Leaf, floret and seed infection of wheat by Pyrenophora semeniperda     

M. A. Campbell  R. W. Medd †‡ 《Plant pathology》2003,52(4):437-447

Infection processes of Pyrenophora semeniperda on seedling and adult wheat leaves and wheat ears were investigated. Almost 100% germination of conidia occurred on seedling leaves, compared with 20–30% on adult leaves. Appressoria formed over the anticlinal epidermal cell walls and haloes always accompanied infection. Sometimes papillae formed within the leaves as a resistance mechanism. Infection hyphae ramified through the intercellular spaces of the mesophyll resulting in cellular disruption. The infection processes on floral tissues were similar to those observed on leaves; however, no infection occurred on anther, stigmatic or stylar tissues. Infection of ovarian tissue occurred both with and without appressoria formation. Hyphae grew mainly in the epidermal layers and appeared unable to breach the integumental layer as no growth was observed in endosperm or embryo tissues. The optimum dew period temperature for conidial germination was 23·6°C, compared with 19·9°C for lesion development, 20·4°C for the production of infection structures on seedling leaves and 23·7°C for floret infection. Leaf disease development occurred in a logistic manner in response to dew period, with maximum infection observed after 21 h compared with > 48 h in seeds. An initial dark phase during the dew period was necessary for infection and temperature after the dew period had an effect, with significantly more numerous and larger lesions being formed at 15°C compared with 30°C. Seedling leaves were found to be more susceptible than older leaves, under both field and controlled environment conditions. Infection of wheat seeds following inoculation of ears, or after harvest burial of inoculated disease-free seeds, was demonstrated. In the latter, 3-week-old seedlings were slightly stunted, whereas older plants were unaffected. The apparent unimportance of this plant pathogen as a cause of leaf disease in relation to its poor adaptation to dew periods and dew period temperature is discussed, along with the importance of its seed borne characteristics.  相似文献   

Microscopic detection of reactive oxygen species generation in the compatible and incompatible interactions of Alternaria alternata Japanese pear pathotype and host plants     

Takeshi Shinogi  Tomoko Suzuki  Takayuki Kurihara  Yoshihiro Narusaka  Pyoyun Park 《Journal of General Plant Pathology》2003,69(1):7-16

 Reactive oxygen species (ROS) generation was examined in the interaction of Alternaria alternata Japanese pear pathotype and host plants using three methods: nitro blue tetrazolium (NBT) method for microscopic detection of O₂ ⁻, diaminobenzidine (DAB) methods for microscopic detection of H₂O₂, and cerium chloride methods for ultrastructural detection of H₂O₂. ROS generation was detected by NBT and DAB methods at appressoria on leaves of susceptible cultivars and heat-shocked leaves of resistant cultivars but not in leaves of resistant cultivars. Ultrastructural detection by the cerium chloride method identified ROS generation at cell walls of appressoria and penetration pegs in susceptible, resistant leaves and heat-shocked leaves. These differences in the ultrastructural and microscopic data in resistant areas were due to the restriction of ROS generation in limited areas, the side facing the plant surface, of appressoria and penetration pegs. Therefore, ROS generation was apparently induced regardless of the resistance or susceptibility of the cultivar with the difference being in the volumes generated. After evaluating the pathological role of ROS generation in fungal structures, such generation was found to be associated with early penetration of cell walls in pear plants. Additionally, ROS generation in plants was also found in degrading pectin layers near infected hyphae and in plasma membrane modification sites in susceptible leaves but not in resistant leaves. ROS generation in susceptible leaves might be accompanied with plasma membrane damage, although the role of ROS generation in the pectin layers is not clear. ROS generation in both fungal and plant cells during their interaction was likely associated with the expression of susceptibility. Received: June 3, 2002 / Accepted: July 31, 2002  相似文献   

A new approach to evaluate relative resistance and tolerance of tomato cultivars to begomoviruses causing the tomato yellow leaf curl disease in Spain   总被引：1，自引：0，他引：1  

L. Rubio †  J. R. Herrerob  J. Sarrió  P. Morenoa  J. Guerria 《Plant pathology》2003,52(6):763-769

A simple procedure to evaluate relative resistance and tolerance of tomato cultivars to the begomoviruses causing tomato yellow leaf curl (TYLC) disease in Spain was developed. To estimate the resistance and tolerance levels of a cultivar, several formulae were developed based on the ratio of infected plants, virus titre (estimated by tissue–print hybridization) and symptom intensity. The formulae were applied to five commercial tomato cultivars (Amoretto, Birloque, Royesta, Tovigreen and Ulises) naturally infected by TYLC viruses. The analyses showed that Ulises, Birloque and Tovigreen exhibited a moderate resistance, and Ulises was also highly tolerant. There was a positive correlation between symptom intensity and virus titre in infected plants, suggesting that the hybridization technique could also be used as an early estimator of tolerance. Finally, molecular hybridization and nucleotide sequence analyses of the begomovirus intergenic region showed that the local TYLC virus population consisted of a single species, Tomato yellow leaf curl virus (TYLCV, formerly TYLCV-Israel), with low genetic variation (nucleotide identity between isolates higher than 97%).  相似文献   

引起糖甜菜细菌性叶斑病的萎蔫短小杆菌新致病变种   总被引：3，自引：0，他引：3  

陈永芳  郭坚华  方中达 《植物病理学报》2003,33(5):396-400

 1995年在内蒙古临河市新发现了糖甜菜细菌性叶斑病,从病斑所分离的10个细菌菌株经柯赫氏法则验证,均确系该病的病原菌。采用形态观察、表型特征和生理生化特性测定、数值分析、血清学反应、细胞化学成分分析、DNA G+C mol%和DNA-DNA同源性测定进行了鉴定,并与植物病原棒形细菌15个标准菌株进行了比较。该病原菌为革兰氏阳性细菌,不规则短杆状,有一根鞭毛、亚极生或侧生,结合其生理生化特性、细胞化学成分和DNA G+C mol%和DNA-DNA同源性测定结果,认为应属于短小杆菌属(Curtobacterium)的萎蔫短小杆菌(Cur. flaccumfaciens),数值分析也支持这一结论。此外,据血清学反应结果及其对短小杆菌属的其它植物寄主的致病情况,认为该病原菌应是萎蔫短小杆菌种下的一个新的致病变种,定名为Curtobacterium flaccumfaciens pv. beticola pv. nov. Chen et al.,2000(萎蔫短小杆菌糖甜菜致病变种)。  相似文献   

采自哈纳斯的新疆新记录的地衣   总被引：8，自引：1，他引：8  

阿布都拉  吴继农 《干旱区研究》1997,14(4):1-4

报道了１９９６年７月在哈纳斯自然保护区及其附近所采到的新疆新记录地衣１２种，其中１种１属为地知地衣，另１１种隶属于１１个属，６科。  相似文献   

种植方式与种植密度对大力士高粱的影响   总被引：1，自引：0，他引：1  

牟芝兰 《四川草原》2004,(3):27-28

本试验用裂区设计,研究了大力士高粱在16种不同种植密度、3种种植方式下,产草量、生产速度、茎叶比、茎粗的变化情况。结果表明,在撒播密度为18 kg/hm2、条播为22.5 kg/hm2、穴播为28.5 kg/hm2时达到最高产量;生长速度在第53-55 d时最高,达5.7 cm/d;茎叶比随着产量的增加逐渐减少;茎粗随着种植密度的增大而有减小的趋势。  相似文献   

外来入侵种的危害与防治对策   总被引：1，自引：0，他引：1  

付秀琴 《四川草原》2004,(7)

外来入侵种对当地的自然生态系统结构和功能以及生物多样性都有着深远的影响,而且可能对地方的生态环境和经济造成严重危害。四川已经成为遭受外来入侵种威胁的省份之一,全省现有20多个外来入侵种。为了防止外来入侵种的泛滥,应当建立外来物种的引入评价机制,慎重进行有意引种;加强口岸管理,防止外来入侵种通过贸易途径无意引入,同时采取措施控制已经产生危害的外来入侵种。  相似文献   

草莓摘叶处理对果实芳香物质的影响   总被引：3，自引：0，他引：3  

姜远茂  彭福田  刘松忠  束怀瑞  李可昌 《园艺学报》2004,31(2):230-232

 对草莓(Fragaria×ananassa Duch．)‘哈达’品种坐果后植株进行摘叶处理，对成熟果实的糖类和芳香物质含量进行了测定分析，结果表明：不摘叶、摘1/3叶和摘2/3叶3个处理果实GC/MS分析分别检测出43、33和37种芳香物质成分。随摘叶程度加重，芳香物质成分中酯类的相对含量呈下降趋势，而醛类的相对含量呈上升的趋势；2，5-二甲基-4-甲氧基-3(2H)-呋喃酮的相对含量明显降低；果糖和总糖含量显著降低。  相似文献   

农业外来生物入侵种研究现状与发展趋势   总被引：9，自引：0，他引：9  

张润志  张大勇  叶万辉  桑卫国  薛大勇  李尉民 《植物保护》2004,30(3):5-9

目前国际上对外来生物入侵种的研究主要在个体水平上研究外来种对新环境的进化与适应,本地种对生物入侵后发生的进化调整;种群和群落水平上研究外来种的定居、潜伏、竞争和暴发;生态系统水平上研究生境的可入侵性和外来种对生态系统结构和功能的影响等几个方面。风险分析标准化与定量化、检疫鉴定快速化、除害技术安全化、信息管理全球化是全世界对外来物种入侵管理与科研的焦点问题。本文依据外来物种入侵的形成和扩散暴发过程,提出了超前预防和综合管理等未来需要解决的关键问题。  相似文献   

半自然条件下几种赤眼蜂及品系对亚洲玉米螟卵寄生能力比较   总被引：1，自引：0，他引：1  

张帆  王素琴  张君明  孟昭君  李德刚 《植物保护》2004,30(4):29-32

在半自然条件(田间网罩)下,将人工饲养的玉米螟卵按4、8、16块/株3个处理分别固定在网罩内的玉米植株中上部叶片上,然后每网罩分别引入供试赤眼蜂20头,24 h后更换玉米螟卵块,连续3 d。7 d后调查供寄生的玉米螟卵被寄生率及羽化率。结果表明:在半自然条件下广赤眼蜂伊朗1-1品系虽寄生能力高于其他供试品系,但到第2、3天明显降低;玉米螟赤眼蜂北京6-2-2品系每天均维持一个较平稳的寄生能力,产卵量在时间上的分配比较分散,并在第2、3天显示出比其他品系较高的寄生潜能。广赤眼蜂伊朗1-1品系的卵块寄生数量可随卵块密度的增加而增加,而其他品系则在各处理密度下,寄生数量基本没有变化;从卵粒寄生率看,广赤眼蜂伊朗1-1和吉林1-2两个品系的寄生数量随卵块密度的增加而大幅度提高,其他品系增加幅度较小。  相似文献