侵染广东黄秋葵的木尔坦棉花曲叶病毒及伴随卫星DNA的分子特征   总被引：1，自引：0，他引：1  

董迪  朱艳华  何自福  柴兆祥  佘小漫  罗方芳 《华南农业大学学报》2012,33(1):33-39

从广东省表现黄脉曲叶的黄秋葵病株上分离到病毒分离物Okra06,PCR检测结果显示,该病毒属双生病毒科Geminiviridae菜豆金色花叶病毒属Begomovirus.基因克隆及序列分析结果表明,其基因组仅含A组分(DNA-A),全长为2 737 nt,推导编码6个开放阅读框(Open reading frame,ORF).该组分与木尔坦棉花曲叶病毒(Cotton leafcurl Multan virus,CLCuMV)分离物G6的核苷酸序列相似性最高,为99.7%;二者编码的6个ORF相似性分别为100%、100%、99.6%、99.8%、100%和99.7%.该病毒还伴随有卫星分子β(DNAβ),全长为1 346 nt,推导其互补链上编码1个ORF(C1).该分子与伴随CLCuMV分离物G6 DNAβ的核苷酸序列相似性也最高(99.5%).DNAβ系统进化分析显示,Okra06 DNAβ与CLCuMV-[G6]DNAβ、CLCuMV-[Gx08]DNAβ亲缘关系最近,三者形成一个独立分支;进一步与其他CLCuMV DNAβ和CLCuV DNAβ聚类在一起.这些结果证明,侵染广东黄秋葵的病毒分离物Okra06属于CLCuMV,且该分离物与引起广东朱槿曲叶病的CLCuMV-G6应同属木尔坦棉花曲叶病毒朱槿株系.  相似文献   

Response of okra plants to root and foliar applied boron.     

《Communications in Soil Science and Plant Analysis》2012,43(6):911-925

Abstract

The response of okra plants (Hibiscus esculentus) to root‐and foliar‐applied B at 0, 2 and 4 ppm was investigated in solution culture. Root‐B application higher than 2 ppm resulted in severe root burn and toxicity in the plants, whereas foliar‐B application up to 4 ppm produced adequate plant growth. Chlorophyll and carotene content of the leaves, flower number, stem diameter, plant height and dry matter production were drastically reduced at high root‐applied B when compared to the foliar treatments. Significant negative correlation between root‐applied B and plant height, stem diameter, leaf and flower number was established. Except for a significant negative correlation with leaf number, all other growth components were positively correlated with foliar application of B. At 2 ppm B, leaf‐B, ‐P, ‐K and ‐Ca were higher at the root‐B than at the foliar‐B treatments. Foliar application of B was superior to root‐application as was observed in the healthy growth of the okra plant.  相似文献   

The Measurement of Brown Leaf Spot on Rice     

M. O. Aluko 《国际虫害防治杂志》2013,59(1):76-81

Abstract

To obtain the potential range in disease development, a progressive increase in spore concentration of the imperfect state of Cochliobolus miyabeanus was used on twelve rice varieties differing in their resistance to brown leaf spot. An analysis of the relative contributory effect of increase in lesion size and numbers on the percentage leaf area diseased was used as a basis to develop a quantitative scoring method in chart form for brown spot disease. The chart was aided by the provision of diagrams of the different categories in disease intensity. The usefulness of this disease scoring chart and its limitations are discussed. Other methods of assessment are proposed to overcome the limitations especially in respect of varietal resistance and seedling blights.  相似文献   

黄秋葵作为禽用着色剂的品种比较试验     

叶花兰  刘东祥  刘国道 《勤云标准版测试》2007,(5)

通过14个作为禽用着色剂的黄秋葵品系比较试验,分析其种子发芽率、产量(叶片、茎杆、种子)、营养成分(叶片、茎杆)。结果表明:14个品系的种子发芽率差异极显著,其中021和007-1发芽率最高,达到92.5%;叶片和茎杆平均干物质产量差异均亦极显著,004-2叶片干物质产量最高,达到4948kg/hm2,018茎秆干物质产量最高,达到7103.4kg/hm2;008的营养价值最高;种子产量007-1最高,公顷产量大于3000kg。综合评价,018品系作为禽用着色剂较适合推广种植。  相似文献   

Plant character correlations and path analysis of pod yield in okra (Abelmoschus esculentus)     

O. J. Ariyo  M. E. Aken'ova  C. A. Fatokun 《Euphytica》1987,36(2):677-686

Summary Genotypic, phenotypic and environmental correlation coefficients were calculated for fifteen characters during two growing seasons. Correlation coefficients varie between seasons. Edible pod weight, edible pod length, edible pod width, number of seeds per plant, weight of 100 seeds, length of mature pods and number of branches per plant showed significant genotypic correlation with pod yield per plant; only number of branches per plant, edible pod length and weight of 100 seeds were phenotypically correlated with pod yield. Environmental correlation coefficients were generally low but edible pod length, final plant height and edible pod weight showed significant environmental correlation with pod yield during the two seasons.The genotypic correlation coefficients of selected eight characters with pod yield were partitioned into direct and indirect causes. In the early seasons, edible pod weight had the largest positive direct effect on pod yield with its largest indirect effect through reduction in edible pod width. Edible pod width which was highly correlated with pod yield had a negative direct effect on pod yield. In the late season, edible pod weight had the largest direct effect on pod yield, with large indirect effects through reduction in number of days to flowering and number of pods per plant. Number of days to flowering had a large direct effect on pod yield with its largest indirect effect through reduction in edible pod weight. The residual factors during the two seasons were negative. The study indicated that only number of branches per plant, edible pod length and weight of 100 seeds would be useful for indirect selection for pod yield. The path analysis indicated that edible pod weight was the most reliable and effective character to select for when high yield is the objective.  相似文献   

优质高产抗病黄秋葵新品种‘石秋葵2号’的选育     

宋聚红  王海山  相丛超  龚俊良  田东良 《中国瓜菜》2021,(1):80-83

‘石秋葵2号’是采用系统选育方法从‘12-5’与‘13-2’的杂交后经多代自交培育而成的黄秋葵新品种。石家庄地区4月下旬采用地膜覆盖露地种植,该品种生长整齐一致,植株节间短,平均株高1.67 m,茎粗4.0 cm,节间长3.0 cm,始花节位5~6节。叶片掌状5裂,绿色,叶面有硬毛,叶柄长36 cm,叶长22 cm,叶宽29 cm。花后4~6 d采摘最佳,嫩果五棱,果翠绿色且富有光泽,萼片不易脱落,果长10~12 cm,果肩1.5~1.7 cm,平均单果质量15.6 g。种子球形,灰绿色至褐色,表面被细毛,千粒重约68 g。生长势旺盛,抗病性强,丰产性好,生育期190 d,分批采摘,平均667 m^2产量2487.0 kg。适宜石家庄地区及生态条件相似的地区种植,2020年9月通过了河北省科技成果转化服务中心成果评价。  相似文献   

Emulsifying properties of three African food hydrocolloids: okra (Hibiscus esculentus), dika nut (Irvingia gabonensis), and khan (Belschmiedia sp.)     

Ndjouenkeu  R.  Akingbala  J. O.  Oguntimein  G. B. 《Plant foods for human nutrition (Dordrecht, Netherlands)》1997,51(3):245-255

This study investigated the emulsifying properties of okra (Hibiscus esculentus), dika nut (Irvingia gabonensis) and khan (Belschmiedia sp.), three African food hydrocolloids used to thicken and flavor soups. Results showed that khan has an emulsion potential approximately 20 and 100 times higher than the second and the first, respectively. A kinetic study indicated that the mechanism involved formation of thick and strong interfacial gum films around the oil globules, in addition to a high Water Absorption Capacity and weak gelling behavior of khan gum in solution. These results indicated that, when used in soups, which are typical oil/water emulsions, khan contribute both to thickening and stabilizing of the emulsion, whereas okra and dika nut functioned more as thickeners than as emulsion stabilizers.  相似文献   

Comparing weed and crop seedling response to pre-emergence pendimethalin application in Corchorus olitorius and Abelmoschus esculentus     

M.A.K. Smith   《Crop Protection》2006,25(12):1221-1226

Field experiments and bioassay tests were carried out to evaluate the relative response of the crops, tossa jute (Corchorus olitorius L.) and okra [Abelmoschus esculentus (L.) Moench], and their associated weeds to pre-emergence application of pendimethalin at 0.33, 0.66, 0.99, 1.32 kg ai ha⁻¹ and a pendimethalin+atrazine tank mixture at 1.32+2.05 kg ai ha⁻¹. Bioassay tests were carried out using herbicide solutions of corresponding concentrations and herbicide-treated soil. Field application of pendimethalin at 0.66 kg ai ha⁻¹ and the herbicide mixture in both crops effectively controlled most seedling weeds including Rottboellia cochinchinensis. Euphorbia heterophylla and Calopogonium mucunoides, which persisted from 2 weeks after treatment (WAT) were not controlled. Herbicide application during crop establishment markedly inhibited the growth of both seedling weeds and crops. The mixture caused the highest weed and crop injury. Pendimethalin at 0.33 kg ai ha⁻¹ had minimal effect on these crops. Weed growth, weed tolerance of herbicide treatment and crop seedling injury were higher in tossa jute than that in okra, under the conditions of this study. The use of low pendimethalin doses in an integrated weed management system will ensure effective control of seedling weeds, and prevent crop injury and residue accumulation in edible plant produce.  相似文献   

不同地区秋葵籽铅含量的测定     

刘超  景赞  吕雪梅  罗美琪 《中国果菜》2020,(4):37-39

重金属铅含量是评价秋葵籽质量的一项重要指标,为了考察不同地区秋葵籽中的铅含量,为制备秋葵籽油做准备,试验采集了5个不同地区的秋葵籽样本进行微波消解,采用电感耦合等离子体质谱仪测定了铅含量。结果表明,不同地区秋葵籽产品所检测出的铅含量有较大差异。5个检测样本中,铅含量最高的是云南昭通的,含量高达1.02 mg/kg;其次为浙江金华的,铅含量为0.157 mg/kg;其余3个样本中铅含量较少,分别为0.057、0.069、0.083 mg/kg。国家标准GB2762-2017中规定铅含量为0.2 mg/kg,只有云南昭通的秋葵籽中铅含量超标。可见秋葵生长受生态环境影响较明显,控制和降低秋葵产品中铅含量,选择无污染的土壤种植环境是必要的。  相似文献   

黔西北黄秋葵高效栽培技术   总被引：6，自引：1，他引：5  

赵维  何远宽  程娜  闵家媛  马杰 《农技服务》2010,27(8):1035-1036

黔西北地区属于高海拔冷凉性气候,通过对喜温性蔬菜黄秋葵的引进试种,逐步总结出黄秋葵的高产栽培技术,不仅丰富黔西北地区的农产品市场的蔬菜品种,同时取得了显著的经济效益、生态效益和社会效益。  相似文献