华木莲植物群落的生态学研究   总被引：8，自引：3，他引：5  

俞志雄  郑庆 《江西农业大学学报》1999,21(2):213-217

采用线路踏查和样方调查的方法,对华木莲ＳｉｎｏｍａｎｇｌｉｅｔｉａｇｌａｕｃａＺ．Ｘ．ＹｕｅｔＱ．Ｙ．Ｚｈｅｎｇ植物群落中乔木树种的重要值,物种多样性、优势度、群落均匀度以及物种丰富度等指标进行了计测,并初步分析了群落类型、结构与特种多样性指数的关系以及该植物的生境和花木莲种群特征,最后指出华木莲为中性偏阳性树,在林内天然更新困难,在现状植被中,该种群为衰退种群。  相似文献   

Inheritance of resistance to potato virus Y in garden pepper (Capsicum annuum L.)     

O. P. Sharma  P. P. Sharma  S. C. Chowfla 《Euphytica》1989,42(1-2):31-33

Summary Bell pepper suffers considerable losses from a strain of potato virus Y (PVY^o-sbp). Crosses were attempted between two resistant lines Perennial and S41-1 and two highly susceptible bell pepper commercial cultivars California Wonder and Yolo Wonder. Studies of F₁'s, F₂'s, back crosses and F₃'s indicated that Perennial and S41-1 carry a recessive gene imparting resistance to potato virus Y.  相似文献   

福建单竹属一新种     

易同培  史军义 《四川林业科技》2005,26(5):62-63

本文报道了产于中国福建南部的竹亚科一新种.它是单竹属的有节竹.  相似文献   

山核桃水溶性成分中神经营养活性因子的分离与鉴定     

吴建峰  高飞  谢倩  黄坚钦  钱永常 《中国农学通报》2018,34(15):159-164

本研究旨在揭示山核桃水溶性成分的神经营养活性及活性因子。利用脱脂山核桃水溶性提取物处理SH-SY5Y细胞,用倒置相差显微镜观察细胞突触生长,用qPCR检测相关基因表达,使用HPLC 及LC-MS对山核桃水溶性成分神经营养活性因子进行分离鉴定。结果显示,山核桃的水溶性成分能够显著促进SH-SY5Y细胞的突触生长,并可以显著上调SH-SY5Y细胞NF160 基因的表达。HPLC及LCMS检测结果发现其水溶性成分主要组成为胞苷、尿苷、鸟苷、腺嘌呤、腺苷等。由此可知,山核桃水溶性成分通过上调NF160基因表达具有神经营养活性。  相似文献   

A highly sensitive,non‐invasive qPCR‐based strategy for direct quantification of Yersinia ruckeri in fish faeces          下载免费PDF全文

Bikramjit Ghosh  Philip B. B. Crosbie  Barbara F. Nowak  Andrew R. Bridle 《Journal of fish diseases》2018,41(9):1421-1428

Finfish with asymptomatic Yersinia ruckeri infections pose a major risk as they can transmit the pathogen and cause clinical outbreaks in stock populations. Current tools have insufficient quantitative ability for accurately detecting the trace levels of Y. ruckeri typically associated with asymptomatic infection, necessitate invasive or lethal sampling, or require long processing times. This study presents a highly sensitive qPCR‐based method, targeting part of the Y. ruckeri 16S rRNA sequence, that is capable of detecting extremely low levels of Y. ruckeri in noninvasively collected faecal samples. Quantitative precision and accuracy of faecal sample analysis was consistent, despite the complexity of the faecal matrix. The assay demonstrated linearity over a six log‐wide dynamic range. Its limit of detection (LOD) and limit of quantification (LOQ) were 4 and 10 copies of the target sequence, respectively. Sensitivity of the assay was comparable to other qPCR‐based methods without requiring invasive or lethal sampling. Applicability as a screening strategy was tested using passively collected faecal samples. Asymptomatic Y. ruckeri infection was detected in all samples, although none of the fish exhibited overt infection. This method will be beneficial for finfish disease management if developed further as a noninvasive, screening tool against asymptomatic Y. ruckeri infection.  相似文献   

抗马铃薯Y病毒病的VIGS生防剂发酵条件及施用方法优化     

刘天波  蔡海林  曾维爱  魏润洁  刘嘉裕  李帆  周志成  唐前君 《中国农业科技导报》2018,20(11):29-35

病毒诱导的基因沉默（virus-induced gene silence,VIGS）是植物病毒病防治方法之一,能够有效防治马铃薯Y病毒病。以VIGS表达载体发酵液对烟草马铃薯Y病毒病的防治效果为检测指标,筛选最佳发酵液发酵所需的培养基、发酵条件和施用方法。结果表明：VIGS表达载体发酵液最优培养基是YEP培养基;最优发酵条件为装瓶量60 mL/200 mL、初始pH 7.0、培养温度30℃、振荡培养24 h;施用方法以 “原液稀释100倍”、“剪叶后喷施+移栽时灌根”和“含PVY CP和HC-Pro基因片段的VIGS载体发酵液混合使用”的施用效果最佳。上述结果为抗马铃薯Y病毒病的VIGS生防剂发酵提供了理论依据,也为其田间应用打下基础。  相似文献   

Y58S系列组合产量、品质及抗性的综合表现     

扶定  王青林  赵万兵  霍二伟  马汉云  沈光辉  全瑞兰 《中国农学通报》2015,31(3):49-53

为选育不育起点温度低、配合力强、米质优的实用型两系不育系,以近年来新育成的22个Y58S组合为对象,对其产量、品质及病虫抗性进行研究。结果表明：平均产量为8978.7 kg/hm²,所有品质指标均达到《优质稻谷》3级标准的组合占40.9%,感稻瘟病和褐飞虱。因此,Y58S系列组合产量水平较高,稻米品质较优,主要病虫抗性相对较差。  相似文献   

Structure-Activity Relationship Study of the Neuritogenic Potential of the Glycan of Starfish Ganglioside LLG-3     

Megumi Yamagishi  Ritsuko Hosoda-Yabe  Hideki Tamai  Miku Konishi  Akihiro Imamura  Hideharu Ishida  Tomio Yabe  Hiromune Ando  Makoto Kiso 《Marine drugs》2015,13(12):7250-7274

LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling.  相似文献   

利用SSR标记对家蚕黄血基因的定位及连锁分析   总被引：3，自引：2，他引：1  

赵云坡  徐安英  李木旺  李明辉  郭秋红  孙平江  钱荷英  张月华  郭锡杰 《蚕业科学》2006,32(4):464-468

位于家蚕第2染色体的黄血基因(yellow blood,Y)控制类胡萝卜素由中肠进入血淋巴,是形成黄色茧系的基础。利用家蚕雌不发生交换的特点,采用黄茧品系KY和白茧品系C108组配正反交BC_1群体(C108×KY)×C108和C108×(C108×KY),分别记作BC_1F和BC_1M,根据已经构建的家蚕SSR分子连锁图谱,对Y基因进行了定位及连锁分析。筛选出6个与Y基因连锁的SSR标记,这些标记在BC_1F群中的所有黄血个体均表现出与(C108×KY)F_1相同的杂合型带型;而所有白血个体带型与亲本C108一致,为纯合型。利用另一个群体BC_1M构建了关于Y基因的遗传连锁图,遗传距离为18．9cM,Y基因位于15．6cM。  相似文献   

广适性两系杂交中籼新组合Y两优689的选育与应用   总被引：3，自引：0，他引：3  

荀飞琼  马国华  周海平 《杂交水稻》2011,26(3)

Y两优689是温州市农科院用恢复系R689与两系不育系Y58S配组育成的两系杂交中籼新组合,在浙江省单季籼稻区试中表现适应性广、分蘖力强、结实率高、高产稳产、米质较好、米饭口感佳等特点,适宜浙江省及中国南方稻区作单季稻栽培,2011年2月通过浙江省品种审定委员会审定.  相似文献