甘肃党参中花生茎线虫的鉴定与记述     

倪春辉  李惠霞  刘永刚  韩变  石明明  魏雪娟  李文豪  徐雪芬 《华南农业大学学报》2022,43(4):99-105

【目的】明确甘肃省渭源县党参Codonopsis pilosula病样中的茎线虫种类。【方法】采用形态学特征、ITS-rDNA与28S-rDNA序列系统发育分析、特异性引物PCR扩增、PCR-ITS-RFLP相结合的方法进行种类鉴定。【结果】甘肃省党参茎线虫群体形态特征与花生茎线虫Ditylenchus arachis相似,形态测量均值虽存在差异,但是范围值基本一致;系统发育分析显示,该线虫与花生茎线虫聚为一支,特异性引物PCR扩增片段、PCR-ITS-RFLP图谱均与花生茎线虫相同。【结论】结合形态特征与分子特征分析,将甘肃党参茎线虫群体鉴定为花生茎线虫,表明该线虫已在甘肃发生分布。  相似文献   

萱草属植物的种质资源研究     

张少艾  李洁 《上海交通大学学报(农业科学版)》1995,(3)

本文对萱草属的３个野生种以及１６个园艺品种进行了全面相似性的聚类分析，并结合过氧化物同功酶谱，对萱草属的种质资源进行了初步的研究，并对桔红萱草ＨｅｍｅｒａｓｌｌｉｓａｕｒａｎｔｉａｃａＢａｋｅｒ的分类问题提出了看法，认为它归属于萱草Ｈ．ｆｕｌｖａＬ．比较妥当。  相似文献   

新疆野生油菜、野芥和黑芥的遗传分化及系统演化研究   总被引：4，自引：6，他引：4  

伍晓明  许鲲  王汉中  郑普英  陈碧云  宋运淳 《中国油料作物学报》2001,23(4):1-6

利用HPLC法对284份种子硫代葡萄糖甙组成分析表明，新疆野生油菜依据中特征硫代葡萄糖甙组成可划分为9类，91．2％的新疆野生油菜种子主要含羟基苄硫甙，8．8％的新疆野生油菜种子主要含丙烯基硫甙，与黑芥和芥菜型油菜特征硫代葡萄糖甙组成一致。RAPD分析将新疆野生油菜、野芥和黑芥划分为3簇，表明新疆野生油菜、野芥和黑芥之间存在明显的遗传距离，相对而言，新疆野生油菜与野芥间的遗传距离较近，与黑芥的遗传距离较远。新疆野生油菜可依据RAPD聚类分析结果划分为4簇，其中第1、2和4簇主要由来源于新疆塔城地区的新疆野生油菜组成，种子主要含丙烯基硫甙，第3簇为新疆巩留及其周边地区的新疆野生油菜,种子主要含羟基苄硫甙。研究为阐明我国芥菜型油菜起源进化途径提供了新的证据。  相似文献   

Highly polymorphic AFLP markers as a complementary tool to ITS sequences in assessing genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives     

Junchao Huang  Harold Corke  Mei Sun 《Genetic Resources and Crop Evolution》2002,49(6):541-550

Comparative analyses of genetic diversity and phylogenetic relationshipsof sweetpotato (Ipomoea batatas (L.) Lam.) and its wildrelatives in Ipomoea series Batataswere conducted using amplified fragment length polymorphism (AFLP) and sequencedata from the internal transcribed spacer (ITS) region of the ribosomal DNA. LowITS divergence among thirteen species of ser. Batatasresulted in poorly resolved relationships. More variable AFLP characters werefound to be more efficient in characterizing genetic diversity and phylogeneticrelationships at both intra- and interspecific levels within ser.Batatas. Highly informative AFLP fingerprints of 36accessions representing 10 species of ser. Batatas weregenerated using only six primer combinations. Of the species examined,I. trifida was found to be the mostclosely related to I. batatas, whileI. ramosissima andI. umbraticola were the most distantlyrelated to I. batatas. The highlypolymorphic AFLP markers are a valuable tool in assessing genetic diversity andphylogenetic relationships of sweetpotato and its wild relatives.  相似文献   

Microsatellite DNA Analysis of Wild Hops, Humulus lupulus L.     

Atsushi Murakami  Peter Darby  Branka Javornik  Maria S. S. Pais  Elisabeth Seigner  Anton Lutz  Petr Svoboda 《Genetic Resources and Crop Evolution》2006,53(8):1553-1562

To study the relationships and genetic diversity among wild hops, Humulus lupulus, we analyzed 133 samples of wild hops collected from Europe, Asia and North America using polymorphism on 11 microsatellite loci. Although only three primers showed bands in Japanese hops, all other samples showed polymorphic bands at most loci. There were no duplicate genotypes among samples of European, Chinese and North American hops, and each individual hop could be distinguished completely. The phylogenetic tree constructed from DA distance with the UPGMA method showed a large cluster comprised of European hops, although Russian hops from the Caucasus and Altai regions were separate from the European cluster. Chinese and North American samples gave distinct clusters suggesting genetic differentiation. This study has indicated that hop microsatellite DNA is differentiated, and is dependent upon the origin in regions of Europe, Asia and North America.  相似文献   

Phylogenetic characterisation of the G(L) sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark   总被引：3，自引：0，他引：3  

Larsen LE  Storgaard T  Holm E 《Veterinary microbiology》2001,80(4):339-346

  相似文献   

Genetic diversity and pathogenicity of <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> isolated from wilted Welsh onion in Japan     

Maha Laksha Mudiyanselage Chandrika Dissanayake  Rumi Kashima  Shuhei Tanaka  Shin-ichi Ito 《Journal of General Plant Pathology》2009,75(2):125-130

Thirty isolates of Fusarium oxysporum from wilted Welsh onion plants were examined for their diversity in nucleotide sequences of the ribosomal DNA (rDNA) intergenic spacer (IGS) region and for pathogenicity with regard to five Welsh onion cultivars. Phylogenetic analysis based on the IGS sequences revealed polyphyletic origins of the isolates and a relationship between phylogeny and pathogenicity; low virulence isolates differed genetically from those with high and moderate virulence. Mating type analysis revealed that all F. oxysporum isolates were MAT1-1 idiomorphs, suggesting that the pathogens may be clonal in the fields examined.  相似文献   

Genetic variation and mtCOI phylogeny for Bemisia tabaci (Hemiptera, Aleyrodidae) indicate that the ‘B’ biotype predominates in Iran     

H. Rajaei Shoorcheh  B. Kazemi  S. Manzari  J. K. Brown  A. Sarafrazi 《Journal of pest science》2008,81(4):199-206

Despite a large number of investigations on the molecular genetics and population structure of the whitefly Bemisia tabaci (Gennadius) complex, no such study had been conducted in Iran. The genetic variation of B. tabaci was examined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for 18 field collections from cucumber, eggplant, and tomato in four provinces of Iran. PCR amplification and restriction digestion with two enzymes detected 388 RFLP fragments, of which 16 fragments showed polymorphisms. Cluster analysis of these data placed all B. tabaci individuals within a single group, and there was no evidence for between- or within-population genetic variation. Phylogenetic (Clustal W) analysis of 42 B. tabaci mtCOI sequences (n = 21 field collections) from Iran, and a comparison with well-studied haplotype or biotype reference sequences available in public sequence databases, revealed that the Iranian B. tabaci populations were most closely related to the B biotype at 0–1.2% nucleotide identity. The B biotype is a well-known member of a sister clade from the Middle East–North African region of the world, owing to its nearly worldwide distribution and invasive characteristics. This report indicates that a single major haplotype of B biotype is prevalent in Iran and that its closest relative is the B biotype. Also, given the extent of known variation in the Middle East and African continent, data indicate somewhat surprisingly that the B. tabaci collections sampled in Iran had limited genetic variation and population substructure. Knowledge that the B biotype of B. tabaci predominates in Iran is important for designing effective pest management strategies given that biotypes of B. tabaci are known to differ greatly with respect to insecticide resistance, host range, virus–vector interactions, and other key biological characteristics.  相似文献   

基于线粒体DNA 4个基因/区域的矮小梅花鹿群体结构与起源进化分析     

王洪亮  郭肖兰  张然然  王磊  李婷  邢秀梅 《西北农业学报》2018,27(12):1723-1730

通过线粒体DNA上12S、ND5、Cytb和D-loop 4个基因或区域对分布于吉林省通化地区的矮小梅花鹿群体进行群体结构和起源进化分析,结果表明,各基因在群体中均包括3种单倍型,且每种单倍型个体组成相同。将4个基因整合后形成组合单倍型H1～H3,结合系统发育树判断,H1和H2单倍型与梅花鹿东北亚种聚为一类,而H3单倍型与梅花鹿四川亚种聚为一类,种群历史分析表明,该群体没有经历过扩张。因此认为,该矮小群体有3个母系起源,其中,H1和H2起源于东北亚种,H3可能起源于四川亚种,因为不确定四川亚种群体是否受到东北亚种影响。  相似文献   

玉米CBL基因的生物信息学分析   总被引：3，自引：0，他引：3  

李利斌  刘开昌  王殿峰  方志军  倪中福  张义荣 《玉米科学》2010,18(1):6-11

利用生物信息学方法从玉米的基因组中鉴定出10个CBL基因,并对这些基因的染色体分布、进化、蛋白基序、顺式反应元件进行了系统分析。结果表明,玉米的CBL基因分为3个不同的进化类群,在基因组中的分布是不均匀的,而且都含有响应逆境信号的不同顺式反应元件。玉米CBL基因预测编码的蛋白都含有3个EF-手型结构,而且被预测定位在不同的细胞组分中。说明它们可能参与玉米的逆境响应过程,而且在功能上各不相同。  相似文献