夏秋反季节莴苣的营养吸收规律初探   总被引：2，自引：0，他引：2  

刘毅  李桂莲  文林宏  王天文  钱晓刚 《贵州农业科学》2006,34(6):46-48

对夏秋反季节莴苣营养吸收规律的试验结果表明,莴苣的生物量随莴苣的生长进程逐渐增加。移栽45 d后干物质积累量占全生育期的80%以上;氮、磷、钾的吸收最高峰出现在移栽后的45~60 d,吸收强度分别达22.47 mg/株.d、8.01 mg/株.d和35.61 mg/株.d。  相似文献   

大丽轮枝菌粗毒素在棉花根冠细胞生物学测定方法上的研究     

范尉尉  陈霞  马春红  李广敏  张香云  耿军义 《华北农学报》2006,21(1):55-58

在棉花根冠细胞生物测定过程中,棉花黄萎病病菌通过提纯复壮而达到较强的侵染能力,并制备出较多的黄萎病菌粗毒素。介绍了粗毒素的简易制备、根冠细胞培养与制备过程以及染色过程中染色剂的选择、染色时间的长短。实验与传统方法相比有几点改进:总结出细胞振荡时间在1~2 min之内;之后黑暗条件下静置培养2~6 h;选用pH为6.5的0.01%中性红染色3~5 min,之后加入一滴0.2%伊文思兰染色,伊文思兰的染色时间应严格掌握在3min以内等,为根冠细胞测定方法的推广提供了一定的理论支持。  相似文献   

Antiplatelet and Antithrombotic Effects of Isaridin E Isolated from the Marine-Derived Fungus via Downregulating the PI3K/Akt Signaling Pathway     

Ni Pan  Zi-Cheng Li  Zhi-Hong Li  Sen-Hua Chen  Ming-Hua Jiang  Han-Yan Yang  Yao-Sheng Liu  Rui Hu  Yu-Wei Zeng  Le-Hui Dai  Lan Liu  Guan-Lei Wang 《Marine drugs》2022,20(1)

Isaridin E, a cyclodepsipeptide isolated from the marine-derived fungus Amphichorda felina (syn. Beauveria felina) SYSU-MS7908, has been demonstrated to possess anti-inflammatory and insecticidal activities. Here, we first found that isaridin E concentration-dependently inhibited ADP-induced platelet aggregation, activation, and secretion in vitro, but did not affect collagen- or thrombin-induced platelet aggregation. Furthermore, isaridin E dose-dependently reduced thrombosis formation in an FeCl₃-induced mouse carotid model without increasing the bleeding time. Mechanistically, isaridin E significantly decreased the ADP-mediated phosphorylation of PI3K and Akt. In conclusion, these results suggest that isaridin E exerts potent antithrombotic effects in vivo without increasing the risk of bleeding, which may be due to its important role in inhibiting ADP-induced platelet activation, secretion and aggregation via the PI3K/Akt pathways.  相似文献   

重庆三峡库区加工甜橙园土壤养分状况分析     

习建龙  彭良志  淳长品  朱春钊  杨惠栋  王男麒  江才伦  凌丽俐 《中国果业信息》2015,44(1)

为了解重庆三峡库区加工甜橙园土壤养分含量状况,在重庆7县（区）随机选取132个果园,分析背景土壤和农化土壤pH值、有机质和有效态矿质养分含量。结果显示,分别有56.8%和60.8%的果园背景土壤和农化土壤pH值适宜甜橙生长,农化土壤酸性土比例增加;背景土壤和农化土壤有机质含量不足（<15 mg?Kg-1）的比例分别为77.3%和85.3%,碱解氮处于适宜范围的比例分别为56.8%和61.5%,有效钙过剩（高量和过量）的比例分别为79.6%和76.7%;背景土壤有效磷、钾不足(缺乏和低量)的比例分别为95.4%和72.8%,有效锌、硼不足的比例分别为54.5%和70.5%;农化土壤有效磷、钾不足的比例分别为60.8%和57.6%,有效锌、硼不足比例分别为65.9%和90.7%。背景土壤和农化土壤有效镁、铁、锰及铜不足和过量并存。总体而言,土壤养分状况中等偏差;与背景土壤相比,农化土壤pH值、有机质、有效钙、锌和硼含量下降且缺锌严重,其它养分含量则有所上升。  相似文献   

松嫩平原黑土区施肥与土壤速效钾累积关系的研究     

富东英  张清  侯中田 《黑龙江八一农垦大学学报》2006,18(5):30-33

为研究在固定施肥模式下速效钾的累积量的变化,采用回归旋转组合设计实施试验,建立了松嫩平原黑土区土壤速效钾与农肥,化肥和秸秆为实验因子的数学模型,结果表明,要使每667m2速效钾保持在适宜的230～270mg/kg水平上,应在5年中施用农肥832～1121kg,化肥34～947.8kg,秸杆93～122kg。  相似文献   

仔猪黄痢K88-K99-987p-F41多价菌毛疫苗的制备及小鼠免疫试验   总被引：2，自引：0，他引：2  

李鹏  王家乡  程碧军 《动物医学进展》2006,27(9):55-58,96

为了更好地预防仔猪黄痢,选取野生分离菌株HN2001（K88ab）、HN2002（K88ac）、HN2003（K88ad）、HN2004（K99）、HN2005（987p）和HN2006（F41）培养后用低温磁力搅拌法提取菌毛,制成5批多价菌毛混合油乳剂灭活苗。试验结果表明,5批多价灭活疫苗对小鼠的平均保护率达95．0％,为进一步进行本体动物试验提供了有价值的参考资料。  相似文献   

长期秸秆还田和地下水位对土壤镉积累及有效性的影响   总被引：1，自引：0，他引：1  

吴佳琪  黄运湘  尹力初  梁玉文  黄玲  向艳艳  施强 《农业环境科学学报》2020,39(9):1957-1963

以1982年开始的长期定位试验为对象,研究了2个地下水位(高水位-20 cm和低水位-80 cm)下3种施肥方式(高量秸秆还田HS、常量秸秆还田MS、化肥CF)对水稻土镉积累及有效性的影响。结果表明:高水位条件下,长期秸秆还田可增加土壤总镉及有效态镉含量,HS和MS处理分别较CF处理提高21.8%、59.9%和9.8%、49.2%。低水位条件下,HS处理土壤总镉及有效态镉含量高于CF处理,较CF处理分别提高11.2%和31.6%; MS处理土壤总镉及有效态镉含量低于CF处理,较CF处理分别下降8.8%和14.3%。2012年,在保证原定位试验有足够重复的前提下,变更高水位条件下的部分试验处理。当HS处理变更为CF处理5 a后,土壤总镉和有效态镉含量分别较变更前下降2.5%和5.7%;CF处理变更为MS处理后,土壤总镉和有效态镉含量分别较变更前增加16.5%和58.9%。相同施肥处理,高水位土壤总镉含量高于低水位土壤。在MS处理下,高水位土壤有效态镉含量也高于低水位土壤,而在HS和CF处理下,低水位土壤有效态镉含量高于高水位土壤。土壤镉含量和土壤基本理化性质的相关分析表明,土壤总镉与土壤有机质、络合态铁含量极显著正相关,与pH显著负相关;土壤有效态镉与土壤pH、游离氧化铁极显著负相关。研究表明,施肥方式和地下水位是通过改变土壤的基本性质影响土壤镉的积累及其有效形态。  相似文献   

The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2          下载免费PDF全文

ZHANG Ya-ni  HU Cai  WANG Ying-jie  ZUO Qi-sheng  LI Bi-chun 《农业科学学报》2020,19(12):3054-3064

  相似文献   

氮磷钾用量对小麦冠层不同层次光截获和干物质分配的影响     

魏庆薪  于振文  张永丽  石玉 《麦类作物学报》2020,40(11):1351-1356

为明确不同氮、磷、钾用量对小麦冠层不同层次光截获和干物质分配的影响,以济麦22为供试材料,设置F0（不施肥）、F1（N 180 kg·hm^-2,P₂O₅ 75 kg·hm^-2,K₂O 60 kg·hm^-2）、F2（N 225 kg·hm^-2,P₂O₅ 120 kg·hm^-2,K₂O 105 kg·hm^-2）和F3（N 270 kg·hm^-2,P₂O₅ 165 kg·hm^-2,K₂O 105 kg·hm^-2）4个施肥量处理,比较分析开花后不同氮、磷、钾用量对小麦叶面积指数、冠层不同层次光截获特性和成熟期干物质分配的影响。结果表明,F1处理下叶面积指数显著高于F0处理,而与F2和F3处理间无显著差异;开花后15 d,F1处理下小麦冠层不同层次及总PAR截获率和截获量均显著高于F0处理,而与F2和F3处理间无显著差异。F1处理下成熟期干物质在小麦冠层不同层次营养器官中的分配量、籽粒中的分配量及总干物质积累量显著高于F0处理,而与F2和F3处理间无显著差异。成熟期干物质在小麦冠层不同层次营养器官和籽粒中的分配量以及总干物质积累量与冠层上层（顶部至株高2/3）、中层（株高2/3至株高1/3）和总PAR截获率均呈显著正相关。F1处理（N 180 kg·hm^-2,P₂O₅ 75 kg·hm^-2,K₂O 60 kg·hm^-2）为本试验条件下的最优处理。  相似文献   

ROCK promotes high glucose-induced cardiomyocyte apoptosis by inhi-biting PI3K/Akt signaling pathway     

BAI Xue  YU Hui  ZHU Ju-ru  HAN Zhong-li  YANG Xiao-min 《园艺学报》2020,36(5):788-795

AIMTo investigate whether Rho-associated coiled-coil kinase (ROCK) is involved in high glucose-induced apoptosis of primary cardiomyocytes by regulating PI3K/Akt signaling pathway. METHODSPrimary Wistar rat cardiomyocytes were cultured and identified by α-sarcomeric actin (α-SCA) immunohistochemistry. Cardiomyocytes were treated with 5.5, 33 and 40 mmol/L glucose for 48 h. The cell viability was measured by MTT assay, and the mRNA expression of ROCK1 and ROCK2 in the cardiomyocytes was detected by RT-qPCR. Flow cytometry was used to analyze the apoptosis of the cardiomyocytes. The protein levels of ROCK1, ROCK2, cleaved caspase-3, Bcl-2, PI3K, Akt and p-Akt were determined by Western blot. In order to confirm the regulatory effect of ROCKs on PI3K/Akt signaling pathway, the cells were divided into control group (5.5 mmol/L glucose), high glucose group (33 mmol/L glucose) and high glucose+Y27632 (ROCK inhibitor) group. Western blot was used to detect the protein levels of ROCK1, ROCK2, PI3K, Akt and p-Akt. RESULTSAfter 48 h of high glucose exposure, the values of relative cell viability in 33 and 40 mmol/L glucose groups were (79.71±2.43)% and (68.41±7.49)%, respectively, both of which were significantly decreased compared with normal control group (P<0.05). After 48 h of high glucose exposure, the relative mRNA levels of ROCK1 and ROCK2 in 33 and 40 mmol/L glucose groups were significantly increased compared with normal control group (P<0.05). Compared with normal control group, the apoptotic rate in 33 and 40 mmol/L glucose groups was increased significantly (P<0.05). Compared with normal control group, the protein expression of ROCK1, ROCK2 and cleaved caspase-3 in 33 and 40 mmol/L glucose groups was increased (P<0.05), while the protein expression of Bcl-2 was decreased (P<0.05). No significant difference in the protein levels of PI3K and Akt among the 3 groups was observed, while the protein level of p-Akt in 33 and 40 mmol/L glucose groups was decreased compared with normal control group (P<0.05). Compared with high glucose group, the expression of ROCK1 and ROCK2 was decreased in high glucose+Y27632 group. No significant difference in the protein levels of PI3K and Akt among the 3 groups was observed. Compared with normal control group, the protein level of p-Akt in high glucose group was decreased, and the protein level of p-Akt in high glucose+Y27632 group was increased significantly compared with high glucose group. CONCLUSION Under high glucose environment, ROCK may reduce the level of p-Akt by inhibiting the PI3K/Akt signaling pathway, thus promoting the apoptosis of cardiomyocytes.  相似文献