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随着规模化养殖业发展,兽用抗生素大量使用,不合理甚至违法添加使用导致的残留现象已成为威胁动物源食品安全的重要因素,迫切需要建立灵敏可靠的残留分析方法。从复杂的生物样品中提取纯化微量的残留药物是制约兽药残留检测方法建立的关键。分子印迹技术具有特异性强、灵敏度高、操作简便等优点,在兽药残留检测研究中得到越来越多重视。文章主要综述了分子印迹聚合物的最新制备方法及在兽用抗生素残留分析中的最新研究进展。 相似文献
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G.W. Smith J.L. Davis S.M. Smith M.P. Gerard N.B. Campbell D.M. Foster 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2010,24(4):949-955
Background: Despite frequent clinical use, information about the pharmacokinetics and efficacy of pantoprazole in camelids is not available. Objectives: To examine the pharmacokinetics of both IV and SC pantoprazole and to determine whether pantoprazole administration would increase 3rd compartment pH in alpacas. Animals: Six healthy adult alpacas. Methods: Alpacas were fitted with a 3rd compartment cannula for measuring gastric pH. After recovery, alpacas received 1 mg/kg pantoprazole IV, q24h for 3 days or 2 mg/kg SC q24h for 3 days. Alpacas received both IV and SC pantoprazole, with a minimum of 3 weeks between treatments. Third compartment pH was recorded and plasma samples were taken for pharmacokinetic analysis. Results: Pantoprazole induced a slow but sustained increase in 3rd compartment pH when given by both the IV and SC routes. Third compartment pH was significantly increased as compared with baseline values (1.81 ± 0.7; mean ± SD) at 24 (2.47 ± 0.8), 48 (3.53 ± 1.0) and 72 hours (4.03 ± 1.3) after daily IV administration of pantoprazole. Third compartment pH increased from 1.73 ± 0.6 at baseline to 3.05 ± 1.1, 4.02 ± 1.4, and 3.61 ± 1.6 at 24, 48, and 72 hours after SC administration, respectively. Pharmacokinetic analysis demonstrated that pantoprazole had a short elimination half‐life (0.47 + 0.06 h) and a high clearance rate (12.2 ± 2.9 mL/kg/min) after both IV and SC administration. Conclusions and Clinical Relevance: Based on the results of this study, pantoprazole represents a safe and effective drug for increasing 3rd compartment pH in camelids. Either IV or SC administration is likely to be an effective treatment for gastric ulcers. 相似文献
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The general role of veterinary epidemiology and economics to national animal-disease control throughout the world is considered for the four main groupings of animal diseases: zoonotic, food-borne, endemic and epidemic diseases. This is done by considering how veterinary epidemiology and economics has contributed to priority setting (which diseases come first?), decision-making (for a given disease, which strategy is best?), and disease control implementation (how can optimal delivery and adoption of selected interventions best be achieved?). Within each of these categories, progress made and future opportunities are discussed. In addition, a review is made of how veterinary epidemiology and economics has been institutionalised. We conclude that veterinary epidemiology and economics holds a unique role in the development of national policies and strategies for improved animal health world-wide. However, we consider that we must capitalise more on the unique comparative advantage of the partnership between veterinarians and agricultural economists. We believe that much remains to be done to improve the "institutionalisation" of veterinary epidemiology and economics, and the adoption and impact of the products of our unique partnership, particularly in countries of the developing world. 相似文献
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Heath TJ 《Australian veterinary journal》1999,77(9):584-587
OBJECTIVE: To estimate the costs to the student of veterinary education. METHOD: A questionnaire was completed by 180 fourth-year veterinary students from the four veterinary schools in Australia. Frequencies and median costs were estimated using the SAS System for Windows 95. RESULTS: The median direct living costs were about $5000 per academic year, but were higher in Sydney and for those with additional expenses including children, pets and cars. Other costs associated with the course including text books, equipment and travel to practical work were of the order of $1000, and income foregone while doing practical work placed additional burdens on some. More than half the students from Sydney and Murdoch needed to work to provide basic necessities. Most of this work involved interaction with animals or people. The percentage of Queensland and Melbourne students needing to work was lower, 47 and 24 respectively, and fewer of those worked directly with animals or people. The students expected to repay a median of $14,000 after they graduate, with 70% of this being due to the Australian government as part of the Higher Education Contribution Scheme. CONCLUSIONS: Although parents contributed an average of between one-third and one half of the costs of obtaining a veterinary degree, the extent of parental contributions was very uneven and almost one half of all students needed to work to provide basic necessities. The average total debt on graduation was about half the annual starting salary, but those starting the course from 1997 are subject to much higher charges, and their average debt is likely to exceed one year's salary. 相似文献
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