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AIM: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. METHODS: The contraction of venous ring was measured by means of perfusion in vitro. The intimal proliferation and proliferation of cultured smooth muscle cells (vascular smooth muscle cells, VSMCs) were observed by the means of computerised image analysis and MTT method, respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. RESULTS: 1 week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they were more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control). TEA (blocker of Ca2+ activated potassium channels) increased MTT A490 value of VSMCs from femoral vein in a dose-dependent manner (P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts 1-4 weeks after transplantation (P<0.05, n=5). CONCLUSION: KCa was inhibited in autogenous vein graft, which accounted for vasospasm and intimal proliferation. 相似文献
23.
根据不同退化程度草原和不同开垦年限农田土壤137Cs放射强度分析结果表明:与轻度退化草原相比,中度退化和重度退化中的137Cs放射强度分别下降了21%和52%。草原土壤开垦后,137Cs放射强度明显下降,开垦7年、15年、33年后,137Cs的放射强度分别只有轻度退化草原的37%、31%和26%。相关分析表明,伴随着土壤侵蚀的发生,土壤有机质含量、全N含量以及阳离子交换量下降。137Cs放射强度与土壤有机碳、土壤全N、交换性K和阳离子交换量呈极显著的正相关。 相似文献
24.
《Veterinary parasitology》2015,207(1-2):64-71
Syngamus trachea is a pathogenic tracheal nematode that causes syngamiasis in wild and game birds, especially when birds are managed at high densities. Despite its pathogenic nature, very little is known about its epidemiology and relationship with ambient temperature and humidity. The spatial and temporal modelling of disease was undertaken on two pheasant estates within the South West of England from April 2014 to August 2014. Significant differences between the mean numbers of eggs per gram of soil were identified between pens at both site 1 and site 2 but did not differ significantly between sites. Egg abundance was significantly associated with soil moisture content, with greater egg survival between years in pens with higher average volumetric soil moisture content. Previous years stocking density and pen age were also associated with greater egg survival between years with more eggs being recovered in pens with greater stocking densities, and pens that had been sited longer. The greatest model to explain the variation in the numbers of eggs per gram of soil per pen was a combination of soil moisture content, stocking density and pen age.Larval recovery differed significantly between sites. Larval abundance was significantly and positively associated with temperature and relative humidity at site 1. Similarly, temperature and humidity were also positively and significantly associated with larval abundance at site 2. Rainfall did not influence larval recovery at either site 1 or site 2. The model with the greatest ability to explain larval abundance at both sites, was a combination of temperature, humidity and rainfall. Infection status (positive faecal egg counts) was significantly and positively associated with larval abundance at both sites, but rainfall was only positively associated at site 1. Temperature and humidity were positively associated with infection status at site 2, but not at site 1. The present study highlights the influence of climatic variables on both egg survival and larval abundance, and could therefore be used to develop more targeted treatment strategies around periods of higher disease risk. The frequent use of release pens is a clear factor in the epidemiology of syngamiasis, and it is recommended that pens be rested and/or rotated in order to reduce infection pressure in subsequent flocks. 相似文献
25.
黄土高原人工灌草系统不同立地条件土壤种子库特征 总被引:2,自引:0,他引:2
本研究通过野外调查取样与室内试验相结合,连续两年对黄土高原人工灌草系统不同坡向、坡位进行取样,以期对该区土壤种子库的物种组成、密度特征及其物种多样性进行初步了解。研究结果表明:该人工灌草生态系统土壤种子库共有9科,15种,其组成因坡位、坡向以及年份而异;坡向、坡位均显著影响土壤种子库物种多样性指数、丰富度指数与土壤种子库密度(P<0.05),土壤种子库密度为3 218~5 492粒·m-2,总体呈现为阴坡显著高于阳坡(P<0.05),下坡位显著高于上坡位(P<0.05);坡位和坡向均对物种均匀性系数无显著影响;年份对土壤种子库无显著影响。上述研究可为黄土高原人工灌草系统的可持续利用与恢复提供参考依据。 相似文献
26.
27.
In Central Europe, various plant species including large-grain legumes and their mixtures are grown as catch crops, particularly between grains harvested early and subsequent summer crops. This article investigates the question of how soil structure in the topsoil is influenced when catch cropping with large-grain legumes (experimental factor A: without catch crop, with catch crop) under different ploughless tillage conditions during catch crop seeding (experimental factor B: deep tillage/25–30 cm, shallow tillage/8–10 cm). Five one-year trials were performed using standard machinery at various sites in Germany. Soil core samples extracted from the topsoil in the spring after catch crop cultivation served to identify air capacity, saturated hydraulic conductivity and precompression stress. The above-ground and below-ground biomass yields of the catch crops were also determined at most of the sites. In addition, the soil compaction risk for the working steps in the experiments was calculated using the REPRO model.The dry matter yield of the catch crops varied considerably between the individual trial sites and years. In particular, high levels of dry matter were able to form in the case of early seeding and a sufficient supply of precipitation. The soil structure was only rarely affected positively by catch crop cultivation, and catch crops did not contribute in the short term to loosening already compacted topsoils. In contrast, mechanical soil stresses caused by driving over the ground and additional working steps used in cultivating catch crops often led to lower air capacity in these treatments. This is consistent with the soil compaction risks calculated using the REPRO model, which were higher in the treatments with catch cropping. Catch crop cultivation also only resulted in improved mechanical stability at one location. The positive effect of deep ploughless tillage on air capacity and saturated hydraulic conductivity, however, became more clearly evident regardless of catch crop cultivation. In order for catch crop cultivation with large-grain legumes to be able to have a favourable impact on soil structure, it is therefore important that cultivating them does not result in any new soil compaction. In the conditions evaluated, deep tillage was more effective at loosening compacted topsoil than growing catch crops. 相似文献
28.
Benefits of organic farming on soil fauna have been widely observed and this has led to consider organic farming as a potential approach to reduce the environmental impact of conventional agriculture. However, there is still little evidence from field conditions about direct benefits of organic agriculture on soil ecosystem functioning. Hence, the aims of this study were to compare the effect of organic farming versus conventional farming on litter decomposition and to study how this process is affected by soil meso- and macrofauna abundances. Systems studied were: (1) organic farming with conventional tillage (ORG), (2) conventional farming with conventional tillage (CT), (3) conventional farming under no-tillage (NT), and (4) natural grassland as control system (GR). Decomposition was determined under field conditions by measuring weight loss in litterbags. Soil meso- and macrofauna contribution on decomposition was evaluated both by different mesh sizes and by assessing their abundances in the soil. Litter decomposition was always significantly higher after 9 and 12 months in ORG than in CT and NT (from 2 to 5 times in average), regardless decomposer community composition and litter type. Besides, mesofauna, macrofauna and earthworm abundances were significantly higher in ORG than in NT and CT (from 1.6 to 3.8, 1.7 to 2.3 and 16 to 25 times in average, respectively for each group). These results are especially relevant firstly because the positive effect of ORG in a key soil process has been proved under field conditions, being the first direct evidence that organic farming enhances the decomposition process. And secondly because the extensive organic system analyzed here did not include several practices which have been recognized as particularly positive for soil biota (e.g. manure use, low tillage intensity and high crop diversity). So, this research suggests that even when those practices are not applied, the non-use of agrochemicals is enough to produce positive changes in soil fauna and so in decomposition dynamics. Therefore, the adoption of organic system in an extensive way can also be suggested to farmers in order to improve ecosystem functioning and consequently to achieve better soil conditions for crop production. 相似文献
29.
By burrowing galleries and producing casts, earthworms are constantly changing the structure and properties of the soils in which they are living. These changes modify the costs and benefits for earthworms to stay in the environment they modify. In this paper, we measured experimentally how dispersal behaviour of endogeic and anecic earthworms responds to the cumulative changes they made in soil characteristics. The influence of earthworm activities on dispersal was studied in standardised mesocosms by comparing the influence of soils modified or not modified by earthworm activities on earthworm dispersal rates.The cumulative use of the soil by the earthworms strongly modified soil physical properties. The height of the soil decreased over time and the amount of aggregates smaller than 2 mm decreased in contrast to aggregates larger than 5 mm that increased. We found that: (i) earthworm activities significantly modified soil physical properties (such as bulk density, soil strength and soil aggregation) and decreased significantly the dispersal rates of the endogeic species, whatever the species that modified the soil; (ii) the decreasing in the dispersal proportion of the endogeic species suggests that the cost of engineering activities may be higher than the one of dispersal; (iii) the dispersal of the anecic species appeared to be not influenced by its own activities (intra-specific influences) or by the activities of the endogeic species (inter-specific influences). Overall these results suggest that the endogeic species is involved in a process of niche construction, which evolved jointly with its dispersal strategy. 相似文献
30.
土壤中根皮苷和根皮素对桃幼苗的影响 总被引:1,自引:0,他引:1
以一年生桃嫁接苗为试材,在盆栽条件下研究了苹果园土壤中特征酚酸类物质——根皮苷和根皮素对桃苗生物量、根系活力、根系保护性酶活性和MDA含量的影响。结果表明,根皮苷对桃幼苗的伤害显著,与对照相比,鲜质量、干质量分别降低了16.66%和21.58%,根系活力降低了33.04%,SOD、POD活性分别降低了40.80%和31.10%,MDA含量提高了116.34%。根皮素对桃苗的影响不大。高锰酸钾处理明显缓解了根皮苷对桃苗的伤害,苹果园土壤中的根皮苷含量降低了57.80%,提高了桃苗的生物量、根系活力和保护性酶活性,降低了MDA含量。综上,老龄苹果园土壤实测含量的根皮苷(0.343mg·kg~(-1))可降低桃苗的生物量,适量的高锰酸钾能缓解根皮苷的伤害作用。 相似文献