马氏珠母贝TNFR27基因克隆与功能初探     

梁碧丹  卢金昭  梁海鹰  张美珍  申铖皓  张彬 《水产学报》2024,48(6)

肿瘤坏死因子受体(tumor necrosis factor receptor,TNFR)是一类重要的细胞因子受体,主要参与细胞凋亡、宿主免疫防御、炎症等生物过程。本研究采用cDNA末端快速扩增(RACE)技术克隆了马氏珠母贝TNFR27(PmTNFR27)的cDNA全长并进行生物信息学分析,运用实时荧光定量PCR(qPCR)技术检测了PmTNFR27 mRNA在马氏珠母贝不同组织、脂多糖(LPS)、聚肌胞苷酸(Poly(I:C))及镉胁迫后的表达模式。结果显示：PmTNFR27 cDNA全长为1 524 bp,5′UTR长为186 bp,3′UTR长为248 bp,包含28 bp的poly(A)尾巴,开放阅读框(ORF)为1 062 bp,编码353个氨基酸;结构域预测表明PmTNFR27具有一个典型的CRD结构域和一个跨膜蛋白结构域,符合肿瘤坏死因子受体超家族特征;多序列比对结果表明贝类种间的相似性不高,但功能结构域位置较保守。系统进化树结果显示马氏珠母贝与其他贝类聚为一簇。qPCR结果显示,PmTNFR27 mRNA在马氏珠母贝各组织中均有表达,在鳃中表达量最高。LPS刺激后,PmTNFR27基因在鰓中的表达量于3 h达到最高,于72 h降到最低;Poly(I:C)刺激后,PmTNFR27基因在鰓中的表达量在6 h、12 h显著上升并达到最高至96 h时降到最低。镉胁迫后,3 h表达量达到最高,24 h、48 h表达量显著下降。研究结果显示PmTNFR27可能参与了马氏珠母贝的免疫应答反应。  相似文献   

日本珍珠贝人工繁殖苗在热带海区的养殖生长观察   总被引：1，自引：0，他引：1  

李有宁  吴开畅  喻达辉  陈明强  邢孔武  王雨 《南方水产》2006,2(3):50-53

日本珍珠贝引进海南陵水热带海区养殖,人工繁殖幼贝苗1·5~2·0mm下海养殖。经1年的养殖生长观察,结果为:平均壳长59·2mm、壳高59·7mm、壳宽20·7mm,平均个体重34·8g;养殖90d,生长速度较快,壳长平均增长值为11·9mm,相对增长率为55·4%;壳高平均增长值11·2mm,相对增长率61·2%;壳宽平均增长值5·9mm,相对增长率为190·3%。壳长、壳高和体重增长之间具有非常显著的相关关系。研究表明,该贝在海南热带海区养殖的生长速度快,是适宜引进的养殖对象。  相似文献   

合浦珠母贝选育基础群体的遗传多样性评价     

王婧璇  范嗣刚  黄桂菊  刘宝锁  张东玲  郭奕惠  喻达辉 《海洋渔业》2014,36(5)

利用微卫星标记对2012年3月和6月分别构建的两批共17个合浦珠母贝(Pinctada fucata)选育基础群体进行了遗传多样性分析。6对微卫星引物在17个群体共680个个体中检测到29个等位基因,每个位点的等位基因数在2~7之间,平均有效等位基因数在2.06~3.07之间,平均观测杂合度在0.31~0.58之间,平均期望杂合度在0.46~0.65之间,平均多态信息含量在0.39~0.58之间,高度多态(PIC≥0.50)群体有11个(占64.7%)。Hardy-Weinberg平衡检验(卡方检验)的结果显示,在检测得到的102个数据结果中,有53个(占52.0%)极显著地偏离了Hardy-Weinberg遗传平衡(P0.01)。从遗传偏离指数(D)的结果可以看出,17个群体在不同的微卫星位点存在不同程度的杂合子缺失现象。本研究表明,两批群体的遗传多样性参数非常接近,每个群体的遗传多样性都较高,对群体之间的遗传距离和遗传分化格局有了初步的把握,为下一步交配设计构建下一代核心群体奠定了理论基础。  相似文献   

Realized heritability and response to selection for shell height in the pearl oyster Pinctada fucata (Gould)   总被引：2，自引：0，他引：2  

Maoxian He  Yunyan Guan  Tao Yuan  & Hongyu Zhang 《Aquaculture Research》2008,39(8):801-805

The common pearl oyster, Pinctada fucata (Gould), is the most important species that is cultured for production of marine pearls in China. Heavy mortality and the decline of pearl quality have resulted in a breeding programme being established in recent years. In this study, we conducted selective breeding for the second generation of pearl oyster P. fucata (JCS-2) by mass selection for shell height (SH) with a selection intensity of 1.614, and analysed the growth of the selected line (JCS-2) and the non-selected control line (JCC) during a 1-year grow-out period. The results show that the selected line grew faster than the control one in the SH and total weight (TW) ( P <0.05), and there were higher proportion of larger sized oysters. Coefficient of variation for SH of JCS-2 was smaller than that of JCC. The current genetic gains and realized heritability for JCS-2 averaged 16.03 ± 4.79% and 0.713 ± 0.208 at 3–15 months of age respectively. The findings indicated the selection response to faster growth for SH is markedly effective in the second generation, and there was a high correlated response of TW when selecting for SH.  相似文献   

9个马氏珠母贝家系的中期生长性状评估   总被引：1，自引：0，他引：1  

汤健  刘文广  林坚士  何毛贤 《南方水产》2011,7(5):30-36

2008年3月以2006年构建的4个家系F1为亲本,采用不完全双列杂交法,成功构建了9个马氏珠母贝（Pinctadafacata）家系,在相同养殖条件下对其壳高、壳长、壳宽和体质量进行了周年测量,对不同遗传背景的家系和不同时期4个生长性状的生长速率进行了比较分析。结果显示,不同家系间及不同生长时期在各性状上存在显著差异（P〈0．05）。9个家系的壳高生长速率顺序为F11〉F4〉F9〉F3〉F6〉F12〉F2〉F7〉F5,壳长生长速率顺序为F11〉174〉F3〉F9〉F12〉F2〉F6〉F7〉F5,壳宽生长速率顺序为F6〉F11〉F3〉P9〉F4〉F12〉F2〉F5〉F7,体质量生长速率顺序为F11〉F12〉179〉F3〉F7〉F2〉F4〉F5〉F6。F11、附和F、9家系壳高生长较快,F11家系壳长生长较快,F11、F3和F9家系壳宽生长较快,F11、F12和F9家系体质量生长较快,彼此间差异显著（P〈0．05）。在不同的生长时期各生长指标差异显著性有所不同,10月～12月以壳高、壳长和壳宽增长较为显著,3月～5月以体质量增长较为显著;慢速生长期各生长性状指标增长速率差异均不显著,而快速增长时期各生长性状增长速率均存在显著性差异。筛选出生长性状最优的家系F11,为通过家系选择培育优良品种提供了遗传素材,同时为家系育种策略的制定提供了指导。  相似文献   

大珠母贝（Pinctada maxima）金属硫蛋白cDNA克隆与序列特征分析   总被引：2，自引：0，他引：2  

唐仁生  夏建红  王玉梅  喻达辉 《福建水产》2009,(1):31-36

金属硫蛋白（Metallothionein,MT）是一类普遍存在于生物体内、分子量低、半胱氨酸含量丰富、能够与二价重金属离子结合的蛋白质。MT在清除自由基、解除重金属毒性、参与体内微量元素代谢、防止细胞癌变等方面具有重要作用。本研究通过构建大珠母贝外套膜全长cDNA文库,首次克隆得到大珠母贝金属硫蛋白（PmMT）全长cDNA序列。该序列全长599bp,5’UTR（Untranslated Region）为75bp,3’UTR为296bp,开放阅读框（Open Reading Frame,ORF）为228bp,编码75个氨基酸。编码的氨基酸中半胱氨酸含量丰富,达到29．3％;赖氨酸和甘氨酸含量也较高,均为9．3％;不含苯丙氨酸、组氨酸、色氨酸、酪氨酸等芳香族氨基酸;含有软体动物等无脊椎动物金属硫蛋白的特征序列。序列特征分析表明,该序列具备金属硫蛋白的典型特征,是金属硫蛋白家族成员。  相似文献   

Effects of replacing microalgae with an artificial diet on pearl production traits and mineralization‐related gene expression in pearl oyster Pinctada fucata martensii          下载免费PDF全文

Chuang‐ye Yang  Qing‐heng Wang  Rui‐juan Hao  Yong‐shan Liao  Xiao‐dong Du  Yue‐wen Deng 《Aquaculture Research》2017,48(10):5331-5337

The effects of replacing dietary microalgae with artificial diet on pearl production traits and biomineralization‐related gene expression were investigated in pearl oyster (Pinctada fucata martensii). Three experiment groups (EG1, EG2 and EG3) were set. EG1 and EG3 were separately fed Platymonas subcordiformis and artificial diet (D4), respectively, and EG2 was fed with mixed P. subcordiformis and D4. A control group (CG) was cultured in natural sea. All groups were continuously fed for 150 days. The results showed that the survival rates of EG1, EG2 and EG3 were significantly higher than that of CG. No significant differences in retention rate and pearl thickness were observed among the four groups, but CG had the lowest values of both parameters. The relative expression levels of EGFR and FGF18 mRNA did not significantly differ among EG1, EG2 and EG3. Moreover, the relative expression levels of GHITM and TβR I mRNA significantly varied among the groups and EG3, and EG1 had the highest and lowest relative expression levels of GHITM and TβR I mRNA respectively. The relative expression levels of nacrein and chitin‐binding mRNA significantly differed among the groups, and EG1 had the lowest expression levels of nacrein and chitin‐binding mRNA. No significant differences in the relative expression levels of MSI60 and TIMP mRNA were observed among the groups. The results suggested that D4 can replace part of microalgae, which is helpful for further studies on developing artificial diet of pearl oyster.  相似文献   

马氏珠母贝不同地理种群内自繁和种群间杂交子一代形态性状参数及相关性分析   总被引：19，自引：0，他引：19  

王爱民  石耀华  周志刚 《海洋水产研究》2004,25(3):39-45

比较分析了马氏珠母贝不同地理种群内自繁产生的3个子一代群体(BB1、DD1、SS1)和不同地理种群间杂交产生的3个子一代群体(BD1、BS1、DS1)的11个形态性状、壳厚指数、壳重指数和肥满度。壳厚、壳重、总体重和绞合线深与壳长幂值之间的相关关系非常显著,壳宽和绞合线长与壳长对数值之间的相关关系非常显著。为解决我国海水珍珠养殖存在的主要问题,提出了我国马氏珠母贝选育的目标应该是提高生长速度和抗病力,兼顾提高珍珠的质量。壳长、总体重和壳重应该在马氏珠母贝选育过程中作为指示生长速度的主要形态性状指标。  相似文献   

Common pearl oysters in China, Japan, and Australia are conspecific: evidence from ITS sequences and AFLP     

Da Hui Yu  Xiaoping Jia  Ka Hou Chu 《Fisheries Science》2006,72(6):1183-1190

ABSTRACT:   To elucidate the species status of Pinctada fucata in China, P. fucata martensii in Japan and P. imbricata in Australia, one population of each taxon was studied using internal transcribed spacer 1 and 2 (ITS1, and ITS2) and amplified fragment length polymorphism (AFLP) markers. ITS1 and ITS2 were 401–405 and 229–237 bp long, respectively. Twenty-nine ITS1 and 15 ITS2 unique genotypes were obtained from 44 and 34 individuals, respectively, with some genotypes shared by two or three populations. In AFLP analysis, each individual exhibited a distinct phenotype. No population had diagnostic markers. Mean genetic divergences within and among the three populations were very low and overlapped (between-population: 0.7–0.9% for ITS1, 0.9–1.3% for ITS2, and 53.3–55.6% for AFLP; within-population: 0.5–0.9% for ITS1, 0.8–1.2% for ITS2, and 50.4–53.6% for AFLP). Low levels of genetic differentiation were observed among the three populations while the Australian population is partially genetically isolated. Under an infinite allele model, genetic differentiation among populations was not significant based on a permutation test. Under an infinite site model, most F _ST values were not significant for ITS data although they were significant for AFLP data. Network analysis using ITS data indicated that individuals from the same population did not cluster together. Analysis of molecular variance ( amova ) demonstrated that > 94% variation was contributed by within-population variation. These findings suggest that the three taxa are conspecific and Pinctada fucata is the correct name.  相似文献   

插核手术损伤对合浦珠母贝抗氧化免疫水平的影响     

张博  孟子豪  刘宝锁  李海梅  苏家齐  黄桂菊  吴开畅  喻达辉 《南方水产科学》2017,(5):72-77

分析了插核手术(未插入珠核及小片)之后合浦珠母贝(Pinctada fucata)闭壳肌、鳃以及外套膜中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性、总抗氧化能力(TAC)以及丙二醛(MDA)含量的变化,以探明合浦珠母贝抗氧化免疫系统对插核手术胁迫的响应特点。结果表明,各组织SOD、CAT活性和TAC基本上均呈现出下降-升高-再下降的波动变化。即大部分在第3小时达到最低水平而于第12小时达到或恢复到最高水平;相比之下,MDA含量整体上呈不断增高的趋势。相关性分析表明,MDA含量与TAC和CAT活性存在极显著的负相关性(P0.01);组织差异对SOD、CAT活性和TAC影响显著(P0.05),不同取样时间对SOD、CAT活性、TAC和MDA含量影响显著(P0.05)。研究结果显示插核手术损伤会造成合浦珠母贝抗氧化和免疫水平的波动,此过程中组织的氧化损伤程度与其抗氧化状态密切相关。  相似文献