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MB22木聚糖酶发酵条件的研究 总被引:2,自引:0,他引:2
通过因素轮换试验和正交试验,对MB22菌产木聚糖酶的培养基配方和发酵条件进行了研究。结果显示最佳培养基组成是:以玉米芯粉和麸皮为碳源,麸皮120g/l,玉米芯280g/l,(NH4)2SO44g/l,CaCO31.5g/l,Tween80,2.0g/l,MgSO4·7H2O1.5g/l;最佳发酵条件为:起始pH5.0,摇床培养温度30℃,转速160r/min,振荡培养84h。在最佳发酵条件下,MB22菌的最高产酶活力可达898.23U/ml。虽然与一些高产菌株相比该菌株的产酶能力还有待于进一步提高,但该试验结果为进一步进行微生物生产木聚糖酶的研究提供了理论依据。 相似文献
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秀珍菇营养配方的试验 总被引:3,自引:0,他引:3
本研究通过三因素三水平的正交试验表明 ,在培养基中添加VB1(2× 10 -6) ,N P K (0 1% ) ,NAA(10× 10 -6) ,最适合秀珍菇菌丝的生长 相似文献
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With a 2 millions of tons production, France is the second country in the European Union to produce durum wheat. Durum wheat production requires high grain nitrogen concentration. Irrigation and nitrogen fertilization must be managed simultaneously to maximize grain yield and also avoid low protein concentration and environmental impacts. To help advisors and farmers to better manage together these two agricultural operations and to develop innovative managements, developing a biodecisional model is an interesting possibility. However, knowledge is still missing on how farmers already managed these operations and how these two operations are linked. We developed the conceptual model for the decision part of this computer model. We performed a survey of 28 farmers conducted over the five French production areas investigating a diversity of growing conditions to identify the set of possible constraints and farmers' decision rules. To analyze the survey, we first used a general inductive approach on individual cases and then built a conceptual model of the decision with a bottom-up approach. We identified four decision sequences for fertilization (N splitting, choice of N fertilizer, rate of application, fertilization triggering) and five for irrigation (irrigation period, anticipated number of irrigation cycles, irrigation cycles organization, irrigation triggering and irrigation cycle specificities). For each operation, the first three decision sequences refer to strategic decisions. The other decision sequences refer to tactical decisions. Coupling this model with a crop model could provide guidelines for managing durum wheat in the current climatic and economic changing context. 相似文献
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667m2用15.00%安打SC16mL和3.00%阿维菌素EC100mL处理,对水稻高龄纵卷叶螟杀虫效果最好,其防效在97.00%以上。 相似文献
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Annual C input to soil is a major factor affecting soil organic carbon (SOC) dynamics. However different types of C-sources can have different behaviour, in relation to their chemical characteristics and how they interact with soil. Root-derived C, in particular, should be more efficient than other organic materials as a result of the physicochemical and biological characteristics of the surrounding environment, leading to a reduction in the C decomposition rate.To test this hypothesis, we considered a long-term experiment underway in Northern Italy since 1962, comparing permanent meadow and 6 different crop rotations over a wide range of nutrient inputs, in both organic and inorganic forms. C inputs from amendments were measured and those from crops were calculated using allometric functions and crop and residues yields. The time evolution of SOC was studied through a single-pool, first-order kinetic model, allowing the estimation of humification coefficients for residues, roots, farmyard manure and cattle slurries.The highest value of the humification coefficient was estimated for farmyard manure, which confirmed its high efficiency in stabilising SOC content. Root C presented a humification coefficient 1.9 times higher than above-ground plant materials while slurries were intermediate, with a humification coefficient roughly half that of farmyard manure and even lower that of roots.The quality of C input thus seems of fundamental importance for evaluating the sustainability of different cropping systems in terms of SOC dynamics. 相似文献
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