6种番茄叶表皮制片方法比较     

宋艳波  杨云浩  段鹏军  王嘉浩  魏丝晴  张亚龙 《农学学报》2022,12(1):45-52

结合番茄叶片结构特点,采用6种表皮制片技术对番茄叶片下表皮进行装片,并对不同方法的制片过程和观察效果进行比较研究.结果表明,番茄叶片薄,表皮为单层细胞,其上着生有丰富表皮毛,下表皮与疏松的海绵组织相邻;表皮撕取法和透明胶带粘取法可以真实地观测气孔大小、密度等静态性状指标和气孔开度等动态行为;因番茄叶柔软,刮片法对操作要...  相似文献   

31个杂交杨无性系对青杨叶锈病的抗性评价   总被引：4，自引：0，他引：4  

万雪琴  夏新莉  尹伟伦 《林业科技》2006,31(5):22-25

在大田条件下对从北美新引种的31个杂交杨无性系对青杨叶锈病的抗性进行调查和评价的结果表明:所引进无性系对杨树叶锈病的抗性表现出很大的差异,从高度抗病到高度感病均有,但没有免疫的无性系。HE-2,XMH-10,XMH-9,HE-1,XMH-1,XMH-11,XMH-12,XMH-2,MH-8,XMH-7这10个无性系表现出高度抗病性,MH-9,DMH-3,MH-10,MH-4,MH-1,MH-6,MH-7,MH-2这8个无性系表现为高度感病。DN、NE杂交的大多数无性系有较高的抗病性,而TD、TN杂交的绝大多数无性系为感病或高度感病。同一杂交组合的不同无性系间,其抗性存在很大差异,有高抗,也有高感。  相似文献   

悬铃木叶片再生体系的建立   总被引：9，自引：0，他引：9  

王磊  李红双  蔺娜  崔德才 《林业科学》2004,40(1):58-63

以悬铃木为试验材料 ,系统地研究了激素浓度、叶片生理状态、光照条件等诸多因素对叶片再生的影响 ,建立了悬铃木叶片外植体的不定芽高频再生体系。研究结果表明 ,以顶部充分伸展的 4 0d叶龄的无菌苗叶片为外植体 ,再生效果最好 ,将此类叶片放在含 1 5mg·L- 1 6 -BA ,0 5mg·L- 1 IBA和 0 5mg·L- 1 KT的MS分化培养基上 ,暗培养 7d后转到光下培养 ,15d后便可见到不定芽不经过或经过很少的愈伤组织阶段 ,直接从叶片上分化产生 ,出现的高峰期在接种后的 2 0～ 30d ,芽分化率高达 98%以上。待小芽长至 1～ 2cm ,将其从叶片上切下 ,转到芽伸长培养基 (MS 0 3mg·L- 1 6 -BA 0 0 5mg·L- 1 NAA 30mg·L- 1 Ad)上使小芽长大成苗 ,最后移到生根培养基(1 2MS 0 1mg·L - 1NAA)上 ,最终得到完整的植株。该再生体系可作为基因转化的受体系统。  相似文献   

高光谱遥感森林叶面积指数估测研究现状   总被引：5，自引：0，他引：5  

赵丽芳  谭炳香  杨华  李增元 《世界林业研究》2007,20(2):50-54

文中简要概述了叶面积指数和高光谱遥感的概念, 分析比较了高光谱遥感与传统的宽波段遥感估测森林叶面积指数的优势, 以及利用高光谱遥感数据估测森林叶面积指数的常用方法, 并对目前高光谱遥感估测森林叶面积指数存在的问题行了讨论。  相似文献   

Effects of N, NP, and NPKS fertilizers applied to four-year old hybrid poplar plantations   总被引：1，自引：0，他引：1  

R. van den Driessche  Barbara R. Thomas  David P. Kamelchuk 《New Forests》2008,35(3):221-233

Mineral fertilizers were applied to adjacent plantations of 2, 4-year old, hybrid poplars: clone 27 (Northwest, P. deltoides × P. balsamifera) and 794 (Brooks, P. deltoides × P. × petrowskyana), in north eastern Alberta. Fertilization was done in May 2003 to see whether growth rates could be increased and rotations shortened. Three fertilizers (N, NP and NPKS + Cu + Zn) were applied at each of three rates (supplying N at 0, 100 and 200 kg ha⁻¹) in a factorial randomized block design to the two separate plantations. Fertilization with 200 kg ha⁻¹ N and 100 kg ha⁻¹ P increased volume growth of clone 27 by 1 m³ ha⁻¹ year⁻¹ over 2 years. Clone 794 showed no volume response to fertilization, but produced 8.7 m³ ha⁻¹ more than clone 27. Leaf area, dry mass and nutrient concentrations of both clones increased in the first year after fertilization, showing that fertilizer uptake occurred. Decrease in leaf size between 2003 and 2004 was affected by fertilizer level in clone 794. There were differences between some nutrient concentrations in the soils occupied by the two clones, and clone 794 had higher leaf concentrations of N, K, Ca, S, Mn, Zn, B and Mo than clone 27. Fertilization of 4-year old plantations of either clone was unwarranted, and planting clone 794 would be likely to provide greater yield than planting and fertilizing clone 27.  相似文献   

抗蒸腾剂对刺槐、核桃水势日变化的影响     

邓春娟  郭建斌  梁月 《林业科技开发》2008,22(6)

通过对研制出的5种抗蒸腾剂F1、F2、F3、F4、F5的3种浓度0.05%、0.1%、0.5%在刺槐、核桃幼苗上的应用效果分析,得出如下结论:5种抗蒸腾叶面肥均能抑制刺槐、核桃叶水势的下降。F1处理对两种苗木叶水势下降抑制作用最小,F5处理对两种幼苗叶水势的下降抑制作用最大。各个浓度处理中,0.5%的F5处理对两种幼苗叶水势的下降抑制作用最显著。  相似文献   

Vertical and leaf-age-related variations of nonstructural carbohydrates in two alpine timberline species, southeastern Tibetan Plateau     

Ming-cai Li  Gao-qiang Kong  Jiao-jun Zhu 《Journal of Forest Research》2009,14(4):229-235

Spatial pattern of nonstructural carbohydrates (NSC) was analyzed in two alpine timberline species (Abies georgei and Juniperus saltuaria) growing at the timberline in Sergyemla Mountain, southeastern Tibetan Plateau. We aimed to examine the effect of canopy height and leaf age on balance between carbon uptake and consumption of timberline tree species in extremely environmental condition. The results showed that no significantly vertical variation in sugars, starch or NSC (soluble sugars plus starch) in A. georgei was found for any aged needles. Also, there were no significant differences among vertical gradients in both current and last-year leaves for J. saltuaria. However, different-aged needles/leaves showed significant differences in NSC concentrations in both A. georgei and J. saltuaria. For A. georgei, needle mean NSC across vertical canopies showed a significant increase from current-year to 2-year needles (P < 0.05), followed by a gradual decrease from 2- to 5-year needles (P < 0.05), whereas for J. saltuaria, last-year leaves had significant higher NSC except sugars compared with current-year leaves across canopies (P < 0.01). The observed trends of NSC along vertical canopy heights and leaf ages suggested that, in extreme environmental condition, not only light-induced carbon acquisition in photosynthesis but also carbohydrate export from leaves should be taken into account to explain the spatial pattern of NSC.  相似文献   

Senescence of Top Three Leaves in Field‐Grown Rice Plants     

《Journal of plant nutrition》2013,36(12):2453-2468

Abstract

The top three leaves play important roles in biomass production and grain yield of rice (Oryza sativa L.) crop since the three leaves not only assimilate majority of carbon for grain filling during ripening phase, but also provide large proportion of remobilized‐nitrogen (N) for grain development during their senescence. The objectives of this study were to (a) compare senescence of the top three leaves and (b) compare the changes in N, chlorophyll, and ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco) contents of the top three leaves after their full expansion in field‐grown rice plants. When the basis of comparison among the top three leaves was plant age in terms of days after transplanting (DAT), senescence generally started earliest in ?3rd leaf, intermediate in ?2nd leaf, and latest in flag leaf. If the basis of comparison among the top three leaves was leaf age in terms of days after full leaf expansion (DAFE), it was not clear which leaf senesced earlier. Senescence rate was generally greatest in flag leaf, intermediate in ?2nd leaf, and smallest in ?3rd leaf. Ribulose‐1,5‐bisphosphate carboxylase/oxygenase content declined earlier, and at a faster rate than N and chlorophyll contents during the senescence of all top three leaves. Correlation analysis indicated a close relationship between N and chlorophyll contents. Ribulose‐1,5‐bisphosphate carboxylase/oxygenase content correlated with N content better than with chlorophyll content. The suitability of N, chlorophyll, and Rubisco contents for quantifying the leaf senescence of field‐grown rice plants is discussed.  相似文献   

有机肥施用方式对柑橘园土壤肥力和柑橘养分、品质的影响   总被引：2，自引：0，他引：2  

褚长彬  吴淑杭  张学英  周德平  范洁群  姜震方 《上海农业学报》2012,28(1):65-68

为提高上海崇明柑橘的品质和产量,研究了不同有机肥施肥量和施肥深度对柑橘园土壤肥力和柑橘养分、品质的影响.结果表明:与未施有机肥处理相比,施用有机肥可以不同程度地增加土壤中速效N、有效P和有效K的含量,最好的处理分别高出对照20.9％、24.9％和44.4％;并能提高土壤有机质的含量,最好的处理高出对照16.2％;同时减小土壤容重,降低土壤的pH,从而提高土壤肥力;施用有机肥可以增加土壤中微生物的数量,并且能提高柑橘叶片中全氮和全钾的含量,改善柑橘果实品质.  相似文献   

金铁锁居群在水分限制下的叶解剖结构研究(英文)     

屈燕  虞泓  张时刚 《农业科学与技术》2012,(1):134-138,154

[目的]对金铁锁居群在水分限制下的叶解剖结构进行研究。[方法]选取引种至实验大田的多年生草本植物金铁锁的2年生实生苗为研究对象,在给水和不给水2种条件下,采用常规石蜡切片法和印迹法,对2个居群的叶解剖结构及气孔的生理特性进行了对比研究。[结果]在冬春季干旱期,不给水栽培条件下的金铁锁表现为叶片上角质层变厚,栅栏组织和海绵组织比值增加,维管束直径增加,主脉木质部维管数增加;叶气孔口变小,气孔密度增加等变化,可以将这些性状的可塑性作为金铁锁居群对水分亏缺作出反应的重要指标。同时,这些指标的变化幅度存在居群间差异。丽江阿西居群在不给水的栽培条件下,叶解剖结构和气孔特性变化显著,从而可能使该居群对干旱环境具有更强的适应能力。[结论]对筛选抗旱金铁锁种质资源及探讨居群在自然干旱条件下的适应性分化具有参考价值。  相似文献