家畜性别控制技术的研究进展   总被引：3，自引：0，他引：3  

李平  霍明东  周景明 《中国畜牧兽医》2008,35(5):145-148

在畜牧生产中,人为控制出生家畜的性别可以降低生产成本,提高经济效益。随着科技的进步和研究的不断深入,家畜性别控制技术也在不断的完善。作者综述了X、Y精子的分离和胚胎性别鉴定这两项家畜性控技术的发展历程和研究现状,并对其今后的研究方向及应用前景进行了展望。  相似文献   

Quantity production of anther-derived haploids from a multiple disease resistant tobacco hybrid. I. Frequency of plants with resistance or susceptibility to tobacco mosaic virus (TMV), potato virus Y (PVY), and root knot (RK)     

L. G. Burk  J. F. Chaplin  G. V. Gooding  N. T. Powell 《Euphytica》1979,28(2):201-208

Summary Methods are described for producing large numbers of haploid plantlets from anthers of a flue-cured tobacco hybrid with monogenic resistance to tobacco mosaic virus, (TMV), potato virus Y (PVY) and root knot (RK), respectively. Additional details are given on colchicine treatment for converting haploids to doubled haploids (DH's) and on the frequency of spontaneous DH's among untreated plantlets. Disparate genetic ratios of TMV-resistant to TMV-susceptible plants were obtained among colchicine-treated haploid plantlets, induced DH's and untreated haploids when compared with F₂ and BC₁ progenies. Haploids (gametes) with the gene for TMV resistance occurred more frequently than expected and plantlets with the gene for RK resistance occurred less frequenctly than expected. Transmission of the gene for PVY resistance differed only slightly from Mendelian expectations. These unexpected ratios, in addition to the frequent occurrence of plastid chimeras among anther-derived plantlets, strengthened our conviction that haploidy is somehow associated with mutation.Joint contribution from the Departments of Genetics, Crop Science and Plant Pathology, North Carolina State University, and the Tobacco Research Laboratory, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Oxford, North Carolina. Paper No. 5576 of the Journal Series of the North Carolina Agricultural Experiment Station, Raleigh, North Carolina.  相似文献   

黄鳝(Monopterus albus)H-Y抗原的研究   总被引：6，自引：0，他引：6  

邹记兴 《水产科技情报》1999,26(4):177-181

利用自制的抗Ｈ－Ｙ血清,对黄鳝进行脾淋巴细胞毒性试验,检测各发育时期的性腺,脑,脾及肌肉组织,结果如下：（１）雄性有Ｈ－Ｙ抗原存在,雌性无;（２）性逆转过程中,Ｈ－Ｙ抗原从雌性到雄性是从无到有逐渐递增的;（３）黄鳝经１７α－甲基睾酮处理后,雌性,间性,雄性三个发育阶段Ｈ－Ｙ抗原的存在状况并未发现显著改变。  相似文献   

华木莲植物群落的生态学研究   总被引：8，自引：3，他引：5  

俞志雄  郑庆 《江西农业大学学报》1999,21(2):213-217

采用线路踏查和样方调查的方法,对华木莲ＳｉｎｏｍａｎｇｌｉｅｔｉａｇｌａｕｃａＺ．Ｘ．ＹｕｅｔＱ．Ｙ．Ｚｈｅｎｇ植物群落中乔木树种的重要值,物种多样性、优势度、群落均匀度以及物种丰富度等指标进行了计测,并初步分析了群落类型、结构与特种多样性指数的关系以及该植物的生境和花木莲种群特征,最后指出华木莲为中性偏阳性树,在林内天然更新困难,在现状植被中,该种群为衰退种群。  相似文献   

Inheritance of resistance to potato virus Y in garden pepper (Capsicum annuum L.)     

O. P. Sharma  P. P. Sharma  S. C. Chowfla 《Euphytica》1989,42(1-2):31-33

Summary Bell pepper suffers considerable losses from a strain of potato virus Y (PVY^o-sbp). Crosses were attempted between two resistant lines Perennial and S41-1 and two highly susceptible bell pepper commercial cultivars California Wonder and Yolo Wonder. Studies of F₁'s, F₂'s, back crosses and F₃'s indicated that Perennial and S41-1 carry a recessive gene imparting resistance to potato virus Y.  相似文献   

福建单竹属一新种     

易同培  史军义 《四川林业科技》2005,26(5):62-63

本文报道了产于中国福建南部的竹亚科一新种.它是单竹属的有节竹.  相似文献   

山核桃水溶性成分中神经营养活性因子的分离与鉴定     

吴建峰  高飞  谢倩  黄坚钦  钱永常 《中国农学通报》2018,34(15):159-164

本研究旨在揭示山核桃水溶性成分的神经营养活性及活性因子。利用脱脂山核桃水溶性提取物处理SH-SY5Y细胞,用倒置相差显微镜观察细胞突触生长,用qPCR检测相关基因表达,使用HPLC 及LC-MS对山核桃水溶性成分神经营养活性因子进行分离鉴定。结果显示,山核桃的水溶性成分能够显著促进SH-SY5Y细胞的突触生长,并可以显著上调SH-SY5Y细胞NF160 基因的表达。HPLC及LCMS检测结果发现其水溶性成分主要组成为胞苷、尿苷、鸟苷、腺嘌呤、腺苷等。由此可知,山核桃水溶性成分通过上调NF160基因表达具有神经营养活性。  相似文献   

A highly sensitive,non‐invasive qPCR‐based strategy for direct quantification of Yersinia ruckeri in fish faeces          下载免费PDF全文

Bikramjit Ghosh  Philip B. B. Crosbie  Barbara F. Nowak  Andrew R. Bridle 《Journal of fish diseases》2018,41(9):1421-1428

Finfish with asymptomatic Yersinia ruckeri infections pose a major risk as they can transmit the pathogen and cause clinical outbreaks in stock populations. Current tools have insufficient quantitative ability for accurately detecting the trace levels of Y. ruckeri typically associated with asymptomatic infection, necessitate invasive or lethal sampling, or require long processing times. This study presents a highly sensitive qPCR‐based method, targeting part of the Y. ruckeri 16S rRNA sequence, that is capable of detecting extremely low levels of Y. ruckeri in noninvasively collected faecal samples. Quantitative precision and accuracy of faecal sample analysis was consistent, despite the complexity of the faecal matrix. The assay demonstrated linearity over a six log‐wide dynamic range. Its limit of detection (LOD) and limit of quantification (LOQ) were 4 and 10 copies of the target sequence, respectively. Sensitivity of the assay was comparable to other qPCR‐based methods without requiring invasive or lethal sampling. Applicability as a screening strategy was tested using passively collected faecal samples. Asymptomatic Y. ruckeri infection was detected in all samples, although none of the fish exhibited overt infection. This method will be beneficial for finfish disease management if developed further as a noninvasive, screening tool against asymptomatic Y. ruckeri infection.  相似文献   

抗马铃薯Y病毒病的VIGS生防剂发酵条件及施用方法优化     

刘天波  蔡海林  曾维爱  魏润洁  刘嘉裕  李帆  周志成  唐前君 《中国农业科技导报》2018,20(11):29-35

病毒诱导的基因沉默（virus-induced gene silence,VIGS）是植物病毒病防治方法之一,能够有效防治马铃薯Y病毒病。以VIGS表达载体发酵液对烟草马铃薯Y病毒病的防治效果为检测指标,筛选最佳发酵液发酵所需的培养基、发酵条件和施用方法。结果表明：VIGS表达载体发酵液最优培养基是YEP培养基;最优发酵条件为装瓶量60 mL/200 mL、初始pH 7.0、培养温度30℃、振荡培养24 h;施用方法以 “原液稀释100倍”、“剪叶后喷施+移栽时灌根”和“含PVY CP和HC-Pro基因片段的VIGS载体发酵液混合使用”的施用效果最佳。上述结果为抗马铃薯Y病毒病的VIGS生防剂发酵提供了理论依据,也为其田间应用打下基础。  相似文献   

Y58S系列组合产量、品质及抗性的综合表现     

扶定  王青林  赵万兵  霍二伟  马汉云  沈光辉  全瑞兰 《中国农学通报》2015,31(3):49-53

为选育不育起点温度低、配合力强、米质优的实用型两系不育系,以近年来新育成的22个Y58S组合为对象,对其产量、品质及病虫抗性进行研究。结果表明：平均产量为8978.7 kg/hm²,所有品质指标均达到《优质稻谷》3级标准的组合占40.9%,感稻瘟病和褐飞虱。因此,Y58S系列组合产量水平较高,稻米品质较优,主要病虫抗性相对较差。  相似文献