Pseudomonas anguilliseptica infection in farmed cod, Gadus morhua L   总被引：1，自引：0，他引：1  

Ferguson HW  Collins RO  Moore M  Coles M  MacPhee DD 《Journal of fish diseases》2004,27(4):249-253

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gacS基因在荧光假单胞菌FD6防治番茄灰霉病中的功能分析     

常 琳 肖 琦童蕴慧  徐敬友张清霞 《园艺学报》2014,41(4):681-685

 荧光假单胞菌（Pseudomonas fluorescens）FD6可产生多种抗菌物质,对多种植物病原真菌生长都有抑制作用。将FD6菌株感应激酶gacS基因部分保守区片段克隆到自杀载体中得到质粒pBS-S,将该质粒电击转化到FD6菌株中,利用同源重组构建了gacS基因插入突变体。经测定该突变体不产生抗生素硝吡咯菌素、2,4–二乙酰基间苯三酚、藤黄绿脓菌素、蛋白酶和氢氰酸,与野生型菌株相比形成生物膜和嗜铁素能力显著降低,并且丧失了对番茄灰霉病防病能力。gacS基因互补试验结果表明,gacS基因表达可恢复产生次生抗菌物质的能力,且对番茄灰霉病的防治效果与野生型菌株接近。由此证明,gacS基因是影响拮抗细菌FD6抗生素产生和对病害抑制能力的重要调控因子。  相似文献   

来源于云斑天牛肠道细菌Pseudomonas sp. TN06的β-折叠桶植酸酶基因phyA06的克隆、表达和酶学性质研究     

邵弨  郑长永  韩杏  张蕊  周峻沛  黄火清  杨培龙 《中国农业科技导报》2014,16(5):60-66

从云斑天牛肠道中筛选到一株产植酸酶活性较高的假单胞菌Pseudomonas sp.TN06。采用简并PCR和TAIL-PCR的方法获得一个新的β-折叠桶植酸酶基因(phyA06),开放阅读框全长1 902 bp,编码633个氨基酸和1个终止密码子,预测其前23个氨基酸为信号肽,含有两个β-折叠桶结构域。将phyA06基因在大肠杆菌中表达,重组蛋白经镍柱纯化后达到电泳纯。纯化后的重组酶最适pH为7.0,在pH 6.0~10.0的条件下具有很好的稳定性;最适温度为55℃,热稳定性良好。同时,该酶在低温下仍保持一定活力,甚至0℃仍有活性。该植酸酶具有应用于水产饲料行业的潜在价值。  相似文献   

三七细菌性叶斑病病原菌鉴定   总被引：1，自引：0，他引：1  

张照然  禹虹霞  马彦娇  张玉薇  周丽洪 《安徽农业科学》2014,(5):1351-1352,1424

[目的]对引起三七细菌性叶宽病的病原菌进行系统鉴定。[方法]以2011及2012年在云南文山及石林县的三七苗圃采集的三七细菌性叶斑病病株为材料,采用柯赫氏法则,检测分离菌株致病性并重新分离得到病原菌。通过病菌形态观察、致病性测定、16SrD—NA序列分析及生理生化特性分析（Biolog系统）对其进行鉴定。[结果]确定三七细菌性叶斑病病原菌为丁香假单胞菌丁香致病变种（Pseudomonas syringae pv．syringae）。[结论]该研究为三七细菌性叶瘫病菌详细鉴定的国内首报。  相似文献   

Expression of Toll-like receptor 2 and 4 in lymphocytes stimulated with N- (3- oxododecanoyl)- L- homoserine lactone     

LU Qi  YU Jia-lin  YANG Xi-qiang  LIU Wei 《园艺学报》2010,26(5):1003-1005

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兔绿脓假单胞菌性肺炎的诊断与防治     

孙善发  于燕妮  韦树丰 《中国动物检疫》1993,(4):25-27

本文报道近年来我省某试验养兔场兔群中流行一种生前以呼吸困难、口鼻出现血样分泌物症状,死后剖检以肺组织变性坏死为特征的疫情,经临床观察、实验诊断、病理剖检和病理组织学检查、流行病学调查及动物致病性实验研究,鉴定为绿脓假单胞菌(Ps.aeruginosa)引起的肺炎,该病特点传染快、死亡率高。通过流行病学调查,探索该病发病流行规律,为防治本病提供科学依据。  相似文献   

抗福美双假单胞菌株的NTG化学诱变及分子探针鉴定     

陈荣基  唐东阶  武波  柏学亮  马庆生 《广西农业生物科学》1996,(1)

利用微生物的抗药性筛选抗福美双的假单胞菌株Ｔ４６，然后通过亚硝基胍（ＮＴＧ）化学诱变获得了对福美双敏感的三株突变株Ｔ４６Ｎ１０、Ｔ４６Ｎ７和Ｔ４６Ｎ１７。利用这三个突变株作受体，通过基因克隆的方法筛选到了可使三个突变株恢复抗性的克隆，抗性基因分别被定位在７ｋｂ、２．５ｋｂ和２０ｋｂ的ＥｃｏＲⅠ片段上。将这三个克隆分别用（３２）Ｐ标记后制成分子探针，然后分别与三个突变菌株的总ＤＮＡ进行ＤＮＡ－ＤＮＡ分子杂交，结果表明，各突变株均存在与菌株Ｔ４６的抗福美双基因克隆的高度同源性，经结合形态、生理生化等分析后证实这三株突变株均源自于出发菌株Ｔ４６。  相似文献   

Influence of soil properties on the vertical movement of genetically-marked Pseudomonas fluorescens through large soil microcosms     

J. D. van Elsas  J. T. Trevors  L. S. van Overbeek 《Biology and Fertility of Soils》1991,10(4):249-255

Summary Vertical translocation of the introduced transposon Tn5-tagged Pseudomonas fluorescens cells was studies after irrigation of 50-cm long soil columns of loamy sand. The soil in the columns was slowly brought to saturation using groundwater, and enough water was then slowly added to permit collection of the percolated water. Introduced bacteria were transported to lower soil layers to a significantly higher degree in undisturbed soil cores than in repacked cores; water transport was hampered in both core types due to high soil bulk densities. Soil bulk density affected the degree of transport of the introduced cells; progressively more cells were translocated to deeper soil layers and into the percolation water at decreasing soil bulk densities. Repeated percolation of soil at a bulk density of 1.25 caused an increase in Tn5-tagged cell numbers in the lower soil layers and in the percolated water. Further, cells initially introduced into a dry (5.3% moisture) soil were translocated to a lesser extent than cells introduced into a wetter (13% moisture) soil. Finally, wheat roots enhanced the water-induced transport of introduced cells to the 40- and 50-cm deep soil layers and into the effluent, but not to the remaining soil layers. Large soil columns such as those used in the present study are useful in assessing the transport and survival of introduced bacterial cells in soils under a variety of simulated environmental conditions.  相似文献   

竹炭固定化紫外诱变假单胞菌处理间甲酚废水的研究     

周珊  虞方伯  郭明  单胜道  邸玉翠  徐少娟  李松华  郑圣丰 《林业科学》2011,47(9)

用紫外光对假单胞菌株进行诱变,以竹炭为载体,将紫外诱变假单胞菌固定在竹炭上,用竹炭固定化紫外诱变假单胞菌处理间甲酚水样。考察竹炭固定化紫外诱变假单胞菌投加量和水样pH值对间甲酚去除的影响以及进水浓度随反应时间的变化关系,研究竹炭固定化紫外诱变假单胞菌去除间甲酚的反应动力学。结果表明:相对于原菌株,菌株经紫外诱变后,生长周期缩短了6h。经紫外照射120s的假单胞菌可以在竹炭表面及内部孔隙形成明显菌胶团,诱变菌在竹炭上所成的生物量明显较未经诱变菌增加。竹炭固定化紫外诱变假单胞菌能有效地去除水样中间甲酚。竹炭固定化紫外诱变假单胞菌投加量和水样pH值影响到间甲酚的去除效果,pH值在4～6时,间甲酚的去除效果较好。20g竹炭固定化紫外诱变假单胞菌处理100mL初始浓度50,100,120,150,180mg·L-1间甲酚模拟水样42h,去除率依次为90.9%,76.4%,72.9%,64.6%和49.7%。竹炭固定化紫外诱变假单胞菌对间甲酚的去除能较好地符合零级反应方程。  相似文献   

Pine wilt disease: a short review of worldwide research   总被引：1，自引：0，他引：1  

Alexander Yu. RYSS  Oleg A. KULINICH  Jack R. SUTHERLAND 《中国林学(英文版)》2011,13(2):132-138

This article summarizes the results of the research papers presented at the International Symposium on pine wilt disease (IUFRO Working Party Meeting 4.04.03) held in July 2009, at Nanjing, China. The general topics covered were on pine wilt disease (PWD), its causal organism, the pinewood nematode (PWN) Bursaphelenchus xylophilus, plus other PWN-associated microorganisms that play a significant role in PWD such as bacteria (e.g. Pseudomonas fluorescens). Most of the papers that are reviewed are based on work on PWD-PWN in East Asia and Russia. Specific topics covered include: 1) the fundamental conceptions of PWD development, 2) pathogenicity, 3) host-parasite relationships including the histopathology of diseased conifers and the role of toxins from bacteria-nematode ecto-symbionts, 4) PWN life cycle and transmission, 5) B. xylophilus dissemination models, 6) associations (with other nematodes), 7) diagnostics, 8) quarantine and control of the PWN and 9) biocontrol of the PWN.  相似文献