Physiological processes associated with salinity tolerance in an alfalfa half‐sib family          下载免费PDF全文

M. Rokebul Anower  M. D. Peel  I. W. Mott  Y. Wu 《Journal of Agronomy and Crop Science》2017,203(6):506-518

We previously reported an alfalfa half‐sib family, HS‐B, with improved salt tolerance, compared to parental plants, P‐B. In this study, we conducted additional experiments to address potential physiological mechanisms that may contribute to salt tolerance in HS‐B. Vegetatively propagated HS‐B and P‐B plants were treated with a nutrient solution (control) or a nutrient solution containing NaCl (EC = 12 dS/m). Shoots and roots were harvested at various time points after treatment for quantification of proline, soluble sugar, and H₂O₂ using spectrophotometers. Subcellular localization and quantification of Na in roots were conducted using a Na⁺‐specific dye under a confocal microscope. HS‐B produced 86 and 89% greater shoot and root dry biomass, respectively, compared to parental plants, P‐B, under salinity in the greenhouse. Under saline conditions the HS‐B shoots accumulated 115% and roots 55% more soluble sugars than P‐B counterparts. The non‐saline HS‐B shoots, however, showed 72% less soluble sugars than the non‐saline P‐B plants. Under saline conditions HS‐B accumulated 39% less proline in shoots, while roots accumulated 56% more proline, compared to their P‐B parents. HS‐B plants also showed a greater reduction of stomatal conductance under mild saline stress. HS‐B shoots and roots contained 3–4 times less reactive oxygen species (H₂O₂) after salt treatment compared to P‐B plants. Sodium localization and distribution analysis using Na⁺‐specific dye revealed HS‐B plants accumulated 88% and 48% less Na⁺ in stele and xylem vessels compared to P‐B. The study provides insights into the potential mechanisms that may contribute to salt tolerance in HS‐B: maintaining RWC by accumulating soluble sugars while reducing transpiration, maintaining healthy status by reducing oxidative stresses, and preventing salt toxicity by reducing accumulation of Na⁺ inside root cells and xylem.  相似文献   

Effect of Alfalfa Flavonoids on Growth Performance and Serum Indexes of Sheep     

WANG Meng-zhu  LIU Yan-feng  WANG Wen-qi  NIE Cun-xi  TANG Shu-zhen  ZHANG Wen-ju 《中国畜牧兽医》2015,42(9):2345-2351

This experiment was conducted to evaluate the effect of alfalfa flavonoids (Medicago sativa) on growth performance and serum indexes of sheep.This experiment was performed with hybrid F1 sheep of Suffolk sheep×Small-tailed Han sheep.Twenty-eight sheep with the body weight of (27.02±3.03) kg were randomly divided into 4 groups with 7 replicates in each group and 1 sheep per replicate.Sheep in different groups were fed diets with 0 (group Ⅰ), 0.1% (group Ⅱ), 0.2%(group Ⅲ) and 0.4% (group Ⅳ) alfalfa flavonoids, respectively.The experiment was cosisted of 7-day-preliminary trial and 41-day-formal trial.The results showed as follows:① Dietary supplementation of alfalfa flavonoids had no significant effects on growth performance of sheep(P>0.05).② Dietary supplementation of alfalfa flavonoids had significant effects on the contents of GLB, ASP, BUN, HDL and P in serum (P<0.05).Serum GLB content at the 1st day in group Ⅲ was significantly lower than that in groupsⅠand Ⅱ(P<0.05), serum ASP contents at the 1st and 21th days in group Ⅳ were significantly lower than those in group Ⅰ(P<0.05), serum BUN content at the 1st day in group Ⅳ was significantly lower than that in group Ⅰ(P<0.05), serum HDL content at the 21th day in group Ⅱ was significantly lower than that in groups Ⅰ and Ⅲ (P<0.05), serum P content in group Ⅳ at the 21th day was significantly lower than that in groups Ⅱ and Ⅲ (P<0.05).In conclusion, dietary supplementation of alfalfa flavonoids might modulate lipid metabolism, improve nitrogen utilization, maintain energy metabolic and balance Ca and P contents, and have the potential function for promoting growth and no adverse effect on liver.The results suggested that the appropriate supplemental level of alfalfa flavonoids for sheep was 0.2%.  相似文献   

西北旱区喷灌条件下紫花苜蓿生长特征与品质指标的关系   总被引：1，自引：0，他引：1  

李岩  苏德荣  李宏韬 《草业学报》2018,27(10):54-65

西北旱区由于独特的气候条件,生产的苜蓿干草品质好,产量高。如何根据苜蓿的田间指标估测干草品质参数,对于苜蓿草产品生产销售具有重要意义。研究在喷灌条件下不同灌溉量对紫花苜蓿的生长品质的影响,并利用生长指标对紫花苜蓿的品质进行预测。试验于2014至2015年于中国农业大学石羊河流域生态节水试验站进行为期2年的田间试验。试验共设3个灌溉处理:A₁灌溉量为100%紫花苜蓿蒸腾蒸散量(ETc),A₂,A₃的灌溉量分别为A₁处理的66%,33%,以及一个不灌溉处理A₄。结果表明,苜蓿生长指标如株高(H)和茎叶比(SLR)随生育期的推进呈上升趋势,而鲜干比(FDR)呈下降趋势;所有生长指标随着灌溉量的减少均呈下降趋势。在品质方面,随着生育期的推进,所有处理的粗蛋白(CP)呈下降趋势,而中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)则呈现上升趋势;随着灌溉量的降低,NDF与ADF呈下降趋势,而CP则呈上升趋势。各生长指标与品质指标的拟合结果表明,H和SLR均与各品质指标显著线性相关(P<0.001),且H与品质拟合结果相关系数最高(R²>0.900),而FDR则与各品质指标不显著。这些结果表明,在田间管理较好,长势良好,杂草与病虫害发生较少的情况下,可以用株高对苜蓿干草品质做出预测。  相似文献   

Use of Porcine Meal in Plant‐based Practical Diets for Pacific White Shrimp,Litopenaeus vannamei          下载免费PDF全文

Xuan Qiu  D. Allen Davis 《Journal of the World Aquaculture Society》2018,49(3):582-589

Two growth trials were conducted to evaluate and confirm the efficacy of a porcine meal (PM) with high protein content (>90%) as an alternative feed ingredient in commercial‐type feed formulation for Pacific white shrimp, Litopenaeus vannamei. Six experimental diets were formulated for the two growth trials. The first five diets contained increasing levels (0, 1, 2, 4, and 6%) of PM as a replacement for soybean meal in a plant‐based diet with low inclusion level (6%) of fish meal (FM). The last experimental diet was produced utilizing 4.2% PM to completely replace FM. In Trial 1, shrimp (1.5 g initial mean weight, 20 shrimp/tank, n = 4) were offered test diets for 6 wk in a semirecirculation system. At the end of Trial 1, shrimp fed with the diet containing 6% PM exhibited significantly enhanced weight gain (WG), feed conversion ratio (FCR), and survival compared to those fed with the diet devoid of FM. As survival was poor across all treatments and different densities could mask growth results the trial was repeated. In Trial 2, shrimp (0.85 g, 15 shrimp/tank, n = 4) were offered diets for 6 wk. Dietary supplementation of PM at 6% significantly improved WG, FCR, and apparent net protein retention in contrast with the treatment devoid of FM, confirming the same trends in Trial 1. No significant difference was detected in protein, lipid, moisture, and mineral profiles of whole‐body shrimp as well as survival across all the treatments. Results of this study indicate that PM is a good high protein source in shrimp feeds, which can be included up to 6% in the low FM‐based diet without compromising the growth of shrimp.  相似文献   

Feeding native laying hens diets containing palm kernel meal with or without enzyme supplementations: 1. Feed conversion ratio and egg production     

《The Journal of Applied Poultry Research》2011,20(1):40-49

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Early interspecific dynamics,dry matter production and nitrogen use in Kernza (Thinopyrum intermedium) – alfalfa (Medicago sativa L.) mixed intercropping     

Shoujiao Li  Ana Barreiro  Erik Steen Jensen  Yingjun Zhang 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2020,70(2):165-175

ABSTRACT

The global interest in growing perennial grain crops such as intermediate wheatgrass (Thinopyrum intermedium) (Kernza) for production of food and feed is increasing. Intercropping Kernza with legumes may be a sustainable way of supplying nitrogen to soil and associated intercrop. We determined the competitive interactions between intercropped Kernza (K) and alfalfa (Medicago sativa L.) (A) under three inorganic nitrogen (N) rates N0, N1, N2 (0, 200, 400 kg ha^?1) and five species relative frequencies (SRF) (100%K:0%A, 75%K:25%A, 50%K:50%A, 25%K:75%A and 0% K:100%A) in mixed intercrops (MI) in a greenhouse pot experiment. After 11 weeks of growth. Kernza dry matter yield (DM) and N accumulated (NACC) were low, but alfalfa DM and NACC high at 0 kg N ha^?1. 200 and 400 kg N ha^?1 fertiliser application increased the competitive ability (CA) of Kernza and reduced the CA of alfalfa. SRF had large impacts on alfalfa DM, NACC and NFIX only at 0 kg N ha^?1 fertiliser, and insignificant impacts on Kernza at all N fertiliser levels, indicating that adjustment of SRF may not be an effective way to modulate the interspecific competition of Kernza. Further research on the other factors that influence the interspecific competition are warranted.  相似文献   

黔育1号菊苣与紫花苜蓿、鸭茅间作栽培效果研究     

覃涛英  付薇  苏生  陈伟  韩永芬  李德芳  马宁  马培杰 《畜牧与饲料科学》2019,40(7):55-59

依据贵州省松桃县的气候特点和牧草种植需要,用黔育1号菊苣与北林202紫花苜蓿、黔草4号鸭茅分别进行间作试验,从产量、营养品质、生产效益等方面对间作复合群体与3种牧草单播进行比较分析,探讨黔育1号菊苣与不同牧草间作种植模式的增产效益。结果表明,在贵州松桃地区,黔育1号菊苣+北林202紫花苜蓿间作,年均鲜草产量可达87.27 t/hm²,比紫花苜蓿单播产量增加36.17%,生产效益提高20.77%,粗蛋白含量达26.83%。该研究结果可为松桃县种草养畜产业的发展提供技术支撑。  相似文献   

河西荒漠灌区紫花苜蓿施肥效应及其基于数据包络分析法的经济效益研究     

吴勇  刘晓静  蔺芳  童长春 《草业学报》2020,29(9):94-105

为科学准确地评判河西走廊苜蓿主产区紫花苜蓿饲草生产中施肥措施对其产品质量及生产收益的影响,本研究以“甘农3号”紫花苜蓿为材料,通过2016、2017年2年田间试验,以该区域紫花苜蓿饲草生产的平衡施肥推荐方案(N 103.5 kg·hm^-2、P₂O₅ 105 kg·hm^-2、K₂O 90 kg·hm^-2)为对照,探讨了不施肥及3种不完全施肥(缺氮偏施、缺磷偏施、缺钾偏施)处理下紫花苜蓿的生产性能,并采用数据包络分析法(DEA)对其经济效益进行分析。结果表明:与不施肥相比,施肥措施各处理均显著提高紫花苜蓿产量、蛋白总量,降低其酸性洗涤纤维和中性洗涤纤维,提高了相对饲用价值,从而改善了紫花苜蓿品质,并有效增加了经济效益;与氮、磷、钾平衡施肥相比,各偏肥处理的紫花苜蓿产量和品质均显著低于平衡施肥,尤以缺磷偏施的降幅最大,2016、2017年2年的产量和蛋白总量降幅分别达到25.9%、25.7%和33.4%、33.1%。因此,磷是河西荒漠灌区紫花苜蓿饲草生产的养分限制因子,氮、磷、钾对该地区紫花苜蓿生产性能影响顺序为:磷>氮>钾。运用数据包络分析法(DEA)分析出河西荒漠灌区紫花苜蓿的施肥效应为氮、磷、钾平衡施肥的经济效益最优,为DEA有效;不完全施肥的3个评价单元及不施肥评价单元为DEA无效,其中,不施肥经济效益最低,3个不完全施肥评价单元中的缺磷偏肥的紫花苜蓿经济效益比缺氮偏肥和缺钾偏肥更低;另外还以DEA模型推算出不同施肥措施下紫花苜蓿饲草生产经济效益改进的具体方案,其中,不施肥的紫花苜蓿饲草生产需调整的幅度最大,调整额度达10678.88 CNY·hm^-2,各施肥措施需调整的幅度排序为:不施肥>缺磷偏施>缺氮偏施>缺钾偏施。  相似文献   

Effect of dietary Moringa stem meal level on growth performance,slaughter performance and serum biochemical parameters in geese     

Shuangshuang Zhai  Miaomiao Li  Mengmeng Li  Xiufen Zhang  Hui Ye  Zhenping Lin  Wence Wang  Yongwen Zhu  Lin Yang 《Journal of animal physiology and animal nutrition》2020,104(1):126-135

Moringa stem meal (MSM) with a high level of crude fibre (CF) might be developed and utilized in herbivorous geese as an unconventional feedstuff. The aim of this study was to investigate the effect of the MSM level in the diet on the growth performance, slaughter performance, breast meat quality and serum biochemical parameters in geese from 22 to 70 days of age. A one-factor completely randomized design was adopted in our study. A total of one thousand eight 21-day-old geese were randomly divided into six groups, with six replicates per group and 28 birds per replicate. The geese were fed diets containing MSM levels of 0, 20, 40, 60, 80 or 100 g/kg during day 22–70. The dietary MSM level had no effect (p > .05) on the final body weight (BW), average daily gain (ADG) or average daily feed intake (ADFI). The feed/gain ratio (F/G) increased linearly (p < .001) as the dietary MSM level increased. No differences (p > .05) were observed in the slaughter performance, meat quality and the relative organ weight (except for thymus) of the geese (p > .05). The relative weight of the thymus in the geese fed diets with supplementation of MSM was higher than that in the non-supplemented MSM control group (p < .05). In addition, 100 g MSM/kg of diet decreased the serum glucose (GLU) level (p < .05) and increased the alanine transaminase (ALT) enzyme activity (p = .03). Dietary MSM levels of no more than 60 g/kg had no effects on the growth performance and slaughter performance, whereas diets with 100 g MSM/kg increased the F/G and serum ALT enzyme activity, as well as decreasing the serum GLU level. Therefore, MSM provided at a reasonable level could be developed as an unconventional feedstuff for geese at the finisher period.  相似文献   

呼伦贝尔地区苜蓿人工草地CO_2通量变化特征     

徐丽君  徐大伟  杨桂霞 《草原与草坪》2015,(5):60-64

试验采用密闭箱技术对呼伦贝尔地区种植的苜蓿人工草地进行碳通量研究,采用反硝化-分解作用模型(DNDC)进行模拟。试验结果表明:对苜蓿人工草地CO2通量24h连续观测发现,CO2通量全天变化的平均值(238.15mg/(m2·h)与11∶00(245.32mg/(m2·h)的通量变化值接近,试验从早上9∶00~11∶00进行温室气体的采集方法合理、可行;在整个生长季变化表明,7、8月CO2通量变化较显著(P0.05),且受到降水的影响较大,伴随降水的发生,CO2通量会发生不同幅度的变化,土壤温度和含水量对CO2通量有一定的影响(r0.45,P0.05);通过DNDC模型的模拟,能较好的反映苜蓿人工草地CO2通量的变化情况,但模拟结果数值普遍有些偏高。  相似文献