Cloning and characterization of transcripts differentially expressed in the pulp of ripening papaya     

João Paulo Fabi  Franco Maria LajoloJoão Roberto Oliveira do Nascimento 《Scientia Horticulturae》2009

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Application of crop growth simulation and mathematical modeling to supply chain management in the Thai sugar industry     

Kullapapruk Piewthongngam  Supachai Pathumnakul  Kanchana Setthanan   《Agricultural Systems》2009,102(1-3):58-66

The poorly integrated cane supply planning between mills and cane growers in the Northeast of Thailand generates an excess of cane supplies that exceeds the mills’ capacity during the peak of harvest season. Each grower individually determines his/her cultivation plan by selecting planting dates and cultivars based on one’s own preference without taking into account the individual mill’s capacity and other growers’ plans. This situation causes most sugarcane grown in this area to reach its mature stage at the same period. In this study, we propose a framework of cultivation planning to cope with the problem. The focus of the cultivation plan is a long-term plan to determine the cultivation time, the cultivar selection and the corresponding prospective harvesting time window for each field such that overall sugar production is optimized.The crop growth model and a mathematical model are employed for yield simulation and optimization task. The crop growth model enables decision-makers to visualize cane production of each individual field at different dates with different cultivars and allow decision-makers to apply the mathematical programming to cultivation planning. The suggested framework has the potential to increase sugar production by 23% when compared to the traditional method.  相似文献   

农业装备物流体系的数学建模     

孙亮 《农机化研究》2009,31(2)

在分析了农业装备物流体系特点的基础上,指出了目前存在的数学模型不能完全体现和描述农业装备物流体系的特点;建立了基于面向环境时延着色Petri网(EOCTPN)的农业装备物流体系数学模型;研究了不同类型农业装备物流体系对库存总成本和长鞭效应的影响程度;解决了目前存在的无法将正向物流和逆向物流有机结合在一起的问题;有效克服了基础Petri网描述此类问题出现的"节点爆炸"现象.在仿真实验的基础上,提出了应如何构建农业装备物流体系的方法.  相似文献   

cDNA cloning and complete primary structures of myosin heavy chains from brushtooth lizardfish Saurida undosquamis and wanieso lizardfish S. wanieso fast skeletal muscles     

Mohammed Anwar Hossain  Daisuke Ikeda  Akira Nomura  Hideto Fukushima  Shugo Watabe 《Fisheries Science》2008,74(4):921-934

ABSTRACT:   The complete cDNA sequences encoding predominant types of myosin heavy chain (MYH) in the fast skeletal muscle were determined for brushtooth lizardfish Saurida undosquamis and wanieso lizardfish S. wanieso , which are used as materials for preparing high-quality surimi-based products. The cDNA consisted of 5973 and 5987 bp, respectively, and both encompassed an open reading frame encoding a polypeptide of 1936 amino acid residues. Brushtooth and wanieso lizardfish MYH showed the amino acid sequence identity of 92–93% to white croaker MYH, which was higher than that of 90% to walleye pollack MYH. The putative binding sites for ATP, actin, and regulatory and essential light chains in the subfragment-1 region of brushtooth lizardfish MYH exhibited a high identity with white croaker counterparts as well as the sequences of subfragment-2 and light meromyosin. In contrast, phylogenetic tree, constructed by the neighbor-joining method based on mitochondrial 16S rRNA gene, revealed that the two lizardfish species formed a cluster with walleye pollack, which was paraphyletic with white croaker. Therefore, a good reputation for lizardfish and white croaker to have a high thermal-gel forming ability seemed to be reflected by MYH rather than biological similarity as revealed by the mitochondrial 16S rRNA gene.  相似文献   

Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction     

Kim DG  Bae JY  Hong GE  Min MK  Kim JK  Kong IS 《Journal of fish diseases》2008,31(9):639-647

Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL⁻¹ of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g⁻¹ tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.  相似文献   

Mitochondrial DNA deletion of the brain tissue of aged rats with learning and memory deficit     

XIANG Ping  GAO Guo-quan  ZHOU Li-hua  YAO Zhi-bin 《园艺学报》2000,16(6):535-539

AIM and METHODS: The ratio of mitochondrial DNA (mtDNA) deletion was measured to find the relationship between mtDNA deletion and aged learning and memory deficit. The aged rats were divided into two groups, aged learning and memory deficit group and aged learning and memory normal group. The ratio of mtDNA deletion was measured by dilution polymerase chain reaction. RESULTS: There are deleted mtDNA (about 4834 bp) in the cerebral cortex, hippocampus and cerebellum of both young and aged rats. The ratios of deleted mtDNA were similar in the cerebral cortex,hippocampus and cerebellum of young rats (about 0.00018%). The ratio mtDNA of aged learning and memory normal rats had increased by five-fold in the cerebral cortex and hippocampus, or one-fold in the cerebellum over young rats. The ratio of aged learning and memory dificit rats had increased by one-fold in the cerebral cortex or 0.8-fold in the hippocampus or two-fold in the cerebellum over aged learning and memory normal rats.CONCLUSIONS: There was really the increase of mtDNA in aging rat brain. And this increase was double in amount in aged learning and memory deficit rats compared to the normal learning and memory aged rats. It is suggested that the mtDNA deletions in the brain regions associated with learning and memory may be contributed to the cellular and molecular mechanism of learning and memory deicit with aged rats.  相似文献   

氯虫苯甲酰胺和氟虫腈对黏虫细胞色素P450基因表达的影响     

刘俊杰  苏栩  李亚设  谢兰芬  张百重  陈锡岭 《植物保护学报》2019,46(3):563-573

为筛选出黏虫Mythimna separata参与杀虫剂解毒代谢的主效细胞色素P450基因,采用叶片浸渍法测定了用于处理黏虫3龄幼虫的亚致死浓度,通过构建转录组测序文库并结合数字基因表达(digital gene expression,DGE)对不同处理的黏虫进行测序,并运用实时荧光定量PCR(realtime quantitative polymerase chain reaction,RT-qPCR)技术验证12个P450基因的表达情况。结果表明,用于处理黏虫的氯虫苯甲酰胺和氟虫腈亚致死浓度LC_(10)分别为0.15、13.66 mg/L;对照样品、氟虫腈处理样品和氯虫苯甲酰胺处理样品分别获得59 521 504、64 838 148和41 722 990个原始序列数据,分别获得57 441 216、62 368 912和40 285 164个过滤后的序列数据;过滤后的序列长度分别为8.62、9.36和6.04 G;碱基错误率均为0.02%;Phred数值大于20、30的碱基占总碱基的百分比均高于90.59%;鸟嘌呤+胞嘧啶(guanine cytosine,GC)含量分别为47.16%、48.94%和47.55%,表明转录组测序质量较高;黏虫受氯虫苯甲酰胺胁迫后,29个P450基因表达量上调,27个P450基因表达量下调;黏虫受氟虫腈胁迫后,23个P450基因表达量上调,26个P450基因表达量下调;12个P450基因表达量的RT-qPCR技术检测结果与DGE测序文库显示的结果基本一致。  相似文献   

基于Shapley值法的奶牛产业链利润分配机制研究     

李治  胡志全 《干旱区资源与环境》2019,33(1):77-83

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The permeation barrier of plant cuticles: uptake of active ingredients is limited by very long‐chain aliphatic rather than cyclic wax compounds     

Simona Staiger  Pascal Seufert  Katja Arand  Markus Burghardt  Christian Popp  Markus Riederer 《Pest management science》2019,75(12):3405-3412

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面向冷链物流农产品品质感知的TTI动态校准方法     

张虎  张小栓  孙格格  傅泽田  马常阳 《农业机械学报》2017,48(2):314-321

时间-温度指示器(TTI)是一种可以跟踪冷链物流温度历程的智能装置,可以指示相应的农产品品质。但现有的TTI匹配条件在一定程度上限制了TTI的应用范围,即使TTI满足了传统匹配条件的要求,但应用效果也会受到农产品采收品质波动的影响。通过使用反应动力学方程和等量线方法,在原有的匹配过程中引入了TTI的校准过程,并借此推导TTI匹配所需的基本条件,以及校准过程所需的参数。结果表明,TTI匹配性判断所需的基本条件仅为农产品品质与TTI的反应活化能相等,或两者等量线存在比例关系。在此前提下,利用公式推导或等量线的绘制过程,可以求得静态校准和动态校准时所需的相关参数,经过校准后的TTI即可与对应的食品完成匹配。校准过程的引入可以显著扩大TTI的适用范围。除此之外,动态校准模型还可以缩小处于变化中的TTI和农产品对应品质之间的差值,提高TTI指示货架期的精确性。最后,通过使用两种酶型TTI、两种不同激活条件的化学型TTI和玫瑰香葡萄进行验证实验,实验结果证明经过合理校正之后TTI的等量线普遍与玫瑰香葡萄的品质等量线更接近,对其指示效果精准。  相似文献