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高效阴离子交换色谱法测定毛头鬼伞多糖中的单糖组成 总被引:2,自引:0,他引:2
本文采用高效阴离子交换色谱—脉冲安培检测器(HAPEC-PAD),建立了一种测定多糖中单糖比例的方法。以NaOH为淋洗液、CarboPac^TM A20预处理柱,CarboPac^TMPA20分离柱,金工作电极,Ag/AgCl参比电极,8种自然界中常见单糖标准品做混合标样,探索方法可行性。在淋洗液浓度为2.5mmol/L时,各种单糖组分得到有效分离,其线性和重现性均良好并在此基础上测定了毛头鬼伞多糖中单糖比例;与传统的方法相比,此方法具有前处理简单、灵敏度高、节省时间和试剂等优点。 相似文献
24.
Effects of land ownership and landscape-level factors on rare-species richness in natural areas of southern Ontario,Canada 总被引:5,自引:0,他引:5
Lovett-Doust J. Biernacki M. Page R. Chan M. Natgunarajah R. Timis G. 《Landscape Ecology》2003,18(6):621-633
Surprisingly few studies have considered the extent to which the nature of the ownership of land is associated with differences
in biodiversity. We analysed ownership and other landscape-level effects on rare-species richness for both globally- and regionally-rare
biota (including birds, herpetofauna, butterflies, mammals, and plants) in 289 designated natural areas (NAs) in southern
Ontario, Canada. Information about each NA −including area, number of plant communities, ownership status and details of species
diversity were collected from published sources. Length of perimeter of NA, relative isolation, and an estimate of fragmentation
were measured using image analysis and GIS techniques. NAs were in general relatively small, with mean area of 158 ha (median
85 ha, range from 0.9 to 1278 ha) for private NAs; public NAs had mean area of 132 ha (median 16 ha, range from 0.1 to 1481
ha). Mean number of plant communities was 4.6 (median 4, range 1- 13) at private NAs and 3.8 (median 3, range 1-16) at public
NAs. Our results show that, of several landscape-level factors, area had the greatest effects on rare-species richness and
other biotic indices. Effects of area were followed by effects of plant community diversity, however this was itself significantly
affected by area and the extent of perimeter of the NA. Both these factors were followed by effects of ownership of the NA
and by effects of isolation of the NA (represented by minimum distance to nearest NA and by number of NAs in 10 km radius).
Other landscape- level factors did not appear to have overall significant effects. Variation in area accounted for 0.1% to
29% of variation in number of rare species, with lower values for globally-rare, than for regionally-rare taxa. For all biotic
groups, public ownership of NAs was associated with significantly greater rare-species richness compared to private ownership,
even after other factors such as area were controlled. For all globally-rare biota except butterflies, area of NA had greater
effects on rare-species richness than did ownership. Richness of regionally- rare birds was more affected by plant community
diversity than by area of NA. Number of recorded plant communities accounted from 2.1% of variation in number of globally-rare
plant species to as high as 31% of variation in regionally-rare butterflies. The diversity of plant communities was itself
influenced by total site area (accounting for 45% of variation), extent of elongation of the NA, and both external- and interior-
edge perimeters. Public NAs had greatest numbers of rare biota and so should be a significant focus for conservation programs.
Smaller, privately-owned patches of natural area dominate (by number and area) in this densely populated region and their
significance should not be overlooked.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
25.
红肉脐橙果肉中主要色素的定性及色素含量的变化 总被引:23,自引:3,他引:23
运用薄层层析法及二极管阵列检测器与高效液相色谱联用的方法, 确定红肉脐橙果肉中含有番茄红素和β- 胡萝卜素两种主要色素, 它们在液相色谱图中占总峰面积(计溶剂峰) 的73. 57 %~77. 78 % , 番茄红素占55. 36 %~58. 49 % , β- 胡萝卜素占18. 21 %~19. 93 %。利用反相高效液相色谱仪以及番茄红素和β- 胡萝卜素标样制作标准曲线, 测定果实发育及室温贮存过程中色素的变化动态, 结果表明, 该脐橙果肉中的番茄红素在成熟期含量最高, 达109. 67μg·g-1DM, β- 胡萝卜素此时也达到最高值(15. 52μg·g-1DM) ; 番茄红素的含量在采收后基本呈下降趋势(但采后30 d 有显著升高) , 说明果肉中存在类胡萝卜素采后合成的现象; 贮存4 个月后, 番茄红素含量急剧降低至最高时的1/ 10。 相似文献
26.
早熟、优质、耐贮梨新品种新梨7号选育研究 总被引:7,自引:0,他引:7
以库尔勒香梨为母本,早酥梨为父本,有性杂交选育成新梨7号品种。该品种同时兼备优质、早熟与耐贮藏性;该品种遗传了父本的早熟性,比父本更早熟15~20d,盛花期~果实可食期约80~95d,属于早熟品种;且遗传了母本品质性状的质地酥脆、耐贮、清香、阳面红晕,比母本果个大,果心小。果柄木质化,丰产抗风。新梨7号果实贮藏期的可溶性固形物、果实重量、果实硬度和果实前期腐烂率的变化与库尔勒香梨基本相似,但耐热能力高于母本。凡适宜香梨、早酥、苹果梨和巴梨种植的地区,都适宜该品种的种植。 相似文献
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分蘖洋葱最突出的特性是分蘖性强,生长快,分蘖的多少直接影响分蘖洋葱的产量。试验表明:12-20℃条件最适宜其生长,良好的土壤营养和适宜的环境有利于分蘖洋葱的分蘖生长,获得高产、优质商品。 相似文献
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