Molecular detection of important abortion-causing microorganisms in preputial swab of breeding bucks using PCR-based assays     

Chetna Gangwar  Gururaj Kumaresan  Anil Kumar Mishra  Anshuman Kumar  Anjali Pachoori  Sonia Saraswat  Narendra Pratap Singh  Suresh Dinkar Kharche 《Reproduction in domestic animals》2020,55(11):1520-1525

Infectious diseases and aetiological agents related to female reproductive systems were extensively covered compared to its male counterpart. There needs a proper study to bridge this gap, where microflora and infectious agents of both male and female reproductive are mutually intelligible. With this study, we aimed to evaluate the microbial contamination of the preputial cavity and also screened for abortion-causing agents which are zoonotic as well. In goats, such types of abortions are caused by Brucella melitensis, Chlamydophila, Campylobacter and Coxiella etc. One of the major sources of contamination of semen is the preputial cavity, which is exposed to the external environment leading to spread of infection into the female via semen straws or by natural service. In the current study, good quality bucks (n = 32, Barbari = 12, Jamunapari = 10, Jakhrana = 10) which were routinely used for semen collection were screened for their preputial swabs, for the presence of the above pathogens. For detection of Brucella melitensis, OMP31 based TaqMan® probe real-time PCR assay was used, and for Chlamydia, 16srRNA gene based SYBR® green real-time PCR assay was employed for detection of Chlamydophila abortus. While for Campylobacter spp. and Coxiella burnetii, 16srRNA gene based conventional PCR and Trans-PCR were used, respectively. In the current study, of the screened preputial swabs, none of them showed positive for Brucella and Coxiella, but of the screened 32 samples 17 showed positive for Chlamydia (53.13%) and two (6.25%) showed positive for Campylobacter spp. The current study emphasizes on the farms and laboratories which were regularly involved in screening of brucellosis also often overlook the other potential non-brucella pathogens, causing abortions eventually incurring severe economic losses to the goat keepers.  相似文献   

改良低聚果糖口服补液盐对兔腹泻的临床试验     

王钢 《安徽农业科学》2007,35(1):107-108

改良低聚果糖口服补液盐,使其具有调节微生态、补充VB1等作用,并应用于临床兔腹泻的防治.在腹泻预防试验中,改良低聚果糖口服补液盐、低聚果糖口服补液盐组和空白对照腹泻发病率分别为3.7%、12%、60%;在腹泻的治疗试验中,改良低聚果糖口服补液盐对腹泻症的治疗有效率达到89.1%;在腹泻和其他药物的治疗对照试验中,改良低聚果糖口服补液盐、低聚果糖口服补液盐组、黄连素组、空白对照,对腹泻症的治疗有效率分别为90.9%、78.9%、58.3%、18.8%.  相似文献   

Case Reports: Challenging Euthanasia Cases     

《Veterinary Clinics of North America: Small Animal Practice》2020,50(3):647-652

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Neoplastic melanocytic pleural effusion in a Portuguese water dog     

Erica Corda  Paulo Vilar Saavedra  Nora Berghoff  Rebecca C. Smedley  Jennifer S. Thomas 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2020,49(4):652-654

A 9-year-old castrated male Portuguese water dog was presented following incomplete excision of a malignant melanoma at the left lip commissure by the referring veterinarian. Physical examination was otherwise unremarkable. The patient was staged using thoracic radiographs, abdominal ultrasound, and fine-needle aspirates of the mandibular lymph nodes and spleen. Given the absence of any definitive evidence of metastasis, the malignant melanoma was surgically completely removed. The dog then received four melanoma vaccine doses as an adjuvant therapy and remained clinically healthy for more than 3 months after the last immunization. However, 232 days after the initial discovery of the lip mass, the dog was euthanized due to deterioration and a poor prognosis based on the presence of lung metastases and neoplastic melanocytic pleural effusion. The latter has been rarely reported in dogs, despite the high prevalence of oral malignant melanomas and the tendency of these tumors to metastasize to the lungs.  相似文献   

Investigation of the Effects of a Dietary Supplement on Insulin and Adipokine Concentrations in Equine Metabolic Syndrome/Insulin Dysregulation     

《Journal of Equine Veterinary Science》2020

High insulin concentrations are a common clinical feature of equine metabolic syndrome (EMS) and insulin dysregulation. Hyperinsulinemia can induce laminitis, so reduction of insulin concentrations in response to an oral challenge should decrease risk. In human studies, diets containing a polyphenol (resveratrol) led to improvements in insulin sensitivity. In rodents, the addition of leucine to a resveratrol supplement caused a decrease in the amount of resveratrol needed to achieve a clinical effect. We hypothesize a supplementation with a low dose of a synergistic polyphenol and amino acid blend including leucine (SPB+L) would improve metabolic health in EMS/insulin dysregulated horses. Fifteen EMS/ID horses received a high or low dose of SPB+ L daily for 6 weeks. Insulin during an oral sugar test (OST), body condition score, weight, baseline high-molecular-weight (HMW) adiponectin, triglycerides, nonesterified fatty acids, and tumor necrosis factor alpha were assessed before supplementation (PRE) and after supplementation (POST) via paired Student’s t-tests and a repeated-measures mixed-model analysis of variance (significant at P < .05). There were no differences between doses. Horses in the POST group weighed significantly less, had significantly higher baseline HMW adiponectin concentrations, and had significantly lower insulin concentrations at 60- and 75-minute time points (P < .05). Insulin concentrations of the horsesin the POST group, but not in the PRE group, were lower and similar to results from the study conducted three years before the present study (PRIOR) for 0- and 60-minute time points (P < .002). An increased HMW adiponectin level supports increasing insulin sensitivity after supplementation. These results suggest that SPB + L supplementation at either dose leads to improvements in the clinical manifestations of EMS/insulin dysregulation, potentially reducing laminitis risk.  相似文献   

离心泵平衡腔液体压力的计算与验证   总被引：2，自引：1，他引：1  

刘在伦  董玮  张楠  吴佼 《农业工程学报》2013,29(20):54-59

针对开平衡孔双密封环叶轮离心泵的平衡腔液体压力计算问题,基于液体通过叶轮平衡孔和后密封环间隙的泄漏量相等,引入了压力系数p和比面积k2个无因特征参数,推导出了平衡腔液体压力计算模型,其关系曲线为p= f (k )的无因次曲线,并给出了待定系数 a、b 的计算方法。在3BA-6泵上,取与计算相同的叶轮平衡孔直径进行了试验验证。结果表明,在泵设计工况下平衡腔液体压力的试验与理论无因次曲线较为一致,验证了平衡腔液体压力计算模型的正确性与可行性。该研究可为开平衡孔双密封环叶轮离心泵轴向力计算提供基础理论。  相似文献   

常年性变应性鼻炎手术疗法探讨   总被引：2，自引：0，他引：2  

周斌  陈向阳 《湛江医学院学报》2000,18(2):148-149

探索常年性变应性鼻炎的治疗新方法,方法选择各种非手术疗法不佳的常年性变应性鼻炎患者３６例为手术组（６例伴有支气管哮喘）,病程１．５－２０ａ局麻下行双侧蝶腭,筛前神经电灼加下鼻甲部切除切;另选３１例未行手术首作为对照组。结论蝶腭,筛前神经电灼加下鼻部分切除的新方法为治疗常年性变性鼻炎之有效的方法之一。  相似文献   

定向井螺杆泵抽油杆柱力学分析及防磨损措施   总被引：2，自引：0，他引：2  

扬也  张德实 《东北林业大学学报》2006,34(4):85-86

由于定向井井身结构特殊,利用螺杆泵进行抽油时如不采取任何措施势必造成杆柱严重磨损甚至卡死。为消除这种现象降低杆管磨损,提出在造斜点处的抽油杆柱上安装导向器以及在杆体上合理布置扶正器的技术措施。建立抽油杆柱有限元模型,利用ANSYS有限元软件进行分析计算,从而给出扶正器及导向器的合理配置原则。现场应用效果表明该方法实用、合理。  相似文献   

FAM3C activates Akt to promote viability of oral squamous-cell carcinoma cells     

CHI Yu-jing  LI Mei  YU Yi-meng  LIU Ai-yu  YU Wei-dong  MO Heng 《园艺学报》2019,35(3):424-429

AIM: To investigate the expression and roles of family with sequence similarity 3, member C (FAM3C) in oral squamous-cell carcinoma cells. METHODS: The mRNA and protein expression levels of FAM3C in dysplastic oral keratinocyte (DOK) and oral squamous-cell carcinoma WSU-HN6 cells were detected by RT-qPCR and Western blot. The WSU-HN6 cells were treated with siFAM3C or FAM3C antibody. After 24, 48 and 72 h, the viability of WSU-HN6 cells was measured by CCK-8 assay, and the activation of protein kinase B (Akt) was detected by Western blot. Adenovirus was used to mediate over-expression of FAM3C in the DOK cells. The DOK cell viability was measured by CCK-8 assay after adenovirus infection for 24, 48 and 72 h, and the activation of Akt was detected by Western blot. RESULTS: Compared with the DOK cells, the mRNA and protein levels of FAM3C were significantly increased in the WSU-HN6 cells (P<0.05). The viability of WSU-HN6 cells transfected with siFAM3C was significantly inhibited at 48 h and 72 h (P<0.05). siFAM3C treatment inhibited the activation of Akt (P<0.05). FAM3C antibody treatment also suppressed the viability of the WSU-HN6 cells at 48 h and 72 h and the activation of Akt (P<0.05). Over-expression of FAM3C in the DOK cells promoted the cell viability at 48 h and 72 h and activated Akt (P<0.05). CONCLUSION: FAM3C might promote oral squamous-cell carcinoma cell growth by activating Akt.  相似文献   

盐岩水平储气库的相似模拟建腔和长期稳定性分析     

刘建平  姜德义  陈结  刘新荣 《保鲜与加工》2017,(2):45-51

利用地下盐穴作为油气储库是一种常见的方法。但由于中国地下盐矿层状盐岩居多,特点是单层厚度薄,不溶物含量高,因此单井造腔腔型控制难度大,造腔速度慢。根据双井对流建库方法,通过实验模拟建造的水平盐穴为模型基础,依据湖北云应地区盐矿地质条件和盐岩的物理力学性质,数值模拟其在不同埋深情况下建造后腔体长期运行体积收缩情况。通过实验发现采用双井对流造腔方法建设储气库是可行的,腔体的形状可以通过流量和油液分界面来控制。对地下盐岩水平储气库的岩石长期蠕变模拟发现,水平储气库随着埋深越大相同蠕变期内条件下腔体的体积收缩率和腔体周边塑性变形区体积也越大,同时使用寿命也越短。  相似文献