Reactivity of workshop monoclonal antibodies on paraformaldehyde-fixed porcine blood mononuclear cells     

H. -Joachim Schuberth  H. -Udo Rabe  Wolfgang Leibold 《Veterinary immunology and immunopathology》1998,60(3-4):409-417

One hundred sixty-four monoclonal antibodies (mAbs) of the second international swine CD workshop were tested for their reactivity with porcine blood mononuclear cells before and after fixing the cells with varying concentrations of paraformaldehyde (PFA) (1, 5 and 10 g l⁻¹). A total of 38 (out of 134) positive reacting mAbs were significantly affected in their binding behavior on fixed cells. Modulation was seen as reduction in binding (staining intensity and/or % positive cells, n=18) or in elevated values (n=20). Modified mAb binding occurred after fixing cells with 5 to 10 g l⁻¹ PFA.  相似文献   

莱芜猪肌肉组织学特性与肉质关系的研究   总被引：56，自引：4，他引：52  

曾勇庆  孙玉民 《畜牧兽医学报》1998,29(6):486-492

本研究采用二因子多水平有重复的试验方法,对１４头育肥莱芜猪不同屠宰体重及不同解剖部位肌肉的组织结构和肌纤维的组织学特性及肉质性状进行研究。结果表明：随屠宰体重的提高,肌肉纤维直径（ＤＭＦ）,密度（ＭＦＤ）和宰后僵直肌工降度（ＳＬ）没有发生显著变化（Ｐ〉０．０５）,组织学显示的肌肉３种构成分在不同体重组间差异也不显著,但除ｐＨ外,其他各测定性状在不同部位肌肉都存在极显著的差异（Ｐ〈０．０１）,研究下  相似文献   

猪2型链球菌河北株的分离及PCR鉴定     

范京惠  左玉柱 《勤云标准版测试》2007,(6)

对河北省某规模化猪场病死猪进行临床诊断和实验室诊断,通过采取病料直接涂片染色镜检,病原分离培养,初步怀疑为链球菌。对纯化的分离细菌进行动物试验、生化试验,确定该病原菌为链球菌。分离菌经过液体增菌后,提取细菌基因组DNA,用荚膜多糖基因的特异性引物分别扩增出与设计大小相符条带,将PCR产物送至上海生物工程有限公司进行序列测定,并进行序列分析,确定为该菌株为猪链球菌2型。  相似文献   

β-肾上腺素能激动剂对肥育猪生产性能的影响     

陈国胜  罗志忠  张建远 《信阳农业高等专科学校学报》1998,(3)

本试验采用饲养试验及活体测定的方法，对20头杜长大三元杂交肥育猪（体重60千克左右）进行试验，试验组日粮中添加SPPM的肾上腺素能激动剂（Clenbuterol）。结果表明，在相同的营养水平和饲养管理条件下，试验组猪比对照组背膘厚降低14．8％，眼肌面积增加9．7％．日增重提高9．4％。  相似文献   

猪瘟乳前免疫及其抗体检测     

何存利  陈祝三  谢琴  史建斌  马学校  雍建华  赵保全  李宁新  王诚 《中国预防兽医学报》1998,(3)

本试验对新生仔猪哺乳前初次进行猪瘟疫苗免疫，至７０日龄时进行第二次免疫，免疫持续至７０日龄和１５０日龄时两次应用Ｄｏｔ－ＥＬＩＳＡ方法进行检测，结果表明，阳性率分别为９２．３％和９１．２５％，说明此免疫方法是一较好的预防猪瘟的免疫程序。  相似文献   

畜舍内混合气体定量检测系统的研究     

葛广军  杨师斌  王俊 《安徽农业科学》2007,35(12):3744-3745

将气体传感器阵列与神经网络模式识别技术相结合,构建了一个多组分混合气体定量检测系统,进行了初步的试验,并对试验方法进行了改进.结果表明,用该方法识别准确率接近93%,为畜舍内空气质量的在线检测打下了良好的基础.  相似文献   

Bacteriophages as antimicrobial agents against major pathogens in swine: a review     

Jiancheng Zhang  Zhen Li  Zhenhui Cao  Lili Wang  Xiaoyu Li  Shuying Li  Yongping Xu 《畜牧与生物技术杂志(英文版)》2015,6(1)

In recent years, the development of antibiotic resistant bacteria has become a global concern which has prompted research into the development of alternative disease control strategies for the swine industry. Bacteriophages (viruses that infect bacteria) offer the prospect of a sustainable alternative approach against bacterial pathogens with the flexibility of being applied therapeutically or for biological control purposes. This paper reviews the use of phages as an antimicrobial strategy for controlling critical pathogens including Salmonella and Escherichia coli with an emphasis on the application of phages for improving performance and nutrient digestibility in swine operations as well as in controlling zoonotic human diseases by reducing the bacterial load spread from pork products to humans through the meat.  相似文献   

Detection of Novel Reassortant Influenza A (H3N2) and H1N1 2009 Pandemic Viruses in Swine in Hanoi,Vietnam          下载免费PDF全文

T. D. Dao  N. T. Pham  B. J. Cowling  M. Peyre  M. Peiris 《Zoonoses and public health》2015,62(6):429-434

From May to September 2013, monthly samples were collected from swine in a Vietnamese slaughterhouse for influenza virus isolation and serological testing. A(H1N1)pdm09 viruses and a novel H3N2 originating from reassortment between A(H1N1)pdm09 and novel viruses of the North American triple reassortant lineage were isolated. Serological results showed low seroprevalence for the novel H3N2 virus and higher seroprevalence for A(H1N1)pdm09 viruses. In addition, serology suggested that other swine influenza viruses are also circulating in Vietnamese swine.  相似文献   

Up-regulated expression of PD-1 and its ligands during acute Classical Swine Fever virus infection in swine     

Feng Yue  Yan-Ping Zhu  Yan-Fang Zhang  Guo-Peng Sun  Yuan Yang  Dong-Guang Guo  Ai-Guo Wang  Bo-Wen Li  Mei Yin  An-Chun Cheng  Ming-Shu Wang  Xuan-Nian Wang 《Research in veterinary science》2014

In order to investigate the relationship between the PD-1 pathway and impairment of immune responses with the CSFV infection, the mRNA expression of PD-1 and its ligands were evaluated by quantitative polymerase chain reaction (qPCR) during artificial CSFV infection. Simultaneously, expression of IL-2 and IL-10 mRNA were detected. The T cell proliferation and CSFV load in plasma were also measured. Results showed that the expression of PD-1 and its ligands mRNA were significantly increased (p < 0.01) in PBMC from 3 to 7 days post infection (dpi). Meanwhile the level of IL-10 was up-regulated (p < 0.01). The IL-2 mRNA was not obviously changed but it is significantly increased from 14 dpi. The T cell proliferation was notably decreased at 7 dpi. The CSFV load was also increased in plasma. Overall, our results suggest that the expression of PD-1 and its ligands were up-regulated and probably correlated with immune inhibition during acute CSFV infection.  相似文献   

我国市场上猪支原体肺炎疫苗现状及应用效果   总被引：1，自引：0，他引：1  

车艳杰  王贵华 《中国兽药杂志》2014,48(12):61-65

目前,市场上猪支原体肺炎疫苗主要为常规的全菌体疫苗,根据我国猪支原体肺炎产品注册情况,本文主要从猪支原体肺炎疫苗的制备技术、免疫程序及免疫效果三个方面对国内报批、销售的猪支原体肺炎疫苗进行比较研究,并简要分析各疫苗产品的特点。  相似文献