Cryopreservation of Sheep Red Blood Cells: 4. Titrations of The First Complement Component with Frozen Eac4 Cells     

Veslemy Myhruold 《Acta veterinaria Scandinavica》1980,21(2):291

The cryoprotection of the sheep erythrocyte intermediate EAC4 cells, used as reagent in titration of the first complement component, Gl, was investigated. The cryoprotective agents tested were untreated polyvinylpyrrolidone (PVP), purified PVP, neutralized PVP and a hydroxyethylated potato starch of high viscosity, Avelex 1030, hydrolyzed for 40 min. Recovery of EAG4 cells after thawing was 80–90 %, with best results using untreated, purified or neutralized PVP. The EAG4 cells frozen in the presence of untreated PVP showed, however, increased susceptibility to the hemolytic action of Gl, whereas cells frozen with purified or neutralized PVP or with Avelex 1030 gave titers similar to that obtained with fresh cells. Gl titrations with frozen and thawed EAG4 cells gave more reproducible results than those obtained when titrations were performed with fresh separately prepared cells.  相似文献   

Protein polymorphism of an isoform of the third complement component in the common carp Cyprinus carpio     

Miki  NAKAO  Masakazu  KONDO  Tomoki  YANO 《Fisheries Science》2004,70(3):366-371

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Comparative evaluation of kinetic nephelometry for antibody detection     

O.R Kaaden  B Frenzel  V Moennig 《Comparative immunology, microbiology and infectious diseases》1979,2(4):477-484

The sensitivity of the nephelometry performed as kinetic and end-point measurement for the detection of serum antibodies was evaluated in an experimental system and compared with that of the complement fixation and single radial immunodiffusion tests. The corresponding antibody titres of the used anti-FCS antiserum with BSA antigen were estimated to be 2⁻⁷ in complement fixation, 2⁻⁶ in single radial immunodiffusion and 2⁻⁴ in kinetic (rate) nephelometry tests. From the described findings it was concluded that the kinetic nephelometry with its present technical instrumentation provided a rapid serological technique to establish quantitative Heidelberger curves under experimental conditions but did not meet the required sensitivity for the detection of naturally occurring antibodies in infected donors.  相似文献   

Bovine adenoviruses—IV. Two mixed antigens for routine serodiagnosis by complement fixation reaction     

A Messner  B Hinaidy  F Bürki 《Comparative immunology, microbiology and infectious diseases》1979,1(4):351-359

The frequency of bovine adenovirus infections occurring in cattle has been grossly underestimated up to the present day. Primary isolation of serotypes belonging to bovine subgroup II is cumbersome. Hardly any laboratory has used all 8 officially-recognised bovine serotypes in seroneutralisation-tests, which should be done when this type-specific method is used for serodiagnosis. The complement fixation test, known as group-reactive from human adenovirus serology, has failed to disclose the true incidence of bovine infections, as until recently the importance of a novel additional group-reactive bovine adenovirus antigen had been undisclosed. Here we describe production, composition and performance of two mixed antigens for complement fixation reactions, which take into account recent findings by our team on peculiarities of bovine adenovirus antigens. Mixed antigen 1 contains bovine serotypes 1, 2 and 3 and detects group-specific antibodies of the classical mastadenoviruses. Mixed antigen 2 contains bovine serotypes 4, 5, 6, 7 and 8 and determines group-specific antibodies against the novel paramastadenoviruses. In addition, each antigen is capable of demonstrating complement-fixing type-specific antibodies in sera against the respective types included in each mixed antigen, with a net enhancing effect produced by mixing. Use of the 2 mixed antigens promptly shows whether bovine adenoviruses, presently recognised as well as unclassified, are involved in a given outbreak of respiratory or enteric disease of cattle. If needed, the responsible type may be determined in a second step of serological investigation.  相似文献   

Serologic response to Brucella abortus of cattle and guinea pigs experimentally inoculated with a Yersinia enterocolitica serotype from milk     

K Nielsen  G M Ruckerbauer  B S Samagh 《Comparative immunology, microbiology and infectious diseases》1981,4(1):59-64

Ten strains of Yersinia enterocolitica belonging to ten various serogroups isolated from raw milk were inoculated into groups of five guinea pigs and five calves. Y. enterocolitica serotype 0:16 was the only serotype tested that induced an antibody response to Brucella abortus in calves. No anti-Brucella response could be demonstrated serologically in guinea pigs. Activity of the anti-Y. enterocolitica 0:16 calf sera against B. abortus antigen was shown by the tube agglutination test, and by the complement fixation test. The early agglutinating antibody response was partly sensitive to reduction by 2-mercaptoethanol. This sensitivity decreased later in the response. This is the first report of anti-Brucella responses induced by a serotype of Y. enterocolitica other than 0:9; sera from a group of five calves inoculated with 0:9 were tested by the same serological techniques for comparison.  相似文献   

东亚夏季气候可预测性的一个论证     

蔡尔诚  许春姝  智协飞 《黑龙江八一农垦大学学报》2002,14(3):5-8

本文从冬季东亚西风急流与夏季副热带高压之间的动能互补与平衡关系出发,论证了夏季气候特征的可预测性在0.80以上,平均每10年(次)预测中可做到8年以上基本趋势正确。  相似文献   

金线莲多糖对小鼠免疫功能的影响     

许丹妮  马玉芳  李健  黄一帆 《福建农业大学学报(自然科学版)》2013,(5):536-538

将40只18-22g昆明雄性小鼠随机分为空白对照组和金线莲多糖大、中、小3个剂量组,分别灌胃蒸馏水和200、100、50mg·kg^-1多糖,连续灌胃20d,第21天时,摘小鼠眼球采血,通过检测血清免疫球蛋白（IgG、IgM、IgA）和补体（C3、C4）的含量,研究金线莲多糖对小鼠免疫功能的影响．结果表明：金线莲多糖各剂量组小鼠的IgA含量与空白对照组比较无显著差异（P〉0.05）;IgM含量与空白对照组比较均显著提高（P〈0.05）;大、中剂量组的IgG含量与空白对照组比较显著提高（P〈0.05）;大剂量组的C3,含量与空白对照组比较极显著提高（P〈0.01）;各剂量组的C。含量与空白对照组比较均显著提高（P〈0.05）．上述结果表明,金线莲多糖在一定程度上能提高小鼠的免疫功能．  相似文献   

皮质醇对黄颡鱼成鱼吞噬细胞和补体活性的影响     

田珍  刘小玲  丁益群  刘旭博 《现代农业科技》2009,(9):232-233

采用皮质醇混饲投喂的方法．模拟自然条件下的慢性应激,研究皮质醇对黄颡鱼咸鱼吞噬细胞呼吸暴发和血清补体旁路溶血活性（ACH50）的影响。研究结果与结论如下。皮质醇混饲投喂可使血清皮质醇浓度持续显著升高,各周对照组血清皮质醇含量均无显著性差异,证明采用皮质醇混饲投喂的方法可模拟自然慢性应激情况下的血清皮质醇浓度持续显著升高。从第2周开始头肾吞噬细胞的呼吸暴发功能显著下降（P〈0．05）,证明皮质醇对头肾吞噬细胞的杀菌能力产生显著抑制作用。10mg／kg剂量组,ACH50在投喂第2、第5、第6周显著低于对照组（P〈0．05）,100mg／kg剂量组,ACH50从第2周开始一直都极显著低于同期对照组（P〈0．01）,证明皮质醇显著抑制血清补体旁路溶血活性,且血清皮质醇浓度越高其抑制作用越强。  相似文献   

微耕机耕深辅助控制系统的设计和试验研究   总被引：2，自引：0，他引：2  

何家慧  康杰  聂友红  何培祥  李云伍  赵华慧 《农机化研究》2016,(3):119-123

在农业生产中,耕深的稳定性是评价耕作质量的重要指标。为此,结合目前微耕机耕深不稳定的现状,设计了一种耕深辅助控制系统,主要用于辅助控制微耕机的耕作深度,提高微耕机的耕作质量。该系统主要由耕深检测装置、控制装置、执行装置和显示界面组成。检测装置用来间接检测耕深,控制单元对检测到的信号进行分析后控制电机转动,从而间接控制耕深调节阻力杆的上下移动,以实现耕深辅助调节。显示模块用于显示实时耕深,为操作人员提供参考。田间试验结果表明:耕深稳定在120~130mm时的耕深稳定性变异系数为5.82%,满足农艺要求。所提出的耕深测量方法、辅助控制执行装置和显示界面对微耕机设计具有一定的参考意义。  相似文献   

A site index model for lodgepole pine (Pinus contorta Dougl. var. latifolia) in northern Sweden     

Mateusz Liziniewicz  Urban Nilsson  Eric Agestam  Per Magnus Ekö  Björn Elfving 《Scandinavian Journal of Forest Research》2016,31(6):583-591

A model was developed for predicting site index (SI) values and top height growth of lodgepole pine in northern Sweden. Data on 169 experimental plots from 61 experiments were used for model construction. Six dynamic site equations derived using the generalized algebraic difference approach (GADA) were tested. All of the equations estimate height and SI values on the basis of a known height and total age, and are base-age invariant. Unbiased parameter estimates were obtained by non-linear modelling without accounting for autocorrelation of residuals. The special formulation of the Hossfeld model and the GADA-transformed logistic function produced the most reliable SI curves. The Hossfeld model is recommended as it did not produce extreme outlying estimates for young stands. The developed model is polymorphic with variable asymptotes and can predict SI and dominant height growth for lodgepole pine between 20 and 50 years old.  相似文献