opaque-2玉米近等基因系的构建与赖氨酸含量快速检测   总被引：2，自引：0，他引：2  

陈岩  宋丽雅  周志强  张德贵  颜娜  张世煌  李新海  郝转芳  翁建峰  白丽  李明顺 《作物学报》2013,39(9):1530-1537

我国高赖氨酸玉米种质资源狭窄,opaque-2(o2)突变基因能大幅提高玉米赖氨酸含量,通过分子标记辅助选择构建o2玉米近等基因系并检测其赖氨酸含量具有重要意义。其中,要解决的两个关键问题是如何准确地将不同供体的o2突变基因导入受体系和如何快速地检测导入系的赖氨酸含量。本研究利用O2基因内紧密连锁的SSR共显性标记引物phi057检测玉米供体和受体自交系的多态性,利用其特异性和共显性构建o2近等基因系;参考已有研究,改进染料结合(DBL)法,测定18组构建成功的o2近等基因系的赖氨酸含量。结果表明,不仅在不同供体(CA339和山东2548)之间存在多态性,而且在不同受体系间也存在多态性,利用phi057能够成功地将不同供体的o2突变基因导入受体系,构建o2近等基因系;改进的DBL法分析表明,不同受体系赖氨酸含量变化较大,不同背景的受体系导入o2突变基因后赖氨酸含量增加的幅度差异也较大;普通玉米自交系间赖氨酸含量为0.223%~0.368%,构建成功的不同o2近等基因系间,赖氨酸含量为0.373%~0.527%,与受体亲本相比,赖氨酸增加幅度最低为13%,最高为74%。分析表明,phi057能准确筛选导入o2突变基因的受体系,结合改进的DBL法能快速地选择赖氨酸含量高的玉米。  相似文献   

耐低氮烟草基因型的筛选及氮效率分析     

钟思荣  陈仁霄  陶瑶  龚丝雨  何宽信  张启明  张世川  刘齐元 《作物学报》2017,43(7):993-1002

筛选耐低氮及氮高效的烟草基因型是提高氮素利用效率,减少氮污染的一种有效途径。本文采用营养液培养方法,于苗期以低氮(0.5 mmol L–1)和正常氮(5.0 mmol L–1)处理74个不同基因型烟草,通过指标统计、因子分析、聚类分析来确定评价指标及筛选出耐低氮基因型,并结合氮效率综合值分析表明,在低氮和正常氮条件下,不同基因型烟草的根系体积、根系生物量、茎叶氮累积量、地上部生物量变异系数均较大,分别为0.37~0.68和0.38~0.64。低氮和正常氮的主成分基本相似,在不同供氮条件下茎叶氮累积量、地上部生物量均起主要作用。筛选出15个耐低氮基因型,占供试材料的20.3%,其中8个属于低氮高效正常氮低效型,占耐低氮基因型的53.3%,6个属于低氮低效正常氮低效型,占40.0%,1个属于低氮高效正常氮高效型,占0.7%;筛选出8个低氮敏感基因型,其中6个属于低氮低效正常氮高效型,占75.0%,2个属于低氮低效正常氮低效型,占25.0%。初步确定14P9为耐低氮、氮高效基因型,中烟100和K394为低氮敏感、氮低效基因型。  相似文献   

正齿行星轮分插机构的动力学分析   总被引：1，自引：4，他引：1  

俞高红  陈建能  赵凤芹  赵匀 《农业机械学报》2005,36(4):51-55

采用推导的刚体复合运动微分方程和动力学方程组逐次求解的方法对正齿行星轮分插机构进行动力学分析,建立其动力学模型,得到在插秧过程中链条、太阳轮轴心、中间轮轴心、行星轮轴心、太阳轮与中间轮啮合点和中间轮与行星轮啮合点受力与行星架转角的关系,为进一步对该机构强度分析和动力学优化提供理论基础。  相似文献   

外源物质处理对高温胁迫下苹果果实抗氧化能力的影响   总被引：7，自引：0，他引：7  

张建光  李英丽  邸葆  张健强  刘玉芳 《华北农学报》2004,19(3):55-58

高温胁迫下,果实表皮组织抗氧化代谢变化的总趋势是O2-和MDA含量升高,当胁迫在一定范围内,SOD和POD活性上升;超过一定限度,SOD和POD活性下降。外源自由基产生剂能够增加内源O2-的含量,同时极显著提高SOD的活性。试验结果表明,在45℃温度胁迫下,果实表皮组织O2-含量增加14.52％~61.70％;SOD活性提高1.07~1.66倍。不同种类外源活性氧产生剂对内源O2-含量的影响效能有所不同。碱性二亚硫酸钠可直接产生O2-,所以诱导内源O2-合成的效果更大一些。不同种类外源物质均可显著或极显著提高果实表皮组织SOD和POD活性,提高果实的抗热性。  相似文献   

不同覆盖方式对植烟土壤温度和水分及烤烟品质的影响   总被引：8，自引：4，他引：8  

李正风  张晓海  刘勇  吴伯志  夏玉珍  杨清辉 《中国农学通报》2006,22(11):224-224

不同覆盖方式的比较试验结果表明,秸秆与地膜双覆盖能够协调烤烟生长全过程的土壤温度,并且地温日变化幅度小,蓄水保墒作用显著。秸秆单覆盖对土壤性状改良与秸秆和地膜双覆盖相似,烟叶内在化学成分较协调,品质较高,但由于在烤烟生长前期不能提高土壤温度,使得烟叶生长速度较慢,产量下降;地膜单覆盖对土壤性状改良不及秸秆单覆盖和秸秆与地膜双覆盖,秸秆和地膜双覆盖使0~20cm土层含水量平均比露地栽培提高16.82%,比秸秆单覆盖提高6.45%,比地膜单覆盖提高5.43%,而且烟叶成分较协调,产量与产值也较高。  相似文献   

Production protocol development for greenhouse cut Linaria, Lupinus, and Papaver flowers     

John M. Dole  Lane Greer 《Scientia Horticulturae》2009

Linaria maroccana Hook. f. Ann., ‘Lace Violet’, Lupinus hartwegii ssp. cruikshankii Lindl. ‘Sunrise’ and Papaver nudicaule L. ‘Meadow Pastels’ seeds were directly sown into 105 cell plug trays and received either ambient light or supplemental high intensity discharge (HID) lighting. For each species, a 2 × 3 × 3 factorial was used with two light intensities during propagation, three transplant stages, and three night temperatures. Seedlings were transplanted at the appearance of 2–3, 5–6, or 8–9 true leaves. Transplanted Linaria and Papaver seedlings were placed at 5/11, 10/16, or 15/21 ± 1 °C night/day temperatures and Lupinus seedlings were placed at 15/24, 18/25, or 20/26 ± 2 °C night/day temperatures. For this study, the optimum production temperature for Linaria was 10/16 °C as the cut stems produced at 15/21 °C were unmarketable and production time was excessively long at 5/11 °C. At 10/16 °C, Linaria seedlings should be transplanted at the 2–3 leaf stage to maximize stem number, stem length and profitability. For Lupinus the optimum temperature was 15/24 °C due to long stems and high profitability per plant. Lupinus seedlings should be transplanted at the 2–3 leaf stage when grown at 15/24 °C to obtain the longest and thickest stems; however, $/m² week was higher for plants transplanted at the 8–9 leaf stage due to less time in finishing production space. For Papaver, the 15/21 °C temperature was optimal as that temperature produced the longest stems in the shortest duration, resulting in the highest $/m² week. At 15/21 °C Papaver plants should be transplanted at the 2–3 leaf stage. Supplemental HID lighting had no effect on any of the species.  相似文献   

云南陇川县水田甘蔗高产高糖种植技术     

邓美峰  方陶伟 《中国甜菜》2009,(4):52-53

介绍了云南陇川县水田新植和宿根甘蔗的高产高糖种植技术。  相似文献   

国家甜菜区试品种在甘肃河西走廊丰产与稳定性及抗病性评价     

骆爱基  赵玮 《中国甜菜》2009,(1):24-26

用品种稳定性参数及高稳系数（HSC）,对2004～2005年参加国家甜菜区试中的5个品种在甘肃武威市黄羊镇和酒泉地区的试验结果进行了综合分析,结果表明：BTS356的丰产、稳产性好．两年平均根产量和产糖量分别较对照陇糖2号增产40．62％和39．94％,差异达极显著水平,含糖率18．73％;对黄化病、白粉病、立枯病的抗性优于对照,具有较高的应用价值,适宜在甘肃省河西走廊生态条件下推广种植。  相似文献   

Seed yield and water use efficiency of canola (Brassica napus L.) as affected by high temperature stress and supplemental irrigation     

Abolfazl Faraji  Nasser Latifi  Amir Hossain Shirani Rad 《Agricultural Water Management》2009,96(1):132-140

The effects of high temperature stress and supplemental irrigation on seed yield and water use efficiency (WUE) of canola (Brassica napus L.) were studied in a field experiment conducted for 2 years. The experiment was a randomized complete block design arranged in split plot, conducted at Agricultural Research Station of Gonbad, Iran. It was arranged in two conditions, i.e. supplemental irrigation and rainfed. Two cultivars of canola (Hyola401 and RGS003) as subplots were grown at five sowing dates as main plots. The sowing dates were 9 November, 6 December, 5 January, 4 February and 6 March in 2005-2006 and 6 November, 6 December, 5 January, 4 February and 6 March in 2006-2007, to have a wide range of environmental conditions around flowering and seed filling periods, and to coincide reproductive stages of the crop with high temperature stress. Seed yield was improved due to field management practices, such as supplemental irrigation and optimum sowing date. Supplemental irrigation was an efficient practice to mitigate water stress, and to increase aboveground dry matter and seed yield. There was a strongly negative relationship between seed yield and air temperature during reproductive stages. Delay in sowing led to more rapid developmental of canola, decreased aboveground dry matter, leaf area index (LAI), harvest index (HI), WUE, and seed yield. Achieving a high aboveground dry matter was an essential prerequisite for high reproductive growth and a high seed yield. Greater seed yield and WUE at first sowing date were associated with greater LAI and aboveground dry matter, and lower temperatures during reproductive stages. The results support the view that WUE can be used as an indirect selection criterion for seed yield in genotypic selection.  相似文献   

农业综合开发与高效农业结合的思考     

王朝霞 《农机具之友》2009,(9):27-28

落实科学发展观,提高农业综合生产能力,促进农民增收,农业增效,是农业综合开发工作的出发点和落脚点。以泗阳县为例,就如何做好农业综合开发与高效农业的结合。提出了农业综合开发工作更好地服务、支持现代高效农业的发展思路和措施。  相似文献