月季花瓣衰老过程中可溶性蛋白的SDS—PAGE分析   总被引：8，自引：0，他引：8  

王然  罗松 《园艺学报》1998,25(3):306-307

对月季花瓣衰老过程中可溶性蛋白的ＳＤＳ－ＰＡＧＥ进行了分析，结果表明，蛋白条带变化表现出３种情况：（１）从瓶插开始到结束蛋白条带基本保持稳定；（２）随花瓣衰老，蛋白条带逐渐减弱、消失；（３）随着花的衰老，有新的蛋白条带出现。对花进行ＡＢＡ或乙烯促衰处理，也导致相应的某些蛋白条带的变化，说明这些蛋白的变化可能与衰老有关。  相似文献   

重组木复合刨花板截面最佳等强度的优化设计   总被引：1，自引：0，他引：1  

刘伟  马岩 《林业机械与木工设备》1997,25(11):15-17,14

以重组木复合刨花板微观力学的力泞 基础，应用材料力学的理论，以重组木复合刨菜板纵横向弹性模量与树种木材的差异最小为目标函数，并以重组木木束与刨花板刨花的相和学参数为优化变量，确定了重组木复合刨花板在满足最近似于实木性能条件下的最佳力学性能。本文所述的理论和方法为人造板复合材的优化设计提供了实用的理论和方法，也为人造板力学提供了基本理论和优化的方法。  相似文献   

一种制备(3R,4R)-4,7,7-三甲基-6-氧杂二环[3.2.1]辛烷-3,4-二醇的新方法     

黄道战  朱守记  蓝虹云  雷福厚 《林产化学与工业》2015,35(1):9-15

在催化剂过氧磷钨酸十二烷基吡啶盐(Cat-PW4)的作用下,α-蒎烯与H2O2反应生成主要产物(3R,4R)-4,7,7-三甲基-6-氧杂二环[3.2.1]辛烷-3,4-二醇。不同反应条件对反应转化率和选择性的实验结果表明,最佳反应条件为:12.8 mmolα-蒎烯、5 m L溶剂三氯甲烷、0.2 g催化剂、3.3 m L 30%H2O2,反应温度40℃,反应时间3 h,α-蒎烯转化率和产物的选择性分别为94.7%和39.8%。反应结束后,该产物存在于水相和有机相中,通过萃取和重结晶分离提纯,得率11%,纯度达到98%;其分子结构通过红外光谱、紫外光谱、1H核磁共振谱、13C核磁共振谱、低分辨率质谱及高分辨率质谱确证。  相似文献   

Hormonal influences on in vitro [35S]-sulfate uptake by gill arches from the goldfish (Carassius auratus L.)     

Tracy A. Marchant  Brenda M. Moroz 《Fish physiology and biochemistry》1993,11(1-6):393-399

A bioassay for insulin-like growth factor (IGF), based on the in vitro incorporation of [³⁵S]-sulfate into gill arch tissue was used to study the hormonal regulation of proteoglycan synthesis in the goldfish (Carassius auratus). [³⁵S]-sulfate incorporation into gill arch tissue was found to be time-dependent with maximal uptake occurring by 48h, suggesting that proteoglycan synthesis in this tissue was maintained for at least 48h in vitro. The addition of human recombinant IGF-I (IGF-I) to the incubation medium was found to significantly stimulate [³⁵S]-sulfate uptake into the gill arches, whereas bovine growth hormone (GH) was without effect. Porcine insulin was also stimulatory, but results indicate that the effects of porcine insulin and IGF-I may be mediated by a single receptor system. Finally, arches from hypophysectomized fish were significantly less responsive to IGF-I than were arches from sham-operated fish. Furthermore, administration of ovine GH in vivo appeared to increase subsequent responsiveness in vitro. Together, these results provide evidence that the growth-promoting actions of GH in the goldfish may be mediated, at least in part, by a peptide related in structure to mammalian IGF-I.  相似文献   

Effects of aromatase inhibitors on reproduction in male three-spined sticklebacks, Gasterosteus aculeatus, exposed to long and short photoperiods     

C. Bornestaf  E. Antonopoulou  I. Mayer  B. Borg 《Fish physiology and biochemistry》1997,16(5):419-423

Three-spined stickleback Gasterosteus aculeatus, males were implanted with Silastic capsules filled with different aromatase inhibitors; 1,4,6-androstatriene-3,17-dione or the non-steroidal CGS16949 A, 4-(5,6,7,8-tetrahydrimidazol [1,5-a]pyridin-5-yl) benzonitrile monohydrochloride or empty capsules. The fish were then exposed to long or short photoperiod. Under the long photoperiod most fish in all treatments displayed a hypertrophied kidney (a secondary sexual character in sticklebacks) and completed, quiescent spermatogenesis, similar as in the natural spawning period. Under the short photoperiod the controls had unstimulated kidneys and an active spermatogenesis, whereas the males implanted with both aromatase inhibitors had stimulated kidneys, though not to the extent as in the long photoperiod, and completed, quiescent spermatogenesis. These findings suggest that aromatization is of importance for the inhibitory effects of short photoperiod on reproduction in the stickleback. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

黄土丘陵区撂荒草地不同生态位物种非结构性碳水化合物对氮添加的响应     

郭建斌  徐红伟  薛萐 《水土保持学报》2022,36(3):153-158

为研究氮添加对不同生态位物种非结构性碳水化合物的影响。通过对黄土丘陵区典型撂荒草地进行3年的氮添加试验N0(0 g/(N·m²·a))、N3(3 g/(N·m²·a))、N6(6 g/(N·m²·a))和N9(9 g/(N·m²·a)),[JP]分析了不同生态位物种可溶性糖、淀粉和非结构性碳含量。结果表明:氮添加主要影响占据较高生态位的白羊草(Bothriochloa ischaemum)和长芒草(Stipa bungeana)的非结构性碳水化合物的储存,而对铁杆蒿(Artemisia sacrorum)和杂草影响较小。N6和N9处理对白羊草和长芒草非结构性碳、可溶性糖和淀粉的储存表现出明显的促进作用,这种作用在地上部分表现尤为明显。同时,植物相对重要值与植物地下非结构性碳和淀粉含量显著正相关,而与地上可溶性糖含量和可溶性糖/淀粉显著负相关。研究结果有利于加强对氮沉降背景下不同生态位物种碳储存及分配特征的认识。  相似文献   

铃铛果红枣复合饮料工艺技术研究     

李佳梅  于淼 《安徽农学通报》2014,(22)

以铃铛果为主要原料,研究了铃铛果和红枣复合饮料工艺,利用单因素试验和正交试验,对复合饮料的配方与工艺条件进行优化。结果表明：铃铛果汁和红枣汁1∶1情况下添加量为33%,蔗糖用量为9%,柠檬酸用量为0.05%,复合稳定剂的添加量为0.03%,115℃,灭菌5min,产品稳定性良好。  相似文献   

玫瑰红茶窨制技术研究   总被引：1，自引：0，他引：1  

王进  曹洪建  徐  谦等 《安徽农业科学》2014,(25):8749-8750

[目的]研究玫瑰红茶窨制的工艺技术。[方法]采下新鲜玫瑰花,利用玫瑰花瓣对成品红茶进行窨制,主要研究茶坯含水量、堆温、窨制时间和配花量4个因子对玫瑰红茶窨制效果的影响,优选最佳的窨制条件。[结果]研究表明,最适宜的玫瑰花窨制红茶技术条件为:茶坯含水量20%~30%,堆温30~35℃,窨制时间12 h左右,配花量60%~75%。[结论]研究可为玫瑰红茶的生产加工提供参考依据。  相似文献   

打叶复烤各工序对卷烟主流烟气氢氰酸与苯并[a]芘、巴豆醛释放量的影响研究     

陈昆燕 《湖南农业大学学报(自然科学版)》2014,40(2):144-147

选择红花大金元B3F、C3F、X2F 烟叶,对打叶复烤各工序卷烟主流烟气氢氰酸、苯并[a]芘和巴豆醛释放量进行研究。结果表明：各工序上部烟叶卷烟样品的HCN释放量最高,下部烟叶的最低,一润工序较处理前较大幅度提高了上部烟叶氢氰酸释放量;二润工序与一润工序相比,下部烟叶苯并[a]芘释放量降低9.36%;复烤工序与二润工序相比,下部烟叶巴豆醛释放量降低14.96%,中部烟叶苯并[a]芘释放量降低17.29%。综合结果,上部烟叶各工序间样品的主流烟气3种物质的变化强度都明显高于下部烟叶。  相似文献   

水保林体系的整体增益     

张淑芝  孙尚海 《中国水土保持》1995,(7):26-29

水保林体系的整体增益张淑芝，孙尚海（甘肃省平凉地区水保科研所，平凉市７４４６００）ＩｎｔｅｇｒａｌＢｅｎｅｆｉｔｏｆＦｏｒｅｓｔＳｙｓｔｅｍｆｏｒＳｏｌｌａｎｄＷａｔｅｒＣｏｎｓｅｒｖａｔｉｏｎ￥ＺｈａｎｇＳｈｕｚｈｉｅｔａｌ．（ＰｉｎｇｌｉａｎｇＳ...  相似文献