Simple sequence repeats as useful resources to study transcribed genes of cotton   总被引：12，自引：0，他引：12  

Sukumar Saha  Mehmet Karaca  Johnie N. Jenkins  Allan E. Zipf  O. Umesh K. Reddy  Ramesh V. Kantety 《Euphytica》2003,130(3):355-364

Microsatellites or Simple Sequence Repeats(SSRs) are informative molecular genetic markers in many crop species. SSRs are PCR-based, highly polymorphic, abundant, widely distributed throughout the genome and inherited in a co-dominant manner in most cases. Here we describe the presence of SSRs in cDNAs of cotton. Thirty one SSR primer pairs of 220 (∼14%) tested led to PCR amplification of discrete fragments using cotton leaf cDNA as template. Sequence analysis showed 25% of 24randomly selected cDNA clones amplified with different SSR primer pairs contained repeat motifs. We further showed that sequences from the SSR-containing cDNAs were conserved across G. barbadense and G. hirsutum, revealing the importance of the SSR markers for comparative mapping of transcribed genes. Data mining for plant SSR-ESTs from the publicly available databases identified SSRs motifs in many plant species,including cotton, in a range of 1.1 to4.8% of the submitted ESTs for a given species. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

利用已测序水稻品种分析其农艺性状基因座   总被引：1，自引：1，他引：1  

张启军  梁永书  叶少平  邓其明  王玲霞  李平  虞德容  邹江石  吕川根 《作物学报》2006,32(10):1503-1510

水稻重要农艺性状的基因定位研究在育种上具有重要意义。2004年在海南陵水县种植两个完全测序水稻品种日本晴与9311的F₂群体及双亲，分别考察了其单株分蘖数、穗数、有效分蘖数、穗长、主穗长、抽穗期、株高和剑叶8个农艺性状3次重复的平均值。用已构建的连锁遗传图谱（Nipponbare/9311-F₂遗传图谱）及Excel 2000和Mapmaker/QTL 1.1b软件对这8个性状间的相互关系和基因位点进行了分析。结果在LOD>2.0和P＜0.005的条件下共检测到41个QTLs，它们分布在水稻所有染色体上，单个QTL对性状表型贡献率11.0%～46.4%，其中大于20%的有22个。对选用已测序材料为亲本构建图谱来探讨水稻农艺性状的分子基础及其育种意义进行了讨论。  相似文献   

Assessment of genetic diversity within and among Basmati and non-Basmati rice varieties using AFLP,ISSR and SSR markers   总被引：6，自引：0，他引：6  

Navinder?Saini  Neelu?Jain  Sunita?Jain  Rajinder?K.?JainEmail author 《Euphytica》2004,140(3):133-146

Molecular markers provide novel tools to differentiate between the various grades of Basmati rice, maintain fair-trade practices and to determine its relationship with other rice groups in Oryza sativa. We have evaluated the genetic diversity and patterns of relationships among the 18 rice genotypes representative of the traditional Basmati, cross-bred Basmati and non-Basmati (indica and japonica) rice varieties using AFLP, ISSR and SSR markers. All the three marker systems generated higher levels of polymorphism and could distinguish between all the 18 rice cultivars. The minimum number of assay-units per system needed to distinguish between all the cultivars was one for AFLP, two for ISSR and five for SSR. A total of 171 (110 polymorphic), 240 (188 polymorphic) and 160 (159 polymorphic) bands were detected using five primer combinations of AFLP, 25 UBC ISSR primers and 30 well distributed, mapped SSR markers, respectively. The salient features of AFLP, ISSR and SSR marker data analyzed using clustering algorithms, principal component analysis, Mantel test and AMOVA analysis are as given below: (i) the two traditional Basmati rice varieties were genetically distinct from indica and japonica rice varieties and invariably formed a separate cluster, (ii) the six Basmati varieties developed from various indica × Basmati rice crosses and backcrosses were grouped variably depending upon the marker system employed; CSR30 and Super being more closer to traditional Basmati followed by HKR228, Kasturi, Pusa Basmati 1 and Sabarmati, (iii) AFLP, ISSR and SSR marker data-sets showed moderate levels of positive correlation (Mantel test, r = 0.42–0.50), and (iv) the partitioning of the variance among and within rice groups (traditional Basmati, cross-bred Basmati, indica and japonica) using AMOVA showed greater variation among than within groups using SSR data-set, while reverse was true for both ISSR and AFLP data-sets. The study emphasizes the need for using a combination of different marker systems for a comprehensive genetic analysis of Basmati rice germplasm. The high-level polymorphism generated by SSR, ISSR and AFLP assays described in this study shall provide novel markers to differentiate between traditional Basmati rice supplies from cheaper cross-bred Basmati and long-grain non-Basmati varieties at commercial level.The first two authors have equal contribution  相似文献   

Italian rice varieties: historical data, molecular markers and pedigrees to reveal their genetic relationships     

A. Spada    R. Mantegazza    M. Biloni    E. Caporali  F. Sala 《Plant Breeding》2004,123(2):105-111

Two molecular marker approaches [amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR)] were employed to study genomic relationship among 96 rice cultivars. These included most of the best reputed Italian accessions. AFLP produced 461 fragments, 248 (53%) of which were polymorphic, SSR produced four to 11 alleles in the 12 genomic loci investigated. Genomic similarity was estimated independently for the two molecular marker techniques. Both AFLP and SSR dendrograms agree in splitting the cultivars into two main clusters: a small one, comprising four exotic accessions, and a larger one which could be split into four subgroups. These were also analysed on the basis of historical and pedigree information. This is the first report on the application of DNA polymorphism analysis to reveal genomic relationship among cultivated Italian rice germplasm. Results will be useful for breeding programmes.  相似文献   

来自野生二粒小麦IW3和IW10的两个白粉病基因的鉴定及SSR标记定位     

李根桥  房体麟  张宏涛  解超杰  杨作民  孙其信  刘志勇 《作物学报》1963,35(5):761-767

野生二粒小麦(Triticum dicoccoides)是小麦抗病育种的重要资源库之一。来自以色列Mount Hermon的野生二粒小麦材料IW3 和IW10对我国小麦白粉病菌生理小种E09表现高抗。对硬粒小麦Langdon与IW3和IW10两个杂交组合F₂分离群体和F₃家系的遗传分析表明,IW3和IW10对小麦白粉菌E09的抗性均受显性单基因控制,暂被命名为MlIW3和MlIW10。采用BSA法和SSR标记分析,筛选到与抗白粉病基因MlIW3和MlIW10连锁的5个SSR标记,这两个基因均位于Xbarc84和Xwmc326之间,顺序为Xbarc84–4.6 cM–MlIW3–1.6 cM–Xwmc326和Xbarc84–6.6 cM–MlIW10–0.6 cM–Xwmc326。根据SSR分子标记的遗传图谱和在中国春的缺体—四体、双端体和缺失系的定位结果,这两个抗白粉病基因被定位在3BL染色体的末端。根据MlIW3和MlIW10的来源和分子标记定位结果,推断这两个基因可能是小麦抗白粉病基因Pm41或其等位基因或位于同一个基因簇中。  相似文献   

陆地棉耐盐性状与SSR分子标记的关联分析   总被引：1，自引：1，他引：1  

邵冰欣  王红梅  赵云雷  陈伟  郭志军  龚海燕  桑晓慧  叶武威  王俊娟 《棉花学报》2015,27(2):118-125

本研究以134份陆地棉栽培种为试验材料,测定其在0.3%盐浓度(质量分数)下的出苗率,并使用74对SSR引物对这些材料进行基因组变异扫描。利用Structure2.3.4软件分析该自然群体的遗传结构,在此基础上采用Tassel2.1软件对耐盐性状与SSR分子标记进行关联分析,寻找与棉花耐盐性状相关的分子标记。研究结果表明:(1)134份陆地棉栽培种的出苗率呈极显著差异,并筛选出27个盐敏感材料和10个耐盐材料。(2)74个SSR分子标记共检测出148个多态性位点,涉及246个等位变异,变异范围为2~7,平均每个标记3.32个;基因多样性指数变异范围为0.0295~0.4959,平均值为0.2897;SSR分子标记多态性信息含量(PIC)变幅为0.0290~0.3729,平均值为0.2381。(3)通过群体结构分析,将该自然群体划分2个亚群体,分别包含89份和45份材料。(4)关联分析共发现8个与棉花耐盐性状相关的SSR分子标记位点,表型变异解释率变幅为2.91%~7.82%,平均值为4.32%。此研究结果可以为棉花耐盐性状分子标记辅助选择育种提供参考。  相似文献   

用SSR标记分析豫综5号和金皇后综合种的遗传变异   总被引：1，自引：0，他引：1  

吴连成  侯本军  库丽霞  王铁固  孟庆雷  陈彦惠 《华北农学报》2007,22(3):30-34

利用40对SSR标记引物分析了豫综5号和金皇后综合种的遗传变异.结果表明:40对引物在两群体中都扩增出115个多态位点;累加40对引物扩增的基因型种类,豫综5号196种,金皇后194种;豫综5号的平均遗传距离为0.383 4,金皇后综合种为0.339 7;豫综5号的平均观察杂合度是0.382 6,平均期望杂合度是0.474 7;金皇后综合种的平均观察杂合度是0.329 2,平均期望杂合度是0.414 3.从以上结果来看,豫综5号的遗传变异较丰富.  相似文献   

小麦–华山新麦草异代换系DH2322的分子细胞遗传学鉴定     

王秀娟  陈新宏  庞玉辉  敬樊  张军  胡思远  昝凯  武军  杨群慧  赵继新 《作物学报》2015,41(2):207-213

利用分子标记、细胞学、基因组原位杂交(GISH)等技术,结合田间农艺性状调查,对小麦–华山新麦草七倍体材料H8911与硬粒小麦D4286杂交F4代分离群体中株系DH2322进行了综合鉴定。华山新麦草基因组特异SCAR标记鉴定表明,DH2322含有华山新麦草遗传物质;细胞遗传学观察显示,DH2322染色体构型为2n=42=21 II;有丝分裂和花粉母细胞减数分裂中期I基因组原位杂交(GISH)鉴定表明,DH2322的染色体由40条小麦染色体和2条华山新麦草Ns染色体构成,且2条Ns染色体能完全配对为一个二价体;SSR和STS分子标记分析表明,DH2322缺失小麦D基因组的2D染色体,而含有华山新麦草的2Ns染色体;农艺性状分析结果表明,DH2322具有双亲的形态学特征,结实性好,穗长和穗粒数显著大于亲本。  相似文献   

利用SSR标记分析栽培种花生多态性及亲缘关系   总被引：20，自引：0，他引：20  

韩柱强  高国庆  韦鹏霄  唐荣华  钟瑞春 《作物学报》2004,30(11):1097-1101

利用11对SSR引物对24个花生栽培品种（包括四大类型）进行PCR扩增分析，其中4对检测到明显的多态性，共检测到33个等位基因变异，每一个位点上检测到的等位变异数为5～13个，平均为8.25个。根据扩增结果可以将24个品种中的21个相互区分。供试品种间的遗传相似系数值在0.2～1.0之间，平均为0.4788。根据UPGMA聚类分析结果,供试  相似文献   

利用SSR标记分析小麦骨干亲本“矮孟牛”及衍生品种（系）的遗传多样性     

王珊珊李秀全  田纪春 《分子植物育种》2007,5(4):485-490

利用SSR标记研究了“矮孟牛”及其衍生品种（系）共计91份小麦材料的遗传多样性。20对SSR引物检测到120个多态性位点，每对引物多态性位点数平均为6．0个，变幅为3-9个。91份小麦材料遗传多样性GS变幅为0．411-0．961，遗传差异较大。UPGMA聚类分析将矮孟牛及其衍生品系分成四大类，能较好的反映品种（系）之间的遗传差异。矮孟牛亲本及矮孟牛七大类型一审定品种一衍生品系的遗传多样性指数变化呈逐渐上升趋势，但上升趋势逐渐减小，这与各育种单位反复利用矮孟牛及其衍生品种（系）有关；由矮孟牛与山农辐66、山农辐63为亲本杂交衍生出的材料较多，合理组配杂交亲本具有重要意义。  相似文献