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71.
W. Van Der Werf R. Rabbinge W. Heijbroek 《European journal of plant pathology / European Foundation for Plant Pathology》1986,92(1):33-42
A simulation model was developed for the spring invasion of the beet cyst nematode,Heterodera schachtii Schmidt, into sugarbeet roots, according to the state variable approach. This model describes the processes of egghatch, emergence of second stage larvae from cysts, migration to roots and penetration into roots quantitatively, using published data.In 1983 a field experiment was conducted to test this model.H. schachtii cysts were introduced at depths 6–29 cm in PVC-cylinders, buried in the soil. The rooting depth of sugarbeet seedlings, growing in these cylinders, was limited to 5 cm by 50 m mesh nylon gauze. Every 10 days the second stage larvae, which had penetrated into the roots of these seedlings were counted. After 50 days, about 40% of the eggs had hatched. More than 20% of the emerged larvae penetrated if the cysts had been buried undeeply, and only 4% if the cysts had been buried at 29 cm depth.The model predicted the course of penetration into the root during the first 40 days with reasonable accuracy (r2=0.79), but in the 5th period of 10 days the model made an overestimation of more than 100%. Egghatch after 50 days was correctly simulated. The differences in penetration into the root between the model and the experiment might result from an oversimplified simulation of the penetration success or the neglection of mortality of second stage larvae. Detailed experiments should be done to provide better parameters for these factors.Samenvatting Volgens de toestandsvariabele-benadering werd een simulatiemodel ontwikkeld van de voorjaarspenetratie van het bietecystenaaltje. Het model beschrijft aan de hand van literatuurgegevens het uikomen van de eieren, het verlaten van de cyst door de larven, de migratie naar en de penetratie in de wortel.In 1983 werd een veldproef uitgevoerd om het model te toetsen. Cysten vanH. schachtii werden op 5 dieptes tussen 6 en 29 cm ingegraven in PVC-cylinders, welke waren verzonken in de bodem. De bewortelingsdiepte van de suikerbiete-zaailingen die hierin groeiden werd beperkt tot 5 cm door nylon gaas van 50 m maaswijdte. Elke 10 dagen werden de larven geteld die in de wortels van deze plantjes waren gepenetreerd. Na 50 dagen was 40% van de eieren uitgekomen. Meer dan 20% van de gelokte larven penetreerden als de cysten ondiep waren ingegraven, en slechts 4% als de cysten op 29 cm diepte waren ingegraven.Gedurende de eerste 40 dagen werd het verloop van de penetratie in de wortel met redelijke nauwkeurigheid door het model voorspeld (r2=0.79). In de 5e periode van 10 dagen maakte het model echter een overschatting van meer dan 100%. Het uitkomen van de eieren werd correct gesimuleerd. De verschillen in penetratie tussen het model en de proef zouden het gevolg kunnen zijn van een oververeenvoudigde simulatie van het penetratiesucces of van het verwaarlozen van de mortaliteit van de migrerende larven. Betere gegevens hierover zullen moeten komen uit detailproeven. 相似文献
72.
J. Rashid D. J. Weiss S. K. Maheswaran M. P. Murtaugh 《Veterinary research communications》1996,20(6):519-531
Local and systemic activation of coagulation is frequently associated with bacterial sepsis. The coagulopathy is due, at least in part, to expression of tissue factor (TF) by monocytes and macrophages. The purpose of this study was to evaluate the expression of procoagulant activity by bovine alveolar macrophages, leukocytes and platelets, and to determine the relative potency of three chemical inhibitors of TF expression (pentoxifylline, retinoic acid, and cyclosporin A). Bovine alveolar macrophages were stimulated with lipopolysaccharide (LPS) derived from Pasteurella haemolytica or recombinant bovine tumour nervous factor (TNF) and dose- and time-dependent effects on TF expression were studied. LPS and TNF induced TF expression in alveolar macrophages and LPS treatment of whole blood induced TF expression in mononuclear cells. Neutrophils and platelets also expressed procoagulant activity, but this activity was not inhibited by anti-bovine TF monoclonal antibody. Pentoxifylline (40 mol/L), retinoic acid (0.01 mmol/L) and cyclosporin A (0.08 mol/L) inhibited TF expression when added concurrently with LPS or TNF, but not when added 4 h after stimulation. TF mRNA was not detected in unstimulated alveolar macrophages by Northern blot analysis. In contrast, exposure to LPS or TNF for 6 h induced marked expression of TF mRNA, which was inhibited by treatment with pentoxifylline, retinoic acid and cyclosporin A. Expression of TNF by alveolar macrophages stimulated with LPS was also inhibited by these compounds. Our results indicate that procoagulant activity expressed by alveolar macrophages and monocytes is associated with expression of TF, whereas procoagulant activity expressed by neutrophils and platelets is not. The concentrations of pentoxifylline and retinoic acid necessary for inhibition of TF expression in vitro may not be achievable in vivo owing to their toxic effects. However, the in vitro concentration of cyclosporin A that inhibited TF expression did not exceed the plasma concentration observed in humans, and therefore may be useful for inhibition of TF expression in vivo.Abbreviations BAL
bronchoalveolar lavage
- LPS
lipopolysaccharide
- cDNA
cloned deoxyribonucleic acid
- cAMP
cyclic adenosine monophosphate
- GAPDH
glyceraldehyde phosphate dehydrogenase
- mRNA
messenger ribonucleic acid
- TF
tissue factor
- TNF
tumour necrosis factor
- DPBS
Dulbecco's phosphate-buffered saline 相似文献
73.
Pathogenic mechanisms of caprine arthritis-encephalitis virus 总被引:1,自引:0,他引:1
E. G. Mdurvwa P. O. Ogunbiyi H. S. Gakou P. G. Reddy 《Veterinary research communications》1994,18(6):483-490
Goats infected with caprine arthritis-encephalitis virus (CAEV) show chronic arthritis and cachexia, which are progressive in nature. The immunopathogenic mechanisms responsible for these progressive clinical symptoms have not been fully elucidated. Various haematological and immunological parameters were evaluated in experimentally-infected goats showing typical signs of CAEV-induced disease. Infected goats showed recurrent lymphocytosis that may be due to constant presentation of antigen by infected cells of a monocyte/macrophage lineage. The serum alkaline phosphatase and -glutamyl transferase concentrations were elevated in infected goats, a characteristic of hepatic and bone disorders. All other serum chemistry parameters were similar between infected and control goats. Importantly, the serum tumour necrosis factor- (TNF-) levels were higher in infected goats. The cachexia seen in infected goats may be at least partly due to altered metabolism as a result of prolonged elevation of serum TNF- levels. Depressed natural killer cell activity was observed in infected goats and may contribute towards the establishment of a persistent infection with CAEV.Abbreviations AIDS
acquired immunodeficiency syndrome
- CAEV
caprine arthritis-encephalitis virus
- GGT
-glutamyl transferase
- HBSS
Hanks' balanced salt solution
- HIV
human immunodeficiency virus
- NK
natural killer
- PBMC
peripheral blood mononuclear cells
- PCR
polymerase chain reaction
- SAP
serum alkaline phosphatase
- TNF
tumour necrosis factor 相似文献
74.
G. Jager H. Velvis 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(4):467-478
The position of plants withRhizoctonia solani sclerotia (black scurf) on progeny tubers was mapped for an experimental field at Haren where potatoes were grown continuously and in rotation with other crops for five successive years, and for another field at Borgercompagnie with a 12 frequency of potatoes during three potato crops. Initially, the distribution of plants with black scurf on both fields was rather dense and homogeneous. In the following years the distribution became heterogeneous and patchy. The local decline ofR. solani AG 3 (the common potato pathogen) in Haren was apparently caused by an unknown factor selectively suppressingR. solani AG 3, while simultaneouslyR. solani AG 5 increased in mass. This AG 5 type proved to be an inferior competitor of AG 3 on the potato plant in a laboratory experiment. The specificR. solani antagonistVerticillium biguttatum did not play a role. A similar factor could have reduced the formation of black scurf in the experimental field at Borgercompagnie, whereV. biguttatum was also too infrequent to account for the decline.R. solani AG 5 was not present here and could not indicate the presence of a selective factor against AG 3. 相似文献
75.
76.
A general bio-economic model for beef cattle production was used to define breeding objectives for Charolais cattle to be utilized in a variety of linked production systems. Economic weights were calculated for 16 traits (some with both direct and maternal components) in three production systems (pure-breeding and terminal crossing with beef or dairy cows) and two marketing strategies (sale or fattening of weaned surplus calves). Economic weights for the total breeding objective were calculated as weighted averages, where weights were numbers of cows expected to be mated with Charolais bulls in each production system and marketing strategy. Results suggest that the direct component of calving performance and cow longevity were of primary economic importance in all systems. Conception rate of cows and weaning weight reached about 50% of the standardized economic weight of calving performance in purebred systems with sale of weaned calves, whereas in purebred systems with fattening the economic importance of the direct component of cow conception rate, losses at calving, mature weight of cows, weaning weight, and fattening traits were of equal importance (each approximately about 20% that of calving performance). In terminal crossing systems, weaning weight was important when calves were sold at weaning, and fattening traits were important for systems selling fattened animals. The bio-economic model performed well under the conditions of this demonstration and could easily be customized for other applications. 相似文献
77.
旱地保护性耕作地表径流和土壤水分平衡模型 总被引:12,自引:3,他引:12
在田间试验和对现有径流模型及土壤水分平衡模型改进的基础上,建立了适用于保护性耕作的地表径流和土壤水分平衡模型。该模型以日为步长,根据气象数据、土壤水分状况、作物生长发育及耕作管理措施,模拟不同耕作管理体系下地表径流和田间水分平衡的变化。针对保护性耕作的特点,主要对径流曲线数字(USDA—Curve Number)法进行了改进,在PERFECT模型的基础上增加了坡度和降雨强度两因素的影响,从而使模型较全面地考虑了残茬覆盖、耕作、坡度及降雨强度等多种因素对径流的影响;采用简单实用的Priestley—Taylor公式计算潜在蒸散量,并考虑作物覆盖与残茬覆盖对土壤蒸发与作物蒸腾的影响。通过田间径流试验和根层有效贮水量测定数据的验证,证明了地表径流和土壤水分的模拟值与实测值比较接近。 相似文献
78.
宜吉共建黄河壶口瀑布旅游风景名胜区的合作方案研究 总被引:2,自引:0,他引:2
黄河壶口瀑布旅游风景名胜区由于地跨晋陕两省,长期以来以行政为单元进行独立开发的模式制约了景区的发展。本文在认真分析了现行的开发模式和三种可供选择的方案之后,提出了一种可行的创新方案。 相似文献
79.
灰色灾变理论在宁南山区干旱气候预测中的应用 总被引:15,自引:2,他引:15
在分析宁南山区干旱气候特征及其对农业生产影响的基础上 ,借助灰色灾变理论分别建立了春旱、夏旱、秋旱、春夏连旱、夏秋连旱、全年旱等六种干旱类型的 GM( 1 ,1 )预测模型 ,并对 2 0 0 0~ 2 0 2 0年的干旱气候做出预测。经对 2 0 0 0年和 2 0 0 1年预测结果验证 ,吻合性良好。此外 ,提出了宁南山区抗旱防旱的综合农业措施。研究结果可对广大旱区抗旱减灾 ,促进农业生产发展提供科学指导。 相似文献
80.
关中平原小麦产量对气候变化区域响应的评价模型研究 总被引:4,自引:0,他引:4
根据关中地区宝鸡、西安、渭南与咸阳 4地 (市 )的 1 949~ 1 999年的逐年小麦单产记录序列以及 4地 (市 )的气象观测站点自建站以来至 2 0 0 0年近 5 0年的气象记录序列 ,对关中地区小麦产量与年均温、年降水作相关分析 ;探讨了关中地区小麦单产对气候变化区域响应的评价模型。结果发现 :关中平原气候具有暖干化趋势 ;随着气温变暖 ,小麦产量增加幅度减小 ;小麦产量对降水波动的响应比对气温波动的响应显著。 相似文献