Phylogeny of Limenitidinae Butterflies （Lepidoptera： Nymphalidae） Inferred from Mitochondrial Cytochrome Oxidase I Gene Sequences     

ZHANG Min  CAO Tian-wen  ZHONG Yang  GUO Ya-ping  MA En-bo 《中国农业科学(英文版)》2011,10(4):566-575

The phylogenetic analyses of the subfamily Limenitidinae are performed based on 1471 bp of mtDNA cytochrome oxidase subunit I (C01) gene sequence data which were obtained from 21 individuals spanning 9 genera, along with those of 17species obtained from GenBank, using Apatura iris, Aglais urticae, and Polyura dolon as outgroup species. Although the transitions at the third codon positions of the COI data set were highly saturated, they were still retained for analysis as they contain the majority of the phylogenetic information, and thus, the maximum pasimony (MP) under different weighting schemes and maximum likelihood (ML) trees were reconstructed in this study. The results showed that within this subfamily, the results based on the COI gene sequences are approximately identical to the traditional classification results. However, the clustering of Lexias pardalis and Tanaecia julii within the genus Euthalia as well as the clustering of Phaedyma aspasia within the genus Neptis with weak support are different from that of the current classification scheme made by Chinese scholars. The genus Limenitis is splited into two subelusters in the trees constructed by using MP and ML methods. These results support one of the strongest hypotheses for the tribe relationships within Limenitidinae.  相似文献   

海藻酸钠包埋固定胆红素氧化酶的优化     

郑文爽  赵敏 《东北林业大学学报》2011,39(3):127-128

为改善胆红素氧化酶性质,提高酶的使用效率,以海藻酸钠为载体,采用包埋法固定了胆红素氧化酶,并优化了固定化条件,研究了固定化胆红素氧化酶的酶学性质.结果表明:海藻酸钠的质量浓度为0.10 g·mL-1、CaCl,浓度为0.20 mol·L-1、固定时间为1 h、酶的固定化效果最好,固定化酶的平均酶活力回收率达32%,固定...  相似文献   

槲皮素和硫脲对野桑蚕多酚氧化酶的抑制类型     

张永亮  朱勇 《湖北农业科学》2011,(21):4399-4400

多酚氧化酶(Polyphenol oxidase,PPO)在昆虫的变态发育和免疫防御中起着非常重要的作用,为开发以该酶为靶标的新型害虫抑制剂提供理论依据,以野桑蚕(Bombyx mandarina)五龄幼虫为材料提取多酚氧化酶,探讨了槲皮素和硫脲对该酶的抑制作用。结果表明,这两种抑制剂分别属于竞争性和非竞争性抑制剂。  相似文献   

钼对小鼠后代醛氧化酶和黄嘌呤氧化酶转录水平的影响     

张才  王雪莹  李鹏  白刃  陈庭锋  杨自军 《东北农业大学学报》2012,43(6):72-76

试验选用60只二月龄的清洁级ICR小鼠,随机分成4组,每组10只雌性和5只雄性小白鼠,雌雄分开饲养,饮水中加入不同剂量的钼,分别是空白对照组、低钼组(100 mg.L-1)、中钼组(200 mg.L-1)、高钼组(400mg.L-1),4周后雌雄合笼饲养。产仔后从每组仔鼠中随机雌性小白鼠30只和15只雄性小白鼠按原分组给予不同剂量的钼,8周后,再从每组中随机选取雌性小白鼠10只和5只雄性小白鼠合笼饲养,待其产仔。如此往复,使小鼠繁殖四代。以第三代(F2代)作为研究对象,应用实时荧光定量PCR的方法测定肝脏AOX和XO的转录水平。结果表明,与对照组相比F2代小鼠AOX和XO的转录水平随钼添加剂量的增加呈递增趋势,实验组与对照组差异显著(P<0.05)。可见长期钼暴露可提高小鼠后代AOX和XO的转录水平。  相似文献   

中华寿桃多酚氧化酶的特性研究   总被引：5，自引：0，他引：5  

段玉权  董维  张明晶  冯双庆  赵玉梅 《中国农业科学》2008,41(3):795-799

 【目的】对分离纯化后的中华寿桃果肉多酚氧化酶的酶学性质进行研究,探讨中华寿桃果肉褐变机理。【方法】以邻苯二酚为作用底物,用分光光度法在420 nm下测定不同pH、反应温度、底物浓度条件下桃果肉的多酚氧化酶（PPO）活性。【结果】中华寿桃果肉66 kD的PPO最适pH约为6.5,最适反应温度为60℃左右,该酶对温度有较强稳定性;该酶最适作用底物为绿原酸、儿茶酚和对羟基苯甲酸;FeSO4、EDTA和抗坏血酸等化合物可以抑制PPO活性,CaCl2、CuSO4和MnSO4等则起促进作用。【结论】该酶耐热能力强,适度低温、酸性条件、抗坏血酸处理等可以抑制该酶活性。  相似文献   

黑曲霉A9葡萄糖氧化酶的酶学性质研究   总被引：4，自引：1，他引：4  

王志新  于宏伟  韩军  李宁  林杨  贾英民 《河北农业大学学报》2006,29(4):69-72,83

为研究葡萄糖氧化酶的酶学性质,对黑曲霉A9发酵液提取纯化,酶蛋白达到电泳纯,然后对纯酶进行酶学性质研究。结果表明:葡萄糖氧化酶的分子量为138 200 Da,糖基化程度为2.67%,最适pH为6.7,最适温度为30℃,该酶的pH稳定范围较宽,而且热稳定性很好,Ag+、Hg2+、亚硫酸氢钠对酶有强烈的抑制作用,该酶对葡萄糖的Km值为35.74 mmol/L。该研究表明,黑曲霉葡萄糖氧化酶具有潜在的应用价值,最适合应用于食品、医药及生物等领域。  相似文献   

Response dynamics of three defense related enzymes in cotton leaves to the interactive stress of Helicoverpa armigera (Hübner) herbivory and omethoate application     

SHA Pin-jie  FAN Yin-jun  WANG Zhi-chao  SHI Xue-yan 《中国农业科学(英文版)》2015,(2):355-364

In order to explore the response dynamics of the activities of defense related enzymes in cotton leaves towards the interactive stress of Helicoverpa armigera herbivory and omethoate application, the activities of phenylalanine ammonia-lyase(PAL), lipoxygenase(LOX), and polyphenol oxidase(PPO) were examined from 6 to 126 h after cotton leaves were treated 12 h of H. armigera herbivory, and then sprayed with 800 mg L–1 omethoate. The results showed that the changes in the activities of PAL, LOX and PPO that occured under the interactive stress of H. armigera herbivory and omethoate application reflected the interactive effects of the two stresses on cotton defense. The similarity between the response dynamics of PAL, LOX, and PPO activities in cotton leaves under the interactive stress and that under H. armigera herbivory treatment alone showed that the induction of H. armigera herbivory on the activities of PAL, LOX and PPO in cotton leaves played a leading role in the interactive effects, and the effect of omethoate application played only a minor role. A joint factor analysis was performed according to a method which has been used to analyze the joint toxicity of pesticides; this analysis sought to clarify if there was a synergistic, antagonistic, or additive effect on PAL, LOX, and PPO activity in cotton leaves resulting from the interactive H. armigera herbivory and omethoate treatment. In the interactive effect on the response of PAL activity in cotton leaves, antagonistic effects of the omethoate application towards H. armigera herbivory were observed at 6 and 12 h. Synergistic effects were then observed at 18 and 30 h. Antagonistic effects were observed from 54 to 78 h and synergistic effects were finally observed at 126 h. The correlation between H. armigera herbivory and omethoate application in the interactive effect on cotton defense responses of LOX activity also fluctuated from synergism to antagonism during the time course. In the interactive effect on PPO activity, only antagonism was observed between H. armigera herbivory and omethoate application. In the interactive stress of H. armigera herbivory and omethoate application on cotton defense responses, omethoate affected the defense responses of cotton to H. armigera herbivory by producing antagonistic and synergistic effects. These results will be useful to understand the relationship between host plant and herbivorous pest.  相似文献   

榛属植物多酚提取方法的研究     

李修平  赵波  常瀚文  郑斌  李亚娟  张婷婷  杨鲲鹏  李秀霞 《安徽农业科学》2015,(21):282-285

植物多酚具有极强的抗氧化、抑菌、抗炎、防辐射、降血压等生理活性.为有效开发利用东北野生榛属资源,以东北野生平榛、毛榛为试验材料,以榛叶为提取对象,采用溶剂萃取法超声波辅助处理的提取方法,对2种榛属多酚进行提取分离,以确定榛属多酚提取分离最优方法.通过单因素试验及正交试验获得的最佳提取条件为:80％乙醇作为提取剂,料液比为1∶14 g/ml,75℃,浸提3h,浸提2次,超声波辅助40 min.在此条件下榛叶总多酚得率为2.565 6％.取材上,平榛与毛榛间差异不显著,但取材时间上差异显著,8月取材总多酚得率最高.  相似文献   

间伐对杉木人工林土壤多酚氧化酶活性的影响     

翟凯燕  陈信力  马婷瑶  关庆伟 《东北林业大学学报》2015,(7):88-91

以南京市溧水区林场间伐6 a后的25年生杉木人工林为对象,测定了弱度(30%)、中度(50%)、强度(70%)间伐及对照样地的土壤多酚氧化酶活性。结果表明:与对照相比,弱度间伐在秋季和冬季提高了土壤多酚氧化酶的活性,3种间伐强度在春季和夏季降低了多酚氧化酶活性;春夏季节土壤多酚氧化酶活性高,秋冬季节活性低,且前者与后者差异性显著(p0.05);土壤多酚氧化酶活性与土壤温度,全磷、全氮质量分数均呈极显著正相关(p0.01),与土壤含水量呈显著正相关(p0.05),与碳氮比呈显著负相关(p0.05),与土壤总有机碳质量分数、p H值相关性不显著。  相似文献   

龙井43号多酚氧化酶同工酶酶学性质研究     

张书芹  许雷  陈盛虎  徐小云  黄莹婕  姚燕妮  黄友谊 《湖北农业科学》2016,(6):1487-1491

以龙井43号[Camellia sinensis(L.)O.Ktze.cv.Longjing 43]鲜叶为材料,经分离纯化获得多酚氧化酶-同工酶(PPOⅡ),对其部分酶学性质进行了研究。结果表明,PPOⅡ最适反应pH为6.0;抑制剂抗坏血酸、亚硫酸钠和L-半胱氨酸对PPOⅡ的抑制作用随抑制剂浓度的增加而增强;Cu~(2+)浓度在0.25×10-7mol/L时,PPOⅡ活性最高;PPOⅡ的Km为13.05 mmol/L,v_(max)为0.019 OD_(460)/min。  相似文献