家蚕微孢子虫(镇江株)β-微管蛋白基因部分片段的克隆及系统发育分析   总被引：2，自引：1，他引：1  

朱勃  沈中元  曹喜涛  徐莉  唐顺明 《蚕业科学》2006,32(4):507-511

设计1对正、反向引物btubf/btubr,对家蚕微孢子虫(镇江株)基因组DNA的β-微管蛋白(beta-tubulin)基因进行扩增,得到部分片段。经PCR鉴定、酶切及测序分析,该片段与Nosema属其它微孢子虫的beta-tubulin同源。采用邻近归并法(Neighbour-Joining)构建系统发育树,结果表明微孢子虫和真菌关系密切。研究结果对于微孢子虫的系统发育研究和家蚕微粒子病的治疗具有积极意义。  相似文献   

Viral encephalopathy and retinopathy of Dicentrarchus labrax and Sparus aurata farmed in Tunisia     

Chérif N  Thiéry R  Castric J  Biacchesi S  Brémont M  Thabti F  Limem L  Hammami S 《Veterinary research communications》2009,33(4):345-353

Viruses belonging to the Nodaviridae family cause disease worldwide among a large number of species of marine fish, and have been described in all continents. In the present study, a total of 69 farmed Tunisian sea bass (Dicentrarchus labrax) and 24 sea bream (Sparus aurata) samples were tested monthly for the detection of betanodavirus. The virus was identified in both species using indirect immunofluorescence assays (IFAT) and RT-PCR. In addition sequence analysis of part of the coat protein gene indicated that both species were infected by highly related, but distinct, strains belonging to the RGNNV genotype. The sequence of the coat protein gene of several strains was identical but up to 9 different sequences were detected in a single farm. In addition, viral sequences obtained from fish that were held at lower temperature (<20°C) were distinct from the rest of the sequences.  相似文献   

Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal   总被引：1，自引：0，他引：1  

Hisao Yoshikawa  Zhiliang Wu  Kishor Pandey  Basu Dev Pandey  Jeevan Bahadur Sherchand  Tetsuo Yanagi  Hiroji Kanbara 《Veterinary parasitology》2009,160(3-4):295-300

To investigate the possible transmission of Blastocystis organisms between local rhesus monkeys and children in Kathmandu, Nepal, we compared the subtype (ST) and sequence of Blastocystis isolates from children with gastrointestinal symptoms and local rhesus monkeys. Twenty and 10 Blastocystis isolates were established from 82 and 10 fecal samples obtained from children and monkeys, respectively. Subtype analysis with seven sequence-tagged site (STS) primers indicated that the prevalence of Blastocystis sp. ST1, ST2 and ST3 was 20%, 20% and 60% in the child isolates, respectively. In contrast to human isolates, ST3 was not found in monkey isolates and the prevalence of ST1 and ST2 was 50% and 70%, respectively, including three mixed STs1 and 2 and one isolate not amplified by any STS primers, respectively. Since Blastocystis sp. ST2 has been reported as the most dominant genotype in the survey of Blastocystis infection among the various monkey species, sequence comparison of the 150 bp variable region of the small subunit rRNA (SSU rRNA) gene was conducted among ST2 isolates of humans and monkeys. Sequence alignment of 24 clones developed from ST2 isolates of 4 humans and 4 monkeys showed three distinct subgroups, defined as ST2A, ST2B and ST2C. These three subgroups were shared between the child and monkey isolates. These results suggest that the local rhesus monkeys are a possible source of Blastocystis sp. ST2 infection of humans in Kathmandu.  相似文献   

Characterization of VP2 gene of an Indian Bluetongue virus serotype 2 and its close phylogenetic relationship to the Taiwan isolate     

Balumahendiran M  Sreenivasulu D  Kumar CA  Suryanarayana VV  Byregowda SM 《Research in veterinary science》2009,86(2):332-338

In this study we present the first report on partial amplification, sequencing and phylogenetic relationship of VP2 of the Indian isolate BTV-2. A PCR product of 1135 bp was amplified, cloned and sequenced. About 1063 bp of partial VP2 gene (1792-2854 bp region) of the Indian isolate was subjected to sequence analysis with already published sequences available in the genome database. The percent similarity of 85.2 was observed with Taiwan isolate and 59% with other isolates of BTV-2. However, 46.2% similarity with Australian BTV-1 and no significant similarity were noted with other serotypes. In-silico analysis and restriction enzyme digestion confirmed the presence of conserved SalI site at 2380 bp position in both Indian and Taiwan isolates. Phylogenetic analysis showed that all BTV-2 isolates formed one distinct group in which BTV-2 Indian and Taiwan isolate is more closely related and further demonstrated that BTV’s of the same serotype from different geographical regions were closely related at nucleotide and amino acid level, respectively.  相似文献   

马铃薯CBL9基因的基因组解析     

范华  马妍  孙凡  王琳  黄晓明 《山东农业科学》2012,44(8):10-12

利用比较基因组学的方法从马铃薯基因组中鉴定出一个与AtCBL9直系同源的基因StCBL9,并对该基因的结构、遗传进化、顺式元件进行了分析,为下一步研究该基因的功能和利用其进行马铃薯抗逆分子育种提供了有益借鉴。  相似文献   

微孢子虫系统发育研究进展     

马兰  李灿 《江西农业学报》2012,24(1):134-136,143

微孢子虫是一类专营寄生生活、具极丝但无线粒体的单细胞真核生物,介绍了生物学特征、核糖体的SSU rRNA、ITS间隔序列、LSU rRNA、微管蛋白基因及蛋白质组学在微孢子虫的系统发育研究中的应用。  相似文献   

基于线粒体控制区全序列的鹿亚科系统发育分析   总被引：1，自引：0，他引：1  

涂剑锋  邢秀梅  刘琳玲  鲁晓萍  杨福合 《西北农业学报》2012,21(3):22-26

测定13种鹿亚科动物线粒体DNA控制区全序列,并结合从GenBank获得的12种鹿亚科动物同源序列进行分析,进一步研究鹿亚科物种的分类和系统进化。结果显示,25种鹿亚科动物线粒体控制区序列全长为914～1 072bp,个体间序列差异为0.1%～12.2%,4个属间差异为8.0%～12.2%。构建的系统发育树结果表明,马鹿分为2个不同类群,麋鹿属的麋鹿、斑鹿属的豚鹿以及黇鹿属的黇鹿与鹿属的分化处于属间差异,支持将其并入鹿属的观点,坡鹿为鹿属中最原始的种。  相似文献   

熊蜂属系统进化的分子生物学研究进展(综述)     

黄家兴  吴杰  彭文君  安建东  国占宝  李继莲  童越敏 《中国蜂业》2005,56(8):7-9

近年来,分子生物学的迅速发展和广泛应用,使得熊蜂属系统进化研究方法发生重大的变化,系统进化的分子生物学研究方法正慢慢地向传统系统进化方法渗入.与传统的表型特征相比较,分子生物学方法受到环境的影响较少,因而更能反映出生物体在演变进化过程中的本质,其研究结果也更可靠.本文就目前国内外熊蜂属系统进化领域的分子生物学研究方法进行了综述,主要包括染色体计数法、蛋白质分析法、DNA测序法.  相似文献   

A new Badnavirus species detected in Bougainvillea in Brazil     

Eliana B. Rivas  Ligia M.L. Duarte  M. Amélia V. Alexandre  Flora M.C. Fernandes  Ricardo Harakava  César M. Chagas 《Journal of General Plant Pathology》2005,71(6):438-440

Badnavirus in Bougainvillea spectabilis showing virus-like symptoms was identified by the presence of bacilliform particles, measuring 125–130 × 30–40 nm in leaf-dip preparations and by analysis of its putative open reading frame 3 sequence. The virus, tentatively named Bougainvillea bacilliform virus (BBV), had the highest identities (up to 60%) with Spiraea yellow leaf spot virus, Gooseberry vein banding associated virus, Taro bacilliform virus, and Citrus yellow mosaic virus. In phylogenetic analysis, BBV clustered with Badnavirus putative species. Attempts to transmit the virus to several hosts failed. This is the first report of a new Badnavirus detected in Bougainvillea.  相似文献   

Characterization of a phytoplasma associated with witches' broom disease of potatoes in Korea     

Hee-Young Jung  Young-Il Hahm  Joon-Tak Lee  Tadaaki Hibi  Shigetou Namba 《Journal of General Plant Pathology》2003,69(1):87-89

 Symptoms of witches' broom disease caused by phytoplasma, including general stunting and yellowing, were observed in potatoes (Solanum tuberosum L.) in a storehouse on Jeju Island, Korea in 1998. Based on sequence analysis of DNA products from polymerase chain reaction (PCR)-amplified 16S ribosomal DNA and 16S–23S spacer region using universal phytoplasma primers, the phytoplasma associated with potato witches' broom disease (PWB) was identified as a member of 16S-group VIII. It was most closely related to elm AH phytoplasma (99.7% similarity, accession no. AF268895), which is in the clover proliferation (CP) subgroup. This report is the first from the East Asian continent of a plant pathogenic phytoplasma belonging to the CP subgroup and includes the nucleotide sequence of most of the potato phytoplasma 16S rDNA. Received: May 1, 2002 / Accepted: July 1, 2002  相似文献