不同处理对刺梨未受精胚珠愈伤组织诱导的影响   总被引：1，自引：0，他引：1  

陈红  张绿萍 《江西农业学报》2013,(6):49-50,54

以刺梨未受精胚珠为材料,研究了不同低温预处理时间、基本培养基、生长调节剂以及附加物对刺梨未受精胚珠愈伤组织形成的影响。结果表明:低温预处理3 d,刺梨未受精胚珠愈伤组织诱导率最高,为75.14%;以MS为基本培养基最有利于愈伤组织的诱导;6-BA 1.0 mg/L+NAA 1.0 mg/L为最优的生长调节剂组合,其愈伤组织诱导率高达84.58%;在培养基中添加100 mg/L的AgNO3有利于愈伤组织的形成,而添加Vc有利于愈伤组织转绿。  相似文献   

月季硬枝扦插技术   总被引：5，自引：3，他引：2  

吕研福 《北方园艺》2007,(5):137-138

应用2,4-D、IBA、NAA 3种激素分别处理月季插条,每种激素不同的浓度处理分别为25、50、100mg/L,以此比较不同种激素不同浓度对扦插的月季生根及根系生长的影响.结果表明:激素类IBA50mg/L为最适激素和最适浓度.  相似文献   

Dormancy and germination in Rosa multibracteata Hemsl. & E. H. Wilson     

Zhi-Qiong Zhou  Wei-Kai BaoNing Wu 《Scientia Horticulturae》2009

Most of the achenes produced by Rosa multibracteata Hemsl. & E. H Wilson are dormant on maturity and require pretreatment to stimulate germination. To investigate the mechanism of dormancy and to develop effective methods of improving germination, roles of the pericarp, testa, and embryo of R. multibracteata in regulating dormancy were studied by investigating the effect of different pretreatments on germination. The effects of temperature and water stress were also tested with achenes treated by warm plus cold stratification. In freshly harvested achenes, pericarps are permeable and the embryo fully developed, which eliminates the possibility of physical, morphological, or morphophysiological dormancy. Germination percentage remained low (<5%) despite softening the pericarp or even removing it fully. However, fully removing the testa improved germination significantly (39%), indicating the possible presence of germination inhibitors in the testa. Dry storage, scarification with sulphuric acid (H₂SO₄), and warm stratification proved ineffective by themselves but when combined with cold stratification, improved germination and shortened the cold stratification period needed to break dormancy. Dry storage for 68 weeks followed by cold stratification for 16 or 24 weeks resulted in maximum germination (72–79%) among all the treatments. In achenes scarified with H₂SO₄, germination increased with an increase in the duration of cold stratification. Neither gibberellic acid (GA₃) nor ‘smoke water’ (water through which smoke had been bubbled for 2 h) had any positive effect on germination even on seeds that had been mechanically scarified or stratified. Both high temperature and water stress lowered germination in achenes treated with warm plus cold stratification. Our results suggest that R. multibracteata achenes have an intermediate physiological dormancy, and that dry storage for 68 weeks followed by cold stratification for 16 or 24 weeks is the best method for propagating R. multibracteata from seed.  相似文献   

Resistance of 12 rose genotypes to 14 isolates of Diplocarpon rosae Wolf (rose blackspot) collected from eastern North America     

V. M. Whitaker    K. Zuzek    S. C. Hokanson 《Plant Breeding》2007,126(1):83-88

Blackspot disease, caused by the fungus Diplocarpon rosae Wolf, is the most serious disease problem of roses worldwide. From a breeding perspective, the documented occurrence of races in the pathosystem necessitates the characterization of the race structure for the pathogen, identification of resistance genes in rose germplasm, and determination of the nature of genetic resistance to breed stably resistant rose cultivars. The objective of this study was to characterize the diversity of eastern North American races of D. rosae and to simultaneously determine the degree to which partial resistances existed in rose hosts. Twelve rose genotypes were inoculated with 14 single‐conidial isolates collected from eastern North America. Three distinct D. rosae races were identified based on presence/absence of infection in the differential array. There were five distinct reaction patterns across pathogen isolates among the 12 rose genotypes. The partial resistance components of leaf area with symptoms, lesion length and incubation period were evaluated for all inoculated plants. Variation for expression of partial resistance was noted, and partial resistance appears to behave in a race‐specific manner for some rose genotypes. Early defoliation differed between genotypes and may prove to be another component of partial resistance.  相似文献   

The effects of onion and salt treatments on essential oil content and composition of Rosa damascena Mill     

Tayebeh Shamspur  Maryam MohamadiAli Mostafavi 《Industrial Crops and Products》2012,37(1):451-456

Due to the low oil content of Rosa damascena Mill., on one hand, and the wide spectrum of its application, on the other hand, it is desirable to find ways to increase the oil yield and also its quality. Therefore, in the present study the effects of chopped onion (1.0, 1.5, 2.0 and 4.0 g per 220 g of rose flower) and sodium chloride (11.0, 15.5, 22.0 and 30.0 g per 220 g of rose flower) which were added to rose flowers before distillation, were investigated on the quality and quantity of the oil. The quantity was measured as the ratio of oil to rose flower (w/w, %) and the quality was evaluated using the oil composition especially the content of monoterpene alcohols and hydrocarbons. Compared with corresponding control sample, treating with 1.5 g of chopped onion increased the oil content by 41% but it resulted in a lower quality; whereas in the presence of 1.0 g of onion, an increase of 30% and a decrease of 18% were observed in the percentage of monoterpene alcohols and hydrocarbons, respectively without any considerable change in the oil content. However, the effect of sodium chloride was somehow different from that of onion; such that in the optimal amount (22.0 g), the salt had no considerable effect on the quality but an increase of 21% in the oil yield.  相似文献   

黄蔷薇的组织培养     

张继东 《甘肃林业职业技术学院学报》2006,(1)

黄蔷薇采用不同发育期的当年生嫩枝,在无菌条件下处理后作为诱导培养的外植体,进行组织培养。经试验表明,适宜的诱导腋芽培养基为1/3MS BA0.3mg/L NAA0.1mg/L,继代培养基为1/2MS BA0.1mg/L IBAA0.2mg/L,生根培养基为1/2MS IAA0.5mg/L。试管苗在适宜的环境条件下培养后,可实现黄蔷薇的快速繁育。  相似文献   

花木嫩枝扦插技术的研究：[2]提高黄刺玫嫩枝扦插生根率的研究     

穆贵荣  张岐 《河北林果研究》1989,4(4):16-22

3年来的试验研究表明,黄刺玫在一般条件下,采取硬枝扦插不易成功。而6～7月选取嫩枝插穗进行植物生长调节剂处理,在塑料薄膜遮荫低棚条件下扦插,可以显著提高插穗生根率;若在间歇喷雾条件下,即使不使用植物生长调节剂处理,也可以取得比塑料薄膜遮荫低棚中更高些的插穗生根率。  相似文献   

激素及支持物对丰花月季快速繁殖的影响     

马纯艳  尤英豪  袁柄存 《吉林林业科技》2005,34(2):4-6

丰花月季的茎段(含腋芽)在MS 6-BA3．000 NAA0．400或MS 6-BA3.000 NAA0.005的培养基上，腋芽能顺利萌发，采用MS 6-BA3.000 NAA0．005可以从无菌苗诱导大量丛生芽，在继代培养中，不同含量的6-BA和NAA在诱导效率上有明显的不同。以沙子替代琼脂作为支持物，诱导初代腋芽的效率比琼脂高，幼苗的生根率和移栽成活率均很高。  相似文献   

不同基质和IBA浓度对野蔷薇及木香扦插生根的影响   总被引：2，自引：0，他引：2  

胡申明 《四川林业科技》2012,33(5):89-91,98

采用不同基质、不同IBA浓度处理野蔷薇、木香插穗,研究扦插基质、激素浓度对扦插生根的影响。结果表明野蔷薇在A2B2(100 mg·L-1IBA+蛭石)、木香在A3B1(200 mg·L-1IBA+珍珠岩)组合中成活率、生根数、平均根长最好。  相似文献   

基质和植物生长调节剂对白木香扦插生根的影响     

陶霞娟  孙丽芳  孙敬爽  余海  汤志敏 《安徽农业科学》2012,40(16):8991-8992

[目的]研究不同扦插基质和植物生长调节剂处理对白木香扦插生根的影响。[方法]以当年生健壮的白木香半木质化枝条为材料,分析不同处理对白木香扦插生根率、平均根数和平均根长的影响。[结果]以蛭石为扦插基质时,白木香的生根率、平均根数和平均根长最高,分别为78.3%、3.3条和3.6 cm;以清水处理为对照,用植物生长调节剂不同的种类和浓度处理时,以100 mg/L IBA的处理效果最佳,白木香的生根率、平均根数和平均根长分别为75.0%、6.2条和3.3 cm。[结论]白木香扦插繁殖以蛭石为扦插基质,用100mg/L IBA浸泡插穗2 h,可以获得较为理想的效果。  相似文献