中药对热应激蛋鸡外周血中主要离子和酶类浓度的影响(英文)     

高桂生  史秋梅  沈萍  刘欢  邢超  张雪莲  高光平  张艳英 《农业科学与技术》2014,15(12):2171-2174

[目的]研究中药对蛋鸡在热应激条件下血液生化指标的影响,以期为临床应用提供理论和实际的依据。[方法]将100只88日龄的伊莎褐蛋鸡随机分成五组,分别设为中药方一组,中药方二组,VC对照组,热应激空白组和常温对照组。将中药方一组,中药方二组,VC对照组和热应激空白组蛋鸡置于人工制造的热应激环境下,常温对照组蛋鸡置于正常的温度条件下,分4批不同时间宰杀抽血取样,观察各组每批蛋鸡组间血液生化指标情况。[结果 ]高温时蛋鸡血钠(Na),血磷(P),血钾(K)和血氯(Cl)含量分别降低,而丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)及肌酸激酶(CK)均上升,使用中药后,各生化指标逐渐趋于正常。[结论 ]抗热应激中药可以从各种离子和酶类不同代谢途径来缓解热应激对蛋鸡血液生理生化指标所造成的不利影响。  相似文献   

控制瑞香试管苗玻璃化的研究   总被引：44，自引：0，他引：44  

周菊华  林证明 《园艺学报》1990,17(3):229-232

本文报道了瑞香试管玻璃苗发生规律的研究结果:1.外植体取材部位显著影响玻璃苗发生频率,顶芽外植体的玻璃苗发生频率远低于茎段、基部茎段的远低于中部茎段,2.蔗糖浓度、琼脂浓度均不影响玻璃苗发生频率,3.在MS基本培养基中减去NH_4NO_3可以显著降低玻璃苗发生频率,4.BA NAA比KT IBA容易引发玻璃苗的发生,5.降低培养温度不能减少玻璃苗的发生,6.对瑞香愈伤组织进行40℃ 0.5h的热击处理,可以完全消除玻璃苗的发生。  相似文献   

循环泵运行故障分析及排除   总被引：3，自引：3，他引：0  

肖庆荣  葛秀成  游玉萍  于海 《排灌机械》2004,22(1):47-48

循环供水系统具有节水、节能的优点，在污水处理、冷却水循环、供热系统等领域中应用十分广泛。介绍了曲阜市水利学院的供热系统的运行情况，通过分析其循环泵的故障成因及处理办法，说明了循环泵运行中值得注意的问题。  相似文献   

Preparation of recombinant PTD-HSP27 and verification of its ability to penetrate the cell membrane of human lens epithelial cells and rabbit cornea     

LIU Lian  YU Rong-jie  DAI Yun  ZENG Zhi-xing  GUO Xiao-ling  JI Qing-shan  ZHONG Jing-xiang 《园艺学报》2015,31(1):135-140

AIM: To construct the prokaryotic expression system containing protein transduction domain (PTD) with heat shock protein 27 (HSP27) in order to prepare and purify the recombinant protein, and to verify whether the recombinant protein PTD-HSP27 has the ability to penetrate the human lens epithelial cell (HLEC) membrane and the rabbit cornea. METHODS: The plasmid pKYB-PTD-HSPB1-6His was constructed by the technique of overlap extension PCR. The plasmid was transformed and PTD-HSP27 was purified through nickel affinity chromatography column and identified by Western blotting. PTD-HSP27-6His was labeled with the fluorescein isothiocyanate (FITC). The penetrating ability of PTD-HSP27 into HLECs and rabbit cornea was tested. RESULTS: The recombinant PTD-HSP27 plasmid was successfully cloned and effectively expressed. The correctness of the recombinant protein PTD-HSP27 was demonstrated. Fluorescence microscopic examination showed that PTD-HSP27-FITC was internalized by HLECs. Fluorescent labeled PTD-HSP27 was then observed in the rabbit aqueous humor. CONCLUSION: The recombined gene PTD-HSPB1 was constructed by overlap extension PCR technique and the PTD-HSP27 fusion protein was prepared and purified by nickel affinity chromatography column. Using the technique of PTD-fusion protein, HSP27 was transduced into HLECs and passed through the cornea.  相似文献   

基于转录组测序的苦瓜热激蛋白相关基因分析     

杜文丽  陈中钐  许端祥  徐同伟  高山  温庆放 《分子植物育种》2020,(10):3141-3149

为获得苦瓜叶片高温胁迫状态下表达的相关基因,本研究以高温胁迫下苦瓜叶片为实验材料,采用Illumina HiSeq 2500高通量测序技术对正常温度(26℃)和高温胁迫(38℃)下苦瓜叶片转录测序后进行组装,再利用KEGG、Pfam、Swiss-Prot和NR数据库平台对热激蛋白的相关功能基因筛选,共获得24个热激蛋白相关基因,包括得20个热激蛋白基因、2个位于叶绿体中的小热激蛋白以及2个热激转录因子。聚类分析研究表明,涉及到热激蛋白相关的20个Unigene广泛分布于3大类中,其中c42567和c43094这两个亲缘关系最近;两个小热激蛋白c42963和c12133的亲缘关系次之;c35683和c3040两个热激转录因子亲缘关系最远。通过本次研究中获取的24个热激蛋白相关Unigene信息,有助于深入研究苦瓜耐高温胁迫机理等提供数据信息。  相似文献   

热激处理对甘蓝幼苗叶片细胞膜系统热稳定性的影响   总被引：2，自引：0，他引：2  

陈碧华  罗庆熙  王广印  李贞霞  孙涌栋 《华北农学报》2007,22(5):60-62

采用二因素(热激温度、热激时间)二次回归正交旋转组合设计,对耐热性不同的2个甘蓝品种的种子进行了热激处理,然后在苗期测定了甘蓝幼叶中细胞膜的电解质渗漏量。结果表明:热激处理后不耐热品种叶片中细胞膜透性的增幅大于耐热品种,热激温度、热激时间二因素与细胞膜热稳定性之间有显著的回归关系,并且回归分析出最优热激处理组合为42℃2.3 h。  相似文献   

小麦耐热性基因的染色体定位和遗传效应分析   总被引：1，自引：0，他引：1  

陈希勇赵爱菊  李亚军刘玉萍 《华北农学报》2007,22(B10):1-5

以中国春-HOPE染色体代换系为试验材料，利用细胞膜热稳定性和大田生产条件下高温胁迫两种方法，对与耐热性有关的基因进行了染色体定位。结果表明，无论是利用膜热稳定性方法，还是利用大田高温胁迫环境进行耐热性评价的方法，HOPE的2A，3A，2B，3B和4B染色体代换到中国春相应染色体后，均显著提高了中国春的耐热性，证明这些染色体含有与耐热性有关的基因。在大田高温胁迫环境条件下，HOPE的3D，4A，5A和5B染色体也表现与耐热性有关。利用单染色体代换系和不同耐热性代换系杂交组合，对耐热性的基因效应分析结果显示，HOPE2A染色体上的耐热性基因表现为显性效应，3A，2B，3A和4B染色体上的耐热性基因表现为加性效应；3B与4B，3A与2A，3B与2B以及这些染色体上的耐热性基因与2A染色体上的耐热性基因之间均可能存在互作效应。  相似文献   

高温胁迫对小麦幼苗几个生理生化指标的影响     

郭洪雪宋希云  燕增文裴玉贺王海娟  刘兰浩 《华北农学报》2007,22(B08):71-74

采用人工气候室控温,在大气相对湿度40%的条件下,以22℃为对照,在32,36,40℃的高温胁迫条件下,对烟农19二叶期小麦叶片内叶绿素含量、超氧化物岐化酶总活力、丙二醛含量和可溶性糖含量的变化规律进行了研究。结果表明：随着胁迫处理时间的延长,叶片内叶绿素含量迅速降低,且随胁迫温度的升高,叶绿素降解率增大;超氧化物歧化酶（SOD）活性及膜脂过氧化产物（MDA）含量呈现先上升后下降趋势;可溶性糖含量呈上升趋势,且随胁迫温度的升高,可溶性糖含量升高幅度呈上升趋势。这些变化可能是由于高温胁迫造成细胞内膜系统损害所致。  相似文献   

Effect of high-pressure hot-water washing treatment on fruit quality, insects, and disease in apples and pears: Part I. System description and the effect on fruit quality of ‘d’Anjou’ pears     

Jinhe Bai  Eugene A. Mielke  Paul M. Chen  Robert A. Spotts  Maryna Serdani  James D. Hansen  Lisa G. Neven 《Postharvest Biology and Technology》2006,40(3):207-215

A manually operated high-pressure hot-water washing system consisting of a boiler, hot-water mixing tank, contact loop, heat exchanger, spray mixing tank, high-pressure hot-water washing manifold, low-pressure fresh water rinse manifold, and pressure pump was constructed and installed in a packingline. The system developed 20–50 °C washing water at pressures up to 980 kPa. ‘d’Anjou’ pears (Pyrus communis L.), shortly after harvest, and after storage for 3 and 4 months in regular air (RA) or for 4, 7 and 8 months in controlled atmosphere (CA) at −1 °C were washed through the packingline with different wetting agents (0.1% Silwet, 0.01 and 0.1% Defoamer, and water), water pressures (regular and high-pressure (210–980 kPa)), water temperatures (control (tap water, 4–22 °C), 40 °C, and 50 °C), and brushes (soft and firm), respectively. The effect of the washing conditions on fruit quality was investigated after 1 month of storage at −1 °C to simulate shipping condition, and then again after 1 week at 20 °C to simulate marketing condition. Hot-water caused severe heat scald. When nozzle temperature was 50 °C, the incidence of heat scald increased to over 50% for the fruit stored in RA for 3 months. Combined with hot-water, 540 kPa high-pressure washing increased the incidence of friction discoloration. There were lower incidences of friction discoloration and heat scald for fruit stored in CA for 7 months, in comparison to that in RA for 3 months. However, those fruit did not ripen properly as indicated by a high extractable juice content. Fruit washed at harvest had minor incidences of friction discoloration regardless of different brushes, water pressures, and wetting agents. Fruit washed after storages in either 4 months RA or 4 or 8 months CA suffered a high incidence of friction discoloration including scuffing symptoms and pressure marking. The firm brushes caused a higher incidence of friction discoloration mainly because of scuffing symptoms. However, no differences were found between different water pressures and wetting agents with respect to friction discoloration. Fruit stored for 4 months RA suffered 26–28% friction discoloration in comparison to 16–18% in CA stored fruit with firm brush washing. Extended CA storage increased friction discoloration even with soft brush washing. The results suggest that a washing system with high-pressure spray, <30 °C warm water, wetting agent Defoamer and rotating soft brushes were significantly effective in removing surface pests and decay control without causing internal or external damage of fruit.  相似文献   

热处理条件对番茄采后冷害的影响   总被引：11，自引：2，他引：11  

李丽萍  韩涛 《农业工程学报》1996,12(4):189-193

研究了绿熟番茄采后在３３℃和３８℃加热２，５，８ｄ及２±１℃下冷藏后的果实品质、冷害程度和生理变化。结果表明，适宜的热处理可降低果实呼吸强度、细胞膜电解质渗出率和丙二醛含量，增加游离脯氨酸含量，减轻果实冷害，且对果实硬度和可溶性固形物含量无显著影响。其中以处理温度时间组合３３℃、５ｄ和３８℃、２ｄ的效果最好；３３℃、２ｄ的处理温度较低、时间较短，因而效果不明显；８ｄ的处理时间过长，使细胞受到伤害，丙二醛含量和细胞膜电解质渗出率增加，果实明显变软，出现异味。番茄果实的冷害指数（ＣＩＩ）与细胞膜电解质渗出率呈正相关，相关系数ｒ＝０．９７４４。  相似文献