菠菜叶绿素提取方法的比较研究   总被引：4，自引：0，他引：4  

张秀君  孙钱钱  乔双  朱海  孙丹  江丕文 《作物杂志》2011,(3):57-60

以菠菜为对象,用不同有机溶剂处理,对研磨法、浸提法和超声波萃取法提取叶绿素的效果进行比较研究。结果表明,3种提取方法的最佳提取剂分别为:研磨法为丙酮与无水乙醇1∶1、直接浸提法和超声波萃取法均为丙酮与95%乙醇2∶1加少许碳酸钙;后两种方法提取的叶绿素含量比研磨法分别高53.5%和45.1%;混合液提取效果比单一试剂好,可以认为是一种协同萃取效应。综合比较确定超声波萃取法是最佳的提取叶绿素方法。  相似文献   

菠菜硝酸盐含量遗传的初步研究   总被引：3，自引：0，他引：3  

林家宝  陈火英  林观捷 《上海交通大学学报(农业科学版)》1995,(2)

本文研究了蔬菜性状特征对ＮＯ３－含量变化的关系，指出叶片光滑、色淡的类型要比叶片皱缩、色深的类型ＮＯ３－含量低。通过亲子代硝酸盐含量变异的分析以及对杂交后代群体不同株系硝酸盐含量变化的分布状况，初步认为蔬菜硝酸盐含量主要由累加基因所控制，可通过筛选低ＮＯ３－的亲本杂交，有可能获得ＮＯ３－含量低且高产的杂交一代品种，育种实践验证了上述分析的正确性。  相似文献   

Trace elements in spinach (Spinacia oleracea) cultivated in soil fortified with graded levels of iron     

N. S. Reddy  T. N. Khan  V. G. Malewar  K. B. Dudde 《Plant foods for human nutrition (Dordrecht, Netherlands)》1995,47(4):357-360

Trace elements in two varieties of spinach cultivated in soil with different levels of added iron were determined. Addition of iron to soil decreased potassium, sodium and magnesium contents in spinach markedly (p<0.05), while the contents of zinc, manganese and copper in spinach were not significantly altered (p>0.05). Differential behaviour of spinach varieties was found in the zinc, manganese and sodium contents.  相似文献   

Effect of home processing on ascorbic acid and β-carotene content of spinach (Spinacia oleracia) and amaranth (Amaranthus tricolor) leaves     

Shashi Kala Yadav  Salil Sehgal 《Plant foods for human nutrition (Dordrecht, Netherlands)》1995,47(2):125-131

The present investigation was conducted to study the concentration of ascorbic acid and -carotene in spinach and amaranth leaves as affected by various domestic processing and cooking methods which included storage of leaves in polythene bags or without packing for 24 and 48 hours in refrigerator at 5 °C; at 30 °C in polythene bags; drying (sun and oven); blanching (5, 10, 15 min); open pan and pressure cooking. Ascorbic acid content of fresh leaves was 624.1 to 629.0 mg and -carotene content was 35.3 to 53.1 mg/100 g dry weight. The percent loss of ascorbic acid ranged from 1.1 to 6.3 and 55.3 to 65.9 while lower losses (0.0 to 1.3 and 1.5 to 2.1) of -carotene were observed in leaves stored in refrigerator and at 30 °C, respectively. A markedly greater reduction in ascorbic acid and -carotene was observed in dried, blanched and cooked leaves. The study recommended the storage of leaves in refrigerator, drying in oven, blanching for shorter time and cooking in pressure cooker for better retention of these two vitamins.  相似文献   

氯化胆碱浸种对菠菜种子萌发·幼苗某些生理特性的影响     

王素蓉  周晓丽  周峰  华春 《安徽农业科学》2011,39(3):1295-1297

[目的]用不同浓度的氯化胆碱（CC）溶液浸种后,研究CC对菠菜种子萌发及幼苗某些生理特性的影响。[方法]测定了种子萌发率、发芽指数、蛋白质、叶绿素和可溶性糖含量、抗氧化酶活性、丙二醛含量和超氧阴离子的产生速率。[结果]CC溶液浸种后能提高种子萌发率和发芽指数,提高幼苗叶片中可溶性糖、可溶性蛋白质和叶绿素含量,超氧化物歧化酶、过氧化氢酶和过氧化物酶的活性均有所提高,而超氧阴离子的产生速率和丙二醛含量明显低于未经CC处理的。[结论]各浓度的CC溶液浸种以25 mg/L浸种效果最好。  相似文献   

Performance of two <Emphasis Type="Italic">Trichogramma</Emphasis><Emphasis Type="Italic">brassicae</Emphasis> strains under greenhouse and field conditions for biocontrol of the silver Y moth in spinach cultures     

Thomas Klug  Rainer Meyhöfer 《Journal of pest science》2009,82(1):73-79

The suitability of Trichogramma brassicae Bezd. (Hymenoptera: Trichogrammatidae) to control the silver Y moth, Autographa gamma (L.) (Lepidoptera: Noctuidae) in spinach was investigated under greenhouse and field conditions. Two strains of T. brassicae were selected to study host searching efficiency and dispersal ability of the wasps on spinach. The experiments were conducted with defined release densities. The results show that T. brassicae strain I failed to locate host eggs in all experiments. In contrast, T. brassicae strain II females were able to locate 37% of the exposed egg clusters in the greenhouse within 48 h. Individuals colonised rapidly at least an area of 0.25 m². At distances up to 26 cm to the release point of the T. brassicae females, parasitization rate was 100%. Moreover, under field conditions silver Y moth eggs were exposed two times for 48 h on 12 experimental plots of 100 m² in two spinach fields. During the first exposure interval, i.e. 4 days after mass release of T. brassicae strain II, overall parasitization rates reached 20% on both fields. Furthermore parasitization rates still reached 16 and 19% during the second exposure interval, i.e. 9 days after natural enemy release, indicating a constant Trichogramma activity over time. Parasitization rates were highest in the close vicinity of the release point, i.e. 86%, whereas 6.5 m away the mean parasitization rate decreased to 3%. In conclusion results indicate a high potential of T. brassicae strain II as antagonist of A. gamma. Nevertheless the dispersal ability of T. brassicae strain II was limited, but adjustment of release densities and techniques might compensate this disadvantage.  相似文献   

水生蕹菜对沼液净化效果的研究          下载免费PDF全文

叶美锋  吴晓梅  吴飞龙  林代炎 《农学学报》2017,7(3):73-76

为研究水生经济植物对沼液净化处理的效果,本研究开展水生蕹菜对不同浓度沼液的净化试验,通过测试水生蕹菜对沼液中COD、TN、TP的去除效率及其生化指标,分析水生蕹菜的食品安全性与经济效益。试验结果表明：水生蕹菜可以在高浓度沼液中栽培成活,并对沼液具有较强的净化效果;稀释40倍沼液COD去除率最高,稀释30倍的沼液水培水生蕹菜经济效益最好,重金属指标符合绿色食品水生蔬菜(NY/T 1405—2015)标准要求。通过沼液种植水生蕹菜既能充分利用沼液中的营养物质,又能减少养猪场污染物排放,而且其食品安全性又有保障,具有良好的环境与经济效益。  相似文献   

Ca（2＋）和茉莉酸对菠菜CBF表达的影响     

于涌鲲  赵福宽  万善霞  郝玉兰  孙清鹏 《农业科学与技术》2011,(11):1574-1575,1661

[目的]为探究钙离子、茉莉酸（Jasmonic acid,JA）对菠菜CBF转录因子表达的影响。[方法]以菠菜（Spinacia oleraceaL.）为材料,对其进行低温（4℃）或JA处理或用钙离子载体A23187预处理菠菜幼苗,利用Northern印迹技术检测各处理时CBF的表达情况。[结果]低温和JA均可诱导菠菜中CBF基因的表达。[结论]推测低温可能首先导致细胞中JA含量升高,JA通过一定的机制诱导细胞质基质中游离钙离子浓度（[Ca2＋]cyt）升高,然后Ca2＋可能通过CaM（Calmodulin）或CaM相关蛋白传递低温信号,从而诱导CBF的表达。  相似文献   

制革下脚料酶解物对菠菜生长及品质的影响     

李涛  姚建刚  曹守军  夏秀波  张丽莉  王虹云  周杨  尹国香  李素梅  王吉腾 《安徽农业科学》2017,45(35):53-54,59

[目的]研究制革下脚料酶解物对菠菜生长及品质的影响。[方法]以菠菜品种"先锋388"为试材,施用不同量的制革下脚料酶解物,评价其对菠菜形态学性状、产量和品质的影响。[结果]酶解物最佳施用量为6.00 kg/m~2,施用制革下脚酶解物处理的菠菜形态学性状和产量与施用同类型有机肥相比较无显著性差异,其可溶性总糖和维生素C含量显著提高。[结论]施用制革下脚料酶解物,在保证菠菜产量的同时可以显著提高菠菜的品质。  相似文献   

菠菜内生细菌SEB的鉴定及拮抗作用     

吴赟生  张森泉  张荣  李平东 《安徽农业科学》2008,36(20)

[目的]从菠菜上筛选内生细菌,防治菠菜主要病害。[方法]用稀释分离法从菠菜植株内分离内生细菌,培养基平板对峙法测定内生细菌的拮抗作用,并依据形态特征和生理生化特性,使用B iolog细菌鉴定系统鉴定。[结果]从菠菜植株根分离到具有生防活性的细菌SEB,对菠菜枯萎病菌等5种病原菌有较强的拮抗作用,鉴定为芽孢杆菌属。[结论]SEB菌株对多种病原真菌有拮抗作用,对菠菜枯萎病菌抑制率最高,具有开发成微生物制剂,防治园艺作物和农作物病害枯萎病的价值。  相似文献