNAI  Manabu NEMOTO  Koji TSUJIMURA  Takashi YAMANAKA  Ken MAEDA  Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):309-311

To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.  相似文献   

小麦异代换系的选育及其应用研究进展     

李萍  王黎明  李本  王洪刚 《安徽农业科学》2007,35(13):3824-3825

综述了小麦异代换系的主要选育方法,分析了小麦异代换系的应用现状及存在问题,提出了进一步的研究方向.  相似文献   

Quantification of stress sensitive markers in single fecal samples do not accurately predict excretion of these in the pig   总被引：1，自引：0，他引：1  

Carlsson HE  Lyberg K  Royo F  Hau J 《Research in veterinary science》2007,82(3):423-428

All feces produced during 24 h were collected from five pigs and cortisol and immunoreactive cortisol metabolites (CICM), and IgA were quantified. Within pigs, the concentrations of CICM and IgA varied extensively between random samples obtained from a single fecal dropping, and deviated in most cases significantly from the true concentration measured in total fecal output (CV 6.7-130%). The CICM and IgA contents varied considerably (CV 8.1-114%) within and between individual fecal droppings from the same pig compared to the total fecal excretion. In conclusion, single random samples could not be used to reliably quantify the total fecal concentration or excretion of CICM or IgA in pigs. Analyses of all feces collected during shorter periods than 24 h did not provide an accurate estimate of the daily excretion of CICM. Thus, the concentration of stress sensitive molecules in random single fecal samples as an indicator of animal welfare should be interpreted with prudence.  相似文献   

Increased abundance of patatins,lipoxygenase and miraculins in a thaxtomin A-habituated potato Russet Burbank somaclone with enhanced resistance to common scab     

Iauhenia Isayenka  Juan Duque-Yate  Marie-Claire Goulet  Dominique Michaud  Carole Beaulieu  Nathalie Beaudoin 《Plant pathology》2023,72(1):100-111

Potato common scab caused by the actinobacterium Streptomyces scabiei is characterized by the formation of corky lesions on tubers that reduce their marketability. Management of common scab is very complex and often ineffective under various environmental conditions. Using potato varieties that are more resistant to common scab remains one of the most efficient strategies to control this disease. However, very little is known about the factors associated with resistance to common scab. Somaclone RB9 regenerated from thaxtomin A-habituated potato Russet Burbank calli produced tubers more resistant to common scab than the original variety. Comparison of the RB9 tuber proteome with that of Russet Burbank using label-free quantitative proteomic analysis revealed changes in the accumulation of defence-related proteins from the patatin and lipoxygenase (LOX) families, which are involved in the metabolism of lipids, and of two miraculins of the Kunitz-type protease inhibitors family. The implication of LOX during common scab infection was studied using synchronized minitubers developed from leaf-bud cuttings. S. scabiei infection stimulated the accumulation of LOX in both Russet Burbank and RB9 minitubers, but this accumulation was intensified in RB9 minitubers. Infection also increased LOX activity in Russet Burbank and RB9 minitubers. However, LOX activity measured in noninfected RB9 minitubers was similar to that of infected Russet Burbank minitubers, indicating endogenous activation of LOX activity in RB9 minitubers. We discuss how increased LOX abundance and activity in the somaclone RB9 may contribute to improving tuber defence against common scab.  相似文献   

三棱栎种子特性与其致濒关系初步分析   总被引：3，自引：0，他引：3  

周元 《种子》2004,23(1):13-15

三棱栎是云南特有种,国家二级保护植物,主要分布在澜沧、沧源、孟连、西盟、勐腊等县.研究表明,其萌发温度在20～35℃范围内,萌发率在5.3%～9.1%之间.4个不同分布地和8种温度条件下萌发率差异不显著.本文认为三棱栎濒危的主要原因是:(1)三棱栎果实结实率低;(2)果实的特殊形状,不利于传播;(3)幼苗缺乏竞争力.针对三棱栎的濒危现状和原因,提出了三棱栎的一些保护措施.  相似文献   

THE REIMPROVEMENT FOR IMPULSE OF G. I. TAYLOR THEORY OF PLASTIC DEFORMATION IN IMPACT OF CYL1NDICAL PROJECTILES     

Liu Xiangchen Li Zeqiang 《保鲜与加工》1991,(5):109-115

In this paper the reimprovement for G. I. Taylor theory of plastic deformation in impact of cylindical projectiles is done. The impulse in the reimproved theory is G. I. Taylor' s 1/2, and 3/4 of G. I. Taylor' s improved theory. The analytical solution of the reimproved theory shows that it gives better agreement with exprimental results than those of the original Taylor' s theory and, the improved theory.  相似文献   

A.A. Marcov Forecast Method of the System Ration in Estate Business Study     

YAN Wei  QIAN Yu-yu 《保鲜与加工》2004,(2):110-115

A. A. Markov Rolling Forecast Method is presented in this paper according to the dynamic characteristics of the estate business system. Test results reveal that the system quantitative forecast could be realized on the basis of previous samples, which provide a direction of the rational management in estate business. Concerning sample molding analysis and Markov Rolling Forecast Method, this paper gives a corresponding conclusion of analytic research quantitatively in order to demonstrate the formation of factors working in estate business activities and its systematic logistic connotation. Markov Rolling Forecast Method can be applied not only to short-term prediction but long term one as well, provided when the market is rather stable with no significant change during a regular period of time and when the rolling times of the state transition matrix are fairly enough.  相似文献   

黎榆A繁殖母本有效穗和结实率与产量的关系   总被引：5，自引：0，他引：5  

黄大军  谭亚玲  李炎渊  顾晓明 《种子》2003,(1):14-15

繁殖田母本有效穗和结实率是影响产量的主要因素。通过对10个不同父母本栽插行比的分析结果表明：黎榆A繁殖产量以父母本栽插行比为2：18最高，达292．09kg/667m^2。增加有效穗对于增产的效应要比增加结实率大；有效穗与产量，结实率与产量的偏相关系数均呈正相关，且有效穗与产量的相关达显著水平，多元回归方程为：y-155.6283 4.8660x 0.2342x2.  相似文献   

基于UEGO的柴油机EGR系统空燃比MAP的制取     

刘瑞祥  高希彦 《农业装备与车辆工程》2005,(12):19-22

在柴油机稳态工况下,通过试验测定了不同空燃比时,NOx、HC、CO、烟度和油耗的变化.通过对测试数据的分析,确定了最佳空燃比MAp.为基于UEGO的柴油机EGR系统的闭环控制奠定了基础.  相似文献