PCR-based detection of sterol demethylation inhibitor-resistant strains of Penicillium digitatum     

Hamamoto H  Hasegawa K  Nakaune R  Lee YJ  Akutsu K  Hibi T 《Pest management science》2001,57(9):839-843

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原卟啉原氧化酶抑制剂类除草剂进展概况   总被引：6，自引：0，他引：6  

张国生 《农药科学与管理》2001,22(6):21-25

本文对近期开发的原卟啉氧化酶抑制剂按用途进行了简要的介绍。文中涉及原卟啉氧化酶抑制剂的化学结构、生物活性、登记与专利和开发公司等。  相似文献   

细菌耐药颉颃剂的研究现状及应用     

娄飞  李荣誉  张龙现 《中国畜牧兽医》2007,34(9):74-77

作者介绍了具有颉颃细菌耐药性作用的物质的研究应用进展情况，包括灭活酶抑制剂、药物渗透促进剂、外输泵抑制剂、细菌生物被膜抑制剂、抗菌药物增强剂、耐药质粒消除剂等。  相似文献   

Caspase 3 inhibitor Ac-DEVD-CHO attenuates 10-hydroxycamptothecin-induced activation of NF-κB in Bcap37 cells     

YE Meng  LIN Lei  PAN Hong-ming  FANG Yong  WU Jin-min 《园艺学报》2007,23(8):1508-1511

AIM:To investigate the role of caspase 3 inhibitor Ac-DEVD-CHO in caspase 3 signaling pathway and NF-κB activation induced by 10-hydroxycamptothecin (HCPT) in human breast carcinoma cells. METHODS:The cell growth inhibition was measured by MTT assay. Agarose gel electrophoresis was performed for detecting cell apoptosis. Western blotting was used for determining protein expression. DIG-EMSA was conducted to measure the DNA-binding activation of NF-κB. RESULTS:Caspase 3 inhibitor Ac-DEVD-CHO attenuated HCPT-induced apoptosis in human breast carcinoma. Ac-DEVD-CHO also suppressed the degradation of caspase 3 and IκBα,and arrested the activation of NF-κB. CONCLUSION:Caspase 3 inhibitor Ac-DEVD-CHO regulates the activation of caspase 3 and NF-κB,and attenuates apoptosis in Bcap37 cell line induced by HCPT.  相似文献   

Effects of estrogen on expressions of MMP-2,MMP-9 and tissue inhibitors in prostate stromal cells     

XIAO Xiang-qian  FU Rong  ZHANG Li-guo  LIU Shu-ye  WU Quan  YAN Chun-he  SHI Jian-dang  ZHANG Ju 《园艺学报》2007,23(11):2248-2252

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抑制鹿源多药耐药结核菌中药的筛选   总被引：1，自引：0，他引：1  

姜园园  李温明  刘畅  曾范利  时坤  李健明  刁乃超  李仲乐  刘东旭  杜锐 《动物医学进展》2016,(4):54-57

为了筛选对鹿源多药耐药结核菌有效的中药耐药抑制剂,通过MABA法检测24种中药的水和乙醇提取物对多药耐药结核菌及标准菌H37Rv的体外抗菌效果,将筛选出的抗菌效果好的黄连醇提物和黄连水提物采用紫外分光光度法进行活性成分含量测定,同时以MABA法与标准品比较体外抗结核菌活性。结果显示,黄连、独活和侧柏叶有明显的抗结核活性,其中黄连水粗提物和乙醇粗提物效果最佳,测得它们活性成分即总生物碱含量分别为22.74%和35.23%,并显示二者与小檗碱的体外抗结核作用相同。  相似文献   

核桃楸枝条,插穗中生根抑制物质的含量   总被引：9，自引：0，他引：9  

徐程扬  张忠辉 《吉林林学院学报》1998,14(4):212-215

通过红心萝卜种子发芽试验表明核桃楸母树体内存在着生根抑制物质．枝皮内的生根抑制物质含量随母树年龄的增加而提高；生根抑制物质含量在高生长旺盛期枝皮内最低，在休眠期含量最高；平茬具有较强的生理复幼作用，根萌枝条皮内的生根抑制物质含量较低；插穗在扦插过程中皮内生根抑制物质含量逐渐降低．  相似文献   

硝化抑制剂缓释尿素试验效果初探   总被引：3，自引：0，他引：3  

李冬梅  王绍新  刘芳  柳维刚  初艳萍 《北方水稻》2008,38(3):96-97

硝化抑制剂缓释尿素具有提高氮素利用率、增产、增收、节肥等功效。为验证其在水稻上的应用效果,特进行此试验。结果表明:底肥一次性施用硝化抑制剂缓释尿素或第一代缓释尿素对水稻具有不同程度的增产效果,分别增产6.7%、3.9%,净增收76.35元/667 m2、52.19元/667 m2,节省肥料、节省功时费,在生产上有一定推广价值。  相似文献   

Increase of in vitro regeneration in Malus domestica by the application of phosphatase inhibitors   总被引：1，自引：0，他引：1  

C. Graph  G. Wricke 《Plant Breeding》1998,117(6):563-566

The effect of the phosphatase inhibitors sodium fluoride and sodium orthovanadaate on adventitious shoot regeneration from primary leaf explants was tested in eight genotypes of apple Malus domestica Borkh. Ten different concentrations between 0.5 and 100μM of each substance were added to a modified Murashige and Skoog regeneration medium containing 0.9 μM TDZ and 0.5 μM IBA. In general, all the con-centrations tested were able to promote shoot formation, although distinct maxima could be observed depending upon the genotype and the inhibitor. Shoot yield was influenced mostly by the genotype and by the concentration of the inhibitor, as well as the interaction between these two factors. In six of the eight genotypes the total number of regenerated shoots increased significantly compared with the control. This was achieved by an increase of the number of regenerating explants as well as the number of shoots per regenerating explant Comparing these results with those of former publications, application of the two phosphatase inhibitors resulted in an improved regeneration response in the corresponding genotypes. This method may also contribute to the understanding of in vitro regeneration in plants.  相似文献   

Alpha-amylase/subtilisin Inhibitor Levels in Australian Barleys     

S.J Jarrett  RJ Marschke  M.H Symons  C.E Gibson  R.J Henry  G.P Fox 《Journal of Cereal Science》1997,25(3):261-266

An enzyme-linked immunosorbent assay (ELISA) was used to determine the bifunctional alpha-amylase/subtilisin inhibitor (BASI) content of barley grain from 11 cultivars grown in six diverse locations in Australia. The inhibitor ranged from 119 to 254 μg/g in 57 barley samples. Genotype had a significant (P<0·05) effect on BASI content but there was no effect due to environment. Total protein varied independently of BASI and was influenced by environment and genotype. BASI content was higher (P<0·05) in malting barley than in feed barley and was correlated positively (r=0·29;P<0·05) with alpha-amylase activity in corresponding malts. The ELISA used monoclonal and polyclonal antibodies raised against purified BASI. In immunoblot analysis the monoclonal antibody showed high specificity for the inhibitor in barley and also detected the inhibitor in wheat. Low levels of inhibitor (mean 3·2 μg/g) were found in 12 Australian wheat cultivars using the ELISA developed for barley. The assay had a linear working range of 5–50 ng/mL with a detection limit of 2 ng/mL. Reproducibility between assays was good (CV=4·9%) but mean recoveries were high, ranging from 116–129% when purified inhibitor was added to barley extracts. The ELISA may have useful applications in brewing research and barley breeding programmes.  相似文献