日本锦鲤秋季人工育苗及其离子轰击实验初报     

杨火盛 《福建水产》2009,(2):51-53

本文报道了2006年日本锦鲤秋季人工繁殖、育苗及其离子轰击日本锦鲤水花实验结果。8尾性成熟亲鱼,在14．5℃-23．2℃水温条件下,经强化培育后,采用地欧酮（DOM）、绒毛膜促性腺激素（HCG）催熟和催产,其中1尾雌鱼产卵。受精卵孵出水花1000尾。在15．2℃～21．0℃水温条件下,分离子轰击实验组、对照组和普通组共3组对日本锦鲤水花进行60d培育,成活率分别为66％、93％、51％;抽测普通组鱼苗,平均全长为2．4cm。  相似文献   

Population structure of the Japanese eel Anguilla japonica as examined by mitochondrial DNA sequencing   总被引：1，自引：0，他引：1  

Satoshi Ishikawa  Jun Aoyama  Katsumi Tsukamoto  Mutsumi Nishida 《Fisheries Science》2001,67(2):246-253

SUMMARY: To examine the population structure of the Japanese eel Anguilla japonica , mitochondrial DNA sequence analysis was made for various samples of glass eels that might reflect genetic characteristics of spawning aggregates. The mitochondrial DNA from a total of 51 glass eels collected at Tanegashima Island, Kanagawa and Ibaraki in different periods within an inshore migrating season was sequenced for a 615 base-pair fragment from the tRNA^Thr gene to the central part of the control region. The DNA region was so variable that no individual was found to possess the same sequence, although average sequence differences within samples (1.07–1.63) were not large. Average sequence differences between samples (1.22–1.57) were comparable to those within samples, suggesting no genetic heterogeneity among samples. Tree analysis of the sequences showed neither a geographical nor temporal structure of population. Furthermore, although all DNA sequences from the present study were different from one another, two sequences were found to be the same with those reported from individuals collected in different localities in different years. Altogether, the present mitochondrial DNA sequence analysis of glass eels did not provide any evidence for genetic subdivision of the Japanese eel population.  相似文献   

益生菌的应用对鳗鲡池塘水质变化的影响   总被引：1，自引：0，他引：1  

石和荣  林敏  马江耀  杜君裕  蔡秩强 《水产养殖》2010,31(9):26-29

在鳗鲡养殖池中应用益生菌,研究其对池塘水质变化的影响。结果表明,在鳗鲡养殖池中添加芽胞杆菌、光合菌及EM菌,能有效降低养殖水体的氨氮(NH4+-N)、亚硝酸盐氮(NO2--N)及化学耗氧量,有利于水质净化和微生态环境的修复。  相似文献   

鲐鱼幼鱼耳石日轮的初步观察与研究   总被引：7，自引：0，他引：7  

陈卫忠 《水产学报》1996,20(2):139-143

对长江口海区鲐鱼幼鱼耳石标本的扫描电镜观察发现，鲐鱼耳石上存在着宽度为数微米到十几微米的细微轮纹，通过对其中３尾鲐鱼的耳石不同放大倍数图像的拍摄，再根据照片对耳石上轮纹和耳石轮纹辐射半径的测量、计数和计算，得出３尾鲐鱼耳石轮纹的平均宽度分别为９．５２、８．５２和８．５７微米，耳石轮纹辐射半径分别为１２６２．２、１１６４．８和１１８７．５微米，由此计算得出的耳石轮纹数分别为１３３、１３７和１３９轮。  相似文献   

Effects of six alternative lipid sources on growth and tissue fatty acid composition in Japanese sea bass (Lateolabrax japonicus)     

Min Xue  Lin Luo  Xiufeng Wu  Zelin Ren  Peng Gao  Yu Yu  Gary Pearl 《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):206-214

Ten-week experiment was carried out on Japanese sea bass (5.87 ± 0.02 g) to study the effects of replacement of fish oil with six alternative lipid sources: pork lard, PL; beef tallow, BT; poultry fat, PF; soybean oil, SO; corn oil, CO; and a mixed-fat (MF: tallow, 60%; soy oil, 20%; fish oil, 20%) on growth performance and fatty acid (FA) composition in fillet and liver. Seven isoenergetic and isonitrogenous experimental diets were formulated, containing 10% of added lipid. Fish oil was used in control diet, which was substituted by 50% with the alternative lipid sources in the other six diets.

Weight gain (WG), specific growth rate (SGR), Feed conversion ratio (FCR) feed intake and hepatosomatic index (HSI) of fish fed the experimental diets were not significantly different (P > 0.05). Protein efficiency ratio (PER) in fish fed the PF diet were significantly lower than those of fish fed SO and CO diets. Significant differences in carcass moisture and lipid contents of carcass and liver were observed among fish fed the dietary treatments. Generally, the fatty acid composition of fish fillets and livers reflected the dietary FA composition.  相似文献   

Effect of low molecular weight fish protein hydrolysate on growth performance and IGF‐I expression in Japanese flounder (Paralichthys olivaceus) fed high plant protein diets     

K. Zheng  T. Xu  C. Qian  M. Liang  X. Wang 《Aquaculture Nutrition》2014,20(4):372-380

Seven isonitrogenous and isoenergetic experimental diets were formulated to investigate the effect of low molecular weight fish protein hydrolysate (FPH) in diets on growth performance, feed utilization and liver IGF‐I mRNA levels in Japanese flounder (38.80 ± 1.11 g) fed with high plant protein diets. Fish meal protein was, respectively, replaced by 6% (FPH6), 11% (FPH11), 16% (FPH16), 21% (FPH21), 26% (FPH26) FPH of total dietary protein. FPH diets contained a constant high level of plant protein (690 g kg^?1) from soybean meal. As a positive control diet, FM2 contained about 590 g kg^?1 plant protein and 410 g kg^?1 fish meal protein, while negative control diet FM1 contained about 690 g kg^?1 plant protein and 310 g kg^?1 fish meal protein. The expression levels of liver IGF‐I mRNA were evaluated using real‐time PCR normalized against the 18S rRNA gene. The results showed that moderate low molecular weight FPH (FPH11) improved growth performance and protein retention. Fish fed with FPH11 and control diet FM2 had similar growth and feed utilization, while high‐level low molecular weight FPH did not improve growth performance and protein retention, and depressed liver IGF‐I mRNA expression in Japanese flounder.  相似文献   

Qualitative and quantitative changes of F<Subscript>o</Subscript>F<Subscript>1</Subscript>-ATPase in Japanese flounder and red sea bream associated with rearing temperatures     

Shiro Itoi  Koki Ikeguchi  Masaki Kaneniwa  Ryuji Kuwahara  Ichiro Ohara  Noriko Ishida  Michiaki Yamashita  Shugo Watabe 《Fisheries Science》2007,73(2):429-439

ABSTRACT:   Fast skeletal muscles of Japanese flounder Paralichthys olivaceus and red sea bream Pagrus major were examined for quantitative and qualitative changes of mitochondrial ATP synthase (F_oF₁-ATPase) in association with rearing temperatures. The specific activities of F_oF₁-ATPase from Japanese flounder reared at 10°C, 15°C and 25°C for 4 weeks were determined to be 81 ± 11, 74 ± 13 and 83 ± 11 nmol/min·mg mitochondrial protein, respectively. The corresponding activity from red sea bream reared at 8°C for 5 weeks was determined to be 65 ± 9 nmol/min·mg mitochondrial protein, which was higher than 33 ± 9 nmol/min·mg mitochondrial protein in fish reared at 23°C. The contents of α- and β-F₁-ATPase in total mitochondrial proteins were not significantly different between fish reared at different temperatures for the two fish species. However, the contents of β-F₁-ATPase in the total fast skeletal muscle extracts, prepared from Japanese flounder reared at 10°C, were 2.1- and 2.9-fold higher than those for fish reared at 15°C and 25°C, respectively. The corresponding content from red seabream reared at 8°C was 2.2-fold higher than that for fish reared at 23°C. Therefore, the changes in F_oF₁-ATPase depending on rearing temperatures were species-specific.  相似文献   

Lipid content and fatty acid composition of muscle,liver, ovary and eggs of captive-reared and wild silver Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis> during artificial maturation     

Yuichi Ozaki  Hidehiro Koga  Takako Takahashi  Shinji Adachi  Kohei Yamauchi 《Fisheries Science》2008,74(2):362-371

ABSTRACT:   Changes in lipid content and fatty acid composition of muscle, liver and ovary of captive-reared and wild silver Japanese eel Anguilla japonica were examined during artificial maturation induced by salmon pituitary homogenate (SPH) injections. Although the relative levels of n-3 and n-6 highly unsaturated fatty acids (HUFA) in liver and ovary were higher than in muscle in both captive and wild silver eels before SPH injection, these tended to decrease with maturation. The relative levels of n-6 HUFA in muscle, liver, ovary and eggs of wild silver eels were remarkably higher than those in captive eels. Therefore, we attempted to alter the ratio of n-6 HUFA in eggs by feeding eels a diet supplemented with linoleic acid-rich plant oil. Although the percentage of n-6 polyunsaturated fatty acids to total fatty acids in eggs of eels fed the supplemented diet was similar to wild silver eels, the percentage of n-6 HUFA remained remarkably lower than in wild silver eels. Hence, it appears that the supplemented diet affected the fatty acid composition of eggs, but did not result in much conversion of linoleic acid to its higher homologs in eels.  相似文献   

日本黄姑鱼精子生理特性及超低温冷冻保存研究   总被引：4，自引：1，他引：4  

闫文罡  章龙珍  庄平  刘鹏  江琪 《海洋渔业》2008,30(2)

对日本黄姑鱼精子部分生理特性及其超低温冷冻保存技术进行了研究。结果表明,日本黄姑鱼精液pH值为7.0~7.5,精子密度为(11.23±2.78)×109/m l,精子寿命(229.33±17.16)s。在盐度为30~35,pH为7.5~8.5时,精子的活力最高,分别达到(88.33±2.89)%和(88±4.33)%。精子在室温(25℃)条件下,可存活24 h;在低温(4℃)条件下可以存活36 h。以D液作为稀释液,20%的乙二醇作为抗冻剂,利用快速降温法对精子进行超低温冷冻保存,38℃水浴快速解冻,解冻后的精子获得最高的活力为(47±5.78)%。  相似文献   

Mitochondrial DNA sequence variation of Japanese anchovy <Emphasis Type="Italic">Engraulis japonicus</Emphasis> from the Yellow Sea and East China Sea   总被引：4，自引：0，他引：4  

Zi?-Niu?Yu  Xiao?-Yu?KongEmail author  Tian?-Hui?Guo  Yan-yan?Jiang  Zhi-meng?Zhuang  Xian-shi?Jin 《Fisheries Science》2005,71(2):299-307

Sequence variation of a fragment of the mitochondrial cytochrome b (cyt b) and cytochrome c oxidase subunit I (COI) gene was examined using polymerase chain reaction and direct sequencing among three populations of Japanese anchovy Engraulis japonicus from the Yellow Sea and East China Sea. A total of 24 and 47 nucleotide sites were detected variable defining 29 and 32 haplotypes in cyt b and COI data, respectively. All variable sites except one in COI were silent in the two sets of sequences. The Ewens-Watterson test indicated that the observed allelic configurations in both data sets were in full agreement with neutral expectations. The variation level was high, with h=0.957±0.018, π=0.644±0.387 (%) in cyt b data set and h=0.958±0.021, π=0.640±0.386 (%) in COI data set, respectively. However, at the population level, Fst values between pairs of populations were not significantly different from zero (P>0.05) in both data sets. The analysis of haplotype frequency distribution showed no significant differences among populations. Similarly, the analysis of the partitioning of molecular variance indicated that all or almost all of the genetic variation was distributed within populations. Based on the data from this study, the existence of separate genetic stocks in this area were not detected. Mixing of stocks to some extent in migration cycle and dispersal capacity of anchovy’s planktonic larvae could be the reasons for genetic homogeneity in this species in the Yellow Sea and East China Sea.  相似文献