转基因抗草甘膦油菜内源特异参照基因BnACCg8和外源E9 3''终止子的检测研究   总被引：4，自引：0，他引：4  

潘良文  胡永强  张舒亚 《中国油料作物学报》2003,25(2):74-77

对转基因抗草甘膦油菜内源特异参照基因BnACCg8和外源基因E9 3’终止子进行了普通PCR和。TaqMan实时荧光PCR检测，建立了相应的检测方法。  相似文献   

应用电镜与PCR技术检测王官溪蜜柚黄龙病病原   总被引：8，自引：1，他引：7  

田亚南  李韬 《植物病理学报》2000,30(1):76-81

 福建省产区发生王官溪蜜柚叶片斑驳、果实变小及全株矮化的病树是柑桔黄龙病引起的。采用电镜技术与PCR技术研究,证明其病原BLO在形态、大小与构造上和柑桔黄龙病病原(BLO)相同,其病原DNA、PCR扩增及Southern Blot分子杂交的结果,证明它与柑桔黄龙病病原PCR的产物具有很高的同源性。本病具有传染性,能通过病梢嫁接传染给王官溪蜜柚的健苗。  相似文献   

气调包装技术在食用菌保鲜中的研究进展     

金玮玲  高虹  范秀芝  程薇  史德芳 《保鲜与加工》2017,17(6):126-131

简单介绍了气调包装保鲜技术的发展和特点,综述了气调包装保鲜机制、保鲜效果以及在新鲜食用菌保鲜中的应用,旨在为我国研究并推广食用菌气调包装保鲜技术提供理论基础和技术参考。  相似文献   

Immunomodulatory activities of non-prolamin proteins in wheat germ and gluten     

《Journal of Cereal Science》2017

Albumin (Alb), globulin (Glo), glutelin (Gll) and glutenin (Gln) were separately extracted from wheat germ and wheat gluten. Amino acisd composition, molecular weight distribution, solubility, in vitro digestibility, and immunomodulatory activities were all analyzed. Gll and Gln have similar molecular weight distributions, which differed from those of Alb and Glo. Alb showed the highest solubility at various pH values (except pH 4.0), whereas Glo showed the highest in vitro digestibility. Glo and Gll have the highest proportion of essential to total amino acids, while Alb and Gll have the highest protein digestibility-corrected amino acid scores. Gll had the strongest immunomodulatory effects in terms of stimulation of RAW 264.7 cells to produce IL-6, TNF-α, and IL-10, and good stimulatory effects on splenocyte proliferation, production of IL-2, phagocytosis, and secretion of nitric oxide in RAW 264.7 cells. Gll can be considered a good protein source for use in health foods.  相似文献   

Assessing fitness parameters of hybrids between weed beets and transgenic sugar beets          下载免费PDF全文

Henri Darmency 《Plant Breeding》2017,136(6):969-976

Herbicide resistance is a desired trait for sugar beet (Beta vulgaris L.) production because it is a low‐competitor crop that requires careful weed management. However, gene flow to weed beet (also B. vulgaris) could jeopardize the weed control strategy by causing the emergence of resistant weed beets; it could also lead to further adaptation of weed beet as a troublesome weed for other crops by selecting more competitive plants. To evaluate the hazard that such a selection process represents, apart the herbicide resistance, we investigated the morphology and reproduction of progeny of weed beets having inherited a herbicide resistance gene. First‐generation resistant weed beet exhibited traits likely counter‐selected. But such crop traits rapidly disappeared with backcrossing to weedy relatives: no biologically relevant difference was noted between resistant and susceptible near‐isogenic siblings in the various experiments. In the absence of resistance selection, our data indicate low chances for weed beet to evolve more competitive forms than present weed beet populations. However, they also suggest that there is no fitness cost limiting transgenes spread.  相似文献   

Evaluation of stress- and immune-response biomarkers in Atlantic salmon, Salmo salar L., fed different levels of genetically modified maize (Bt maize), compared with its near-isogenic parental line and a commercial suprex maize     

Sagstad A  Sanden M  Haugland Ø  Hansen AC  Olsvik PA  Hemre GI 《Journal of fish diseases》2007,30(4):201-212

  相似文献   

Challenges in developing fish-based ecological assessment methods for large floodplain rivers     

J. J. DE LEEUW  A. D. BUIJSE  G. HAIDVOGL  M. LAPINSKA  R. NOBLE  R. REPECKA  & T. VIRBICKAS  W. WI&#;NIEWOLSKI  C. WOLTER 《Fisheries Management and Ecology》2007,14(6):483-494

Abstract  Large European floodplain rivers have a great diversity in habitats and fish fauna, but tend to be heavily modified. The complexity of these river systems and their multiple human impacts pose considerable challenges for assessment of their ecological status. This paper discusses: (1) the application of historical information on fish fauna and habitat availability to describe reference conditions; (2) responses of fish assemblages to human disturbance by comparing various rivers and river segments with different impacts and/or time series within rivers; (3) the role of floodplain water bodies in ecological assessment; and (4) monitoring of large rivers using different gears and sampling designs for main channels and floodplain habitats. The challenge for the future is to standardise and calibrate sampling methods and data to enhance the potential for ecological assessment of large rivers.  相似文献   

谷子种子高质量总RNA提取方法的优化     

王金荣  许冰霞  尹美强  温银元 《核农学报》2020,34(4):729-734

为得到高质量的谷子种子RNA,本研究以5个不同谷子品种为材料,对RNAiso Plus法、改良RNAiso Plus法、CTAB法提取的RNA进行质量和纯度的比较。结果表明,改良RNAiso Plus法提取的总RNA完整性、纯度、浓度均优于其他2种方法提取的总RNA,5个不同谷子品种和不同萌发时期的谷子均可用改良RNAiso Plus法提取出高质量、完整性好的RNA,所得RNA产物可用于构建cDNA文库和转录组测序等后续试验。本研究为禾谷类植物种子的总RNA提取提供了一定的参考,也为谷子种子后续的分子生物学试验奠定了基础。  相似文献   

川西高山树线群落交错带地被物及土壤的水文效应   总被引：2，自引：0，他引：2  

刘洋  张健  杨万勤  吴福忠  黄旭  闫帮国  文维全  胡开波 《林业科学》2011,47(3)

采用样带法,以川西米亚罗林区鹧鸪山高山树线群落交错带为研究对象,分析交错带地被物和土壤的持水特性及其变化规律。结果表明:地被物最大持水量为33.55～140.03t·hm-2,暗针叶林>林线>树线>密灌丛>草甸>疏灌丛,暗针叶林枯落物最大持水量显著高于疏密灌丛和草甸;林线地被物拦蓄降水的作用最明显;暗针叶林以苔藓层和枯落物层具有最大有效拦蓄效果,林线和树线以苔藓层具有最大有效拦蓄效果,疏密灌丛以枯落物层具有最大有效拦蓄效果,高山草甸草本层、苔藓层、枯落物层有效拦蓄效果相当;0～30cm土壤饱和蓄水量都在2000t·hm-2以上,暗针叶林>林线>疏灌丛>密灌丛≈草甸>树线;各群落0～30cm土层非毛管持水量为575.89～801.46t·hm-2,表现为暗针叶林>疏灌丛>树线>密灌丛>林线>草甸。  相似文献   

VA菌根的研究进展及其应用前景   总被引：14，自引：0，他引：14  

李志真  谢一青 《江西农业大学学报》2002,24(4):448-453

综述近年来国内外有关VA菌根的研究进展 ,其中包括VA菌根的生理生态学特性、VA菌根与植物病害、VA菌根与有益土壤生物之间的关系等 ,并探讨了VA菌根在农林业生产上的应用方法和策略及其前景  相似文献