用几种电泳方法分析牦牛肝片吸虫不同部位抗原   总被引：1，自引：0，他引：1  

乔代蓉  赵坚 《四川草原》1997,(2):48-51,64

应用ＳＤＳ－ＰＡＧＥ、等电聚焦电泳（ＩＥＦ）及双向电泳三种电泳方法分析牦牛肝片吸虫成虫四种抗原（头抗原－ＨＡ、体抗原－ＢＡ、体表抗原ＳＡ、分泌排泄抗原－ＥＳ）。ＳＤＳ－ＰＡＧＥ结果表明，ＢＡ、ＨＡ、ＳＡ分子量在１２～１００ｋＤ之间，ＥＳ在１２．３～２６ｋＤ之间，蛋白质染色ＢＡ、ＨＡ、ＳＡ、ＥＳ分别显示２５、２４、１８、９条带；ＩＥＦ分析肝片吸虫分别得３４、３３、２８、２２条带，主要由酸性蛋白质组成，主要谱带在ｐＩ４．２０～６．５５内；双向电泳分析ＢＡ、ＥＳ多肽斑点为６９、３０个  相似文献   

投硒后放牧牦牛血硒浓度的变化   总被引：2，自引：1，他引：1  

宋仁德  周玉清  李国梅  圜田立信 《青海畜牧兽医杂志》2003,33(5):9-10

为了解牦牛补硒后血中硒浓度变化,对放牧牦牛按每100kg体重20mg,肌肉注射亚硒酸钠投硒,投药后每间隔1周进行采血,对6周间全血,血浆中硒浓度的变化进行了测定。结果表明:血浆中硒浓度随投硒显著上升(P<0.01),第1周后增至投予前的约3倍,第2周后现最大值(91.46±14.63μg/kg),第3周后开始下降,至4周后降至投前的水平;全血中Se浓度与血浆同样增大,投予后第2周时呈现最大值(129.81±36μg/kg)以后,血中硒浓度至第6周时仍维持107.55μg/kg的高值,约为对照群的3倍,至52周后投硒群牦牛血液中硒浓度仍为对照群的约2倍。试验期间,对照群血液、血浆中硒浓度均无显著变化。  相似文献   

Live-weight gain, apparent digestibility, and economic benefits of yaks fed different diets during winter on the Tibetan plateau   总被引：2，自引：0，他引：2  

Q.M. Dong  X.Q. Zhao  Y.S. Ma  S.X. Xu  Q.Y. Li 《Livestock Science》2006,101(1-3):199-207

Our goal was to determine the effect of diets with different crude protein (CP) contents and metabolizable energy (ME) levels on daily live-weight gain, apparent digestibility, and economic benefit of feedlot yaks on the Tibetan plateau during winter. Yaks were either 2- or 3-years old and randomly selected from the same herd. The 3-year-olds were placed into one of two experimental groups (A and B) and a control (CK₁), and the two-year-olds were placed into one of three experimental groups (C, D and E) and a control (CK₂) (N per group = 5). Yak in the control groups were allow graze freely, while those in the experimental groups yaks were fed diets higher in contains crude protein and metabolizable energy through a winter period inside a warming shed. Results indicated that live-weight gain of treatment groups was higher than their respective controls during experiment, and that daily live-weight gain of every 10 days among different treatments was significant difference (P < 0.05). In addition, apparent digestibility of different diets was linearly and positively related to feedlotting time, and feed conversion efficiency for A, C, D and E groups was quadratically related to feedlotting time (P < 0.01), however, feed conversion efficiency for B group was linearly and positively related to feedlotting time (P < 0.05). The economic benefit was 1.15 for A, 1.89 for B, 1.16 for C, 1.54 for D, and 4.52 for E.  相似文献   

牦牛部分功能基因密码子偏好性的聚类分析     

赵胜  张琴  陈智华  钟金城 《家畜生态》2006,(4)

本文利用系统聚类法对牦牛部分功能基因进行了密码子偏好性的聚类分析,并同普通牛和猪的相关基因作了对比。结果表明:不同物种间,类型或功能相似的基因具有相近的密码子偏好性;三个物种的基因在类型或功能上的差异决定了其密码子使用偏好性的聚类结果;在功能或类型相近的前提下,物种间的差异在一定程度上决定了密码子使用的偏好性。  相似文献   

喷雾干燥牦牛奶粉工艺优化   总被引：1，自引：0，他引：1  

靳义超  闫忠心  李升升  吴海玥 《青海畜牧兽医杂志》2014,44(6):16-17

为对牦牛奶粉喷雾干燥条件进行优化,在单因素实验的基础上,采用L9(34)设计对进风温度,进料速度以及干物质含量进行优化探讨。结果表明:最佳喷雾干燥条件为进风温度为170℃,进料速度为10.4m L/min,干物质含量为8.7g/100g;进风温度对牦牛奶粉的出粉率影响显著(P0.05)。  相似文献   

不同外源性激素对乏情母牦牛处理效果的研究     

陈世彪  许生成  张君 《青海畜牧兽医杂志》2014,(2):11-12

选择420头体况维持在2.0~3.0的当年产犊乏情母牦牛分3个组,用3种不同的方法进行诱导发情处理。第1组:注射GnRH-PG-GnRH;第2组:注射GnRH-PG-E2;第3组:注射PG-GnRH。结果表明:第1组、第2组和第3组经不同的处理后,发情率分别为70.00%(98/140)、54.29%(76/140)、52.14%(73/140),受胎率分别为48.57%(68/140)、34.28%(48/140)、21.43%(30/140)。第1组发情率分别显著高于其它2个组(P0.05);采用GnRH-PG-GnRH处理方案,通过三年扩大试验,发现牦牛平均发情率在66.67%(72/108)以上,受胎率在44.44%(48/108)以上。  相似文献   

Physiological and Biochemical Characteristics of Lactation in Half-lactating Yak     

ZHENG YU-cai  ZHONG Guang-hui  WANG Yong  PENG Xian-wen  MAO Yong-jiang  ZOU Si-xiang  CHEN Wei-hua  CHEN Jie 《中国农业科学(英文版)》2002,1(6):696-701

Yaks of two lactating types, the half-lactating yak and the total lactating yak, were investigated in their milk compositions, milk coagulation properties, and contents of three kinds of hormones, glucose,and protein in plasma. The half-lactating Maiwa yak and Jiulong yak contained significantly higher contents of protein, fat, and activities of alkaline phosphatase and γ-glutamyl transpeptidase in milk than that of the corresponding total-lactating yak breeds, with reduced milk yield and similar lactose level and relative percentages of main milk protein components. The half-lactatin yak resembled yaks in a late stage of lactation in their biochemical composition of milk; however, significant differences were also observed, which indicated that the former was in a special stage of lactation quite different from dairy cows. Milk of the total-lactating or halflactating yaks could be coagulated normally by adding chymosin, with a similar coagulation time. No significant difference was observed between prolactin and progesterone concentrations in plasma of the half-lactating yaks and total-lactating yaks; however, half-lactating yaks had significantly lower level of oestradiol-17 β in plasma than total-lactating yaks.  相似文献   

牦牛和犏牛DDX25/GRTH基因序列及其在睾丸组织的表达水平     

张思源  柴志欣  彭娅林  钟金城 《西北农业学报》2015,24(10):1-9

为了解DDX25基因的结构、功能及对犏牛雄性不育的影响。采用RT-PCR技术克隆获得牦牛和犏牛的DDX25基因,对核苷酸序列和氨基酸序列进行生物信息学分析,运用实时荧光定量PCR技术检测睾丸组织DDX25 mRNA的表达情况。结果表明,牦牛和犏牛DDX25基因编码区序列全长均为1 452bp,编码483个氨基酸,氨基酸存在19个磷酸化位点,无信号肽。牦牛睾丸组织DDX25基因表达水平显著高于犏牛,DDX25基因在犏牛睾丸组织的低表达与其雄性不育存在一定关系,该基因可作为研究犏牛雄性不育的候选基因。  相似文献   

冷季补饲对那曲牦牛生长性能和血液生化指标的影响   总被引：1，自引：0，他引：1       下载免费PDF全文

刘秀  沙玉柱  张伟  胡江  左志  石斌刚  赵志东  张成福 《中国牛业科学》2021,47(3):30-33

[目的]为明确高寒牧区牦牛冷季补饲效果。[方法]本试验通过测定日增重和采集血液去分析补饲精饲料对不同年龄那曲牦牛生长速度和血液生化指标的影响。[结果]研究表明,2岁、3岁牦牛试验组和对照组的日增重均显著高于1岁、4岁(P0.05);在不同年龄间,牦牛各个年龄的试验组日增重均高于对照组,但1岁、2岁试验组极显著高于对照组(P0.01),3岁、4岁试验组日增重显著高于对照组(P0.05)。不同年龄阶段淀粉酶(AMY)、尿素氮(BUN)、尿素(UREA)差异极显著(P0.01),钙(Ca)、磷(P)和尿素氮/肌酐差异显著(P0.05)外,其余指标差异均不显著(P0.05),同一年龄的牦牛在不同的饲养管理水平下,各项生化指标中补饲牦牛的淀粉酶(AMY)、尿素氮(BUN)、尿素(UREA)极显著高于放牧牦牛(P0.01),钙(Ca)和尿素氮/肌酐显著高于放牧牦牛(P0.05),磷(P)显著低于放牧牦牛(P0.05)。[结论]表明冷季补饲有效补充了那曲牦牛的机体能量,为其机体冷季正常生长发育提供了动力,从而提高了其越冬能力和适应性。  相似文献   

青海高原牦牛肩胛上、下神经和腋神经解剖     

常兰 《动物医学进展》1999,(1)

本文报道了生活在高寒低氧环境下的青海高原牦牛的肩胛上、下神经和腋神经的起源、位置、行程、分支和分布情况。其主要特征是肩胛上神经、肩胛下神经纤维都来自第６、第７颈神经的腹侧支、其神经的分支支配与牛、水牛、马的基本相同。腋神经纤维来自第７、第８颈神经和第１胸神经,其分支支配三角肌、小圆肌、冈下肌、臂头肌锁臂部以及前臂的筋膜和皮肤  相似文献