Induced out-of-season spawning of the mud crab, Scylla paramamosain (Estampador) and effects of temperature on embryo development     

Chaoshu Zeng 《Aquaculture Research》2007,38(14):1478-1485

Treated with combined bilateral eyestalk ablation and maintenance of water temperature at 22.5±1.5 °C, mud crab Scylla paramamosain females with mature ovaries were induced to produce eggs outside the natural spawning season in subtropical southern China. Newly extruded eggs from a crab were incubated in vitro at 10, 15, 20, 25, 27, 30, 35 °C, respectively, and the embryonic development was closely monitored. Abnormal cell division was observed at temperatures 10 and 35 °C. At 15 °C, development remained at the gastrula stage by day 32 post‐spawn, at which time the experiment was terminated. Hatching of in vitro incubated eggs occurred between 20 and 30 °C. An increase in incubation temperature from 20 to 25 °C reduced the incubation duration by 14 days, 2.6 times of that measured for a similar 5 °C increase from 25 to 30 °C. Embryonic development of S. paramamosain was divided into stage 0–10, and the duration of each stage was recorded for each incubation temperature. The information obtained allows accurate prediction of hatching time of female crabs incubated under variable temperatures. Larvae hatched from in vitro incubated eggs were reared to reach first juvenile crab stage and their dry weights were similar to those of larvae hatched naturally.  相似文献   

锯缘青蟹精子入卵过程的扫描电镜观察   总被引：5，自引：0，他引：5  

康现江 《水产学报》2000,24(5):403-406

利用扫描电镜详细观察了锯缘青蟹精子入卵的过程。精子以其核突起附着在卵膜上,并迅速发生顶体反应。顶体反应时,顶体囊外翻,顶体管前伸,精子核辐射臂收缩,并拖至顶体囊的后部。顶体管迅速穿过卵黄膜,携带核物质一同进入卵子。锯缘青蟹为多精着卵,数精入卵。本文同时探讨了精子顶体反应机制以及受精过程卵子的作用。  相似文献   

光照强度对拟穴青蟹幼体生长及能量收支的影响   总被引：1，自引：1，他引：1  

张胜负  蒋科技  顾孝连  乔振国 《海洋渔业》2011,33(2):187-194

以拟穴青蟹为研究对象,研究了1 000 lx、3 000 lx、5 000 lx、7 000 lx、9 000 lx五组光照强度对其溢状幼体蜕皮、生长及能量收支的影响.结果表明:(1)1 000 lx试验组幼体从第4期潘状幼体(Z<,4>)阶段起即期变态成活率明显下降,全部实验幼体最终均不能变态为大眼幼体(M);5 0...  相似文献   

温度对锯缘青蟹幼体存活与发育的影响   总被引：21，自引：1，他引：21  

曾朝曙  李少菁 《水产学报》1992,16(3):213-221

锯缘青蟹溞状幼体生长发育的适温范围为25—30℃。在该范围内,随着幼体发育,其生存最适温度逐渐上升。各期溞状幼体的发育均随温度的提高而加快,从孵化至第一次变态,30℃时平均发育时间比 25℃时短 6天。温度对溞状幼体的变态过程亦有明显影响,水温大于或达到 30℃时不利于变态的进行,但变态后的大眼幼体对高温适应力增强,35℃时仍可正常生长发育。不同批孵化的幼体在相同温度下存活与发育有明显差异。在对后期溞状幼体相对不适的 25℃下,溞状幼体发育期数变异的出现率较高。  相似文献   

Ontogeny of feeding apparatus and foregut of mud crab Scylla serrata Forsskål larvae     

Gil J Lumasag  Emilia T Quinitio  Riza O Aguilar  Rodolfo B Baldevarona  & Crispino A Saclauso 《Aquaculture Research》2007,38(14):1500-1511

The development of the feeding apparatus of the mud crab Scylla serrata larvae was studied using electron microscopy for mandibles and light microscopy for other paired mouthparts and the foregut. The six paired mouthparts, which consisted of the mandibles, maxillules, maxillae, first maxillipeds, second maxillipeds and third maxillipeds, were dissected from specimens representing each larval stage. The first five paired appendages were already present in newly hatched larvae while third maxillipeds appeared only at the megalopa stage. Mandibles displayed complex incisor and molar processes at early zoeal stages, which became simple in morphology at megalopa. Mandibular palp buds were observed at the zoea 5 stage and these became fully developed as three‐segmented mandibular palps at the megalopa stage. Endopods of other paired mouthparts exhibited increased number of setae and size as the individual metamorphosed from zoeal stages to megalopa and crab instar. The foregut appeared as a continuous cavity at zoea 1 where the cardiopyloric valve was indistinct while the filter gland was clearly identifiable. Zoea 2 and succeeding zoeal stages exhibited a setose foregut; the gastric mill and its lateral and median teeth were prominent at zoea 3 stage. The significance of these morphological changes is discussed in terms of its implication in larval feeding management.  相似文献   

恩诺沙星及其代谢产物环丙沙星在拟穴青蟹体内的药代动力学   总被引：1，自引：1，他引：1  

周帅  胡琳琳  房文红  周凯  于慧娟 《水产学报》2011,35(8):1182-1190

采用高效液相色谱法,研究盐度33条件下恩诺沙星口灌和肌肉注射给药(剂量10 mg/kg)后,恩诺沙星及其代谢物环丙沙星在拟穴青蟹体内的药代动力学和组织分布。血淋巴和组织中药代动力学参数采用基于统计矩原理的非房室模型进行计算。恩诺沙星口灌和肌肉注射拟穴青蟹给药后,血药达峰快,分别为0.5 h和1 min,达峰浓度分别为12.90和31.86 μg/mL,曲线下面积(AUC)分别为216.1和816.8 μg/(mL·h)。恩诺沙星在拟穴青蟹组织中分布较广,口灌给药下肌肉和肝胰腺AUC分别为445.9和817.6 μg/(g·h),肌肉注射给药下的AUC分别为554.7和2 573.7 μg/(g·h)。与其它水产动物相比,恩诺沙星在拟穴青蟹体内消除速度为中等水平,口灌和肌肉注射恩诺沙星后血药消除半衰期(t_1/2z)分别为26.45和57.02 h,总体清除率(CL_z)分别为0.054和0.012 L/(h·kg)。恩诺沙星在拟穴青蟹体内代谢生成环丙沙星的量较少,口灌给药下血淋巴、肌肉和肝胰腺的AUC_CIP/AUC_ENR分别为6.66%、3.66%和4.78%,肌肉注射给药下,其相应值分别为4.16%、7.24%和1.48%,在拟穴青蟹体内起药效作用仍是以恩诺沙星为主。以C_max/MIC、AUC_0-24/MIC评价恩诺沙星在青蟹体内的药效作用,建议给拟穴青蟹以10 mg/kg剂量每隔24小时投喂一次恩诺沙星,对弧菌引起的细菌性疾病具有较好的防治效果。  相似文献   

拟穴青蟹14-3-3基因全长cDNA的克隆及组织表达分析     

舒妙安  张龙韬  徐宾朋  胡杭娇  郭晓令 《水产学报》2012,36(8):1193-1200

采用RT-PCR及RACE技术,从拟穴青蟹眼柄组织中克隆获得14-3-3基因cDNA全序列.序列分析结果表明:拟穴青蟹14-3-3基因全长1112bp,开放阅读框长744 bp,编码由247个氨基酸组成的蛋白,分子量为28.086 ku,等电点为4.675.与其他物种14-3-3基因氨基酸序列进行同源性比较分析显示,拟穴青蟹14-3-3基因与斑节对虾14-3-3基因同源性最高(95％),依次为墨吉对虾(93％)、苜蓿切叶蜂(92％).聚类分析表明,拟穴青蟹14-3-3基因氨基酸序列与斑节对虾、墨吉对虾紧密聚为一支.经荧光定量检测,拟穴青蟹14-3-3基因在肝胰腺和肌肉中的表达量较高,其次为鳃、眼柄、心脏和肠,在胃中表达最少.盐度骤变实验结果表明:盐度胁迫24 h后,盐度的下降(5)或者上升(15、20、25、30)都引起了14-3-3基因在鳃中的表达量极显著上升(P＜0.01),盐度变化的幅度越大,14-3-3基因的表达量越多.实验结果为进一步深入研究14-3-3基因的功能及调控机理奠定基础.  相似文献   

低温驯化下锯缘青蟹肝胰腺的抗氧化效应及ATPase活性变化   总被引：1，自引：2，他引：1       下载免费PDF全文

孔祥会  王桂忠  李少菁  艾春香 《中国水产科学》2005,12(6):708-713

采用生物化学方法对低温驯化的锯缘青蟹（Scylla serrata）肝胰腺中抗氧化酶（SOD、CAT和GPX）活性和脂类过氧化产物MDA含量以及Na^＋-K^＋ -ATPase、Mg^2＋ -ATPase、Ca^2＋ -ATPase和Ca^2＋ -Mg^2＋ -ATPase活性进行测定。结果显示，锯缘青蟹肝胰腺中抗氧化酶（SOD、CAT和GPX）活性随驯化温度的降低而降低；而MDA含量则是随驯化温度的降低而升高，其变化正好与抗氧化酶活性相反。Na^2＋ -K^＋ -ATPase、Mg^2＋ -ATPase和Ca^2＋ -Mg^2＋ -ATPase活性均是随驯化温度的降低而升高；而Ca^2＋ -ATPase活性没有表现出这一明显变化趋势，但低温驯化下仍然是酶活性升高。锯缘青蟹低温驯化下抗氧化酶活性降低表明其清除活性氧能力下降，从而导致MDA在肝胰腺中积累。ATPase活性低温驯化下升高，说明其表现出明显的温度补偿效应（低温使酶活性下降，同时又诱导酶合成增加，从而使酶浓度增加，其总的酶活性仍能维持机体正常的生理功能），但各种ATPase活性的温度补偿存在差异。  相似文献   

锯缘青蟹蜕皮抑制激素cDNA的分子克隆及其表达分析   总被引：5，自引：1，他引：5  

邱高峰 《水产学报》2003,27(3):207-212

根据近缘种类同源序列设计简并引物,采用逆转录聚合酶链式反应(RT—PCR)技术,首次扩增获得锯缘青蟹(Scylla serrata)眼柄组织中编码蜕皮抑制激素(MIH)成熟肽全长cDNA序列及部分信号肽序列。将该序列克隆到pUCm—T载体上进行序列测定。结果表明,编码锯缘青蟹MIH成熟肽的cDNA由234个碱基组成,由此推测MIH成熟肽含78个氨基酸残基。该序列不仅与其它短尾类甲壳动物的MIH氨基酸序列具有高度的同源性(79％～9l％),还与该激素同一家族的性腺抑制激素、大颚器抑制激素的氨基酸序列具有较高的相似性。RNA斑点杂交结果显示,MIH基因在眼柄神经节及脑组织中均有表达,而在肌肉、中肠腺中不表达。  相似文献   

Overwintering behaviour affects distinctive flavour of mud crab Scylla paramamosain in commercial farms     

Lei Tang  Huan Wang  Hongling Wei  Chunyu Ye  Lizhi Chen  Hongzhi Yao  Ce Shi  Changkao Mu  Chunlin Wang 《Aquaculture Research》2020,51(1):29-40

In order to reveal the influence of overwintering behaviour on the flavour of Scylla paramamosain, this study selected crabs of before and after overwintering in SM (Sanmen County, Zhejiang Province), HP (Hepu County, Guangxi Province) and WN (Wanning city, Hainan Province). The contents of flavouring‐related substances in hepatopancreas, muscles and gonads were determined by high‐performance liquid chromatography (HPLC); taste activity value (TAV) and equivalent umami concentration (EUC) were calculated. The results showed that sweetish amino acids were mainly accumulated in muscles, umami amino acids were mainly accumulated in hepatopancreas, free nucleotides were mainly accumulated in gonads, and the EUC value in gonads was the highest. After overwintering, the content of sweetish, umami amino acids and free nucleotides in muscle and hepatopancreas decreased significantly, while the content of flavour‐tasting amino acids in gonads did not change significantly, but the content of umami nucleotides increased significantly. Therefore, overwintering behaviour decreased the flavour of muscle and hepatopancreas, slightly enhanced gonad and the overall flavour of S. paramamosain before overwintering was better than that after overwintering.  相似文献