亚致死浓度Bt对棉铃虫齿唇姬蜂寄生几率及生长发育的影响   总被引：4，自引：1，他引：4  

孙长贵  张青文  徐静  王因霞  周明 《中国生物防治》2003,19(3):106-110

在亚致死浓度下，研究了Bt对棉铃虫齿唇姬蜂寄生几率和生长发育的影响。结果表明：随着Bt浓度的增加，明显抑制了棉铃虫幼虫的生长，从而显著延长齿唇姬蜂适合寄生的时间；近似蜂幼虫和蜂成虫的体重随着Bt浓度的增加而下降，蜂成虫寿命随着Bt亚致死浓度的增加而缩短，而齿唇姬蜂卵和幼虫期随着Bt浓度的增加而延长；Bt对齿唇姬蜂茧期和茧重也有一定的影响，但没有一致的趋势。  相似文献   

棉铃虫滞育的遗传方式   总被引：6，自引：0，他引：6  

吴孔明  郭予元 《植物保护学报》1998,25(1):6-10

采自北京和湖北省武穴市的棉铃虫（ＨｅｌｉｃｏｖｅｒｐａａｒｍｉｇｅｒａＨｕｂｎｅｒ）在２４℃,１２：１２（Ｌ：Ｄ）光周期下的滞育充分别为８０．３２％和５３．００％对北京群的滞育性和武穴种群的滞育不敏感性分别进行５代和１４代选育,北京种群（Ｂ）的滞育反应无明显变化,而武穴种群（Ｗ）则稳定在２０％～３０％之间,表明武穴种群存在较大的遗传异质性,对二者的杂交试验显示,显性系数ＤＷＢ和ＤＢＷ分别为－０．２  相似文献   

苏云金杆菌预处理对棉铃虫乙酰胆碱酯酶的影响   总被引：8，自引：1，他引：7  

谭维嘉  梁革梅  郭予元 《植物保护学报》1998,25(1):65-71

用苏云金杆菌（Ｂｔ）预处理２４ｈ后,棉铃虫幼体内乙酰胆碱酯酶对特异性酶抑制剂的亲和力发生了变化,在抗性品系中,幼虫的乙酰胆碱酯酶的酶动力学值和抑制中浓度均低于未处理对照幼虫,双分子速率常数则高于未处理对照幼虫,单位时间内抑制作用加快,差异显著,说明Ｂｔ预处理可能改变或激活子抗性幼虫乙酰胆碱酯的活化中心,因而增加了其幼虫乙酰胆碱酯酶与抑制剂的亲和力,由此推论,乙酰胆碱酯酶的改变是Ｂｔ预处理棉铃虫幼由  相似文献   

几株高效苏云金杆菌菌株对玉米螟、棉铃虫、粘虫和黄地老虎的毒力测定   总被引：6，自引：2，他引：6  

冯书亮  付韵琴  范秀华  王容燕  邢建民  胡明峻 《中国生物防治》1995,(1)

本文以玉米螟、棉铃虫、粘虫和黄地老虎为供试昆虫，对筛选出的７株高效苏云金杆菌菌株的孢晶制剂进行了毒力测定。以ＬＣ５０为衡量各菌株的毒效指标，属血清型Ｈ６的“Ｇ０２”菌株对上述４种供试昆虫的毒力最高，属血清型Ｈ３ａ３ｂ的“８０－１”菌株对棉铃虫和粘虫的毒力也高于“ＨＤ－１”菌株。这一研究结果为今后生产高效的苏云金杆菌制剂及生产菌株的轮换提供了后备菌株  相似文献   

棉铃虫幼虫对氨基酸、糖类和棉花次生物质的取食选择性     

张继红  王琛柱 《植物保护》2001,27(1):3-5

研究测定了棉铃虫对 30种氨基酸、10种糖类和 4种棉花次生物质的取食选择性。结果显示初孵幼虫对0.1g/ml的L-苏氨酸、DL-2-氨基-n-丁酸、L-亮氨酸、L-缬氨酸、DL-瓜氨酸、L-丙氨酸、D-海藻糖、D-果糖以及棉花次生物质儿茶精、棉酚、槲皮素表现出较强的趋性反应 ,但将L-苏氨酸、L-亮氨酸、D-瓜氨酸、L-缬氨酸、L-苯丙氨酸以0.009g/ml的浓度、槲皮素和10种糖物质以0.025g/ml的浓度分别掺入人工饲料中,5日龄幼虫仅对D-海藻糖表现出高的取食选择性  相似文献   

新疆喀什棉铃虫基础生化及氰戊菊酯对表皮的穿透性研究     

罗万春  慕卫  谭维嘉  徐遥 《植物保护学报》2001,28(2):169-172

从新疆喀什采集棉铃虫（Helicouerpa armigera Hubner)建立实验室种群，测定其基础生化指标及药剂对表皮的穿透性，同时与山东夏津的抗性棉铃虫的同类数据进行了比较。结果表明，两地棉铃虫体内乙酰胆碱酯酶活性无显著差异。而羟酸酯酶、酸性磷酸酯酶及谷胱甘肽-S-转移酶的活性有显著差异。对酶动力学数据Km和Vmax的分析表明，两地间棉铃虫体内酶活性的差异主要是量的差异，酶的本质并无区别，氰戊菊酯对新疆喀什棉铃虫表皮穿透性大于山东夏津棉铃虫。  相似文献   

Effects of a primitive cotton accession on feeding ofspodoptera littoralis andhelicoverpa armigera and on oviposition ofBemisia tabaci     

A. Navon  Venezia Melamed-Madjar  M. Zur 《Phytoparasitica》1991,19(2):143-147

A primitive cotton(Gossypium hirsutum L.) accession, V-64, from the Caribbean Islands was bioassayed for antibiosis against pests of this crop in comparison with the commercial strains Acala SJ-2 and Pima S-5. V-64 reduced weight gain and leaf consumption ofSpodoptera littoralis andHelicoverpa armigera, and significantly reduced oviposition ofBemisia tabaci. The leaf pigment glands in V-64 were larger and more abundant than in the commercial strains, and the feeding ofH. armigera on these glands was avoided, indicating that the glands probably constituted the major resistance character in V-64. Contribution from the Agricultural Research Organization. No. 3187-E, 1991 series.  相似文献   

转Bt基因抗虫棉棉铃虫防治技术研究   总被引：9，自引：5，他引：4  

王留明  隋玉君 《山东农业科学》1998,(3):13-16

１９９５～１９９７年就转Ｂｔ基因抗虫棉对棉铃虫的抗性及棉铃虫的防治技术进行了研究。结果表明，抗虫棉对棉铃虫具有很强的内在抗性，对初孵幼虫致死率达９０％以上，对幼虫的生长发育影响严重，其毒力随幼虫龄期的增大而降低。在棉铃虫大发生年份，对二、三代棉铃虫不进行药剂防治则显著减产。初步提出抗虫棉的棉铃虫防治策略为：二代以保棉株生长点为主，三代要重点防治以保蕾铃。其防治指标是：二代棉铃虫百株累计卵量２００～３００粒，三代百株累计卵量８０～１２０粒。  相似文献   

Functional expression of Helicoverpa armigera CYP9A12 and CYP9A14 in Saccharomyces cerevisiae     

Yihua Yang  Song Chen 《Pesticide biochemistry and physiology》2008,92(2):101-105

Elevated oxidative detoxification is a major mechanism responsible for pyrethroid resistance in Helicoverpa armigera from Asia. Constitutive overexpression of CYP9A12 and CYP9A14 was associated with pyrethroid resistance in the YGF strain of H. armigera. CYP9A12 and CYP9A14 were functionally expressed in the W(R) strain of yeast (Saccharomyces cerevisiae) transformed with a plasmid shuttle vector pYES2. The cell lysates prepared from yeast transformed with CYP9A12 and CYP9A14, respectively, exhibited considerable O-demethylation activities against two model substrates p-nitroanisole (0.59 and 0.42 nmol p-nitrophenol min⁻¹ mg protein⁻¹) and methoxyresorufin (2.98 and 5.41 pmol resorufin min⁻¹ mg protein⁻¹), and clearance activity against the pyrethroid esfenvalerate (8.18 and 4.29 pmol esfenvalerate min⁻¹ mg protein⁻¹). These results provide important evidence on the role of CYP9A12 and CYP9A14 in conferring pyrethroid resistance in H. armigera, and also demonstrate that the yeast expression system can provide necessary redox environment for insect P450s to metabolize xenobiotics.  相似文献   

Construction of the recombinant baculovirus AcMNPV with cathepsin B-like proteinase and its insecticidal activity against Helicoverpa armigera     

Hong-Lian Shao  Du-Juan Dong  Jin-Xin Wang 《Pesticide biochemistry and physiology》2008,91(3):141-146

The Helicoverpa armigera cathepsin B-like proteinase (HCB) has been shown to have a wide spectrum of substrates. It has been involved in the degradation of yolk protein during embryonic development and also in the decomposition of the adult fat body. To study the possibility of using HCB to improve the insecticidal activity of bioinsecticides, it was inserted into the pFASTBACDUAL-green fluorescent protein (GFP) donor plasmid under the strong polyhedrin promoter, and the polyhedrin gene was retained behind the HCB gene so that the virus could orally infect the host and survive in the natural environment. After the recombinant plasmid transfected the Sf21 cells, the recombinant baculovirus Autographa californica multiple nucleocapsid nucleopolyhedrovirus (AcMNPV)-GFP-HCB-Polh⁺ was produced. A 37-kDa recombinant procathepsin B was expressed in AcMNPV-GFP-HCB-Polh⁺-infected Sf21 cells and processed into 29/25-kDa mature forms. Gelatin zymography revealed that the proteolytic activities of the recombinant HCB and other proteases were activated or enhanced by HCB in AcMNPV-GFP-HCB-Polh⁺-infected larvae. Green fluorescence was observed earlier and was more intense in AcMNPV-GFP-HCB-Polh⁺-infected larvae than in HCB-free AcMNPV-GFP-Polh⁺-infected ones that were infected at the same time; this indicated that the AcMNPV-GFP-HCB-Polh⁺ virus spreaded faster in larvae than the AcMNPV-GFP-Polh⁺ virus. A bioassay revealed that infection with the AcMNPV-GFP-HCB-Polh⁺ virus shortened the median survival time of H. armigera larvae by 12 h as compared with those infected with the AcMNPV-GFP-Polh⁺ or the wild-type AcMNPV virus. These results suggest that HCB may possibly play a role in the recombinant virus in improving the rate of killing larvae.  相似文献