电位控制血红蛋白(Hb)组装制备无媒介体过氧化氢生物传感器     

谢轶  袁若  柴雅琴 《安徽农业科学》2009,37(33):16721-16724

通过电位控制的方法将血红蛋白Hb固定在由L 半胱氨酸自组装修饰的金电极上制得过氧化氢传感器Hb/L cys/Au,并通过电化学方法（CV）、原子力显微镜（AFM）等手段对电极的修饰过程进行了表征。试验显示,该传感器对H2O2催化还原性能优良、灵敏度高、稳定性好且非常易于制做。电极在（pH值5.5）0.1 mol/L PBS中对H2O2催化还原响应良好,线性可分为2段,低浓度段为8.21×10^－8～4.83×10^－6mol/L,高浓度段为4.83×10^－6～8.22×10^－3 mol/L,检测限为8.24×10^－9mol/L。  相似文献   

Azide ion as a quinone methide scavenger in the horseradish peroxidasecatalyzed polymerization of sinapyl alcohol     

Yuki Tobimatsu  Toshiyuki Takano  Hiroshi Kamitakahara  Fumiaki Nakatsubo 《Journal of Wood Science》2008,54(1):87-89

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基于产品风味和色泽的青芥辣工艺的优化     

丁绍东  丁占生  刘绘  范柳萍  王国华 《安徽农业科学》2016,44(20)

[目的]研究开发风味好、色泽纯的青芥辣产品,丰富我国调味品市场。[方法]以辣根为原料,研究了发制温度、发制时间、p H对青芥辣产品异硫氰酸烯丙酯和色泽的影响,在单因素试验的基础下,采用3因素3水平正交试验优化青芥辣产品的加工工艺。[结果]青芥辣的较优生产工艺为发制温度30℃、发制时间2 h、p H 3.5,该工艺条件下制得的青芥辣产品具有较好的色泽与风味。[结论]研究可为辣根的深加工利用提供参考。  相似文献   

酶标胶固素免疫测定的建立     

刘胜江  杜念兴  徐为燕 《南京农业大学学报》1986,(3)

本文报道了一种新的酶免疫技术——酶标胶固素免疫测定(EKIA)。用猪血清白蛋白(SSA)包被微量滴定板后,加入适当稀释度的兔抗SSA血清、马血清(补体)和酶标胶固素(HRP-K)各50μl,于37℃下反应2小时。洗涤后加入底物显色,即可用分光光度计测定反应结果。本法操作简单、快速、灵敏度高,能检出兔抗SSA的IgG_(0.52)μg/ml;重复性较好,变异系数为7.46-16.67％之间;特异性强,能为相应抗原和EDTA所阻断;具有通用性,适用于人、猪、豚鼠、免、大鼠和鸡抗体的免疫检测。  相似文献   

Substituent effects of 3,5-disubstituted p-coumaryl alcohols on their oxidation using horseradish peroxidase–H2O2 as the oxidant     

Takayuki Kobayashi  Hiroyasu Taguchi  Mikiji Shigematsu  Mitsuhiko Tanahashi 《Journal of Wood Science》2005,51(6):607-614

The consumption rates of three monolignols (p-coumaryl, coniferyl, and sinapyl alcohols) and eight analogues using horseradish peroxidase (HRP)–H₂O₂ as an oxidant were measured and compared with the anodic peak potentials thereof measured with cyclic voltammetry. 3-Monosubstituted p-coumaryl alcohols, i.e., 3-methoxy-, 3-ethoxy-, 3-n-propoxy-, and 3-n-butoxy-p-coumaryl alcohols, had faster reaction rates than p-coumaryl alcohol. This is most probably due to the electron-donating effect of alkoxyl groups. However, the reaction rates gradually decreased with an increase in the molecular weight of the alkoxyl groups. Furthermore, t-butoxyl group, which is a very bulky substituent, caused an extreme reduction in the reaction rate, even though its electron-donating effect was almost the same as that of other alkoxyl groups. The reaction rates of 3,5-disubstituted p-coumaryl alcohols, especially 3,5-dimethyl-p-coumaryl alcohol, were very low compared with 3-monosubstituted p-coumaryl alcohols. These results suggest that there are three main factors of hindrance during the approach of monolignols to the active site of HRP. First, from the results of 3-monoalkoxy-p-coumaryl alcohols, it was suggested that the volume of substituents could decrease their oxidation rates. Second, from the results of 3,5-disubstituted p-coumaryl alcohols, it was suggested that local steric hindrance by the amino residues quite near the heme decreased the oxidation rates. Third, from the results of the substrates with hydrophobic substituents at their 3,5-positions, we suggested that hydrophilicity near heme would decrease their oxidation rates.  相似文献   

基于Nafion/天青A/纳米金层层自组装固定酶的过氧化氢生物传感器     

李华刚  袁若  柴雅琴  苗向敏  卓颖  洪成林 《安徽农业科学》2008,36(18)

[目的]明确基于Nafion、天青A、纳米金层层自组装固定酶制备过氧化氢生物传感器的可行性。[方法]先利用静电吸附和自组装技术将Nafion、天青A、纳米金修饰到金电极表面,然后利用纳米金吸附辣根过氧化物酶(HRP),制备过氧化氢生物传感器。采用循环伏安法和计时电流法考察该传感器的性能。[结果]在测试溶液pH值5.5、工作电位-0.3~-0.2 V、扫速100 mV/s、检测温度25℃条件下,该传感器具有良好的生物催化活性,还原峰电流与H2O2浓度在2.0×10-6~1.6×10-3mol/L范围内呈良好的线性关系,检出限为7.62×10-7mol/L。该传感器稳定性好,抗干扰力强,制备方法简单,成本低廉,对H2O2有快速灵敏的响应。[结论]该研究制备的过氧化氢生物传感器灵敏度较高,检测范围宽,检出限较低。  相似文献