Reference values and repeatability of the Schirmer tear tests I and II in domesticated,clinically normal dromedary camels (Camelus dromedarius)          下载免费PDF全文

Mohamed A. Marzok  Adel M. Badawy  Sabry A. El‐khodery 《Veterinary ophthalmology》2017,20(3):259-265

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家养双峰驼SRY基因的序列分析与拷贝数研究     

陈慧玲  任战军  叶翔杨  赵纪萍  张成东 《西北农业学报》2017,26(11):1569-1576

为探究中国家养双峰驼SRY基因的序列以及群体间拷贝数变异,对7个中国家养双峰驼群体共94个个体的SRY基因部分序列进行测序,分析SRY基因序列与其他物种的相似性;并且以CYP2A基因为内参基因,运用实时荧光定量PCR技术检测SRY基因的拷贝数。结果表明,中国家养双峰驼与人、马、猪、羊及普通牛的SRY基因相似度分别为67.7%~68.1%、68.5%~69.4%、73.8%~74.4%、73.8%~74.8%、74.1%~74.8%。在家养双峰驼SRY基因的多个拷贝中,除1个拷贝外,其余拷贝的HMG序列与羊驼、人、马、猪、绵羊及普通牛的HMG序列相比,相似度分别为97.3%、84.2%、85.6%、86.3%、87.0%、85.6%。SRY基因在中国家养双峰驼Y染色体上以多拷贝形式存在,变幅为1~9个拷贝。表明中国家养双峰驼SRY基因的HMG序列是高度保守的,而且该基因属于多拷贝基因。  相似文献   

中国5个地区家养双峰驼的遗传多样性研究   总被引：1，自引：0，他引：1  

王乐  任战军  程佳  王咏絮  王洪亮 《西北农业学报》2010,19(11):18-23

为了科学评价、保护和利用骆驼遗传资源,采用10个微卫星位点对40峰阿拉善骆驼、40峰河西骆驼、60峰北疆骆驼、60峰东疆骆驼和60峰南疆骆驼共260个个体进行群体间、群体内遗传水平变化研究,并对群体结构进行遗传多样性分析。检测得到129个等位基因。5个群体的多态信息含量(PIC)为0.578~0.665,观察杂合度(Ho)的范围为0.187~0.261,期望杂合度的变化范围为0.612~0.699。依据Da遗传距离构建的UPGMA系统发生树表明,5个地区家养双峰驼可以分为两个分支,阿拉善骆驼、河西骆驼、东疆骆驼聚为一支;北疆骆驼和南疆骆驼聚为一支。结果表明,河西骆驼和阿拉善骆驼显示了最密切的关系,而阿拉善骆驼和南疆骆驼之间有最远的遗传距离。  相似文献   

Relevance of Dried Meat Product （Kundi）, an Intermediate Moisture Meat,for Food Security     

P.  O.  Fakolade 《农业科学与技术》2011,(4):563-569

The main goal of food security is for individual to be able to obtain adequate and quality food needed at all times, and to utilise the food to meet the body needs. Kundi is a relish Intermediate Moisture Meat （dried meat） product, produced in the Northern part of Nigeria. 2-3 years old male Camelus dromedarius and White Fulani animals were used for this study, 2 kg of semimembranous muscles from each animal were used, and trimmed of all external fat, connective tissues and bones. Meat samples were cut in sizeable pieces of weight ranges 70-90 grams of 6-8 cm and kept in the refrigerator for 24 hours. Fresh meat cuts were boiled for 20 minutes at 100 ~C, seasoned and oven dried for 3 hours at 170 ~C. This study assessed the proximate composition of fresh and dried meat （Kundi） products and their sensory evaluation in a completely randomized design. The results showed that fresh camel meat had 74.55% and 21.96% significantly （P 〈 0.05） higher in moisture and protein content than 72.69% and 18.96% of fresh beef, while fresh beef had higher （P 〈 0.05） ether extract （6.34%） than fresh camel meat （2.39%）. Laboratory Prepared Beef Kundi （LPBK） had the highest （P 〈 0.05） value in moisture content 35.09% followed by Laboratory Prepared Camel ＇Kundi＇ （LPCK） with 33.21% while Commercial Kundi （CK） had the least. Protein obtained was inversely proportionally to moisture content. Ether extract for LPCK and LPBK were statistically similar （P 〉 0.05） and both were lower （P 〈 0.05） than value obtained for CK. The results obtained for sensory evaluation showed that the panelist rated seasoned Kundi to have the highest significant （P 〈 0.05） valve for tenderness, flavour, colour, juiciness, texture and acceptability with values 6.50, 5.30, 6.50, 6.53, 6.30 and 7.00 respectively. Study showed that dry meat products （Kundi） is of high nutritional qualities, and could be of a great relevance; to food security.  相似文献   

新疆双峰驼C3d基因cDNA的克隆与序列分析     

娜斯拜·阿卜杜瓦哈普  高晓娟  坤杜孜阿依·阿布都沙拉木  李江伟 《新疆农业科学》2023,60(2):493-500

【目的】克隆骆驼补体C3d基因,分析序列特征,为其佐剂效应研究提供依据。【方法】利用Trizol试剂,从骆驼肝脏组织提取总RNA,并反转录获得cDNA。设计C3d特异引物,应用PCR技术扩增C3d序列,构建到pMD18-T载体。将重组质粒转入感受态菌株并双酶切鉴定。使用ClustW,DNAstar, Swiss-Model软件对构建的重组C3d基因序列进行同源性比对,建立系统进化树并对其二级和三级结构进行预测和分析。【结果】采用骆驼C3d特异引物,PCR扩增获得大小为909 bp的骆驼补体C3d基因。构建至T载体并转入大肠杆菌,获得阳性转化克隆。克隆的新疆双峰驼C3d序列与骆驼科C3d基因之间的同源性在97%以上,与牛和猪C3d基因同源性为88%,与兔和人的C3d基因同源性为83%。对骆驼C3d蛋白采用Swiss-Model软件进行序列分析,预测该蛋白质的二级结构主要由α-螺旋和β-折叠组成,该蛋白可能具有良好的免疫原性和免疫结合活性。【结论】C3d基因在进化中较为保守,在骆驼免疫中可以采用亲缘关系较近的其他动物来源的C3d作为分子佐剂从而增强骆驼免疫效果。  相似文献   

阿拉善双峰驼及驼乳、驼肉研究概述     

杨永青  王文曦  李国银  张欣昕  聂晶  莎娜  史培 《畜牧与饲料科学》2021,42(2):109-113

阿拉善双峰驼是世界主要骆驼品种之一,是宝贵的骆驼种质资源。文章综述了阿拉善双峰驼的驯养历史和驼乳、肉产品的研究概况,结合调研和测定数据分析了驼肉中氨基酸的成分及含量,以期为保护阿拉善双峰驼种质资源以及深入开发双峰驼系列产品提供帮助。  相似文献   

双峰幼驼组织中矿物元素含量的分布     

刘宗平  曲亚玲  马小军  张尤嘉  杨得兵 《动物医学进展》2001,22(2):83-84

对双峰幼驼及胎儿组织矿物元素的含量进行了分析。结果表明，所测样品矿物元素的分布规律与成年双峰驼基本一致，幼驼和成年驼组织元素含量无明显差异，而胎儿组织Cu含量显著低于幼驼和成年驼，肝脏Zn含量则较高。  相似文献   

Superovulatory Response and Embryo Recovery after Treatment with Different Gonadotrophin during Induced Luteal Phase inCamelus dromedarius     

Vyas S  Rai AK  Goswami PK  Singh AK  Sahani MS  Khanna ND 《Tropical animal health and production》2004,36(6):557-565

Superovulation, embryo recovery and transfer were attempted in 19 dromedary camels of about 6-10 years of age, and having calved at least once. Superovulation was done using two commercially available porcine FSH preparations, FSH-I (II donors) and FSH-2 (8 donors) during a luteal phase created by inducing ovulation with hCG. The superovulatory response was assessed by ultrasonography. The embryo recovery was attempted non-surgically in sitting position on day 8 and day 7 after first mating in one FSH-1 and one FSH-2 group, respectively. Considerable individual variation in response to the superovulatory stimulus was observed. No significant difference was observed between the two groups in terms of superovulatory response and embryo recovery (p > 0.05). In total 30 embryos were recovered from 17 donors (1.51 embryos/donor). Recipients were synchronized with donors using hCG. Eight embryos were transferred, resulting in two pregnancies and live births.  相似文献   

The Nerve Supply to the Orbit of the Bactrian Camel     

Cui S  Wang J  Xie ZM 《Veterinary research communications》2004,28(1):7-15

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Separation and Purification of Ovulation-inducing Factors in the Seminal Plasma of the Bactrian Camel (Camelus bactrianus)     

Li X  Zhao X 《Veterinary research communications》2004,28(3):235-245

Ovulation in the Bactrian camel depends upon ovulation-inducing factors in the seminal plasma. The present study was conducted to isolate and purify the bioactive fractions from the seminal plasma of these camels. The seminal plasma was fractionated by anion-exchange chromatography, and six fractions were obtained. The bioactive potential of each fraction was estimated from its effect on rat pituitary tissue cultured in vitro and by the effect of an intramuscular injection of the fraction into female camels in vivo. Both the third fraction (F3) and the fifth fraction (F5) stimulated the release of LH in vitro and in vivo. In addition, female camels ovulated within 48 h after intramuscular injection of F3. However, neither F3 nor F5 had any significant effect on the secretion of FSH, either in vitro or in vivo. When F3 was further fractionated into four subfractions, the third subfraction (F3-3) still stimulated the in vitro release of LH, but not of FSH. An attempt to further purify the ovulation-inducing factors in F3-3 failed owing to the similarity of the molecular characters.  相似文献