骆驼斯氏副柔线虫病间接ELISA检测方法的建立     

苏倩  冯陈晨  赵学亮  王金玲  王文龙  呼和巴特尔 《中国畜牧兽医》2019,46(6):1756-1763

为建立骆驼斯氏副柔线虫病间接ELISA （iELISA）诊断方法,本试验对骆驼斯氏副柔线虫半胱氨酸蛋白酶CPR基因进行重组表达,将获取的重组蛋白（rCPR）进行纯化和Western blotting检测,然后以纯化好的重组蛋白为抗原,通过棋盘滴定试验优化了抗原包被浓度、包被条件、抗体稀释度、酶标二抗稀释度、封闭液和封闭时间等,建立了骆驼斯氏副柔线虫病iELISA诊断方法,并对建立的iELISA检测方法进行了重复性、敏感性、特异性试验和临床检测。结果显示,抗原最佳包被浓度为8 μg/孔,血清最佳稀释度为1：50,酶标二抗最佳稀释度为1：5 000,最佳包被条件为4℃包被过夜,最佳封闭条件为3% BSA封闭2 h。临界值为0.235,待检血清D_{450 nm}值>0.235则确定为阳性。重复性试验中变异系数均<10%,重复性较好;用该方法检测阳性血清的敏感性为96.3%;此方法仅与骆驼斯氏副柔线虫病阳性血清发生特异性反应,与感染了其他寄生虫的阳性血清无交叉反应,特异性为100%;对140份临床血清进行检测,阳性率为86.4%。综上可知,本试验成功建立了一种快速有效诊断骆驼斯氏副柔线虫病的iELISA方法。  相似文献   

骆驼乳对疾病辅助治疗作用研究进展     

蔡树东  黄浩  阿里甫·努肉力  罗生金 《中国草食动物科学》2019,(6):52-55

骆驼乳是一种具有极高营养价值的乳品,不仅具有良好的保健作用,而且还具有一定的医用价值。文章重点对骆驼乳辅助治疗糖尿病、肝炎以及慢性肾功能衰竭的研究进展作一综述,并对骆驼乳辅助治疗疾病的应用前景进行了展望,为今后骆驼乳辅助治疗疾病提供参考。  相似文献   

Zoonotic origin and transmission of Middle East respiratory syndrome coronavirus in the UAE          下载免费PDF全文

Y. Tao  Y. M. Eltahir  E. A. Elsayed  B. A. Marzoug  O. K. A. Bensalah  A. I. Khalafalla  M. Al Mulla  A. Khudhair  K. A. Elkheir  Z. B. Issa  K. Pradeep  F. N. Elsaleh  H. Imambaccus  J. Sasse  S. Weber  M. Shi  J. Zhang  Y. Li  H. Pham  L. Kim  A. J. Hall  S. I. Gerber  S. Tong  S. S. M. Al Muhairi 《Zoonoses and public health》2018,65(3):322-333

Since the emergence of Middle East respiratory syndrome coronavirus (MERS‐CoV) in 2012, there have been a number of clusters of human‐to‐human transmission. These cases of human‐to‐human transmission involve close contact and have occurred primarily in healthcare settings, and they are suspected to result from repeated zoonotic introductions. In this study, we sequenced whole MERS‐CoV genomes directly from respiratory samples collected from 23 confirmed MERS cases in the United Arab Emirates (UAE). These samples included cases from three nosocomial and three household clusters. The sequences were analysed for changes and relatedness with regard to the collected epidemiological data and other available MERS‐CoV genomic data. Sequence analysis supports the epidemiological data within the clusters, and further, suggests that these clusters emerged independently. To understand how and when these clusters emerged, respiratory samples were taken from dromedary camels, a known host of MERS‐CoV, in the same geographic regions as the human clusters. Middle East respiratory syndrome coronavirus genomes from six virus‐positive animals were sequenced, and these genomes were nearly identical to those found in human patients from corresponding regions. These data demonstrate a genetic link for each of these clusters to a camel and support the hypothesis that human MERS‐CoV diversity results from multiple zoonotic introductions.  相似文献   

AGREEMENT BETWEEN A-MODE AND B-MODE ULTRASONOGRAPHY IN THE MEASUREMENT OF OCULAR DISTANCES     

Wali A.  Hamidzada  PhD  Ebi P.  Osuobeni  PhD 《Veterinary radiology & ultrasound》1999,40(5):502-507

It is generally accepted that A-mode ultrasonography is more accurate than B-mode in measuring intraocular dimensions. Consequently, A-mode ultrasonography is the procedure of choice in ocular biometry while B-mode ultrasonography is used principally for diagnostic purposes. In this study, we investigated the agreement between measurement of intraocular distances using A- and B-mode ultrasonography on freshly enucleated camel eyes. Our results suggest that relative to average A-mode values, B-mode overestimates corneal thickness (bias = 0.06 mm) and anterior chamber depth (bias = 0.03 mm), while it underestimates lens thickness (bias = -0.11 mm), vitreous chamber depth (bias = -0.32 mm) and axial length (bias = -0.40 mm). In general, difference between A- and B-mode values is larger for deeper intraocular dimensions. This implies that the two methods are more likely to give different readings for measurements of lens thickness, vitreous chamber depth and axial length.  相似文献   

双峰驼肘关节动脉分布     

王建林  崔胜  谢铮铭 《畜牧兽医学报》1999,30(1):91-96

作者研究了双峰驼肘关节的动脉分布情况。结果表明：分布于双峰驼肘关节的动脉支有肘前支、肘内侧支、肘外侧支和肘后支。肘前支有肘前内侧支、肘前上支、肘前中支和肘前下支，均自肘横动发出；肘内侧支自尺侧副动脉分出；肘外侧支有肘外侧上支、肘外侧中支和肘外侧下支，均自肘横动脉分出；肘后支有肘后上支、肘后中肘、肘后下支，肘后内侧支、肘后外侧上支和肘后外侧下支，除肘后上支和肘后外侧上支自肘横动脉分出外，其斜匀自尺侧  相似文献   

不同泌乳时间阿拉善双峰驼驼乳中氨基酸的含量变化     

赵电波  白艳红  吉日木图  张和平 《乳业科学与技术》2007,29(1):15-17

研究10峰阿拉善双峰驼第一次分娩后不同泌乳时间驼乳中氨基酸的含量变化。第一次所挤驼乳的蛋白中Thr、Met、Ile、Leu、Phe和Lys的含量分别为6．50、2．10、4．05、7．94、4．38和7．40g／100g，7d时驼乳蛋白中相应的值分别为4．54、2．86、5．50、9．46、4．40和7．89g／100g，之后含量保持相对稳定；而泌乳前1d的时间内必需氨基酸Val的含量较高，之后含量保持相对稳定（6．11—6．25g／100g）。分娩后第一次所挤初乳中Asp、Ser、Gly、Ala和Arg的含量分别为8．39、6．01、3．48、3．76和4．94g／100g，之后随泌乳时间的延长含量降低，到泌乳7d时分别为7．16、4．72、1．49、2．51和4．27g／100g，之后含量保持相对稳定。在研究的泌乳时间内乳样中His（2．50—2．75g／100g）和Phe（4．31-4．73g／100g）的含量没有大的变化。  相似文献   

双峰驼跖趾关节解剖结构   总被引：3，自引：0，他引：3  

孟昭权  谢铮铭 《中国兽医学报》1999,19(1):61-64

采用大体解剖学方法，研究了成年双峰驼的８个跖趾关节，并与马、牛、羊、猪、犬等动物的对等器官进行了比较。结果发现，双峰驼跖趾关节的形态结构有以下显著特征：（１）跖骨远端关节面后部有矢状嵴，近趾节骨近端关节面后部有与之相对应的矢状沟，这种结构与跖行动物和趾行动物相类似；（２）籽骨上没有对应近趾节骨近端的关节面；（３）籽骨间韧带只连接同一趾上的２个籽骨，与牛的将２趾的４个近籽骨连在一起的结构不同；（４）轴侧侧副韧带不像牛的那样与对侧的同名韧带共同起始于跖骨远端滑车间切迹，而是分别起始于此切迹的轴侧韧带窝及韧带结节；（５）轴侧侧副韧带仅为１层，而远轴侧侧副韧带分为２层；（６）籽骨直韧带、趾间趾节骨籽骨韧带及趾间近韧带均缺如。  相似文献   

The relationship between serum level of thyroid hormones,trace elements and antioxidant enzymes in dromedary camel (<Emphasis Type="Italic">Camelus dromedarius</Emphasis>)     

S. Nazifi  M. Mansourian  B. Nikahval  S. M. Razavi 《Tropical animal health and production》2009,41(1):129-134

Thyroid hormones might be able to regulate the activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GPX). The role of thyroid hormones in metabolic pathways and antioxidant enzyme activities are well known in many species. Nevertheless, there is no report describing probable relationship between thyroid hormones status, erythrocyte antioxidant enzymes and serum profiles of trace elements. This study was undertaken to investigate the relationship between these parameters in Iranian dromedary camels. Blood samples were taken from the jugular vein of 30 clinically healthy Iranian dromedary camels under aseptic conditions during 6 consecutive days of summer. The serum was analyzed for serum profile of thyroid hormones, trace elements, SOD and GPX activity. There were no significant differences in serum thyroid hormones, serum level of zinc (Zn), copper (Cu), manganese (Mn), iron (Fe), selenium and antioxidant enzymes in different days (P > 0.05). There was a significant negative correlation between SOD and Fe (P < 0.01, r = -493). There was no significant correlation between other parameters. In case of copper deficiency likewise the present study, the copper was used to produce more SOD, so there was a decrease in Fe transportation, and it might be a cause of decrease in Fe amount. On the other hand, a relatively small quantity of Fe is present in tissue myoglobin, catalase, peroxidases, and cytochromes. So it might be another cause of decrease in Fe amount. The explanation for these finding is not possible at this moment and further investigations are needed to interpret these changes.  相似文献   

Studies on the Respiratory Disease ‘Sonbobe’ in Camels in the Eastern Lowlands of Ethiopia     

Bekele T 《Tropical animal health and production》1999,31(6):333-345

New epidemics of respiratory disease have caused 29.6 morbidity and 6.4% mortality in camels in the Somalia region of Ethiopia. The major clinical signs observed were fever of 40–41.5°C, depression, cough, loss of appetite and a watery nasal discharge that became mucopurulent at a later stage. Finally, the camel became recumbent and extended its neck straight along the ground. Some of the animals died within 8–9 days. The major post-mortem lesions were hydrothorax, adhesion of the lung to the thorax, red and grey hepatization, emphysema, hydropericardium and fibrinous pericarditis. A treatment trial indicated that oxytetracycline was more effective than a combination of penicillin and streptomycin, the results showing a significant difference (p>0.05) between the treated and control groups. The bacteria isolated from lung, thoracic fluid and whole blood were Pasteurella haemolytica. Further studies on the epidemiology of this disease, the identification of the serotypes involved, and the demonstration of any primary viral initiating agent are recommended to allow the development of preventive methods.  相似文献   

Community Perceptions of Important Camel Diseases in Lapur Division of Turkana District,Kenya     

Mochabo KO  Kitala PM  Gathura PB  Ogara WO  Catley A  Eregae EM  Kaitho TD 《Tropical animal health and production》2005,37(3):187-204

This paper presents the results of a study conducted in Lapur Division of Turkana District, Kenya, to estimate the incidence and mortality of camel trypanosomosis using participatory methods. Four livestock camps ('adakars') were conveniently selected for the study. Four informant groups comprising 6 8 key persons were used for the participatory exercises. The camel diseases identified by the pastoralists in their order of importance according to annual incidence were: trypanosomosis (11.4%); mange (10.8%); tick infestation (7.9%); haemorrhagic septicaemia (7.7%); and non-specific diarrhoea (7.6%). Almost half (49.3%) of the camel population suffered from at least one disease over the previous year. The annual incidence and mortality rates of trypanosomosis were estimated at 15%, and 9.9%, in adult camels and 6.9% and 5.2%, in young camels, respectively. There was a seasonal occurrence of trypanosomosis, with most cases reported in the dry season. The prevalence levels of the disease reportedly declined from about 100%, in 1978 to an almost stable state of about 15% in 2002. This study revealed that camel trypanosomosis is still an important disease in Turkana District, exacting a heavy toll in terms of morbidity and mortality. The economic losses due to the disease were likely to have been great owing to the central role the camel plays in this arid district of Kenya.  相似文献