Peripheral blood leukocytes transcriptomic signature highlights the altered metabolic pathways by heat stress in zebu cattle     

Vamsikrishna Kolli  R.C. Upadhyay  Dheer Singh 《Research in veterinary science》2014

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Aortic expression of HSP22, TNF-α and eNOS in rats with hyperlipidemia and effects of atorvastatin     

FANG Hai-yang  CHEN Qi  XIANG Jian  WU Yan-qing  CHENG Xiao-shu 《园艺学报》2014,30(10):1873-1878

AIM:To establish a rat hyperlipidemia model for studying the aortic expression of heat shock protein 22 (HSP22), tumor necrosis factor alpha (TNF-α) and endothelial nitric oxide synthase (eNOS) and the effect of atorvastatin intervention. METHODS:Hyperlipidemia model was established in SD rats. Afterwards, the rats were divided into normal control group, high fat group and high fat+atorvastatin intervention group. The expression of HSP22 and TNF-α in the rat aortas was detected by immunohistochemical assay and the expression of eNOS was assessed by Western blotting. RESULTS:No detectable expression of HSP22 and TNF-α in the normal control group was observed. However, the expression of HSP22 and TNF-α was positive in the high fat group and the atorvastatin intervention group. The mean densities of HSP22 and TNF-α positive particles were significant lower in the atorvastatin intervention group as compared with high fat group (both P＜0.05). The expression of eNOS protein in the high fat group and atorvastatin intervention group was significantly lower than that in normal control group (P<0.01). However, no marked difference of eNOS protein expression between high fat group and atorvastatins intervention group was observed. CONCLUSION: The expression of HSP22 and TNF-α in the rat aortas is increased in the hyperlipidemia rat model. This effect can be restored by atorvastatin treatment. The expression of eNOS in the rat aortas is decreased in the hyperlipidemia rat model, but this tendency could not be attenuated by atorvastatin.  相似文献   

辣椒苗期耐热性及其生理生化特性研究     

姜燕  黄新根  杨寅桂  周庆红  郑咪  肖旭峰 《长江蔬菜》2014,(12):47-50

对6个耐热性不同的辣椒品种进行高温处理,计算热害指数,并研究辣椒幼苗叶片中超氧化物歧化酶、过氧化物酶的活性变化,以及丙二醛、脯氨酸的含量变化。结果表明,辛香8号和辛香24号耐热性较强;随着胁迫温度的升高,辣椒叶片中SOD的活性均表现为先上升后下降;POD活性与品种耐热性无明显相关性;MDA含量和脯氨酸含量与胁迫温度呈正相关。SOD活性、PRO和MDA含量变化可以作为辣椒耐热性鉴定的生理指标,而POD活性则不适合用于鉴定辣椒的耐热性。  相似文献   

Heat stress: A major contributor to poor animal welfare associated with long-haul live export voyages     

Malcolm P. Caulfield  Heather Cambridge  Susan F. Foster  Paul D. McGreevy 《Veterinary journal (London, England : 1997)》2014,199(2):223-228

Recent investigations by the Australian Department of Agriculture, Fisheries and Forestry into high mortalities on live export voyages from Australia to the Middle East during the Northern hemisphere summer suggest that animal welfare may be compromised by heat stress. The live export industry has generated a computer model that aims to assess the risk of heat stress and to contain mortality levels on live export ships below certain arbitrary limits. Although the model must be complied with under Australian law, it is not currently available for independent scientific scrutiny, and there is concern that model and the mandated space allowances are inadequate. This review appraises the relevant literature on heat stress in sheep and cattle, including laboratory studies aimed at mimicking the ambient temperatures and humidity levels likely to be encountered on live export voyages. Animal welfare is likely to be very poor as a result of heat stress in some shipments.  相似文献   

Amino acid digestibility of heat damaged distillers dried grains with solubles fed to pigs     

Ferdinando Nielsen Almeida  John Kyaw Htoo  John Thomson  Hans Henrik Stein 《畜牧与生物技术杂志(英文版)》2014,(1):88-97

The primary objective of this experiment was to determine the effects of heat treatment on the standardized ileal digestibility(SID) of amino acids(AA) in corn distillers dried grains with solubles(DDGS) fed to growing pigs. The second objective was to develop regression equations that may be used to predict the concentration of SID AA in corn DDGS. A source of corn DDGS was divided into 4 batches that were either not autoclaved or autoclaved at130°C for 10, 20, or 30 min. Four diets containing DDGS from each of the 4 batches were formulated with DDGS being the only source of AA and CP in the diets. A N-free diet also was formulated and used to determine the basal endogenous losses of CP and AA. Ten growing pigs(initial BW: 53.5 ± 3.9 kg) were surgically equipped with a T-cannula in the distal ileum and allotted to a replicated 5 × 4 Youden square design with 5 diets and 4 periods in each square. The SID of CP decreased linearly(P 0.05) from 77.9% in non-autoclaved DDGS to 72.1, 66.1, and 68.5% in the DDGS samples that were autoclaved for 10, 20, or 30 min, respectively. The SID of lysine was quadratically reduced(P 0.05) from 66.8% in the non-autoclaved DDGS to 54.9, 55.3, and 51.9% in the DDGS autoclaved for 10, 20, or30 min, respectively. The concentrations of SID Arginine, Histidine, Leucine, Lysine, Methionine, Phenylalanine, or Threonine may be best predicted by equations that include the concentration of acid detergent insoluble N in the model(r2= 0.76, 0.68, 0.67, 0.84, 0.76, 0.73, or 0.54, respectively). The concentrations of SID Isoleucine and Valine were predicted(r2= 0.58 and 0.54, respectively) by the Lysine:CP ratio, whereas the concentration of SID Tryptophan was predicted(r2= 0.70) by the analyzed concentration of Tryptophan in DDGS. In conclusion, the SID of AA is decreased as a result of heat damage and the concentration of SID AA in heat-damaged DDGS may be predicted by regression equations developed in this experiment.  相似文献   

The effect of gas to liquid ratio on carbon dioxide removal and heat loss across a forced ventilated trickling filter     

《Aquacultural Engineering》2020

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中药对热应激蛋鸡外周血中主要离子和酶类浓度的影响(英文)     

高桂生  史秋梅  沈萍  刘欢  邢超  张雪莲  高光平  张艳英 《农业科学与技术》2014,15(12):2171-2174

[目的]研究中药对蛋鸡在热应激条件下血液生化指标的影响,以期为临床应用提供理论和实际的依据。[方法]将100只88日龄的伊莎褐蛋鸡随机分成五组,分别设为中药方一组,中药方二组,VC对照组,热应激空白组和常温对照组。将中药方一组,中药方二组,VC对照组和热应激空白组蛋鸡置于人工制造的热应激环境下,常温对照组蛋鸡置于正常的温度条件下,分4批不同时间宰杀抽血取样,观察各组每批蛋鸡组间血液生化指标情况。[结果 ]高温时蛋鸡血钠(Na),血磷(P),血钾(K)和血氯(Cl)含量分别降低,而丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)及肌酸激酶(CK)均上升,使用中药后,各生化指标逐渐趋于正常。[结论 ]抗热应激中药可以从各种离子和酶类不同代谢途径来缓解热应激对蛋鸡血液生理生化指标所造成的不利影响。  相似文献   

控制瑞香试管苗玻璃化的研究   总被引：44，自引：0，他引：44  

周菊华  林证明 《园艺学报》1990,17(3):229-232

本文报道了瑞香试管玻璃苗发生规律的研究结果:1.外植体取材部位显著影响玻璃苗发生频率,顶芽外植体的玻璃苗发生频率远低于茎段、基部茎段的远低于中部茎段,2.蔗糖浓度、琼脂浓度均不影响玻璃苗发生频率,3.在MS基本培养基中减去NH_4NO_3可以显著降低玻璃苗发生频率,4.BA NAA比KT IBA容易引发玻璃苗的发生,5.降低培养温度不能减少玻璃苗的发生,6.对瑞香愈伤组织进行40℃ 0.5h的热击处理,可以完全消除玻璃苗的发生。  相似文献   

循环泵运行故障分析及排除   总被引：3，自引：3，他引：0  

肖庆荣  葛秀成  游玉萍  于海 《排灌机械》2004,22(1):47-48

循环供水系统具有节水、节能的优点，在污水处理、冷却水循环、供热系统等领域中应用十分广泛。介绍了曲阜市水利学院的供热系统的运行情况，通过分析其循环泵的故障成因及处理办法，说明了循环泵运行中值得注意的问题。  相似文献   

Preparation of recombinant PTD-HSP27 and verification of its ability to penetrate the cell membrane of human lens epithelial cells and rabbit cornea     

LIU Lian  YU Rong-jie  DAI Yun  ZENG Zhi-xing  GUO Xiao-ling  JI Qing-shan  ZHONG Jing-xiang 《园艺学报》2015,31(1):135-140

AIM: To construct the prokaryotic expression system containing protein transduction domain (PTD) with heat shock protein 27 (HSP27) in order to prepare and purify the recombinant protein, and to verify whether the recombinant protein PTD-HSP27 has the ability to penetrate the human lens epithelial cell (HLEC) membrane and the rabbit cornea. METHODS: The plasmid pKYB-PTD-HSPB1-6His was constructed by the technique of overlap extension PCR. The plasmid was transformed and PTD-HSP27 was purified through nickel affinity chromatography column and identified by Western blotting. PTD-HSP27-6His was labeled with the fluorescein isothiocyanate (FITC). The penetrating ability of PTD-HSP27 into HLECs and rabbit cornea was tested. RESULTS: The recombinant PTD-HSP27 plasmid was successfully cloned and effectively expressed. The correctness of the recombinant protein PTD-HSP27 was demonstrated. Fluorescence microscopic examination showed that PTD-HSP27-FITC was internalized by HLECs. Fluorescent labeled PTD-HSP27 was then observed in the rabbit aqueous humor. CONCLUSION: The recombined gene PTD-HSPB1 was constructed by overlap extension PCR technique and the PTD-HSP27 fusion protein was prepared and purified by nickel affinity chromatography column. Using the technique of PTD-fusion protein, HSP27 was transduced into HLECs and passed through the cornea.  相似文献