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We hypothesized that inducing systemic resistances can contribute to the control of the nematode Rotylenchulus reniformis in pineapple. In greenhouse experiments conducted in Martinique, the pineapple cultivars Smooth Cayenne and MD-2 were treated with methyljasmonate (JAME) and salicylic acid (SA), elicitors of induced systemic resistance (ISR) and systemic acquired resistance (SAR). The efficacy of the elicitors was tested by inoculating plantlets grown in individual pots with a monospecific population of R. reniformis reared on Vigna unguiculata. The final nematode populations, 45 days after inoculation on MD-2 treated with JAME were reduced by 67.0% (p = 0.006). Nematode populations on MD-2 plants treated with SA were reduced by 55.8% (p = 0.016). Nematode populations on SC were not reduced by the elicitors. In a second experiment, using split-root systems, JAME was applied to MD-2 plantlets and enzymatic activities involved in plant defense and stress responses were monitored for 14 h after treatment. Additional pots were inoculated with nematodes 24 h after JAME treatment and examined ten days later. Transient stress was observed along with an increase in enzymatic response after inoculation with nematodes. These results showed that the MD-2 was primed through an ISR by JAME. The question now arises whether ISR can be specifically induced only in certain pineapple cultivars. Results are discussed from the perspective of introducing new strategies to manage pineapple nematodes. 相似文献
54.
采后低温诱导菠萝PPO活性升高的机理及其抑制途径 总被引:15,自引:0,他引:15
采后菠萝果实内的PPO(多酚氧化酶)活性和Pi(无机磷离子)浓度分布不均,二者密切相关。低温诱导显著提高了酸性磷酸酶的活性,使果实Pi明显升高。低温诱导减缓了磷脂酶D活性下降的速度,ABA处理可显著减缓低温对PPO的诱导,而CHI(环几亚酰胺)和乙醇也不同程度地抑制了PPO的升高,NaCl处理作用不明显 相似文献
55.
菠萝花粉母细胞减数分裂时的胞质分裂为连续型,四分孢子主要呈左右对称型或交叉型,侄生胚珠、双珠被,厚珠心,胚囊母细胞产生线状四分体。合点端一个大孢子发育,为功能大孢子。胚囊发育属蓼型。 相似文献
56.
菠萝茎蛋白酶的提取及其活性保护研究 总被引:5,自引:0,他引:5
本研究结果表明,菠萝茎组织中含有0.54~1.16%的蛋白酶.在酶膏的干燥过程中,45℃烘干使蛋白酶活性下降61.32%,而真空干燥的酶活性只下降18.32%.研究结果发现,硫代硫酸钠和半胱氨酸混用可部分地保护酶活性,使酶活性比对照提高67.37%.在提取中,用氯化钠、醋酸锌、乙二胺四乙酸和抗坏血酸溶液洗涤酶复合物可使酶活性提高110.48%.用0.15~0.25%的丹宁作酶的沉淀剂是适宜的,既可保证酶活性,也可保证有较高的酶产量. 相似文献
57.
采用单因素试验和正交试验方法对菠萝渣汁发酵果酒的工艺及有关工艺参数进行了初步探讨。结果表明:发酵温度、糖度、pH值、酵母接种量均为显著因素,最佳工艺条件为:主发酵温度28℃,糖度22%,pH值为4.0,酵母接种量为5%。 相似文献
58.
简述我国菠萝生产发展现状,并介绍了近年来福建省菠萝生产上使用的主要传统品种和引进品种的特征特性及种植表现. 相似文献
59.
Obeta J. A. N. Ugwuanyi J. O. 《Plant foods for human nutrition (Dordrecht, Netherlands)》1997,51(4):311-320
Growth of Neosartorya fischeri, N. fischeri var. spinosa and N. quadricincta on inoculated fruit juice agars (FJAs) consisting of 1% agar and juices from mango (MJA), orange (OJA) and pineapple (PJA) containing various levels of added sucrose was studied in terms of the average number of colonies produced by their ascospores, time (days) required before colonies were formed and developed to confluent mycelial mat as well as the level of reproductive spores produced. The means of the number of colonies on PJA and OJA without added sucrose were significantly (p =0.05) higher than that of MJA. Addition of sucrose at levels of 10% to PJA, 11.5% to OJA and 9% to MJA increased the numbers of colonies whereas sucrose levels of 30% or more in PJA, 31.5% or more in OJA and 29% or more in MJA decreased the number of colonies produced. At any level of added sucrose to FJAs, a significant (p =0.05) delay occurred before the colonies were formed and developed to confluent mycelial mat except in PJA containing 10% sucrose. All cultures produced abundant cleistothecia on all FJAs without added sucrose. Addition of sucrose at levels of 30% to PJA, 31.5% to OJA and 29% to MJA resulted in the production of abundant conidial heads with a corresponding decrease in the proportion of cleistothecia. 相似文献
60.
为了提高菠萝酒醪生产细菌纤维素的产量,运用Plackett Burman试验设计法对7个相关影响因素的效应进行评价,筛选出有显著效应的3个因素: MgSO4、FeSO4和无水乙醇,然后采用Box Behnken的中心组合试验设计和响应面分析方法(RSM)确定上述3个因素的最佳含量。经优化后的发酵培养基为:酵母膏7 g/L,K2HPO4 0.5 g/L,MgSO4 20 g/L,FeSO4 0.6 g/L,ZnSO4 3 g/L,柠檬酸0.3 g/L,无水乙醇9.4 mL/L。在此优化培养基条件下,菌株M438发酵生产细菌纤维素湿膜平均产量为376.8 g/L,是优化前的3倍。可见,Plackett Burman试验和响应面分析相结合的试验方法用于优化发酵培养基科学且有效。 相似文献