Fitness costs of Vip3A resistance in Spodoptera frugiperda on different hosts     

Xuan Chen  Graham P Head  Paula Price  David L Kerns  Marlin E Rice  Fangneng Huang  Ryan T Gilreath  Fei Yang 《Pest management science》2019,75(4):1074-1080

  相似文献   

Effect of miRNA-33 on ABCA1/ABCG1 expression in RAW264.7 macrophage-derived foam cells after Hcy treatment     

DAI Pei  GAO Fen  GAO Hong-wei  WANG Yuan  FENG Gao-jie  ZHANG Qin-feng  BAI Rui  QIN Wei-wei  LI Hong  SONG Xiao-su 《园艺学报》2019,35(2):212-217

AIM:To study whether homocysteine (Hcy) inhibits the expression of ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) by microRNA-33 (miRNA-33) signaling, and reduces the efficiency of reverse cholesterol transport (RCT).METHODS:RAW264.7 macrophages were induced by oxidized low-density lipoprotein (ox-LDL) to establish foam cell model. Oil red O staining was used to determine whether the model was established successfully. miRNA-33 mimics and miRNA-33 inhibitor were transfected into the cells by Lipofectamine 2000, and the cells were exposed to Hcy at concentration of 5 mmol/L for 24 h. The intracellular lipid droplets were observed by Oil red O staining. The expression of ABCA1 and ABCG1 at mRNA and protein levels was determined by real-time PCR and Western blot. The cellular cholesterol content was analyzed by HPLC, and effluent rate of cholesterol was detected by the method of liquid scintillation counting.RESULTS:Compared with blank control group, the lipid content in miRNA-33 mimics group was increased, and the expression of ABCA1 and ABCG1 at mRNA and protein levels was decreased (P<0.05). The intracellular cholesterol content was increased gradually (P<0.05), and the cellular cholesterol efflux rate was gradually decreased (P<0.05) in miRNA-33 mimics group. Compared with blank control group, the testing results in miRNA-33 inhibitor group were the opposition of those in miRNA-33 mimics group (P<0.05). No diffe-rence of the above indexes among blank control group, miRNA-33 mimics-NC group and miRNA-33 inhibitor-NC group was observed.CONCLUSION:Hcy inhibits the mRNA and protein expression of ABCA1 and ABCG1 through miRNA-33 signaling, and reduces the efficiency of RCT in RAW264.7 macrophage-derived foam cells.  相似文献   

Tanshinone ⅡA induces apoptosis via JNK signaling pathway in human osteosarcoma HOS cells     

LU Jian-hong  HUANG Xiao-wen  JIN Yi-jun  YANG Yan-nan  WU Yi-zheng 《园艺学报》2019,35(1):28-33

AIM: To explore the effect of tanshinone ⅡA on human osteosarcoma HOS cells and the underlying mechanism.METHODS: The cell viability and the appropriate dose of tanshinone ⅡA were determined by CCK-8 assay. Colony formation assay and Transwell assay were used to investigate the proliferation and migration abilities of the HOS cells treated with tanshinone ⅡA. The apoptosis of the HOS cells was monitored by Hoechst 33258 staining, transmission electron microscopy and flow cytometry. The protein levels of apoptosis-related molecules and JNK signaling-associated proteins were determined by Western blot. Meanwhile, a JNK inhibitor was added for confirming the relationship between the pathway and apoptosis mentioned above.RESULTS: Tanshinone ⅡA inhibited both HOS cell proliferation and migration in a dose-and time-dependent manner. Exposure of the HOS cells to tanshinone ⅡA resulted in the activation of apoptosis. Tanshinone ⅡA treatment increased the protein levels of cleaved caspase-3, Bax and JNK signaling-associated proteins, and decreased the protein level of Bcl-2, which were reversed by JNK inhibitor SP600125. Moreover, the result of CCK-8 assay revealed that tanshinone ⅡA-induced cell death was alleviated by JNK inhibitor.CONCLUSION: Tanshinone ⅡA induces cell growth inhibition and the activation of apoptosis via JNK signaling pathway in human osteosarcoma HOS cells.  相似文献   

籼型优质三系香稻不育系广泰A的选育及应用     

柳武革  王丰  李金华  刘振荣  朱满山  廖亦龙  付崇允  刘迪林  曾学勤  马晓智 《杂交水稻》2021,(2):22-24

广泰A是广东省农业科学院水稻研究所以保持系中间材料荣丰B//米31/W254为母本,具有香味的保持系中间材料天丰B/粤丰B为父本进行杂交,然后通过分子标记辅助选择具有香味且直链淀粉较低的F3代株系与印尼水田谷胞质不育系荣丰A测交,后经连续回交转育而成的优质香稻不育系.该不育系具有败育彻底、育性稳定、柱头外露率高、异交习...  相似文献   

CPT1A基因在广西麻鸡的表达研究     

屠云洁  束婧婷  单艳菊  姬改革  章明  刘一帆  巨晓军  唐燕飞  蒋华莲  邹剑敏 《家畜生态学报》2021,42(2):14-18

该研究旨在阐明CPT1A基因在广西麻鸡组织表达和时序表达谱,为研究鸡体内脂肪代谢提供一定的基础资料.选取胚胎期到性成熟时的广西麻鸡,在不同发育时间点采集胸大肌、腓肠肌、心脏和肝脏组织样品,利用实时荧光定量方法,分析CPT1A基因在不同组织不同发育阶段表达规律.组织表达结果显示,CPT1A在广西麻鸡不同发育阶段4种组织中...  相似文献   

A型肉毒毒素Hc基因的原核表达、纯化及单抗的制备     

李秀山  刘选明  赵李剑  高姗  王景林 《动物医学进展》2007,28(2):8-11

在大肠埃希茵中高效表达的重组A型肉毒毒素保护性抗原,是以包涵体形式存在.溶解后的包涵体溶液经过处理,用镍柱亲和层析法纯化,得到纯度约为90%的融合蛋白.利用纯化的重组抗原BoNTa免疫Balb/c小鼠,获得分泌抗BoNTa特异性的3株杂交瘤细胞株2A2、4F7和2A8,其单抗鉴定均为IgG1亚型,滴度达到1:105.高纯度和活性单抗的获得为毒素检测试剂盒的研究奠定了基础.  相似文献   

早显性水稻三系不育系乐3A的选育     

周勇  黄世君  李乾安  魏应海  曾松  侯浪  郑英 《杂交水稻》2021,(2):28-29,78

乐3A是用IR超级稻与金23B杂交F3代的优良单株与矮败型不育系协青早A测交,并经多代回交转育而成的籼稻三系不育系.该不育系配合力强,异交习性好,且具有早熟不完全显性的特点.配制的杂交组合优质,早熟,抗倒性强.2012年8月,该不育系通过了四川省农作物品种审定委员会技术鉴定,所配组合乐3优727、乐3优2275于201...  相似文献   

BMY牛的选育进展     

亐开兴  赵刚  杨国荣  文际坤  袁希平  黄必志 《中国牛业科学》2007,33(3):18-21

总结了经历20多年选育的BMY牛所取得的进展,再通过3～5年的努力将完成BMY牛的培育,届时BMY牛将是一个适应我国南方炎热地区的热带肉牛新品种.  相似文献   

利用分子标记辅助选择技术改良三系不育系荃9311A稻瘟病抗性的研究     

张云虎  严志  杨力  李方宝  庞战士  邓一文  王慧  冯旗  张从合  陈琳  王合勤  黄艳玲  刘兴江  申广勒 《杂交水稻》2022,(1):19-24

为了提高三系不育系荃9311A的稻瘟病抗性,以含广谱稻瘟病抗性基因Pigm的水稻品种9311为供体,保持系荃9311B为受体和轮回亲本,通过分子标记辅助选择技术筛选抗性基因、剔除恢复基因和遗传背景筛选,在稻瘟病病区进行抗性鉴定,人工接种鉴定以及田间农艺性状考查,于BC2F2代获得有Pigm基因并剔除了恢复基因的改良株系,其后再与荃9311A杂交和连续回交,育成了农艺性状与荃9311A无显著差异而稻瘟病抗性明显增强的三系不育系K9311A及其保持系K9311B。  相似文献   

太行山前平原井灌农田点尺度土壤水分动态随机模拟   总被引：2，自引：0，他引：2  

任庆福  严登华  穆文彬  裴宏伟 《农业机械学报》2015,46(3):131-141

基于中国科学院栾城农业生态系统试验站2002—2008年夏玉米生长期内的土壤水分观测数据及2000—2008年的降水(灌溉)、气象、生物数据,结合Laio土壤水分动态随机模型研究了太行山山前平原典型农田点尺度土壤水分动态的随机性。结果表明:研究区2000—2008年夏玉米生长期内日平均降水量为10.71 mm,降水频率0.290 9,其中小雨、暴雨的发生频率表现出明显上升趋势,中雨的发生频率呈显著下降趋势,大雨发生频率表现出微弱下降倾向;玉米生长期的土壤含水率6月份处于增长期,7月份达到生长期最高值并稳定在32.2%的水平,8月份以后下降并在9月份趋于稳定;Laio模型模拟得到土壤相对湿度的概率密度函数在曲线形状(峰值、峰值出现的位置、90%置信区间)与数字特征(中位数、均值、方差)方面与观测结果一致(α=0.05),模型在井灌区具有很好的适用性,且可以将灌溉作为一次降雨事件来处理;应用Laio模型得到在多年平均降雨条件下,32.1 mm的田间净灌溉量可以在50%水平上使夏玉米生长期内的土壤含水率保持在田间持水量的80%以上。  相似文献