草鱼MYH mRNA表达量分析中采用的内参基因稳定性比较     

周瑞雪  ;蒙涛  ;赵发兰  ;成嘉  ;宾石玉  ;张建社  ;褚武英 《广西农业生物科学》2009,(5):896-900

本研究分别以β-actin、18SrRNA和GAPDH为内参基因,采用实时荧光定量PCR对草鱼早期发育时期肌球蛋白重链（myosin heavy light,MYH）基因的mRNA表达量进行分析,并比较不同内参基因对MYH基因mRNA表达水平检测结果的准确性。研究结果表明,以β-actin和GAPDH作为内参,MYH基因mRNA表达水平完全一致,其表达量从原肠到仔鱼阶段逐次递增,仔鱼与原肠期阶段相比表达量差异显著;当采用18S rRNA作为内参时,MYH基因mRNA在发育阶段的表达量呈不稳定状态。因此,β-actin和GAPDH均可作为内参基因,用于草鱼早期发育中MYH基因mRNA的相对定量研究;而18S rRNA作为内参时,可能会对检测结果造成偏差。本研究不仅准确的揭示了草鱼MYH基因mRNA的表达特征,并且为荧光定量PCR技术在鱼类基因表达研究方面提供了有价值的参考。  相似文献   

Analysis of 18S rRNA gene of Octostigma sinensis (Projapygoidea: Octostigmatidae) supports the monophyly of Diplura     

Yun-Xia Luan  Yong-Gang Yao  Rong-Dong Xie  Yi-Ming Yang  Ya-Ping Zhang  Wen-Ying Yin   《Pedobiologia》2004,48(5-6):453-459

The phylogenetic position of Diplura within Hexapoda has been controversial. There are three major lineages in Diplura: Campodeoidea, Projapygoidea, and Japygoidea. However, most of the previous studies were restricted to Campodeoidea and Japygoidea. Until now, only preliminary morphological study on Projapygoidea was reported, and no sequence data from Projapygoidea was available. The main aim of the present study was to investigate the phylogenetic position of Octostigmatidae, one of the three families of Projapygoidea, in Diplura and to test if Diplura are monophyletic. The complete 18S rRNA gene sequences of Octostigma sinensis (Projapygoidea: Octostigmatidae) from subtropical China, together with representative species of Campodeoidea and Japygoidea, and several species of Protura and Collembola were analyzed. The phylogenetic trees were obtained by different methods (neighbor-joining, maximum parsimony, and maximum likelihood) with a chelicerate species as outgroup. Our results suggested that Octostigma was closer to the genus Parajapyx (Japygoidea: Parajapygidae) than to the representative genus of Campodeidae (Campodeoidea). All phylogenetic trees supported the monophyly of Diplura.  相似文献   

十八世纪俄罗斯园林与中国园林比较研究     

滕云  闫红伟 《山东农业大学学报(自然科学版)》2009,40(1)

中俄园林由于中俄文化的结构、形态,或是文化形成的哲学基础、思想观念的不同而形成了两大不同的类型,但是,它们都是世界园林文化的一部分,同属于世界园林艺术.本研究拟用比较的方法对中俄古典园林艺术、特别是中国明清园林与俄罗斯十八世纪古典园林进行研究,目的是促使园林工作者了解双方的优良传统和存在问题,借鉴俄罗斯园林的长处,为我国园林事业的繁荣和发展做出新的贡献.  相似文献   

一种用C18柱纯化低浓度微量寡核苷酸的新方法     

赵松子  沈向群  漆小泉 《江西农业学报》2009,21(7):9-11

通过比较4种不同的回收方法和C18硅胶4种不同的用量对回收的影响,提出了回收低浓度微量寡核苷酸的最佳方案:分别用1 mL乙腈、2 mL灭菌双蒸水和1 mL 10 mmol/L乙酸铵平衡装填10 mg硅胶的C18 Sep-Pak反相层析柱;再将寡核苷酸溶液通过C18柱;然后用1 mL 25 mmol/L碳酸氢铵洗脱C18柱,并以3000 r/m in离心2 m in;最后用1 mL 30%乙腈洗脱C18柱,将收集的溶液离心冻干后即得到纯化的寡核苷酸。该方法的回收率可达53.7%±3.0%。  相似文献   

18—19世纪中期英国交通运输的建设与农业市场的扩展     

叶明勇 《古今农业》2009,(3):19-28

18世纪中期至19世纪中期英国接连掀起公路、运河和铁路建设的高潮，三者共同形成一个全国性的交通网络，这对于沟通农业与市场的联系起了很大促进作用。农业所需物资及农产品通过发达的交通能迅速便利地交流，大大降低了成本、提高了效率。英国农民对市场反应的灵敏也有赖于此，从某种程度上讲也就刺激了农业生产。另一方面，交通运输网络的形成又促进了市场的扩大与完善，这对于英国农业，对于英国整体经济的发展都有重大的意义。  相似文献   

黑种草子提取物对小鼠酒精性肝损伤的修复作用及机制研究     

赵伟捷  孙耀贵  孙盼盼  孙娜  杨惠珍  李宏全 《中国畜牧兽医》2022,49(12):4928-4935

【目的】验证黑种草子提取物对小鼠酒精性肝损伤的修复作用并探究其机制,为其临床开发研究提供理论依据。【方法】将56只3周龄昆明小鼠随机均分为7组：空白对照组,模型组,黑种草子提取物高、中、低剂量组,黑种草子粉剂组及水飞蓟宾阳性对照组,每组8只。除空白对照组外,其余各组均以10 mL/kg 60%酒精灌胃,每天1次,连续15 d。第16天,黑种草子提取物高、中、低剂量组分别给予8、4、2 mL/kg黑种草子提取物,每天1次,连续10 d后处死小鼠。计算各组小鼠肝脏指数;检测血清中谷丙转氨酶（alanine aminotransferase,ALT）、谷草转氨酶（aspartate aminotransferase,AST）、过氧化氢酶（catalase,CAT）、超氧化物歧化酶（superoxide dismutase,SOD）和谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）活力;制备肝脏石蜡切片观察其病理变化;Western blotting检测NOD样受体热蛋白结构域相关蛋白3（NOD-like receptor thermal protein domain associated protein 3,NLRP3）、含半胱氨酸的天冬氨酸蛋白水解酶1（cysteinyl aspartate specific proteinase 1,Caspase-1）和白细胞介素1β（interleukin-1β,IL-1β）的蛋白表达水平。【结果】与空白对照组相比,模型组小鼠肝脏指数显著上升（P<0.05）,肝细胞出现明显颗粒变性,水泡变性,核溶解,界限区分不清,肝索消失;血清ALT、AST活力显著上升（P<0.05）,SOD、CAT、GSH-Px活力显著下降（P<0.05）,说明肝损伤模型建立成功,且肝脏组织中NLRP3、Caspase-1和IL-1β蛋白表达水平显著升高（P<0.05）。与模型组相比,黑种草子提取物各剂量组血清ALT、AST活力均呈下降趋势,SOD、CAT、GSH-Px活力均呈上升趋势,其中高剂量组上述各指标均呈显著差异（P<0.05）,肝脏组织结构明显改善,NLRP3、Caspase-1和IL-1β蛋白表达水平均显著下降（P<0.05）。【结论】高剂量（8 mL/kg）黑种草子提取物可显著提高肝损伤小鼠的抗氧化水平,且可以通过抑制NLRP3炎性小体的表达而减少IL-1β的合成,从而改善肝脏功能和修复受损的肝脏组织结构。  相似文献   

The impact of weight loss on circulating cytokines in Beagle dogs     

《Veterinary immunology and immunopathology》2015,163(3-4):174-182

Chronic low-grade inflammation in obesity is characterized by an increased production of pro-inflammatory and chemotactic cytokines that are contributing to insulin resistance and related co-morbidities. Cytokines act in networks and exhibit pleiotropic effects so we investigated the circulating levels of a wide array of cytokines (pro and anti-inflammatory, chemotactic and growth factors) in a canine model of weight loss. The dogs served as their own control in order to study the impact of weight loss independent of potential confounding factors, such as history of excess weight or gender. While low-grade inflammation had been previously investigated in obese dogs by measuring changes in adipokines, acute phase proteins and key pro-inflammatory cytokines, to the best of our knowledge this is the first study to evaluate how weight loss impacts a wide array of circulating cytokines.Eighteen overweight Beagle dogs were recruited (six spayed females and 12 neutered males), and none of them were grossly obese according to the body condition score (BCS). All the dogs reached an ideal weight by the end of the program. Parameters were assessed before (baseline), at mid-point (month 3) and at end-point (month 6). Plasma GM-CSF, IL-2, Il-4, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, IFNγ, IP-10, TNFα, monocyte chemotactic protein 1 (MCP-1), keratinocyte chemokine (KC) were measured with canine multiplex immunoassays. Fat mass was assessed by dual energy X-ray absorption (DEXA).Several cytokines decreased throughout the weight loss program (p < 0.01) and were correlated with the percentage of fat measured by DEXA (p < 0.05): chemotactic (MCP-1), growth factors (GM-CSF, IL-7 and IL-2), and pro-inflammatory (KC and IL-18). We could not show trends for several cytokines, possibly because their level may be lower than the assay sensitivity: anti-inflammatory (IL-4 and IL-10), and pro-inflammatory (IL-6 and TNFα).In conclusion, while our findings for several pro-inflammatory and chemotactic cytokines are in accordance with human and rodent studies, we may have identified additional cytokines, such as growth factors, related to obesity-induced low-grade inflammation. Considering the weight loss was enabled by an adjusted diet, the role of this association of cytokines in insulin resistance and related co-morbidities needs to be clarified. Our results could help better understand the cytokine biology in dogs, and as such are relevant for further elucidating the relationship between immune function and metabolism/nutrition.  相似文献   

基于改进ResNet18模型的饲料原料种类识别方法     

牛智有  于重洋  吴志陶  邵艳凯  刘梅英 《农业机械学报》2023,54(2):378-385

为了解决饲料生产过程中入仓原料种类采用人工取样感官识别所存在的问题,实现原料种类自动识别,以玉米、麸皮、小麦、豆粕、鱼粉等大宗饲料原料为研究对象,自主设计搭建了多通道入仓原料种类自动识别装置,采集饲料原料图像数据集,并使用数据增强的方法增加样本多样性。基于ResNet18网络模型加入通道注意力机制、增加Dropout函数,并嵌入余弦退火法的Adam优化器,引入迁移学习机制训练模型,构建适用于饲料原料种类识别的CAM-ResNet18网络模型。CAM-ResNet18网络模型的原料种类验证准确率达99.1%,识别时间为2.58ms。与ResNet18、ResNet34、AlexNet、VGG16等网络模型相比,模型验证集准确率分别提升0.6、0.2、3.7、1.1个百分点。针对混淆矩阵结果分析,测试集识别平均准确率达99.4%,具有较高的精确度和召回率。结果表明,构建的CAM-ResNet18网络模型在饲料原料种类识别方面具有较高的识别精度和较快检测速度,自主研发的多通道入仓原料种类自动识别装置具有实际应用价值。  相似文献   

IL-10 Gene Polymorphisms and Their Association with Immune Traits in Four Rabbit Populations     

Xiaoying WAN  Liuliu MAO  Ting LI  Lizhi QIN  Yulai PAN  Bichun LI  Xinsheng WU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):369-375

  相似文献   

PRRSV特异性肽对CSF、PRRS免疫猪IFN-Y、IL-10分泌的影晌     

郎广平  郭敏  梁志选  冯娇  孔惟博  任少敏  李彬  韩盈盈  赵建增  高英杰 《兽医大学学报》2014,(2):187-191

应用PRRSV-GP5特异性肽刺激CSF、PRRS免疫猪外周血淋巴细胞,采用EI。IsA方法测定不同时间点淋巴细胞分泌IFN-7及IL-10的水平,分析PRRSv_GP5特异性肽刺激后,淋巴细胞培养上清中细胞因子的变化规律。结果显示,CSFV高抗PRRSV低抗体组于PRRSV免疫14d,肽刺激组中IFN-7水平显著高于对照组（P〈0．05）;其他组均差异不显著（P〉0．05）。PRRSV免疫14d,细胞培养24,72h后加PRRSV—GP5肽刺激,各组中试验组与对照组相比IL-10水平明显降低（P〈0．01）;PRRSV免疫28d,各组中肽刺激组与空白组相比,IL-10水平均无显著性差异（P〉0．05）。结果表明,PRRSV-GP5肽在PRRSV免疫早期可以促进IFN-7的分泌,并抑制IL-10的产生,但在免疫后期对淋巴细胞分泌的调节作用还需进-步研究。  相似文献