UV-B辐射对两种海洋微藻生长的影响   总被引：13，自引：2，他引：11  

刘泳  王悠  唐学玺  李永祺  刘文举 《海洋水产研究》2000,21(2):22-26

研究了两种微藻－－小新月菱形与青岛大扁藻在一范围内（小新月菱形藻０～３．００ｊ／ｍ＾２,青岛大扁藻０～１１．２５Ｊ／ｍ＾２）ＵＶ－Ｂ辐射对其相对增长率（Ｋ）,蛋白质含量、类胡萝卜素含量以及超氧化物歧化酶（Ｓｕｐｅｒｏｘｉｄｅ ｄｉｓｍｕｔａｓｃ,ＳＯＤ）活性的影响。结果表明,（１）随着辐射剂量的升高,两种微藻的相对增长率（Ｋ）逐渐下降,其各自的半数有效抑制浓度４８ｈ,ＥＣ５０分别为,小新月菱形藻１  相似文献   

微藻空气净化系统     

李静  卢彦兴  王琳  刘敏胜 《河北渔业》2016,(5)

该技术是利用微藻光合作用,将二氧化碳转化为氧气对空气进行净化。本文介绍的是以板式光生物反应器为依托的微藻光合作用系统,该系统制成隔断水晶墙适合室内应用,水晶墙式光生物反应器相当于一面"会呼吸的墙",起到了室内空气净化的效果。  相似文献   

微藻对海水中重金属离子的净化作用研究     

张建民  王相伟  赵宏  梁奉军 《延边大学农学学报》2007,29(3):204-207

探讨了海洋微藻(塔胞藻)对重金属离子的净化作用.在其培养液中加入含有Pb、Hg、Cr、Cd等离子的化合物,塔胞藻经过一定时间生长后调查培养液内重金属离子的含量.结果表明,液体内藻细胞密度达到104个/mL,对重金属离子的净化作用最明显,而且微藻对不同重金属离子的净化效率也各不相同.  相似文献   

毛蚶对单胞藻的滤除率、选择性和消化状况     

沈和定  张饮江  卢华春  周培根  吴建中 《上海海洋大学学报》2006,(2):195-200

测定了不同水温下毛蚶对5种单胞藻的滤除率、选择性和表观消化状况,结果表明,在16℃~24℃的水温范围内,毛蚶对海洋小球藻的滤除率逐步升高,24℃时的最大滤除率为1.849 4(mL/ind.min),24℃~26℃时的滤除率逐步下降。22℃为毛蚶对微绿球藻、球等鞭金藻3011和三角褐指藻的最佳滤食温度,最大滤除率分别为1.142 4、3.804 5和4.430 4。水温8℃~24℃之间,毛蚶对巴夫藻的滤除率变化不明显,平均滤除率为2.797 2。毛蚶不仅对不同大小、形状的藻类具有选择性,而且对大小、形状相似的藻类也具有明显的选择性。水温22℃下,毛蚶对5种单胞藻的表观消化状况不同。贝类对其滤除率大、消化状况差的藻类在促进贝类净化进程中有良好的应用参考价值。  相似文献   

光照强度对九孔鲍幼虫及幼鲍生长存活的影响   总被引：7，自引：0，他引：7       下载免费PDF全文

严正凛 《水产学报》2001,25(4):336-341

在自然光周期下，分别在高温长光照和低温短光照的两个节季节，采用控制光照强度的方法进行对九孔鲍幼虫及幼鲍50d左右培育的小型试验。结果表明，光照强度对附着基上摄食微藻的幼虫及幼鲍生长和存活等有重要的影响。对幼虫及幼鲍最适宜的光照强度，在高温长光照下为700－1200lx，在低温短光照下为1300－2000lx。选用适宜的光照强度在高温长光照和低温短光照下先后进行了3次经量育苗试验，获得了与小型试验相同的结果。  相似文献   

光照对细胞融合藻Tetraselmis sp·－1混合培养的影响     

沈继红  林学政  李光友  刘发义 《中国水产科学》2002,9(3):239-242

对细胞融合技术获得的1株新型融合微藻(Tetraselmis sp.-1)进行摇瓶混合培养,并分析光照强度对该培养条件下融合微藻的影响.结果表明1) 在培养基中添加葡萄糖对融合微藻的生长有强烈的促进作用,融合微藻生长速率为0. 576 g/(L*d),是自养培养的7.38倍;2)在光照强度为3 000 lx混合培养时,葡萄糖消耗速率达最大值3 g/(L*d);3)在光照强度为2 000 lx混合培养时,藻细胞密度最大,为2.60 g/L,以葡萄糖计算的基质得率随光照强度的增加而显著下降;4)融合微藻叶绿素a含量在混合培养条件下,随光照强度的增大而增加;5) 在混合培养条件下,光照对NH4+的同化为正影响,融合微藻氮含量和CC/CN分别为5.68和6.06,均介于自养培养与异养培养之间.  相似文献   

Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia)     

Konstantin Chekanov  Elena Lobakova  Irina Selyakh  Larisa Semenova  Roman Sidorov  Alexei Solovchenko 《Marine drugs》2014,12(8):4504-4520

We report on a novel arctic strain BM1 of a carotenogenic chlorophyte from a coastal habitat with harsh environmental conditions (wide variations in solar irradiance, temperature, salinity and nutrient availability) identified as Haematococcus pluvialis Flotow. Increased (25‰) salinity exerted no adverse effect on the growth of the green BM1 cells. Under stressful conditions (high light, nitrogen and phosphorus deprivation), green vegetative cells of H. pluvialis BM1 grown in BG11 medium formed non-motile palmelloid cells and, eventually, hematocysts capable of a massive accumulation of the keto-carotenoid astaxanthin with a high nutraceutical and therapeutic potential. Routinely, astaxanthin was accumulated at the level of 4% of the cell dry weight (DW), reaching, under prolonged stress, 5.5% DW. Astaxanthin was predominantly accumulated in the form of mono- and diesters of fatty acids from C16 and C18 families. The palmelloids and hematocysts were characterized by the formation of red-colored cytoplasmic lipid droplets, increasingly large in size and number. The lipid droplets tended to merge and occupied almost the entire volume of the cell at the advanced stages of stress-induced carotenogenesis. The potential application of the new strain for the production of astaxanthin is discussed in comparison with the H. pluvialis strains currently employed in microalgal biotechnology.  相似文献   

Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum     

Mary L. Hamilton  Stephen Powers  Johnathan A. Napier  Olga Sayanova 《Marine drugs》2016,14(3)

We have created via metabolic engineering a heterotrophic strain of Phaeodactylum tricornutum that accumulates enhanced levels of the high value omega-3 long chain polyunsaturated fatty acid (LC-PUFAs) docosahexaenoic acid (DHA). This was achieved by generation of transgenic strains in which the Δ5-elongase from Ostreococcus tauri was co-expressed with a glucose transporter from the moss Physcomitrella patens. This double transformant has the capacity to grow in the dark in liquid medium supplemented with glucose and accumulate substantial levels of omega-3 LC-PUFAs. The effects of glucose concentrations on growth and LC-PUFA production of wild type and transformed strains cultivated in the light and dark were studied. The highest omega-3 LC-PUFAs accumulation was observed in cultures grown under mixotrophic conditions in the presence of 1% glucose (up to 32.2% of total fatty acids, TFA). Both DHA and EPA are detected at high levels in the neutral lipids of transgenic cells grown under phototrophic conditions, averaging 36.5% and 23.6% of TFA, respectively. This study demonstrates the potential for P. tricornutum to be developed as a viable commercial strain for both EPA and DHA production under mixo- and heterotrophic conditions.  相似文献   

基于卷积神经网络的微藻种类识别     

崔雪森  田晓清  康伟  朱浩朋  张胜茂  JOE Silke  戴阳  樊成奇 《上海海洋大学学报》2021,30(4):710-717

微藻在生态系统的结构和功能中具有极为重要的作用,而传统光学人工镜检方法对微藻种类鉴别具有较大的难度。本研究将微藻的光学图像进行了采样,并结合国内外专家对微藻鉴定的经验知识,制作了微藻图像数据集,并进行了数据增强处理。借助深度学习的原理和方法,构建了基于卷积神经网络结构的深度学习模型（AlexNet）,对模型进行了训练,并利用5折交叉验证方法确保模型的稳定性。结果表明,模型的训练精度可达到98.78±0.98%,测试精度达85.46±0.23%,达到了预期效果。利用AlexNet模型训练得到的参数,对预留的280个样本图像进行实际测试,7个藻种的平均精确度、平均召回率和平均F1 Score分别为0.832,0.844和0.833。表明深度学习方法是鉴定微藻的一种有效方法。  相似文献   

Marine carotenoids: biological functions and commercial applications     

Vílchez C  Forján E  Cuaresma M  Bédmar F  Garbayo I  Vega JM 《Marine drugs》2011,9(3):319-333

Carotenoids are the most common pigments in nature and are synthesized by all photosynthetic organisms and fungi. Carotenoids are considered key molecules for life. Light capture, photosynthesis photoprotection, excess light dissipation and quenching of singlet oxygen are among key biological functions of carotenoids relevant for life on earth. Biological properties of carotenoids allow for a wide range of commercial applications. Indeed, recent interest in the carotenoids has been mainly for their nutraceutical properties. A large number of scientific studies have confirmed the benefits of carotenoids to health and their use for this purpose is growing rapidly. In addition, carotenoids have traditionally been used in food and animal feed for their color properties. Carotenoids are also known to improve consumer perception of quality; an example is the addition of carotenoids to fish feed to impart color to farmed salmon.  相似文献