乙型脑炎病毒SA1444-2株E蛋白C端片段的克隆及原核表达     

李春利  王云龙  李晨阳  李玉林  田璐  赵瑞红  李玲玲 《安徽农业科学》2008,36(23)

[目的]为进一步研制检测乙脑抗体试剂盒奠定了基础.[方法]以本实验室已构建的重组质粒pET-E(E为乙脑疫苗株SA14-14-2株E蛋白)为模板,利用PCR扩增乙型脑炎病毒E蛋白基因3'端部分片段,克隆入原核表达栽体pET-32a( ),转化大肠杆菌BL21(DE3),经IPTG诱导表达后,SDS-PAGE电泳分析表达产物.[结果]所表达的融合蛋白分子量约为38KD,表达产物以水溶形式存在,37℃,诱导4 h的诱导培养条件最佳,表达量约占菌体总蛋白的20%.表达产物经Ni-NTA亲和层析法纯化,获得了纯度较高的目的蛋白.ELISA检测表明,表达的蛋白能与猪乙脑病毒抗体血清发生特异性反应,具有很好的抗原性.[结论]基因工程表达的JEV E蛋白有望成为重要的实验室诊断抗原.  相似文献   

镍对水稻生长发育影响和残留规律的研究     

康立娟  刘迎春  李立英  白青山 《农业环境科学学报》1997,(3)

本文对吉林省砂壤水稻土进行镍（ＮｉＳＯ４·６Ｈ２Ｏ）的不同浓度对水稻生长发育影响和残留规律的盆栽试验研究。结果表明，镍对水稻的毒害作用主要表现在抑制水稻分蘖，使产量下降。土壤投加镍６０－２４０ｍｇ／ｋｇ条件下，可使水稻减产５％－２５％。水稻各器官镍累积量随土壤投加镍量的增加而增加，其分布规律为：根＞＞茎叶＞糙米，发现出明显的规律性。用０．００５ｍｏｌ／ＬＤＴＰＡ提取土壤中有效态镍能很好地体现水稻各器官对土壤镍的吸收关系。以减产１０％为依据，确定了在该土壤中镍的毒性临界值为１４３·１ｍｇ／ｋｇ（全量）和９．０ｍｇ／ｋｇ（０．００５ｍｏ１／ＬＤＴＰＡ提取量）  相似文献   

电刷镀镍基复合镀层性能综合研究     

艾薇  高志  潘红良  张菁菁  艾红 《长江大学学报》2005,2(1):45-47,i004

综述了近年来用电刷镀方法制备复合材料镀层的最新进展及研究动态.重点讨论了电刷镀镍基高硬度、耐磨复合镀层,高温抗腐蚀、高温耐磨复合镀层,自润滑复合镀层等的研究现状和发展趋势.并论述了这三种复合镀层的耐磨、耐蚀或高温抗腐蚀、高温耐磨等优点,最后对复合电刷镀技术的发展和应用前景进行了展望.  相似文献   

Effect of heavy metals on growth and survival of Macrophomina phaseolina (Tassi) Goid.     

R. C. Dubey  R. S. Dwivedi 《Biology and Fertility of Soils》1988,6(4):311-314

Summary The effects of Cd, Co, Ni and Zn on growth and survival of Macrophomina phaseolina were studied in vitro. Cd, Ni and Co at 500 g ml^–1 inhibited growth by 78.8%, 73.6% and 11.8%, respectively, after 4 days at 25 ± 1°C. The mycelial dry weight yield was enhanced by 2.1% at 100 g ml^–1 Zn. The population of the pathogen declined in soil amended separately with Cd, Co, Ni and Zn. Cd (4000 mg kg^–1) proved to be the most toxic, by completely inhibiting the survival of the pathogen in soil after 20 days.  相似文献   

Root morphology of Thlaspi goesingense Hálácsy grown on a serpentine soil     

Margarita L. Himmelbauer  Markus Puschenreiter  Andrea Schnepf  Willibald Loiskandl  Walter W. Wenzel 《植物养料与土壤学杂志》2005,168(1):138-144

The contribution of root morphology to enhanced uptake of heavy metals by hyperaccumulating plants is not well understood. The objective of this study was to describe root‐morphological characteristics of the natural nickel (Ni) hyperaccumulator Thlaspi goesingense Hálácsy. Plant samples were collected from a serpentine site near Redlschlag (East Austria), characterized by large soil Ni concentrations. Roots were evaluated for mass, length, surface area, diameter, and related ratios using an image‐analysis approach. Results showed that on the indigenous site, T. goesingense Hálácsy developed a fine‐branched root system, confined within a shallow soil depth. Coarse roots (>1 mm) accounted for about 60% of the total root mass (fresh and dry), while their contribution to the surface area and especially to the length of the system was small. Conversely, fine roots (<1 mm) represented 99% of the total root length and 88% of the surface area. The largest proportion of root length and area was found in the smallest diameter class of 0.0 to 0.5 mm. Shoot‐biomass production per unit root was high, in spite of the adverse soil conditions. Roots accounted for 8% of the total plant mass and about 4% of the total Ni accumulation. We conclude that the root system of natively grown T. goesingense Hálácsy exhibits a potential for enhanced Ni extraction from soil, since it mainly consists of very fine roots with extended absorptive area.  相似文献   

Design on the Welding Technology for 0Cr18Ni9 Production Separator     

XU Xian-guo~  LIU Shi-wei~  ZHOU Xing-yu~ 《保鲜与加工》2004,(6):21-24

According to the structural characters and the using properties of the big thick stainless steel chemical separator, a new welding process of the MAG welding is employed in this paper. The product's welding quality meets the design standard. The high productivity and profit are obtained with the new welding methods. The labor intensity of welding is reduced also.  相似文献   

干旱区绿洲土壤中Ni在芹菜中的富集迁移     

周婷  王胜利  南忠仁  赵翠翠  武文飞  廖琴 《干旱区资源与环境》2011,(12):171-176

以干旱区绿洲土壤为供试土壤,采用盆栽试验研究外源Ni污染对芹菜生长的影响及在芹菜中的富集迁移。结果表明：外源Ni的添加在低质量分数下能够促进芹菜的生长,达到一定临界值后,则出现明显的毒害作用;随着土壤中Ni的添加质量分数的增加,芹菜体内的Ni含量也随之增加。统计分析表明对芹菜根部和地上部吸收累积Ni贡献最大的均为铁锰氧...  相似文献   

SOC Definition of Battery Management System Used in Hybrid Vehicles     

HU Ming hui  QIN Da tong  SHU Hong  PU Bin 《保鲜与加工》2003,(4):20-23

For the requirement of building up the battery management system (BMS) of hybrid vehicles(HEVs), the real time measurement and estimation of charge (SOC) of the Ni MH batteries are investigated, because the popular SOC definition is unsuitable under the condition of variable current discharge. The origin of this kind unsuitability are analyzed ,and the characteristics and existing problems of different kinds of SOC measuring methods are compared. On the basis of mentioned above, some corrections are given to the popular SOC definition, and the new conception of SOC is proposed. The corrected definition of SOC is suitable for the real time SOC estimation under the variable current condition of the batteries used in HEVs. Based on the corrected SOC definition, the computational model of the new SOC is built and simulated. Satisfactory result has been obtained from comparisons of the simulating results, which shows that the theoretical model provides a basis for optimizing the adaptation of power system to the hybrid vehicles (HEVs).  相似文献   

PLANT AVAILABILITY OF NICKEL AS INFLUENCED BY FARMYARD MANURE AND ITS CRITICAL TOXIC LIMITS IN FRENCH BEAN     

Jyoti Pande  P. C. Srivastava  S. K. Singh 《Journal of plant nutrition》2013,36(3):384-395

A greenhouse experiment was conducted to evaluate the critical toxic limits of nickel (Ni) in french bean grown on a mollisol receiving varying levels of farmyard manure (FYM). Nickel levels beyond 10 mg kg^?1 soil brought a significant reduction in the dry matter yields of french bean. Application of FYM, especially at the higher level (4.46 g kg^?1 soil), had a favorable effect on the dry matter yields of french bean. The effect of FYM application was more pronounced at the medium levels of Ni. Application of higher level of FYM could decrease Ni concentration in plants significantly. Different soil extractants employed in the present study could be arranged in the following order of their Ni extraction power: Mehlich 3 > 0.1 N hydrochloric acid (HCl) > double acid > ammonium bicarbonate (AB)-diethylenetriaminepentaacetic acid (DTPA) > DTPA. Among different extractants evaluated in the study, DTPA (pH 7.3) appeared to be most promising for predicting Ni concentration in french bean. The threshold toxic levels of Ni in french bean and also in terms of extractable soil Ni contents were influenced by FYM application.  相似文献   

Increased plant nitrogen loss with increasing nitrogen applied in winter wheat observed with 15nitrogen     

H. L. Lees  W. R. Raun  G. V. Johnson 《Journal of plant nutrition》2013,36(2):219-230

Nitrogen (N) fertilizer is generally the most costly input for winter wheat (Triticum aestivum L.) production. Therefore, it was important to maximize fertilizer use efficiency and minimize N losses to the environment. One of the mechanisms responsible for decreased N use efficiency (NUE) was plant N loss. The objectives of this experiment were to determine fertilizer N recovery in winter wheat when produced for forage and grain, and to quantify potential plant N losses from flowering to maturity in winter wheat. Two long‐term (>25 years) winter wheat (Triticum aestivum L.) N rate fertility experiments (Experiment 222 and Experiment 502) were selected to evaluate ¹⁵N fertilizer recovery. Percent ¹⁵N recovery was determined from all microplots in plant tissue at flowering, in the grain, and straw at harvest and in the soil. Fertilizer N(¹⁵NH₄ ¹⁵NO₃) was applied atratesof 0, 45, 90, and 135kg N ha^‐1 in Experiment 222, and 0, 22, 45, 67, 90, and 112 kg N ha^‐1 in Experiment 502. The ratio ofNO₃ ^‐to NH₄ ⁺ in wheat forage at flowering was positively correlated with estimated plant N loss. Estimated plant N loss (total N uptake in wheat at flowering minus N uptake in the grain and straw at maturity) ranged from a net gain of 12 kg N ha^‐1 to a loss of 42 kg N ha^‐1, and losses increased with increasing N applied.  相似文献